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1.
Springerplus ; 4: 202, 2015.
Article in English | MEDLINE | ID: mdl-25992309

ABSTRACT

Glycinebetaine (GB) is an important compatible solute for salinity tolerance in many plants. In this study, we analyzed the enzymatic activity and the expression level of betaine aldehyde dehydrogenase (BADH), an important enzyme that catalyzes the last step in the GB synthesis in Leymus chinensis, a GB-hyperaccumulating graminaceous halophyte, and compared with those of barley, a graminaceous glycophyte. We have isolated cDNAs for two BADH genes, LcBADH1 and LcBADH2. LcBADH1 has a putative peroxisomal signal peptide (PTS1) at its C-terminus, while LcBADH2 does not have any typical signal peptide. Using immunofluorescent labeling, we showed that BADH proteins were localized to the cytosol and dot-shaped organelles in the mesophyll and bundle sheath cells of L.chinensis leaves. The affinity of recombinant LcBADH2 for betaine aldehyde was comparable to other plant BADHs, whereas recombinant LcBADH1 showed extremely low affinity for betaine aldehyde, indicating that LcBADH2 plays a major role in GB synthesis in L. chinensis. In addition, the recombinant LcBADH2 protein was tolerant to NaCl whereas LcBADH1 wasn't. The kinetics, subcellular and tissue localization of BADH proteins were comparable between L. chinensis and barley. The activity and expression level of BADH proteins were higher in L. chinensis compared with barley under both normal and salinized conditions, which may be related to the significant difference in the amount of GB accumulation between two plants.

2.
Planta ; 234(6): 1215-26, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21769646

ABSTRACT

Glycine betaine (GB) is a compatible solute accumulated by many plants under various abiotic stresses. GB is synthesized in two steps, choline â†’ betaine aldehyde â†’ GB, where a functional choline-oxidizing enzyme has only been reported in Amaranthaceae (a chloroplastic ferredoxin-dependent choline monooxygenase) thus far. Here, we have cloned a cDNA encoding a choline monooxygenase (CMO) from barley (Hordeum vulgare) plants, HvCMO. In barley plants under non-stress condition, GB had accumulated in all the determined organs (leaves, internodes, awn and floret proper), mostly in the leaves. The expression of HvCMO protein was abundant in the leaves, whereas the expression of betaine aldehyde dehydrogenase (BADH) protein was abundant in the awn, floret proper and the youngest internode than in the leaves. The accumulation of HvCMO mRNA was increased by high osmotic and low-temperature environments. Also, the expression of HvCMO protein was increased by the presence of high NaCl. Immunofluorescent labeling of HvCMO protein and subcellular fractionation analysis showed that HvCMO protein was localized to peroxisomes. [(14)C]choline was oxidized to betaine aldehyde and GB in spinach (Spinacia oleracea) chloroplasts but not in barley, which indicates that the subcellular localization of choline-oxidizing enzyme is different between two plant species. We investigated the choline-oxidizing reaction using recombinant HvCMO protein expressed in yeast (Saccharomyces cerevisiae). The crude extract of HvCMO-expressing yeast coupled with recombinant BBD2 protein converted [(14)C]choline to GB when NADPH was added as a cofactor. These results suggest that choline oxidation in GB synthesis is mediated by a peroxisomal NADPH-dependent choline monooxygenase in barley plants.


Subject(s)
Betaine/metabolism , Gene Expression Regulation, Enzymologic/genetics , Hordeum/enzymology , Oxygenases/metabolism , Peroxisomes/enzymology , Plant Proteins/metabolism , Amino Acid Sequence , Base Sequence , Betaine-Aldehyde Dehydrogenase/genetics , Betaine-Aldehyde Dehydrogenase/metabolism , Choline/metabolism , Cold Temperature , DNA, Complementary/genetics , Gene Expression Regulation, Plant/genetics , Hordeum/genetics , Molecular Sequence Data , Osmotic Pressure , Oxidation-Reduction , Oxygenases/genetics , Plant Leaves/enzymology , Plant Leaves/genetics , Plant Proteins/genetics , Plants, Genetically Modified , RNA, Messenger/genetics , RNA, Plant/genetics , Sequence Alignment , Sequence Analysis, DNA , Spinacia oleracea/genetics , Spinacia oleracea/metabolism
3.
Planta ; 232(1): 133-43, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20376676

