ABSTRACT
Una mujer de 76 años fue remitida a nuestro Centro de Medicina Nuclear para someterse a 18F-FDG PET/TAC con sospecha clínica de vasculitis. La PET de cuerpo entero resultó negativa para vasculitis pero mostró hipermetabolismo moderado en varios ganglios linfáticos del mediastino y una captación intensa en las glándulas parótidas. Dado que la paciente refirió en anamnesis lesiones eritematosas de la piel en ambas piernas, realizamos una adquisición de PET de las extremidades inferiores que mostró una captación difusa en la región perimaleolar en ambas piernas. Sobre la base de los hallazgos de la PET/TAC que sugirieron sarcoidosis la paciente fue sometida a un lavado broncoalveolar (BAL) y a una biopsia de un ganglio linfático mediastínico que confirmó la sospecha de sarcoidosis(AU)
A 76-year-old woman was referred to our centre to perform 18F-FDG PET/CT with the clinical suspicion of vasculitis. Whole body PET was negative for vasculitis but it depicted moderate hypermetabolism in several lymph nodes of the mediastinum and intense uptake of the tracer in the parotid glands. Since the patient referred skin lesion on both legs a particular acquisition of the lower extremities was performed which showed diffuse uptake on the perimalleolar region of both legs. On the basis of the PET/CT findings that were suggestive for sarcoidosis the patient performed bronchoalveolar lavage (BAL) and biopsy of a mediastinal lymph node which confirmed the suspicion of sarcoidosis(AU)
Subject(s)
Humans , Female , Middle Aged , Sarcoidosis , Erythema Nodosum/complications , Erythema Nodosum , Fluorodeoxyglucose F18 , Bronchoalveolar Lavage/methods , Bronchoalveolar Lavage , Sentinel Lymph Node Biopsy/methods , Sentinel Lymph Node Biopsy , Sarcoidosis/complications , Sarcoidosis/diagnosis , Nuclear Medicine/methods , Nuclear Medicine/organization & administration , Vasculitis/complications , Mediastinum/pathology , Mediastinum , Parotid Gland/pathology , Parotid GlandABSTRACT
Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of α-satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and α-satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes.
Subject(s)
Centromere Protein B/metabolism , Centromere/metabolism , DNA, Satellite/metabolism , Cell Line, Tumor , Centromere/genetics , Centromere Protein B/genetics , Chromatin/genetics , Chromatin/metabolism , Chromosomes, Human, Pair 17/genetics , Chromosomes, Human, Pair 17/metabolism , DNA Probes/metabolism , DNA, Satellite/genetics , Fluorescent Antibody Technique , Humans , In Situ Hybridization, Fluorescence/methods , Lymphocytes/cytology , Metaphase , Microscopy, Atomic Force , Tissue Fixation/methodsABSTRACT
No disponible
Subject(s)
Humans , Female , Adult , Hypertension, Pregnancy-Induced/physiopathology , Hypertension, Pregnancy-Induced , Pheochromocytoma/complications , Pheochromocytoma , Suppression, Genetic/physiology , Suppression, Genetic/radiation effects , Positron-Emission Tomography , Fluorodeoxyglucose F18 , Cholelithiasis/complications , Cholelithiasis , Amlodipine/therapeutic use , Propranolol/therapeutic useABSTRACT
A 76-year-old woman was referred to our centre to perform (18)F-FDG PET/CT with the clinical suspicion of vasculitis. Whole body PET was negative for vasculitis but it depicted moderate hypermetabolism in several lymph nodes of the mediastinum and intense uptake of the tracer in the parotid glands. Since the patient referred skin lesion on both legs a particular acquisition of the lower extremities was performed which showed diffuse uptake on the perimalleolar region of both legs. On the basis of the PET/CT findings that were suggestive for sarcoidosis the patient performed bronchoalveolar lavage (BAL) and biopsy of a mediastinal lymph node which confirmed the suspicion of sarcoidosis.
Subject(s)
Erythema Nodosum/complications , Fluorodeoxyglucose F18 , Multimodal Imaging , Positron-Emission Tomography , Sarcoidosis/complications , Sarcoidosis/diagnosis , Tomography, X-Ray Computed , Aged , Female , Humans , Incidental Findings , RadiopharmaceuticalsSubject(s)
Adrenal Gland Neoplasms/diagnosis , Fluorodeoxyglucose F18 , Multimodal Imaging , Pheochromocytoma/diagnosis , Positron-Emission Tomography , Pregnancy Complications, Neoplastic/diagnosis , Radiopharmaceuticals/therapeutic use , Adrenal Gland Neoplasms/genetics , Adrenal Gland Neoplasms/surgery , Adult , Diastole , Female , Humans , Hypertension, Pregnancy-Induced/genetics , Hypertension, Pregnancy-Induced/physiopathology , Mutation , Pheochromocytoma/genetics , Pheochromocytoma/surgery , Pregnancy , Pregnancy Complications, Neoplastic/genetics , Pregnancy Complications, Neoplastic/surgery , Tomography, X-Ray ComputedABSTRACT
The nanoindentation-induced phase transformation behavior of silicon at elevated temperatures (25-150 degrees C) has been studied. Nucleation of Si-III/Si-XII on unloading is enhanced with increasing temperature and at the highest temperatures in an amorphous Si matrix, occurs in a continuous fashion without a pop-out event. Interestingly, for slow unloading at the highest temperatures, formation of Si-III/Si-XII in a crystalline Si matrix was not observed. Elevated temperatures enhance the nucleation of Si-III and Si-XII during unloading but the final composition of the phase transformed zone is also dependent on the thermal stability of the phases in their respective matrices.
