Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 11 de 11
Filter
Add more filters










Publication year range
1.
Mol Breed ; 42(6): 35, 2022 Jun.
Article in English | MEDLINE | ID: mdl-37312967

ABSTRACT

Chocolate spot (CS), caused by Botrytis fabae Sard., is an important threat to global faba bean production. Growing resistant faba bean cultivars is, therefore, paramount to preventing yield loss. To date, there have been no reported quantitative trait loci (QTL) associated with CS resistance in faba bean. The objective of this study was to identify genomic regions associated with CS resistance using a recombinant inbred line (RIL) population derived from resistant accession ILB 938. A total of 165 RILs from the cross Mélodie/2 × ILB 938/2 were genotyped and evaluated for CS reactions under replicated controlled climate conditions. The RIL population showed significant variation in response to CS resistance. QTL analysis identified five loci contributing to CS resistance on faba bean chromosomes 1 and 6, accounting for 28.4% and 12.5%, respectively, of the total phenotypic variance. The results of this study not only provide insight into disease-resistance QTL, but also can be used as potential targets for marker-assisted breeding in faba bean genetic improvement for CS resistance. Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-022-01307-7.

2.
J Hum Nutr Diet ; 33(5): 678-685, 2020 10.
Article in English | MEDLINE | ID: mdl-32424967

ABSTRACT

BACKGROUND: Vitamin A plays an important role in vision, cellular differentiation, embryonic development, reproduction, growth and the immune system. Women who live in developing countries face a risk of undernutrition during pregnancy as a result of poverty, poor diet quality and quantity, and a high fertility rate. This poor dietary problem could reflect the high risk of vitamin A deficiency in women. The present study aimed to determine the adequacy of vitamin A among pregnant women following antenatal care in health facilities of Dessie Town, Ethiopia, January 2017. METHODS: Health facility-based cross-sectional study was conducted among 390 women who attended antenatal care in Dessie Town. Food groups from the Food and Agriculture Organization based on 24-h dietary recall were used to measure dietary intake of vitamin A and dietary diversity of women. Adequacy of vitamin A was determined from the nutrient adequacy ratio after obtaining reports of nutrient intake from food composition tables version III and IV in terms of B carotene and retinol equivalent, respectively, based on the estimated average requirement recommendation of vitamin A, 370 retinol equivalent day-1 for pregnant women. Multivariable logistic regression analysis was performed to identify associated factors of vitamin A adequacy. RESULTS: Adequacy of vitamin A among pregnant women was 41.8%, with an average nutrient adequacy ratio of 0.9. The mean dietary intake of vitamin A was 290.1 µg day-1 . The predictors for adequacy of vitamin A were high and medium women diversity scores (adjusted odds ratio = 2.92; 95% confidence interval = 1.50-5.70) and (adjusted odds ratio = 1.87; 95% confidence interval = 1.11-3.16). CONCLUSIONS: In the present study, adequacy of vitamin A was low and was affected by the dietary diversity score. A focus on food-based approaches, especially regarding educating pregnant women to diversify their diet, is crucial for reducing the risk of vitamin A deficiency. Vitamin A is crucial micronutrient for the health of women and fetus, being essential for morphological, ocular and functional developments, as well as fetal organ and skeletal growth. Its requirement is greater during pregnancy and its deficiencies lead to maternal and child mortality and development. The dietary intake of vitamin A among pregnant women remains below the current recommendation. Inadequate intake of vitamin A Pregnant women Heath facility Dessie, Ethiopia.


Subject(s)
Diet, Healthy/statistics & numerical data , Prenatal Care/statistics & numerical data , Vitamin A/analysis , Adult , Cross-Sectional Studies , Diet Surveys , Ethiopia , Female , Health Facilities/statistics & numerical data , Humans , Odds Ratio , Pregnancy , Pregnancy Complications/prevention & control , Vitamin A Deficiency/prevention & control
3.
Neurology ; 67(8): 1458-60, 2006 Oct 24.
Article in English | MEDLINE | ID: mdl-16971696

ABSTRACT

Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a multisystemic autosomal recessive disease due to primary thymidine phosphorylase (TP) deficiency. To restore TP activity, we performed reduced intensity allogeneic stem cell transplantations (alloSCTs) in two patients. In the first, alloSCT failed to engraft, but the second achieved mixed donor chimerism, which partially restored buffy coat TP activity and lowered plasma nucleosides. Thus, alloSCT can correct biochemical abnormalities in the blood of patients with MNGIE, but clinical efficacy remains unproven.