ABSTRACT

The accumulation of glycinebetaine (GB) is one of the adaptive strategies to adverse salt stress conditions. Although it has been demonstrated that barley plants accumulate GB in response to salt stress and various studies focused on GB synthesis were performed, its transport mechanism is still unclear. In this study, we identified a novel gene, HvProT2, encoding Hordeum vulgare GB/proline transporter from barley plants. Heterologous expression in yeast (Saccharomyces cerevisiae) mutant demonstrated that the affinity of HvProT2 was highest for GB, intermediate for proline and lowest for gamma-aminobutyric acid. Transient expression of fusions of HvProT2 and green fluorescent protein in onion epidermal cells revealed that HvProT2 is localized at the plasma membrane. Relative quantification of mRNA level of HvProT2 using semi-quantitative reverse transcription-polymerase chain reaction analysis showed that HvProT2 is constitutively expressed in both leaves and roots, and the expression level was higher in old leaves than young leaves and roots. Moreover, we found that HvProT2 was expressed in the mestome sheath and lateral root cap cells. We discussed the possible involvement of HvProT2 for salt stress tolerance.


Subject(s)
Amino Acid Transport Systems, Neutral/genetics , Betaine/metabolism , Glycine/metabolism , Hordeum/genetics , Plant Roots/metabolism , Amino Acid Transport Systems, Neutral/metabolism , Cloning, Molecular , DNA, Complementary , In Situ Hybridization , Kinetics , Reverse Transcriptase Polymerase Chain Reaction , Saccharomyces cerevisiae/genetics , Subcellular Fractions/metabolism
4.
FEBS Lett ; 583(22): 3625-9, 2009 Nov 19.
Article in English | MEDLINE | ID: mdl-19850038

ABSTRACT

Although rice (Oryza sativa L.) produces little glycine betaine (GB), it has two betaine aldehyde dehydrogenase (BADH; EC 1.2.1.8) gene homologs (OsBADH1 and OsBADH2). We found that OsBADH1 catalyzes the oxidation of acetaldehyde efficiently, while the activity of OsBADH2 is extremely low. The accumulation of OsBADH1 mRNA decreases following submergence treatment, but quickly recovers after re-aeration. We confirmed that OsBADH1 localizes in peroxisomes. In this paper, a possible physiological function of OsBADH1 in the oxidation of acetaldehyde produced by catalase in rice plant peroxisomes is discussed.


Subject(s)
Acetaldehyde/metabolism , Betaine-Aldehyde Dehydrogenase/metabolism , Oryza/metabolism , Peroxisomes/metabolism , Plant Proteins/metabolism , Betaine-Aldehyde Dehydrogenase/genetics , Catalysis , Cytosol/metabolism , Gene Expression Profiling , Gene Expression Regulation, Plant , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Isoenzymes/genetics , Isoenzymes/metabolism , Kinetics , Microscopy, Confocal , Oryza/genetics , Oxidation-Reduction , Plant Proteins/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Substrate Specificity
5.
J Plant Physiol ; 166(6): 569-78, 2009 Apr 01.
Article in English | MEDLINE | ID: mdl-18845360

ABSTRACT

Since sweetness is one of the most important qualities of many fruits, and since sugars are translocated from leaves to fruits, the present study investigates photosynthetic activity, activity of sugar metabolizing enzymes, sugar content in leaves and fruits and endogenous levels of hydrogen peroxide in leaves of melon plants treated with various dilutions of hydrogen peroxide, a nonspecific signaling molecule in abiotic stress. For this purpose, 4-month-old melon plants were treated with various concentrations (<50mM) of hydrogen peroxide by applying 300 mL per day to the soil of potted plants. The treatments resulted in increased fructose, glucose, sucrose and starch in the leaves and fruits. The most effective concentration of hydrogen peroxide was 20mM. During the day, soluble sugars in leaves were highest at 12:00 h and starch at 15:00 h. Furthermore, the peroxide treatment increased the photosynthetic activity and the activities of chloroplastic and cytosolic fructose-1,6-bisphosphatase, sucrose phosphate synthase and invertases. Thus, our data show that exogenous hydrogen peroxide, applied to the soil, can increase the soluble sugar content of melon fruits.


