ABSTRACT
BACKGROUND: Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reactions. Therefore, a method to measure antibodies, including IgG, is necessary. We investigated ABO antibody titers of healthy individuals using a column agglutination technique (CAT) with or without dithiothreitol (DTT) and compared them with titers obtained using a conventional tube method. METHODS: Among healthy adults who underwent a medical examination, 180 individuals (60 with blood group A, 60 with group B, and 60 with group O) were selected. Antibody titrations were performed using the immediate spin (IS) tube, anti-human globulin (AHG) tube, and CAT with or without DTT methods. RESULTS: Higher median values of anti-B and anti-A titers in groups A and B individuals, respectively, were obtained using the IS method than using the AHG method. Higher values for group O individuals were obtained using the AHG method. Higher median titers of anti-B and anti-A in group O individuals were obtained using CAT without DTT than using the AHG method. Median titers of anti-B and anti-A in all blood groups were higher in CAT without DTT than in CAT with DTT, especially for group O individuals. CONCLUSIONS: We recommend CAT with and without DTT for titration of anti-A and anti-B, especially in group O individuals, to provide more sensitive results that include IgG data. Adjustment of insurance coverage of fees associated with antibody titration might be necessary, considering the actual cost of reagents and personnel.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , ABO Blood-Group System/immunology , Agglutination Tests/instrumentation , Antibodies/analysis , Immunoglobulin G/immunologyABSTRACT
Two trials of external quality assessment for conventional newborn screening tests for phenylketonuria, congenital hypothyroidism, galactosemia, maple syrup urine disease and homocytinuria and for the newborn screening tests using tandem mass spectrometry (MS/MS) were performed in 2009. Total 20 specimens were distributed to 13 laboratories. All the control materials were send as dried blood spots. The response rate was 100%. The mean, SD, CV, median and range were analyzed. In addition, two trials of external quality assessment for glycohemoglobin (HbA1c) were performed in 2009. Total 6 samples were distributed to 196 laboratories and the response rate was 97.2% (175/180) and 98.5% (193/196) in each trial. From this trial, we performed accuracy-based survey which used challenge specimens that were free from matrix effects and had target values traceable to certified reference material. Bias between each participants result and reference target value were evaluated.
Subject(s)
Humans , Infant, Newborn , Bias , Congenital Hypothyroidism , Galactosemias , Korea , Maple Syrup Urine Disease , Mass Screening , Phenylketonurias , Tandem Mass SpectrometryABSTRACT
Two trials of external quality assessment for the newborn screening tests of inborn errors of metabolism including PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen and homocytinuria screen and newborn screening tests using tandem mass spectrometry were performed in 2008. Total 20 specimens for the newborn screening tests were distributed to 14 laboratories. The response rate was 100%. All the control materials were sent as dried blood spot forms. The mean, SD, CV, median and range were analyzed. The newborn screening tests using tandem mass spectrometry (MS/MS) were analyzed that the participants results for amino acids, acylcarnitine and their cutoff values. In addition, two trial of external quality assessment for glycohemoglobin (HbA1c) was performed in 2008. Total five samples were distributed to 238 laboratories and the response rate was 95.8% (228/238) and 97.4% (225/231), in each. Statistical data such as mean, SD and CV were provided for peer groups.
Subject(s)
Humans , Infant, Newborn , Amino Acids , Carnitine , Galactosemias , Korea , Maple Syrup Urine Disease , Mass Screening , Metabolism, Inborn Errors , Peer Group , Tandem Mass SpectrometryABSTRACT
Two trials of external quality assessment for the newborn screening tests of inborn errors of metabolism including PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen and homocytinuria screen, were performed in 2007. Total 20 specimens for the newborn screening tests were distributed to 16 laboratories. The response rate was 100%. All the control materials were sent as filter paper forms. The mean, SD, CV, median and range were analyzed. From this trial, the proficiency tests for newborn screening tests using tandem mass spectrometry (MS/MS) were included as routine quality assessment. Participants results for amino acids, acylcarnitine and their cutoff values were analyzed. In addition, the trial of external quality assessment for glycohemoglobin (HbA1c) was first started in 2007. Two samples prepared from normal whole blood and commercially available abnormal control materials, were distributed to 246 laboratories and the response rate was 93%. Statistical data such as mean, SD and CV were provided for peer groups.