Subject(s)
Gastrointestinal Diseases/blood , Gastrointestinal Diseases/surgery , Mitochondrial Encephalomyopathies/blood , Mitochondrial Encephalomyopathies/surgery , Nervous System Diseases/blood , Nervous System Diseases/surgery , Stem Cell Transplantation , Adult , Female , Humans , Male , Nucleosides/blood , Thymidine Phosphorylase/blood , Transplantation Chimera , Transplantation, Homologous , Treatment Failure
4.
Trop Anim Health Prod ; 36(7): 693-701, 2004 Oct.
Article in English | MEDLINE | ID: mdl-15563030

ABSTRACT

An investigation was made into coccidiosis of 190 scavenging indigenous chickens between September 2000 and April 2001 in three selected agroclimatic zones, in central Ethiopia. This was done through clinical, postmortem and microscopic examinations. Data were processed by chi-square and Mantel-Haenzel test. The study indicated that 25.8% (49/190) of the chickens were infected with coccidiosis and found to harbour one to four different species of Eimeria. Of these infected chickens, 30 (15.8%) and 19 (10.0%) were positive for clinical and sub-clinical coccidiosis, respectively. There was a significant altitude difference (chi2 = 14.7, p <0.001) in coccidiosis prevalence: 42.2% in chickens from highland region followed by 21.5% in mid-altitude and 13.1% in low-altitude areas. When quantified, the prevalence of coccidiosis was 2.66 and 4.83 times higher in the high-altitude than in mid-altitude (odds ratio, OR = 2.66, p<0.05) and low-altitude (OR = 4.83, p<0.001) chickens. The pathogenic Eimeria species responsible for clinical coccidiosis were E. necatrix, E. acervulina, E. maxima and E. tenella. With increasing demand for poultry products in developing countries, knowledge of production constraints in traditional management practices could help devise control strategies for constraints on backyard poultry production systems.


Subject(s)
Altitude , Chickens/parasitology , Coccidiosis/veterinary , Eimeria/isolation & purification , Poultry Diseases/epidemiology , Animals , Coccidiosis/epidemiology , Consumer Product Safety , Cross-Sectional Studies , Ethiopia/epidemiology , Female , Food Parasitology , Humans , Male , Prevalence , Rural Health
5.
J Immunoassay Immunochem ; 24(1): 57-72, 2003.
Article in English | MEDLINE | ID: mdl-12680607

ABSTRACT

A cross-sectional study was conducted on extensively reared chickens of three selected agro-climatic zones in Central Ethiopia to examine the predisposing effect of gastro-intestinal helminthes to intestinal Mycobacterium avium when it occurs as co-infection. This was done through a Lymphocyte Stimulation Test (LST) using avian PPD on peripheral blood mononuclear cells obtained from the blood of chickens and gross examination of digestive tract for the presence of helminth parasites. Data were analyzed using the statistical softwares SAS (1994) and Intercooled STATA version 6. Fourteen (14.7%) out of the 95 examined chickens were positive in in vitro LST showing stimulation index (SI) > or = 2. There was a significant (chi2 = 9.93, P < 0.01) difference in prevalence of M. avium by altitude: highest in chickens from lowland (27.8%) areas, followed by 13.3% in chickens from mid altitude and none was reacted to LST from highland region. A significant relationship (chi2 = 9.58, P < 0.01) in cestode co-infection with M. avium was found. There was no significant (chi2 = 1.66, P > 0.05) relationship in nematode co-infection with M. avium.


Subject(s)
Chickens , Helminthiasis, Animal/immunology , Mycobacterium avium/immunology , Poultry Diseases/immunology , T-Lymphocytes/immunology , Tuberculin/immunology , Tuberculosis, Avian/immunology , Altitude , Animals , Comorbidity , Cross-Sectional Studies , Ethiopia/epidemiology , Factor Analysis, Statistical , Gastrointestinal Diseases/epidemiology , Gastrointestinal Diseases/parasitology , Helminthiasis, Animal/epidemiology , Helminthiasis, Animal/parasitology , Lymphocyte Activation , Poultry Diseases/blood , Surveys and Questionnaires , Tuberculosis, Avian/epidemiology
6.
Neurology ; 59(3): 455-7, 2002 Aug 13.
Article in English | MEDLINE | ID: mdl-12177387

ABSTRACT

Clinical, biochemical, and genetic features of a Spanish family with mitochondrial neurogastrointestinal encephalomyopathy are reported. The proband presented with severe gastrointestinal dysmotility and the affected sister had extraocular muscle weakness. In both affected individuals, biochemical defects of thymidine phosphorylase and a pathogenic G-to-A transition mutation at nucleotide 435 in the thymidine phosphorylase gene were identified. The first thymidine phosphorylase mutation identified in Spain showed phenotypic variability at onset.