Subject(s)
Carbohydrate Metabolism/drug effects , Cucurbitaceae/drug effects , Cucurbitaceae/metabolism , Fruit/drug effects , Fruit/metabolism , Hydrogen Peroxide/pharmacology , Biomass , Carbohydrate Metabolism/radiation effects , Circadian Rhythm/drug effects , Circadian Rhythm/radiation effects , Cucurbitaceae/enzymology , Cucurbitaceae/radiation effects , Fructose-Bisphosphatase/metabolism , Fruit/radiation effects , Glucosyltransferases/metabolism , Hydrogen Peroxide/metabolism , Light , Photosynthesis/drug effects , Photosynthesis/radiation effects , Plant Leaves/drug effects , Plant Leaves/enzymology , Plant Leaves/radiation effects , Plant Stems/drug effects , Plant Stems/metabolism , Plant Stems/radiation effects , Solubility/drug effects , Solubility/radiation effects , Starch/metabolism , beta-Fructofuranosidase/metabolism
6.
Physiol Plant ; 134(1): 22-30, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18429940

ABSTRACT

Betaine aldehyde dehydrogenase (BADH; EC 1.2.1.8) is an important enzyme that catalyzes the last step in the synthesis of glycine betaine, a compatible solute accumulated by many plants under various abiotic stresses. In barley (Hordeum vulgare L.), we reported previously the existence of two BADH genes (BBD1 and BBD2) and their corresponding proteins, peroxisomal BADH (BBD1) and cytosolic BADH (BBD2). To investigate their enzymatic properties, we expressed them in Escherichia coli and purified both proteins. Enzymatic analysis indicated that the affinity of BBD2 for betaine aldehyde was reasonable as other plant BADHs, but BBD1 showed extremely low affinity for betaine aldehyde with apparent K(m) of 18.9 microM and 19.9 mM, respectively. In addition, V(max)/K(m) with betaine aldehyde of BBD2 was about 2000-fold higher than that of BBD1, suggesting that BBD2 plays a main role in glycine betaine synthesis in barley plants. However, BBD1 catalyzed the oxidation of omega-aminoaldehydes such as 4-aminobutyraldehyde and 3-aminopropionaldehyde as efficiently as BBD2. We also found that both BBDs oxidized 4-N-trimethylaminobutyraldehyde and 3-N-trimethylaminopropionaldehyde.


Subject(s)
Betaine-Aldehyde Dehydrogenase/metabolism , Hordeum/enzymology , Peroxisomes/metabolism , Plant Proteins/metabolism , Cytosol/enzymology , Substrate Specificity
7.
Planta ; 227(2): 277-86, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17828417

ABSTRACT

A compatible solute, proline is accumulated in various kinds of plants and microorganisms under environmental stresses. The function of proline is thought to be an osmotic regulator under water stress, and its transport into cells is mediated by a proline transporter. Here, we report the effects of expressing the barley proline transporter (HvProT) under the control of either the CaMV35S promoter (35Sp) or a root cap promoter (RCp), on Arabidopsis growth. In Arabidopsis, transformed HvProT functions in the plasma membrane, like other amino acid transporters. Reduction in biomass production was observed in aerial parts of 35Sp-HvProT plants, and it was accompanied with decreased proline accumulation in leaves. Impaired growth of 35Sp-HvProT plants was restored by exogenously adding L: -proline. These results suggested that growth reduction was caused by a deficiency of endogenous proline. In 35Sp-HvProT plants, the amount of proline dehydrogenase (PDH) transcript was increased compared to wild type (WT) plants, with a consequent enhancement of the activity of PDH. On the other hand, the transgenic RCp-HvProT plants accumulated 2- to 3-fold more proline in the root tip region compared to WT, and root elongation was enhanced at the same time. Thus, different physiological responses were caused by the altered location in accumulation of proline using two different promoters for heterologous expression of HvProT. These results indicate the importance of proline distribution at the tissue level during vegetative development.


