ABSTRACT
We report here the identification of a novel DRB4*01 allele, DRB4*010304, found in a patient waiting for a liver transplantation. The new allele was detected during a routine DNA-based HLA typing. Sequencing confirmed that the new allele is identical to DRB4*01030101 at exon 2 except for position 216 where the new allele has a T instead of a C.
Subject(s)
Alleles , HLA-DR Antigens/genetics , Base Pair Mismatch , Base Sequence , Codon , Exons , HLA-DRB4 Chains , Histocompatibility Testing , Humans , Liver Transplantation , Molecular Sequence Data , Polymorphism, Genetic , Sequence Alignment , Sequence Analysis, DNAABSTRACT
The frequency of HLA alleles at HLA-DR and DQ loci, and that of the related HLA-D specificities, were estimated in the Italian population. 109 healthy unrelated subjects, born in several Italian regions and living in the district of Torino, were studied. DNA typing was achieved by the restriction fragment length polymorphism (RFLP) analysis of HLA-DR beta, DQ alpha and beta genes, hybridizing specific probes with TaqI digested DNAs. The present study allowed to define in more detail the HLA class II polymorphisms in the Italian population.
