Impacts of chitosan and its nanoformulations on the metabolic syndromes: a review.

A significant public health issue worldwide is metabolic syndrome, a cluster of metabolic illnesses that comprises insulin resistance, obesity, dyslipidemia, hyperglycemia, and hypertension. The creation of natural treatments and preventions for metabolic syndrome is crucial. Chitosan, along with its nanoformulations, is an oligomer of chitin, the second-most prevalent polymer in nature, which is created via deacetylation. Due to its plentiful biological actions in recent years, chitosan and its nanoformulations have drawn much interest. Recently, the chitosan nanoparticle-based delivery of CRISPR-Cas9 has been applied in treating metabolic syndromes. The benefits of chitosan and its nanoformulations on insulin resistance, obesity, diabetes mellitus, dyslipidemia, hyperglycemia, and hypertension will be outlined in the present review, highlighting potential mechanisms for the avoidance and medication of the metabolic syndromes by chitosan and its nanoformulations.

The interplay between vitamin D and COVID-19: protective or bystander?

The world is currently facing the COVID-19 pandemic, caused by the novel Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Due to a lack of specific treatment and prophylaxis, protective health measures that can reduce infection severity and COVID-19 mortality are urgently required. Clinical and epidemiological studies have shown that vitamin D deficiency can be linked to an increased risk of viral infection, including COVID-19. Therefore, in this review, we looked at various possible roles of vitamin D in reducing the risk of COVID-19 infection and severity. We describe in this article that individuals at high risk of vitamin D deficiency should consider taking vitamin D supplements to keep optimal concentrations. Moreover, we discuss different possible mechanisms by which vitamin D can efficiently reduce the risk of infections through modulation of innate and adaptive immunity against various types of infections. It is advisable to perform further studies addressing the observed influence of vitamin D levels to reduce the risk of COVID-19 infection and mortality.

Growth, carcass characteristics, meat quality, and microbial aspects of growing quail fed diets enriched with two different types of probiotics (Bacillus toyonensis and Bifidobacterium bifidum).

The present investigation aimed to explore the impact of dietary graded levels of 2 types of probiotic bacteria (Bacillus toyonensis [BT] and Bifidobacterium bifidum [BB]) on growth, carcass traits, meat quality, and bacteriology of growing Japanese quail reared under the cage system. One thousand three hundred sixty Japanese quail day-old chicks were randomly divided into 10 groups (8 replicates each). Birds were fed a basal diet (control, T1) and the basal diet plus 0.05, 0.075, 0.10, and 0.125% BT (T2, T3, T4, and T5, respectively), 0.10% BB (T6), and the same previous doses of BT plus 0.05% BB (T7, T8, T9, and T10, respectively). Results showed a significant (P < 0.001) increase in final BW and weight gain because of probiotic supplementation (except T2 for weight gain). Both feed intake and feed conversion ratio did not differ during the overall experimental period (1-42 D of age) except feed intake that was reduced in T2 and increased in T5 and T9 groups. All carcass traits studied were significantly (P < 0.01) affected by probiotics, and the combination between BT and BB in group T8 increased all studied parameters as compared with the other treatment groups. The quail meat color of redness a∗ and L∗ values, thiobarbituric content, cooking loss, proteolysis, and total coliform were decreased (P < 0.001) by probiotic treatment. In general, supplementing BT, BB, or their combination to the basal diet delayed the proliferation of pathogenic bacteria in the diet and intestine. Using BT and BB as feed supplements enhanced growth performance and meat quality of quails as well as diminished pathogenic bacteria proliferation in their diet and intestine. As per our results, we can recommend the application of T5 and T8 to T10 levels for the best performance, carcass traits, and meat quality of growing quails.

Effect of dietary supplementation of garlic powder and phenyl acetic acid on productive performance, blood haematology, immunity and antioxidant status of broiler chickens.