Subject(s)
Amino Acid Transport Systems, Neutral/genetics , Amino Acid Transport Systems, Neutral/metabolism , Arabidopsis/growth & development , Arabidopsis/metabolism , Hordeum/genetics , Arabidopsis/genetics , Gene Expression Regulation, Plant , Molecular Sequence Data , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Roots/growth & development , Plant Roots/metabolism , Plants, Genetically Modified
9.
Biochem Biophys Res Commun ; 355(1): 61-6, 2007 Mar 30.
Article in English | MEDLINE | ID: mdl-17286958

ABSTRACT

Accumulation of an osmoprotectant, proline, is enhanced in response to salinity in plants. Here, by immunohistochemical analysis, we demonstrated that proline transporter (HvProT) was highly expressed in the apical region of barley roots under salt stress. Free proline was accumulated more in the basal region than in the apical region of barley roots under salt stress, although expression level of HvProT was higher in the apical region. On the other hand, salt stress increased proline and hydroxyproline contents in the cell wall fraction of the root apical region, suggesting increment of proline utilization. Expression of the genes encoding cell wall proteins (proline rich protein and extensin) and cellulose synthase was induced in barley roots by salt stress. These findings indicated that free proline transported by HvProT presumably behaved as a component of cell wall synthesis in the apical region of barley roots under salt stress.


Subject(s)
Hordeum/metabolism , Hydroxyproline/metabolism , Plant Roots/metabolism , Proline/metabolism , Sodium Chloride/pharmacology , Blotting, Northern , Cell Wall/drug effects , Cell Wall/metabolism , Hordeum/drug effects , Hordeum/growth & development , Immunohistochemistry , Plant Roots/drug effects , RNA, Plant/genetics , RNA, Plant/isolation & purification
10.
Ann Bot ; 98(3): 565-71, 2006 Sep.
Article in English | MEDLINE | ID: mdl-16790464

ABSTRACT

BACKGROUND AND AIMS: Glycinebetaine (GB), a quaternary ammonium compound, is a very effective compatible solute. In higher plants, GB is synthesized from choline (Cho) via betaine aldehyde (BA). The first and second steps in the biosynthesis of GB are catalysed by choline monooxygenase (CMO) and by betaine aldehyde dehydrogenase (BADH), respectively. Rice (Oryza sativa), which has two genes for BADH, does not accumulate GB because it lacks a functional gene for CMO. Rice plants accumulate GB in the presence of exogenously applied BA, which leads to the development of a significant tolerance to salt, cold and heat stress. The goal in this study was to evaluate and to discuss the effects of endogenously accumulated GB in rice. METHODS: Transgenic rice plants that overexpressed a gene for CMO from spinach (Spinacia oleracea) were produced by Agrobacterium-mediated transformation. After Southern and western blotting analysis, GB in rice leaves was quantified by (1)H-NMR spectroscopy and the tolerance of GB-accumulating plants to abiotic stress was investigated. KEY RESULTS: Transgenic plants that had a single copy of the transgene and expressed spinach CMO accumulated GB at the level of 0.29-0.43 micromol g(-1) d. wt and had enhanced tolerance to salt stress and temperature stress in the seedling stage. CONCLUSIONS: In the CMO-expressing rice plants, the localization of spinach CMO and of endogenous BADHs might be different and/or the catalytic activity of spinach CMO in rice plants might be lower than it is in spinach. These possibilities might explain the low levels of GB in the transgenic rice plants. It was concluded that CMO-expressing rice plants were not effective for accumulation of GB and improvement of productivity.


Subject(s)
Betaine/metabolism , Oryza/metabolism , Oxygenases/metabolism , Sodium Chloride/metabolism , Betaine/chemistry , Blotting, Southern , Blotting, Western , Genes, Plant , Magnetic Resonance Spectroscopy , Oryza/genetics , Oxygenases/deficiency , Oxygenases/genetics , Plant Leaves/genetics , Plants, Genetically Modified , Rhizobium/genetics , Spinacia oleracea/enzymology , Spinacia oleracea/genetics , Temperature , Transformation, Genetic
11.
Theor Appl Genet ; 112(7): 1286-94, 2006 May.
Article in English | MEDLINE | ID: mdl-16496119

