ABSTRACT
Unconjugated vitamin D and its metabolites were investigated in the pollen of Pinus nigra Ar. and Pinus sylvestris L. by TLC, HPLC and competitive radiochemical determination of 25-hydroxycholecalciferol (25-OHD3). It was found that vitamin D (D2, D3) was present in the pollen in amounts about 2 micrograms/10 g and 25-OHD3, 24,25-dihydroxycholecalciferol [24,25-(OH)2D3] and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] between 0.1 and 3 micrograms/10 g of pollen, dependent on pollen species and methods.
Subject(s)
Plants/analysis , Pollen/analysis , Steroids/analysis , Vitamin D/analysis , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Protein BindingSubject(s)
Calcifediol/blood , Adolescent , Adult , Female , Humans , Male , Middle Aged , Reference Values , SeasonsSubject(s)
Adrenal Hyperplasia, Congenital , Adrenal Hyperplasia, Congenital/genetics , Genetic Carrier Screening/methods , Steroid Hydroxylases/deficiency , 17-alpha-Hydroxyprogesterone , Adrenal Hyperplasia, Congenital/etiology , Female , HLA Antigens/analysis , Humans , Hydroxyprogesterones/blood , MaleABSTRACT
The concentration of testosterone was determined in sera of 122 HLA-typed women. Subsequently the women were classified in the category below or above the mean serum testosterone concentration. When the frequencies of HLA antigens were compared in these two categories of women, it was found that HLA-B5 and HLA-B12 antigens were significantly increased in the category of women with serum testosterone level above the mean concentration (P less than 0.01 and P less than 0.05, respectively). The frequency of HLA-B8 antigen was significantly decreased in the same category of women (P less than 0.05). The comparisons of the mean testosterone values of each HLA antigen and the variance analysis have also shown significant differences between the mean of HLA-B8 antigen and the means of other HLA antigens--HLA-A2, A3, A9, B5, B12 and Bw35. These results gave further conclusive evidence that gene(s) inside HLA region influence either the androgen hormone metabolism itself or cellular sensitivity to hormonal action as it has been presented for congenital adrenal hyperplasia.
Subject(s)
HLA Antigens/analysis , Testosterone/blood , Adolescent , Adult , Analysis of Variance , Female , Humans , PhenotypeABSTRACT
Endocrine studies in girls with precocious thelarche were compared with those of normal girls of similar ages. Girls with precocious thelarche showed breast development and oestrogenised vaginal smears as the only signs of precocious sexual development. A few of the girls were tall and some had advanced bone ages but these two findings were not consistently present in the same patient. Hormones--such as serum oestradiol, oestrone, delta 4-androstenedione, progesterone, dehydroepiandrosterone (DHEA), follicle-stimulating hormone, luteinising hormone, and prolactin, and urinary 17-ketosteroids--were measured. Only DHEA was different, being higher in girls with precocious thelarche. It is suggested that the high DHEA level may serve as a precursor for conversion to oestrogens in target tissues, breast, and vagina. This mechanism for oestrogenisation had been reported in other patients.
Subject(s)
Adrenal Gland Diseases/complications , Puberty, Precocious/etiology , 17-Ketosteroids/urine , Age Determination by Skeleton , Body Height , Child , Child, Preschool , Dehydroepiandrosterone/blood , Female , Gonadal Steroid Hormones/blood , Gonadotropins, Pituitary/blood , Humans , Infant , Puberty, Precocious/metabolismABSTRACT
The concentration of plasma testosterone (T) and dihydrotestosterone (DHT) was determined in 2 groups of nonpregnant and pregnant women. The 1st group consisted of normal women and the 2nd of women with recurrent pregnancy disorders of unknown etiology. Significantly higher concentration of plasma DHT in nonpregnant women from the 2nd group was found (44.9 +/- 22 ng/100 ml) as compared to nonpregnant normals (24.2 +/- 5.2 ng/100 ml), p less than 0.01. There was no difference in the concentration of plasma T between the groups studied (p = 0.165).
Subject(s)
Dihydrotestosterone/blood , Pregnancy Complications/blood , Testosterone/blood , Adult , Female , Humans , Pregnancy , Pregnancy Trimester, First , Pregnancy Trimester, Second , Pregnancy Trimester, Third , RadioimmunoassaySubject(s)
Adrenal Hyperplasia, Congenital/epidemiology , Female , Humans , Infant, Newborn , Male , Mass Screening , YugoslaviaSubject(s)
Adrenal Cortex Hormones/metabolism , Fluocortolone/therapeutic use , Myasthenia Gravis/drug therapy , Adult , Female , Humans , Hypothalamo-Hypophyseal System/drug effects , Male , Middle Aged , Myasthenia Gravis/metabolism , Myasthenia Gravis/physiopathology , Pituitary-Adrenal System/drug effectsSubject(s)
11-Hydroxycorticosteroids/metabolism , Circadian Rhythm/drug effects , Fluocortolone/administration & dosage , Hydrocortisone/metabolism , Myasthenia Gravis/physiopathology , Pregnadienediols/administration & dosage , 17-Hydroxycorticosteroids/metabolism , Fluocortolone/pharmacology , Humans , Myasthenia Gravis/drug therapyABSTRACT
The effect of alternate-day prednisone regimen (nine-patients) on cortisol secretion rate was compared with daily therapeutic regimen (ten patients) in corticosteroid-dependent asthmatics. Only the alternate-day regimen did not suppress the adrenal function. The therapeutic efficacy of both regimens was equal. The advantages of alternate-day corticosteroid therapy are presented and the optimal way of starting this therapy discussed.