OBJECTIVE: The effect of garlic powder (GP) and phenyl acetic (PA) acid throughout the fattening period of broiler chickens on performance, blood parameters, immune, and antioxidant parameters as well as carcass traits was evaluated. METHODS: A total of 210 day-old Cobb broiler chicks were randomly distributed into seven dietary treatments having five replications with six chicks per replicate. The first group (control) fed a basal diet without supplements, whereas the 2nd, 3rd, and 4th group were fed basal diet plus 0.25, 0.50, and 0.75 g GP/kg diet, respectively and the group 5th, 6th, and 7th were fed on the basal diet plus 0.25, 0.50, and 0.75 g PA/kg diet. RESULTS: Broiler body weight and gain at 21 and 42 days were increased (p<0.05) with diets supplemented with GP and PA. Red blood cells and hemoglobin were improved in chickens fed diets enriched with GP. Broiler chickens received diets containing either GP or PA recorded the higher values (p<0.05) of total protein, globulin, high-density lipoprotein, immunoglobulin M (IgM), and IgG, superoxide dismutase and total antioxidant capacity; while, blood total cholesterol, low-density lipoprotein, aspartate-aminotransferase, and malondialdehyde were lowered (p<0.05) compared to control-diet. Liver and immune-related organs weight were improved (p<0.05) in broilers fed diet supplemented with GP and PA. CONCLUSION: Feeding of GP or PA in diet had positive effects on performance traits and immunological, antioxidant and physiological status of broilers. Thus, the use of tested feed additives as an eco-friendly alternative to antibiotics produced a positive effect on animal health.

Does the use of different oil sources in quail diets impact their productive and reproductive performance, egg quality, and blood constituents?

The present study investigated the impact of dietary oil sources (soybean, corn, peanut, flaxseed, olive, and sunflower oils as sources of omega 3, 6, and 9 fatty acids) on productive and reproductive traits, egg quality, hematological and biochemical blood parameters of laying Japanese quail. A total of 360 eight-week mature Japanese quail were randomly divided into 6 groups fed for 8 wk on a basal diet included with 1.5% of different oil sources. Results showed that the highest egg weights and the best feed conversion ratio (P < 0.01) were recorded for quail fed diets supplemented with 1.5% soybean and peanut oil. The highest hatchability percentages (P < 0.05) were recorded for quail fed diets supplemented with corn oil as compared to the other oils. Furthermore, diets enriched with corn, olive, or sunflower oils had higher values of blood lymphocytes (%) compared to the other treated groups. Blood total cholesterol significantly decreased in quail fed on corn, peanut, flaxseed, or olive oil sources as compared to soybean or sunflower oil groups. Immunologically, the highest levels (P < 0.001) of immunoglobulins (G and M) were recorded for quail fed on corn or olive oil sources compared to other oil sources. Quail consuming olive oil-included diets showed a significant increase in superoxide dismutase and glutathione S-transferase activities and a significant decrease on malondialdehyde level compared with those consumed the other oil sources. It could be concluded that varying the oil source can affect productive, reproductive, and health aspects of Japanese quail. Soybean oil showed good results regarding production aspects; however, olive oil was the best regarding health aspects.

Growth, carcass characteristics, and meat quality of broilers fed a low-energy diet supplemented with a multienzyme preparation.

The effect of a low-ME diet with a multienzyme (Kemzyme Plus, Kemin, Des Moines, IA) blend on performance, meat quality, and carcass traits was evaluated in Hubbard broiler chicks. A total of 120 Hubbard broiler chicks were allocated to the following 4 experimental groups and every group was separated into 6 replicates, with 5 birds per replicate: control (3,180 kcal/kg of ME), control + 0.50 g/kg diet of enzyme (Cont-Enz), low-ME diet (3,080 kcal/kg), and low-ME + 0.50 g/kg diet of enzyme (low-ME-Enz). The trail lasted for 16 D (32 to 48 D of age). No significant differences in growth parameters or carcass traits were observed among treatments. However, liver weight increased with the low-ME-Enz diet (P = 0.038). The low-ME diet recorded the highest weight for the bursa (P = 0.043) and thymus (P = 0.019). Dietary treatments had significant impacts on the length of duodenum, ileum, and cecum, as well as the weight of duodenum. The length of duodenum, ileum, and cecum increased with enzyme supplementation. The myofibril fragmentation index was lower with the Cont-Enz, low-ME, and low-ME-Enz diets than with the control diet (P = 0.043). The shear force increased with the low-ME-Enz diet (P = 0.022) than the control diet. Dietary treatments influenced breast meat yellowness (P = 0.019), whereas the low-ME diet had the lowest yellowness at the slaughtering age. The dietary treatments affected the breast meat pH (P = 0.001), with the control diet having the highest pH value after 24 hours. Thus, there was no effect of low-ME or enzyme supplementation to the control or low-ME diet on growth performance or carcass yield. However, feeding a low-ME diet or Cont-Enz preparation influenced organ and small intestine weights and meat characteristics.