Subject(s)
Genetic Variation/genetics , Mitochondrial Encephalomyopathies/genetics , Thymidine Phosphorylase/genetics , Adult , Female , Humans , Male , Mitochondrial Encephalomyopathies/enzymology , Mitochondrial Encephalomyopathies/physiopathology , Pedigree , Phenotype , Spain
7.
Semin Cell Dev Biol ; 12(6): 417-27, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11735376

ABSTRACT

Depletion and multiple deletions of mitochondrial DNA (mtDNA) have been associated with a growing number of autosomal diseases that have been classified as defects of intergenomic communication. MNGIE, an autosomal recessive disorder associated with mtDNA alterations is due to mutations in thymidine phosphorylase that may cause imbalance of the mitochondrial nucleotide pool. Subsequently, mutations in the mitochondrial proteins adenine nucleotide translocator 1, Twinkle, and polymerase gamma have been found to cause autosomal dominant progressive external ophthalmoplegia with multiple deletions of mtDNA. Uncovering the molecular bases of intergenomic communication defects will enhance our understanding of the mechanisms responsible for maintaining mtDNA integrity.


Subject(s)
Chromosome Disorders/genetics , DNA, Mitochondrial/genetics , Gene Deletion , Humans , Mitochondrial Diseases/genetics , Mitochondrial Diseases/metabolism , Mitochondrial Encephalomyopathies/genetics , Models, Genetic
8.
Graefes Arch Clin Exp Ophthalmol ; 239(6): 437-40, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11561792

ABSTRACT

BACKGROUND: in Leber's hereditary optic neuropathy, increased optic nerve cupping has been reported by several authors. Recently, a mitochondrial DNA (mtDNA) mutation at nucleotide 11778 typically associated with Leber's hereditary optic neuropathy (LHON) was identified in a patient treated for glaucoma but lacking typical signs of LHON. The question arises: should all normal-tension glaucoma patients be further evaluated for LHON? METHODS: we screened 54 unselected patients with normal-tension glaucoma (age range 20-96 years, 16 men and 38 women) for the primary mtDNA LHON mutations at nucleotides 3460, 11778 and 14484. RESULTS: none of the patients harboured the mtDNA mutations at nucleotides 3460, 11778 or 14484 (95% confidence intervals for each mutation ranged from 0% to 5.3%). CONCLUSIONS: primary LHON mtDNA mutations are rare or absent in unselected normal-tension glaucoma patients. Therefore, unselected normal-tension glaucoma patients should not be screened for these mutations. It is probable that only normal-tension glaucoma patients with atypical features (rapid progression, early deep central scotoma, pallor of neuroretinal rim, elevated disc, peripapillary teleangiectasia) or a positive family history of visual loss compatible with a matrilinear transmission should be further evaluated.


Subject(s)
DNA, Mitochondrial/genetics , Glaucoma, Open-Angle/genetics , Optic Atrophy, Hereditary, Leber/genetics , Adult , Aged , Aged, 80 and over , DNA Mutational Analysis , DNA Primers/chemistry , Female , Humans , Intraocular Pressure , Male , Middle Aged , Polymerase Chain Reaction
9.
J Biol Chem ; 275(43): 33373-8, 2000 Oct 27.
Article in English | MEDLINE | ID: mdl-10938275

ABSTRACT

We report the discovery of a class of pyrazole-based compounds that are potent inhibitors of the dihydroorotate dehydrogenase of Helicobacter pylori but that do not inhibit the cognate enzymes from Gram-positive bacteria or humans. In culture these compounds inhibit the growth of H. pylori selectively, showing no effect on other Gram-negative or Gram-positive bacteria or human cell lines. These compounds represent the first examples of H. pylori-specific antibacterial agents. Cellular activity within this structural class appears to be due to dihydroorotate dehydrogenase inhibition. Minor structural changes that abrogate in vitro inhibition of the enzyme likewise eliminate cellular activity. Furthermore, the minimum inhibitory concentrations of these compounds increase upon addition of orotate to the culture medium in a concentration-dependent manner, consistent with dihydroorotate dehydrogenase inhibition as the mechanism of cellular inhibition. The data presented here suggest that targeted inhibition of de novo pyrimidine biosynthesis may be a valuable mechanism for the development of antimicrobial agents selective for H. pylori.