Subject(s)
Humans , Infant, Newborn , Amino Acids , Carnitine , Galactosemias , Korea , Maple Syrup Urine Disease , Mass Screening , Metabolism, Inborn Errors , Peer Group , Tandem Mass SpectrometryABSTRACT
Two trials of external quality assessment for the newborn screening tests of inborn errors of metabolism including PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen and homocytinuria screen, were performed in 2007. Total 20 specimens for the newborn screening tests were distributed to 16 laboratories. The response rate was 100%. All the control materials were sent as filter paper forms. The mean, SD, CV, median and range were analyzed. From this trial, the proficiency tests for newborn screening tests using tandem mass spectrometry (MS/MS) were included as routine quality assessment. Participants results for amino acids, acylcarnitine and their cutoff values were analyzed. In addition, the trial of external quality assessment for glycohemoglobin (HbA1c) was first started in 2007. Two samples prepared from normal whole blood and commercially available abnormal control materials, were distributed to 246 laboratories and the response rate was 93%. Statistical data such as mean, SD and CV were provided for peer groups.
Subject(s)
Humans , Infant, Newborn , Amino Acids , Carnitine , Galactosemias , Korea , Maple Syrup Urine Disease , Mass Screening , Metabolism, Inborn Errors , Peer Group , Tandem Mass SpectrometryABSTRACT
The trial of external quality assessment for inborn error of metabolism was performed in 2005. Total 11 specimens for the conventional newborn screening tests were distributed to 47 laboratories. The response rates were 92.5% (37/40) and 100% (47/47), in each trial. All the control materials were sent as filter paper forms. Each laboratory replied the test results of the screening items that they were doing as a routine test at the reception of the specimen among PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen, homocystinuria screen and histidinemia screen. The mean, SD, CV, median and range were analyzed. The pilot proficiency test for newborn screening using tandem mass spectrometry (MS/MS) was also performed. Total 8 blood spots were distributed to 9 laboratories performing newborn screening using MS/MS. Participants' results for amino acids & acylcarnitine and their cutoff values were analyzed.
Subject(s)
Humans , Infant, Newborn , Amino Acids , Galactosemias , Homocystinuria , Korea , Maple Syrup Urine Disease , Mass Screening , Metabolism , Tandem Mass SpectrometryABSTRACT
The trial of external quality assessment for inborn error of metabolism was performed twice in 2004. Total 10 specimens for the conventional newborn screening tests were distributed to 43 laboratories. The response rates were 90.7%(39/43) in first trial and 90%(36/40) in second trial. All the control materials were sent as filter paper forms. Each laboratory replied the test results as the screening items they were doing as a routine test at the reception of the specimen among PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen, homocytinuria screen and histidinemia screen. The mean, SD, CV, median and range were analyzed. From this trial, pilot proficiency test for newborn screening using tandem mass spectrometry (MS/MS) was also performed. Total 8 blood spots were distributed to 7 laboratories performing newborn screening using MS/MS. Participants results for amino acids & acylcarnitine and their cutoff values were analyzed.
Subject(s)
Humans , Infant, Newborn , Amino Acids , Galactosemias , Korea , Maple Syrup Urine Disease , Mass Screening , Metabolism , Tandem Mass SpectrometryABSTRACT
BACKGROUND: There have been many efforts to determine maximum surgical blood order schedule (MSBOS) of elective surgery in Korea since 1980?. But most of these data were confined to single hospital, so it was somewhat irrelevant to suggest them as general data in Korea. In this study, we tried to establish MSBOS which can be suggested as substantial and objective data in our country and to increase the efficiency of limited blood products. METHODS: From September 2003 to February 2004, we analyzed average RBC utilization for elective surgery in four university hospitals (Soonchunhyang University Hospital, Soonchunhyang University Bucheon Hospital, Korea University Ansan Hospital, Ajou University Hospital) by referring to anesthesiologic records and blood delivery sheets. Also, we established MSBOS range in operations performed more than five times in three among four hospitals. RESULTS: Sixty-seven operations were analyzed in our study and 18 of them were performed in department of general surgery. Type and screen (T&S) was recommended in 69.8~83.6% and the percentage of operations recommended as T&S in all four hospitals was 52.2%. Operations of obstetric/gynecology, urology and otorhinolaryngology were mostly applicable to T&S. CONCLUSION: We suggested comprehensive and substantial MSBOS range by analyzing the operations performed in four hospitals whose operation numbers ranged from 470 to 1,200 per month. It was considered that MSBOS range, which could be applicable to various hospitals, should be reestablished and enforced regularly to decrease medical cost and improve blood utility