ABSTRACT

Although barley and rice belong to the same family Poaceae, they differ in their ability to tolerate salt stress. In an attempt to understand the molecular bases of such differences, we compared changes in transcriptome between barley and rice in response to salt stress using barley cDNA microarrays. At 1 and 24 h after salt stress, many genes were up-regulated in barley, but not in rice. Leaf water potential declined in the first 10 h of stress in both species, but recovered in the period 24-48 h only in barley. In addition, we found that barley partitioned Na+ to the roots and away from the shoots more efficiently than rice. These differences in physiological responses were correlated with the differences in the steady-state abundance of transcripts for the genes related to adaptive functions. Transcripts for plasma membrane protein 3 and inorganic pyrophosphatase were up-regulated in both species, but only transiently in rice. This indicates that adaptive mechanisms for regulating ion homeostasis are partly conserved in the two species, but it seems that rice cannot sustain cellular ion homeostasis for a long time like barley. These results imply that genetic modification of regulatory controls of early salt-responsive genes might lead to development of the salt tolerance trait in rice.


Subject(s)
Gene Expression Regulation, Plant/drug effects , Genes, Plant , Hordeum/genetics , Oryza/genetics , Sodium Chloride/pharmacology , Hordeum/drug effects , Hordeum/metabolism , Oligonucleotide Array Sequence Analysis , Oryza/drug effects , Oryza/metabolism , Plant Leaves/drug effects , Plant Roots/drug effects , Time Factors , Transcription, Genetic
12.
Appl Environ Microbiol ; 71(8): 4176-84, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16085800

ABSTRACT

Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium which can grow at NaCl concentrations up to 3.0 M and at pH values up to 11. The genome sequence revealed that the cyanobacterium Synechocystis sp. strain PCC 6803 contains five putative Na+/H+ antiporters, two of which are homologous to NhaP of Pseudomonas aeruginosa and three of which are homologous to NapA of Enterococcus hirae. The physiological and functional properties of NapA-type antiporters are largely unknown. One of NapA-type antiporters in Synechocystis sp. strain PCC 6803 has been proposed to be essential for the survival of this organism. In this study, we examined the isolation and characterization of the homologous gene in Aphanothece halophytica. Two genes encoding polypeptides of the same size, designated Ap-napA1-1 and Ap-napA1-2, were isolated. Ap-NapA1-1 exhibited a higher level of homology to the Synechocystis ortholog (Syn-NapA1) than Ap-NapA1-2 exhibited. Ap-NapA1-1, Ap-NapA1-2, and Syn-NapA1 complemented the salt-sensitive phenotypes of an Escherichia coli mutant and exhibited strongly pH-dependent Na+/H+ and Li+/H+ exchange activities (the highest activities were at alkaline pH), although the activities of Ap-NapA1-2 were significantly lower than the activities of the other polypeptides. Only one these polypeptides, Ap-NapA1-2, complemented a K+ uptake-deficient E. coli mutant and exhibited K+ uptake activity. Mutagenesis experiments suggested the importance of Glu129, Asp225, and Asp226 in the putative transmembrane segment and Glu142 in the loop region for the activity. Overexpression of Ap-NapA1-1 in the freshwater cyanobacterium Synechococcus sp. strain PCC 7942 enhanced the salt tolerance of cells, especially at alkaline pH. These findings indicate that A. halophytica has two NapA1-type antiporters which exhibit different ion specificities and play an important role in salt tolerance at alkaline pH.


Subject(s)
Cyanobacteria/metabolism , Sodium Chloride/pharmacology , Sodium-Hydrogen Exchangers/metabolism , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Culture Media , Cyanobacteria/drug effects , Cyanobacteria/physiology , Escherichia coli/genetics , Escherichia coli/metabolism , Gene Expression Regulation, Bacterial , Hydrogen-Ion Concentration , Lithium Chloride/pharmacology , Molecular Sequence Data , Mutagenesis, Site-Directed , Potassium/metabolism , Sodium-Hydrogen Exchangers/chemistry , Sodium-Hydrogen Exchangers/genetics
13.
Planta ; 222(6): 1001-9, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16034593

