ABSTRACT
The mutagenicity of interstitial water and organic extracts from the sediments in the Cadeia and Feitoria Rivers, RS, Brazil, were evaluated by Salmonella microsuspension bioassay using TA97a, TA98, TA100 and TA102 strains, in the absence and presence of S9 mix. At the contaminated site, the mutagenic responses for interstitial water, suggested the presence of frameshift and base pair substitution mutagens, including oxidative substances. Organic extracts presented direct or indicative mutagenesis to the TA97a, TA98 and TA100 strains. In general, an exogenous metabolic systems decreased the mutagenicity of the samples. High concentrations of total chromium found in the sediment and interstitial water as well as total mercury in the sediment of the contaminated site, when compared to the control area, may help explain the mutagenic results. The livers of Gymnogeophagus gymnogenys collected in this impacted area, compared to a non-polluted site, were analyzed for oxidative stress parameters. Compared to the controls, there was a significant increase in the activity of superoxide dismutase (SOD) at levels of substances reactive to thiobarbituric acid (TBARS), and in the chemiluminescence of hepatic cells in fish in the polluted area. The concentration of cytochromes P450 and b5 decreased drastically in the fish at the polluted site, while the catalase activity did not change. It was possible to correlate the biological changes in the fish with the presence of mutagenic compounds in sediment and interstitial water in this area.
Subject(s)
Cichlids/metabolism , Geologic Sediments/analysis , Oxidative Stress/drug effects , Rivers/chemistry , Water Pollutants, Chemical/toxicity , Animals , Biological Assay , Brazil , Catalase/metabolism , Cytochrome P-450 Enzyme System/metabolism , Enzyme Induction/drug effects , Lipid Peroxidation/drug effects , Liver/metabolism , Luminescent Measurements , Metals, Heavy/analysis , Mutagenicity Tests , Salmonella/genetics , Superoxide Dismutase/biosynthesis , Tanning , Thiobarbituric Acid Reactive Substances/metabolism , Water Pollutants, Chemical/analysisABSTRACT
As infecções urinárias são freqüentes nos rebanhos suínos, sendo a principal causa de descarte e mortalidade de animais adultos. Apesar das características multifatoriais da doença o microrganismo freqüentemente isolado é a Escherichia coli. Vários fatores de virulência de Escherichia coli foram descritos em amostras uropatogênicas e permitem diferenciar cepas patogênicas de não patogênicas. Esta revisão tem por objetivo apresentar alguns tópicos relativos aos fatores de virulência presentes em amostras de E. coli uropatogênicas para suínos.
Subject(s)
Bacteria , Swine/microbiologyABSTRACT
The livers fractions of Oreochromis niloticus (Tilapia) and Wistar rats taken from treated animals to single intraperitoneal doses of hexavalent chromium (K(2)Cr(2)O(7)), were analyzed for tert butyl hydroperoxide-initiated chemiluminescence (CL), lipid peroxidation using thiobarbituric acid reactive substances (TBARS), enzymes superoxide dismutase (SOD) and catalase activities, and the quantification of cytochromes P450 and b5. The CL time course curve was significantly higher in O. niloticus treated with Cr(VI) at all times studied. The maximum CL was observed after 24h of exposure. The CL mean ratio treated/control was 4.6 and the initial velocity (V(0)) increased 7.4 times at 24h of intoxication. The TBARS levels however increased only 24h after intoxication. The CL time course curve was significantly higher in rats treated with Cr(VI) as early as 3h after intoxication. The maximum CL occurred 24h after exposure. The CL mean ratio treated/control was 2.1 and the V(0) increased 3.8 times at 24h of intoxication. On the contrary, was not observed any increase in TBARS in this study. Compared to the controls, in fish, SOD activity increased significantly only 24h after of exposure. In rats, there was a significant increase in SOD activity after 3 and 24h of intoxication. There was no catalase activity, nor cytochrome P450 and cytochrome b5 variation in both species studied. Through CL approach, it was possible to detect oxidative stress as early as 15min in fish and 3h in rats. Also a marked oxidative stress was revealed by the increased CL parameters that at 24h of intoxication was accompanied by arose SOD activity in liver of O. niloticus and Wistar rats and increased TBARS in O. niloticus. In addition, it was possible to show higher levels of oxidative stress in fish compared to the rat in spite of the dose to be four times smaller. Furthermore, CL provide a sensitive method for possible use to detect earlier biological impact in contaminated environments.
ABSTRACT
Com o objetivo de identificar a patogenicidade e resistência a antimicrobianos da cepa de E. coli BK99, foram utilizados alguns testes: aglutinaçäo em lâmina para detecçäo da fímbria F5, produçäo de STa, ensaios para hemolisinas e colicinas, patogenicidade em leitöes e antibiograma. A cepa BK99 apresentou o seguinte perfil: F1(+), F5(+), STa(+), Col V(+), Hly(-), ST(R), KA(R), NO(R), TT(R) SF(R) e foi capaz de provocar a doença clínica e morte em leitöes inoculados; também foi possível o resgate dessa cepa de fezes diarréicas e do conteúdo intestinal dos leitöes revelando, assim, alto índice de recuperaçäo de colônias portadoras da fímbria F5(+). Os resultados permitem concluir que a cepa de E. coli BK99 é produtora de fatores de virulência e reproduz experimentalmente a colibacilose suína neonatal.
Subject(s)
Animals , Diarrhea/veterinary , Swine Diseases/virology , Escherichia coli , Escherichia coli Infections/veterinary , Escherichia coli Infections/virology , Swine , VirulenceABSTRACT
A Escherichia coli é o principal agente infeccioso envolvido nas diarréias dos leitöes lactentes, sendo usada terapia através de drogas para controlar esta doença. Foram estudadas 224 cepas de Escherichia coli isoladas de leitöes lactentes com diarréia em relaçäo à sensibilidade antimicrobiana. Os resultados mostram alta resistência a três antimicrobianos na inibiçäo do crescimento de cepas de Escherichia coli, isoladas de leitöes lactentes, com diarréia, no sudoeste do Paraná.
Subject(s)
Animals , Anti-Bacterial Agents/pharmacology , Swine Diseases/drug therapy , Escherichia coli , Diarrhea/drug therapy , Diarrhea/veterinary , Drug Resistance, Microbial , Swine/microbiologyABSTRACT
Considerando a interaçäo complexa entre diferentes compostos químicos e metais, a influência desses fenômenos sobre o meio ambiente e consequentemente, sobre o ser humano, esta revisäo tem como objetivo mostrar que o Teste de Ames ainda hoje, é uma ferramenta importante na detecçäo de mutágenos