Effects of stock, sex, and muscle type on carcass characteristics and meat quality attributes of parent broiler breeders and broiler chickens.

This study aimed to investigate the effect of using breeder parents (434-days-old) and broiler chickens (37 D of age) from Ross 308 on meat quality parameters and carcass characteristics; sex and muscle type were also estimated. Carcass assessment involved hot carcass weight, carcass yield, and cuts (breast, legs, wings, back, and shoulder). A total of 120 birds were divided into 2 groups (60 birds from each stock), each stock contain equal number of sexes (30 males and 30 females), and further divided into 6 replicates, so that there were 5 birds in each replicate. The birds were fed ad libitum. A sample of 30 birds (male, n = 15 and female, n = 15) from each group was slaughtered for assessing carcass and physical characteristics. Sensory analysis was carried out on each group after boiling or roasting the bird. Live weight, carcass weight and yield, breast weight, legs, wings, and back weights and yield were higher (P < 0.001) for breeder parents when compared to broilers, with the exception of breast percentage. Moreover, better carcass characteristics were observed in males than in females. Parent stock had better water-holding capacity and myofibrillar fragmentation whereas broilers had more tender meat than breeders. Moreover, cooking loss and other sensory characters were not significant between the two groups; however, females had more tender pectoral muscles and more myofibrillar fragmentation than males. We concluded that parent breeders had better carcass characteristics than broiler chickens. The broiler chickens of Ross 308 and female birds had better meat quality than parent breeders.

Report of ß-lactam antibiotic-induced vancomycin-resistant Staphylococcus aureus from a university hospital in Egypt.

We report a case of hospital-acquired pneumonia that to our knowledge is the first description in Egypt of the emergence of vancomycin (VA)-resistant Staphylococcus aureus due to the concomitant use of ß-lactams. The combination of ß-lactam antibiotics and VA in the treatment of methicillin-resistant S. aureus must be avoided to refrain from inducing VA resistance; further, if there is coinfection with Gram-negative bacilli, ß-lactams must be avoided. If ß-lactam antibiotic-induced VA-resistant methicillin-resistant S. aureus is isolated, then ß-lactams must be avoided until the organism's sensitivity to VA is restored if VA is the only therapeutic option available.

Impacts of various storage periods on egg quality, hatchability, post-hatching performance, and economic benefit analysis of two breeds of quail.

The effect of storage period on hatching and post-hatching performance of two quail breeds (brown Japanese quail (BJQ) and French white quail (FWQ)) was investigated using 940 eggs from each breed. Eggs were divided into four equal groups (235 eggs each), in each group. A total number of 210 eggs were used for incubation (with three replicates, 70 eggs each) and additional 25 eggs served as samples for egg quality parameters, each group was kept for special storage period. The first group was incubated on the same day of collection (zero day storage). Whereas the second, third, and fourth groups were stored for 4, 7, and 10 d, respectively. Increasing the storage period more than 4 d significantly decreased the relative albumen weight, yolk index, total hatchability, and fertile eggs but significantly increased the relative yolk/albumen ratio, absolute and relative egg weight loss. Moreover, FWQ eggs exhibited higher (P < 0.05) hatchability compared to BJQ eggs after 10 d of storage and yielded heavier chicks (P < 0.05) after all storage periods. The economic analysis indicated that the storage costs for FWQ eggs were significantly greater than those of BJQ at a 0 d of storage (2.42 vs. 4.81 US cent (¢); P < 0.05). Furthermore, the total costs for BJQ eggs were significantly lower than the total costs for FWQ eggs (3.0 vs. 7.0 ¢; P < 0.05). With respect to profitability, the total return represented by selling the chicks was calculated at 5.43 ¢ for BJQ and 9.01 ¢ for FWQ. The net return estimated for FWQ was significantly greater than that of BJQ (3.0 vs. 2.0 ¢; P < 0.05). However, the hatchability loss for FWQ was significantly greater than that of BJQ over different storage periods.