ABSTRACT

For plant salt tolerance, it is important to regulate the uptake and accumulation of Na+ ions. The yeast pmp3 mutant which lacks PMP3 gene accumulates excess Na+ ions in the cell and shows increased Na+ sensitivity. Although the function of PMP3 is not fully understood, it is proposed that PMP3 contributes to the restriction of Na+ uptake and consequently salt tolerance in yeasts. In this paper, we have investigated whether the lack of RCI2A gene, homologous to PMP3 gene, causes a salt sensitive phenotype in Arabidopsis (Arabidopsis thaliana (L.) Heynh.) plants; and to thereby indicate the physiological role of RCI2A in higher plants. Two T-DNA insertional mutants of RCI2A were identified. Although the growth of rci2a mutants was comparable with that of wild type under normal conditions, high NaCl treatment caused increased accumulation of Na+ and more reduction of the growth of roots and shoots of rci2a mutants than that of wild type. Undifferentiated callus cultures regenerated from rci2a mutants also accumulated more Na+ than that from wild type under high NaCl treatment. Furthermore, when wild-type and rci2a plants were treated with NaCl, NaNO3, Na2SO4, KCl, KNO3, K2SO4 or LiCl, the rci2a mutants showed more reduction of shoot growth than wild type. Under treatments of tetramethylammonium chloride, CaCl2, MgCl2, mannitol or sorbitol, the growth reduction was comparable between wild-type and rci2a plants. These results suggested that RCI2A plays a role directly or indirectly for avoiding over-accumulation of excess Na+ and K+ ions in plants, and contributes to salt tolerance.


Subject(s)
Arabidopsis Proteins/physiology , Arabidopsis/physiology , Heat-Shock Proteins/physiology , Membrane Proteins/physiology , Sodium/metabolism , Arabidopsis/drug effects , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , DNA, Bacterial , Gene Expression/drug effects , Heat-Shock Proteins/genetics , Membrane Proteins/genetics , Mutagenesis, Insertional , Sodium Chloride/pharmacology
14.
Proc Natl Acad Sci U S A ; 102(5): 1318-23, 2005 Feb 01.
Article in English | MEDLINE | ID: mdl-15665084

ABSTRACT

Betaine is an important osmoprotectant, synthesized by many plants in response to abiotic stresses. Almost all known biosynthetic pathways of betaine are two-step oxidations of choline. Recently, a biosynthetic pathway of betaine from glycine, catalyzed by two N-methyltransferase enzymes, was found. Here, the potential role of N-methyltransferase genes for betaine synthesis was examined in a freshwater cyanobacterium, Synechococcus sp. PCC 7942, and in Arabidopsis plants. It was found that the coexpression of N-methyltransferase genes in Synechococcus caused accumulation of a significant amount of betaine and conferred salt tolerance to a freshwater cyanobacterium sufficient for it to become capable of growth in seawater. Arabidopsis plants expressing N-methyltransferase genes also accumulated betaine to a high level in roots, stems, leaves, and flowers and improved seed yield under stress conditions. Betaine levels were higher than those produced by choline-oxidizing enzymes. These results demonstrate the usefulness of glycine N-methyltransferase genes for the improvement of abiotic stress tolerance in crop plants.


Subject(s)
Arabidopsis/metabolism , Betaine/metabolism , Glycine/metabolism , Synechococcus/metabolism , Arabidopsis/genetics , Methylation , Methyltransferases/genetics , Osmolar Concentration , Plants, Genetically Modified/metabolism , Synechococcus/genetics
15.
Planta ; 220(3): 395-402, 2005 Jan.
Article in English | MEDLINE | ID: mdl-15580528

ABSTRACT

Regulation of ion homeostasis is fundamental to physiological activities in plants. Here, we report on the functional characterization of AcPMP3 [Aneurolepidium chinense (a monocotyledonous halophyte) plasma membrane protein 3] under salt stress. Expression of AcPMP3-1 and AcPMP3-2 genes was highly induced by various abiotic stresses, such as salt, cold and drought. Furthermore, abscisic acid, H(2)O(2) and salicylic acid also triggered expression of AcPMP3 genes. In the Deltanha1 Deltapmr2 Deltapmp3 yeast mutant, which lacks the major Na(+) efflux systems (Na(+)/H(+) antiporter and Na(+)-ATPase), its salt-sensitive phenotype was restored by expressing the AcPMP3-1 gene, and the transformants accumulated lesser amounts of Na(+) and K(+) than mutant cells under 50 mM NaCl and 500 mM KCl conditions, respectively. These results suggested that AcPMP3-1 plays a role as a regulator of both Na(+) and K(+) accumulation in the cells. In situ hybridization showed that the AcPMP3-1 transcript was localized in cells of the root cap and root epidermis, which strongly suggested that AcPMP3-1 is essential for regulating Na(+)/K(+) transportation between plant roots and the outer environment under salt stress.