Subject(s)
Anti-Bacterial Agents/pharmacology , Enzyme Inhibitors/pharmacology , Helicobacter pylori/drug effects , Oxidoreductases Acting on CH-CH Group Donors , Oxidoreductases/antagonists & inhibitors , Pyrimidines/biosynthesis , Amino Acid Sequence , Dihydroorotate Dehydrogenase , Dose-Response Relationship, Drug , Helicobacter pylori/enzymology , Kinetics , Molecular Sequence Data , Oxidoreductases/chemistry , Ubiquinone/chemistry , Ubiquinone/metabolism
10.
Ann Neurol ; 47(6): 792-800, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10852545

ABSTRACT

Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an autosomal recessive disorder defined clinically by severe gastrointestinal dysmotility; cachexia; ptosis, ophthalmoparesis, or both; peripheral neuropathy; leukoencephalopathy; and mitochondrial abnormalities. The disease is caused by mutations in the thymidine phosphorylase (TP) gene. TP protein catalyzes phosphorolysis of thymidine to thymine and deoxyribose 1-phosphate. We identified 21 probands (35 patients) who fulfilled our clinical criteria for MNGIE. MNGIE has clinically homogeneous features but varies in age at onset and rate of progression. Gastrointestinal dysmotility is the most prominent manifestation, with recurrent diarrhea, borborygmi, and intestinal pseudo-obstruction. Patients usually die in early adulthood (mean, 37.6 years; range, 26-58 years). Cerebral leukodystrophy is characteristic. Mitochondrial DNA (mtDNA) has depletion, multiple deletions, or both. We have identified 16 TP mutations. Homozygous or compound heterozygous mutations were present in all patients tested. Leukocyte TP activity was reduced drastically in all patients tested, 0.009 +/- 0.021 micromol/hr/mg (mean +/- SD; n = 16), compared with controls, 0.67 +/- 0.21 micromol/hr/mg (n = 19). MNGIE is a recognizable clinical syndrome caused by mutations in thymidine phosphorylase. Severe reduction of TP activity in leukocytes is diagnostic. Altered mitochondrial nucleoside and nucleotide pools may impair mtDNA replication, repair, or both.


Subject(s)
Gastrointestinal Diseases/genetics , Intestinal Pseudo-Obstruction/genetics , Mitochondrial Encephalomyopathies/genetics , Mutation , Thymidine Phosphorylase/genetics , Adult , Age of Onset , Blepharoptosis , Ethnicity , Exons , Female , Genes, Recessive , Humans , Introns , Male , Middle Aged , Mitochondria, Muscle/metabolism , Muscle, Skeletal/pathology , Nuclear Family , Open Reading Frames , Ophthalmoplegia , Point Mutation , Sequence Deletion , Syndrome
11.
Scand J Immunol ; 51(2): 147-54, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10652161

ABSTRACT

The aim of the present study was to assess the compartmentalized immune response, in terms of cytokine secretion and cell activation, in lungs and spleens of mice with slowly progressive primary tuberculosis. Immunocyte populations from both organs were isolated and stimulated with concanavalin A, purified protein derivatives and MPT 59. Production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was measured using an enzyme-linked immunosorbent assay, and cell activation was measured using a tetrazolium colorimetric assay. The IFN-gamma and IL-4 levels in the supernatants of Mycobacterium tuberculosis antigen (Ag)-stimulated lung immunocytes from infected mice were higher than the levels from uninfected mice. However, only IL-4 levels were raised in the supernatants of Ag-stimulated spleen immunocytes from infected mice. Spontaneous and Ag-stimulated immunocyte activation was lower only in the lungs of infected mice compared with uninfected mice. The level of lung immunocyte activation was inversely associated with the extent of gross pulmonary pathology. In conclusion, cytokine secretion and cell activation were different between lungs and spleens in slowly progressive primary murine tuberculosis. Cytokine diversity may explain the confinement of tuberculous lesions in the lungs and the absence of lesions in the spleens of mice with slowly progressive tuberculosis.


Subject(s)
Lung/immunology , Spleen/immunology , Tuberculosis, Pulmonary/immunology , Animals , Cells, Cultured , Culture Media , Disease Progression , Female , Interferon-gamma/biosynthesis , Interleukin-4/biosynthesis , Lung/cytology , Lung/pathology , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Mycobacterium tuberculosis/immunology , Spleen/cytology , Spleen/pathology , Stem Cells
SELECTION OF CITATIONS
SEARCH DETAIL
...