Canine induced pluripotent stem cells can be successfully maintained in weekend-free culture systems.

Canine induced pluripotent stem cells (ciPSCs) can provide useful insights into novel therapies in both veterinary and medical fields. However, limited accessibility to the present culture medium and requirement of considerable time, effort, and cost for routine ciPSC maintenance restrict advancement in ciPSC research. In addition, it is unknown whether ciPSC culture conditions influence differentiation propensity. We investigated the availability of the common human pluripotent stem cells (hPSCs) culture systems for ciPSC maintenance and the differentiation propensities of the ciPSCs maintained in these culture systems. StemFlex and mTeSR Plus supported PSC-like colony formation and pluripotency markers expression in ciPSCs even after five passages. Additionally, ciPSCs were maintained under weekend-free culture conditions with a stable growth rate, pluripotency marker expression, and differentiation abilities using vitronectin (VTN-N) and Geltrex. Following maintenance of spontaneously differentiated ciPSCs under various conditions by embryoid body formation, there were few differences in the differentiation propensities of ciPSCs among the tested culture conditions. Thus, ciPSCs were successfully cultured under weekend-free conditions for ciPSC maintenance using StemFlex or mTeSR Plus with VTN-N or Geltrex. The present study offers simpler and more effort-, time-, and cost-saving options for ciPSC culture systems, which may lead to further development in research using ciPSCs.

Nowcasting Japan's GDP.

This paper backtests a nowcast of Japan's real GDP growth. It has three contributions: (i) use of genuine real-time data, (ii) implementation of a new method for the revision analysis that relates the revision of the nowcast to not only new observations but also data revisions, and (iii) a benchmarking of the nowcast to a market consensus forecast at monthly forecasting horizons. Our nowcast's forecast accuracy is comparable to that of the consensus at most, but not all, monthly horizons. Our revision analysis of the March 2011 earthquake finds the nowcast reacting to a steep post-quake decline in car production. In contrast, the consensus hardly budged, most likely because the decline was correctly viewed as temporary. The onset of COVID-19 triggers the consensus to take a precipitous descent. The nowcast, despite timely red flags from "soft" (i.e., survey-based) indicators, does not respond immediately in full, because it took a month or more for "hard" (i.e., non-survey-based) indicators to register sharply reduced economic activities.