Subject(s)
Membrane Proteins/metabolism , Plant Proteins/metabolism , Poaceae/chemistry , Sodium Chloride/metabolism , Amino Acid Sequence , Circadian Rhythm , Gene Expression Profiling , Gene Expression Regulation, Plant/physiology , Membrane Proteins/genetics , Molecular Sequence Data , Osmotic Pressure , Poaceae/genetics , Potassium Chloride/metabolism , Recombinant Fusion Proteins/metabolism , Saccharomyces cerevisiae , Sequence Homology, Amino Acid
16.
J Exp Bot ; 55(406): 2213-8, 2004 Oct.
Article in English | MEDLINE | ID: mdl-15361537

ABSTRACT

Under high salt conditions, plant growth is severely inhibited due to both osmotic and ionic stresses. In an effort to dissect genes and pathways that respond to changes in osmotic potential under salt stress, the expression patterns were compared of 460 non-redundant salt-responsive genes in barley during the initial phase under osmotic versus salt stress using cDNA microarrays with northern blot and real-time RT-PCR analyses. Out of 52 genes that were differentially expressed under osmotic stress, 11, such as the up-regulated genes for pyrroline-5-carboxylate synthetase, betaine aldehyde dehydrogenase 2, plasma membrane protein 3, and the down-regulated genes for water channel 2, heat shock protein 70, and phospholipase C, were regulated in a virtually identical manner under salt stress. These genes were involved in a wide range of metabolic and signalling pathways suggesting that, during the initial phase under salt stress, several of the cellular responses are mediated by changes in osmotic potential.


Subject(s)
Gene Expression Regulation, Plant/physiology , Hordeum/genetics , Sodium Chloride/pharmacology , Enzymes/genetics , Gene Expression Regulation, Plant/drug effects , Hordeum/drug effects , Hordeum/enzymology , Oligonucleotide Array Sequence Analysis , Osmolar Concentration , Reproducibility of Results
17.
J Biol Chem ; 279(6): 4330-8, 2004 Feb 06.
Article in English | MEDLINE | ID: mdl-14559898

ABSTRACT

Genome sequences of cyanobacteria, Synechocystis sp. PCC 6803, Anabaena sp. PCC 7120, and Thermosynechococcus elongatus BP-1 revealed the presence of a single Ca2+/H+ antiporter in these organisms. Here, we isolated the putative Ca2+/H+ antiporter gene from Synechocystis sp. PCC 6803 (synCAX) as well as a homologous gene from a halotolerant cyanobacterium Aphanothece halophytica (apCAX). In contrast to plant vacuolar CAXs, the full-length apCAX and synCAX genes complemented the Ca2+-sensitive phenotype of an Escherichia coli mutant. ApCAX and SynCAX proteins catalyzed specifically the Ca2+/H+ exchange reaction at alkaline pH. Immunological analysis suggested their localization in plasma membranes. The Synechocystis sp. PCC 6803 cells disrupted of synCAX exhibited lower Ca2+ efflux activity and a salt-sensitive phenotype. Overexpression of ApCAX and SynCAX enhanced the salt tolerance of Synechococcus sp. PCC 7942 cells. Mutagenesis analyses indicate the importance of two conserved acidic amino acid residues, Glu-74 and Glu-324, in the transmembrane segments for the exchange activity. These results clearly indicate that cyanobacteria contain a Ca2+/H+ antiporter in their plasma membranes, which plays an important role for salt tolerance.