Subject(s)
Appointments and Schedules , Hospitals, University , Korea , Otolaryngology , UrologyABSTRACT
BACKGROUND: Despite significant progress in vaccine and therapeutic regimen, hepatitis B virus (HBV) infection remains one of the major diseases. Long-term use of lamivudine can induce the emergence of drug resistance. TRUGENE(TM) HBV Genotyping (Visible Genetics Inc., Ga, USA) is an assay that reports the viral genotype and mutations likely to confer resistance to antiviral therapy. In this study, we analyzed HBV genotype and mutations and correlated them with the histologic grade and stage of the liver disease to provide the useful information about the therapy of chronic liver disease. METHODS: HBV DNA was isolated from 86 patients with HBV-associated chronic liver diseases and analyzed by TRUGENE(TM) HBV Genotyping. Histologic grade and stage were correlated with RESULTS: HBV genotypes of 86 patients were all C (100%). Mutations associated with lamivudine resistance were detected in 10 patients (11.6%) and M204I (YIDD) mutant was the most common. Unknown mutation such as L180F was also detected. Statistical analysis showed that the number of coding changes at HBsAg region was significantly correlated with the lobular activity (P=0.01). CONCLUSIONS: All patients were genotype C and lamivudine resistant mutations were detected in 11.6%. L180F mutation, not known previously, was detected in one case. Number of coding changes at HBsAg region was significantly correlated with the lobular activity. It was considered that follow-up studies about the clinical significance of coding changes in HBsAg are needed, and that a further study such as in vitro transfection is necessary to confirm the possibility of a novel mutation of L180F.
Subject(s)
Humans , Clinical Coding , DNA , Drug Resistance , Genetics , Genotype , Hepatitis B Surface Antigens , Hepatitis B virus , Hepatitis , Lamivudine , Liver Diseases , TransfectionABSTRACT
BACKGROUND: Blood supply in Korea has been unstable in more than a year because transfusion-related infection was repeated in patients receiving blood that the Korean Red Cross Blood Center (KRCBC) had supplied. The purpose of this study is to contribute to stable and efficient blood supply in our country by analysis of present status of hospital blood banks as blood donation center and their satisfaction levels with the KRCBC. METHODS: From July to August 2004, we performed questionnaire survey in 129 hospital blood banks that the KRCBC issued donor card in 2003. Among them, 73 hospitals replied and we analyzed them. RESULTS: Fifty-one (69.8%) among 73 hospital blood banks collected less than 100 cases of blood donation in 2003 and 16 of them collected no blood component. Satisfaction level with KRCBC was only 1.8 in hospitals less than 300 beds. Improvement in the delivery of blood components and blood testing of donated blood were in highest need among all areas of services supplied by KRCBC. Hospitals more than 1,000 beds answered that they would not transfer the collection service to KRCBC no matter how NAT be performed in all donated blood because of the directed and autologous donation of their hospitals. CONCLUSION: Satisfaction level of Hospital blood banks in Korea with blood services of KRCBC was rated below average, especially in hospitals less than 300 beds. It is important that the government should be the subject of national blood services and suggest appropriate schemes such as national audit program through close cooperation with the KRCBC and hospitals.
Subject(s)
Humans , Blood Banks , Blood Donors , Hematologic Tests , Korea , Red Cross , Tissue and Organ Procurement , Surveys and QuestionnairesABSTRACT
The trial of external quality assessment for inborn error of metabolism was performed in 2003. A total 10 specimens for neonatal screening tests were distributed to 43 laboratories with a response rate of 83%. All the control materials were sent as a filter paper form. Each laboratory replied the test result as the screening items they were doing as a rountine test at the reception of the specimen among PKU screening, neonatal TSH, neonatal T4(total/free), galactosemia screen, homocytinuria screen and histidinemia screen. The mean, SD, and CV were analyzed.
Subject(s)
Infant, Newborn , Galactosemias , Korea , Mass Screening , Metabolism , Neonatal ScreeningABSTRACT
The trial of external quality assessment for inborn error of metabolism was performed in 2002. Total 10 specimens for neonatal screening tests were distributed to 61 laboratories with a response rate of 70.5%(43/61). All the control materials were sent as filter paper forms. Each laboratory replied the test results as the screening items they were testing routinely at the reception of the specimen among PKU screening, TSH, T4 (total/free), galactosemia screen, maple syrup urine disease screen, homocytinuria screen and histidinemia screen. The mean, SD, CV, median and range were analyzed.
