ABSTRACT
Abstract Biofilm formation by Bacillus cereus strains is now recognized as a systematic contaminaron mechanism in foods; the aim of this study was to evaluate the production of submerged and interface biofilms in strains of B. cereus group in different materials, the effect of dex-trose, motility, the presence of genes related to biofilms and the enterotoxigenic profile of the strains. We determine biofilm production by safranin assay, motility on semi-solid medium, toxin gene profiling and genes related to biofilm production by PCR in B. cereus group iso-lated from food. In this study, we observe strains used a higher production of biofilms in PVC; in the BHI broth, no submerged biofilms were found compared to phenol red broth and phenol red broth supplemented with dextrose; no strains with the ces gene were found, the enterotoxin profile was the most common the profile that includes genes for the three enterotoxins. We observed a different distribution of tasA and sipW with the origin of isolation of the strain, being more frequent in the strains isolated from eggshell. The production and type of biofilms are differential according to the type of material and culture medium used.
Resumen La formación de biopelículas por cepas de Bacillus cereus es reconocida como un mecanismo de contaminación sistemática en alimentos; el objetivo del estudio fue evaluar la producción de biopelículas sumergidas y de superficie en cepas del grupo de Bacillus cereus en diferentes materiales, el efecto de la dextrosa, la motilidad, la presencia de genes relacionados a biopelículas y el perfil enterotoxigénico de las cepas. Determinamos la producción de biopelículas por el ensayo de safranina, motilidad en medio sólido, perfil enterotoxigénico y genes relacionados a producción de biopelículas por PCR en aislados del grupo de Bacillus cereus de alimentos. En este estudio, observamos en las cepas utilizadas una alta producción de biopelículas en PVC; en caldo BHI, no se encontraron biopelículas sumergidas en comparación con el caldo rojo de fenol y caldo rojo de fenol suplementando con dextrosa; no se encontraron cepas con el gen ces, el perfil de enterotoxinas más común fue el perfil que incluía los genes de las tres enterotoxinas, también observamos una distribución diferente de tasA y sipW con relación al origen de la cepa, siendo más frecuente estos genes en las cepas aisladas de huevos. La producción y el tipo de biopelículas es diferente de acuerdo con el tipo de material y el medio de cultivo utilizado.
ABSTRACT
Biofilm formation by Bacillus cereus strains is now recognized as a systematic contamination mechanism in foods; the aim of this study was to evaluate the production of submerged and interface biofilms in strains of B. cereus group in different materials, the effect of dextrose, motility, the presence of genes related to biofilms and the enterotoxigenic profile of the strains. We determine biofilm production by safranin assay, motility on semi-solid medium, toxin gene profiling and genes related to biofilm production by PCR in B. cereus group isolated from food. In this study, we observe strains used a higher production of biofilms in PVC; in the BHI broth, no submerged biofilms were found compared to phenol red broth and phenol red broth supplemented with dextrose; no strains with the ces gene were found, the enterotoxin profile was the most common the profile that includes genes for the three enterotoxins. We observed a different distribution of tasA and sipW with the origin of isolation of the strain, being more frequent in the strains isolated from eggshell. The production and type of biofilms are differential according to the type of material and culture medium used.
Subject(s)
Bacillus , Bacillus cereus/genetics , Phenolsulfonphthalein/analysis , Enterotoxins/genetics , Enterotoxins/analysis , Food Microbiology , Biofilms , GlucoseABSTRACT
Ex-situ conservation in hatcheries is a successful strategy for the recovery of sea turtle populations. However, it alters the ontogenesis of the brain and gonads, as well as body size and locomotor performance at nest emergence. Relocation to hatcheries may alter immune system development, since this depends highly on the nest environment. We hypothesized that ex-situ brooding would negatively associate with immune traits of Lepidochelys olivacea. Splenic cytoarchitecture and leukocyte quantification were used as proxies for the immune configuration. Body size, gonadal sex and sand temperature during incubation were determined. Additionally, the success of nest hatching and emergence was quantified. Linear mixed models of splenic cytoarchitecture, leucocyte proportions and body size, using sex and nest type as explanatory variables, evaluated the effects of ex-situ brooding. Generalized linear mixed models using quasibinomial distributions (log link) analyzed effects on hatching and emergence success. Hatchlings from ex-situ nests were heavier, larger and showed a greater spleen-somatic index. They showed more and better defined splenic periarteriolar lymphoid sheaths, as well as a higher proportion of heterophils but less monocytes. Moreover, ex-situ brooding increased hatching and emergence success. Sand temperatures in hatcheries favored male sex determination, while the opposite occurred for in-situ incubation. Interestingly, the immune configuration and body size were independent of sex but associated with ex-situ conservation. Greater body size promotes early hatchling survival, while better spleen development is related to a greater antibody production and a better immune response to pathogens. Altogether, the results suggest that ex-situ incubation is associated with a better immune configuration and higher survival success.