Subject(s)
Antiporters/isolation & purification , Antiporters/metabolism , Bacterial Proteins/isolation & purification , Bacterial Proteins/metabolism , Calcium-Binding Proteins/isolation & purification , Calcium-Binding Proteins/metabolism , Cation Transport Proteins/isolation & purification , Cation Transport Proteins/metabolism , Cyanobacteria/metabolism , Amino Acid Sequence , Anabaena/genetics , Anabaena/metabolism , Antiporters/genetics , Bacterial Proteins/genetics , Base Sequence , Calcium-Binding Proteins/genetics , Cation Transport Proteins/genetics , Cell Membrane/metabolism , Cloning, Molecular , Cyanobacteria/drug effects , Cyanobacteria/genetics , DNA, Bacterial/genetics , Escherichia coli/genetics , Genes, Bacterial , Genetic Complementation Test , Hydrogen-Ion Concentration , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Phenotype , Phylogeny , Protein Structure, Secondary , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Sequence Homology, Amino Acid , Sodium Chloride/pharmacology , Species Specificity
18.
J Biol Chem ; 278(7): 4932-42, 2003 Feb 14.
Article in English | MEDLINE | ID: mdl-12466265

ABSTRACT

Glycine betaine (N,N,N-trimethylglycine) is an important osmoprotectant and is synthesized in response to abiotic stresses. Although almost all known biosynthetic pathways of betaine are two-step oxidation of choline, here we isolated two N-methyltransferase genes from a halotolerant cyanobacterium Aphanothece halophytica. One of gene products (ORF1) catalyzed the methylation reactions of glycine and sarcosine with S-adenosylmethionine acting as the methyl donor. The other one (ORF2) specifically catalyzed the methylation of dimethylglycine to betaine. Both enzymes are active as monomers. Betaine, a final product, did not show the feed back inhibition for the methyltransferases even in the presence of 2 m. A reaction product, S-adenosyl homocysteine, inhibited the methylation reactions with relatively low affinities. The co-expressing of two enzymes in Escherichia coli increased the betaine level and enhanced the growth rates. Immunoblot analysis revealed that the accumulation levels of both enzymes in A. halophytica cells increased with increasing the salinity. These results indicate that A. halophytica cells synthesize betaine from glycine by a three-step methylation. The changes of amino acids Arg-169 to Lys or Glu in ORF1 and Pro-171 to Gln and/or Met-172 to Arg in ORF2 significantly decreased V(max) and increased K(m) for methyl acceptors (glycine, sarcosine, and dimethylglycine) but modestly affected K(m) for S-adenosylmethionine, indicating the importance of these amino acids for the binding of methyl acceptors. Physiological and functional properties of methyltransferases were discussed.


Subject(s)
Betaine/metabolism , Cyanobacteria/enzymology , Glycine/metabolism , Methyltransferases , Amino Acid Sequence , Bacterial Proteins/analysis , Bacterial Proteins/isolation & purification , Bacterial Proteins/metabolism , Catalytic Domain , Methyltransferases/analysis , Methyltransferases/isolation & purification , Methyltransferases/metabolism , Molecular Sequence Data , Sequence Alignment , Substrate Specificity
19.
J Biol Chem ; 277(21): 18373-82, 2002 May 24.
Article in English | MEDLINE | ID: mdl-11907031

ABSTRACT

Betaine is an important osmoprotectant in many plants, but its transport activity has only been demonstrated using a proline transporter from tomato, a betaine-nonaccumulating plant. In this study, two full-length and one partial transporter genes were isolated from betaine-accumulating mangrove Avicennia marina. Their homologies to betaine transporters from bacteria and betaine/4-aminobutyrate transporters from mammalian cells were low but were high to proline transporters from Arabidopsis and tomato. Two full-length transporters could complement the Na(+)-sensitive phenotype of the Escherichia coli mutant deficient in betT, putPA, proP, and proU. Both transporters could efficiently take up betaine and proline with similar affinities (K(m), 0.32-0.43 mm) and maximum velocities (1.9-3.6 nmol/min/mg of protein). The uptakes of betaine and proline were significantly inhibited by mono- and dimethylglycine but only partially inhibited by betaine aldehyde, choline, and 4-aminobutyrate. Sodium and potassium chloride markedly enhanced betaine uptake rates with optimum concentrations at 0.5 m, whereas sucrose showed only modest activation. The change of amino acids Thr(290)-Thr-Ser(292) in a putative periplasmic loop to Arg(290)-Gly-Arg(292) yielded the active transporter independent of salts, suggesting the positive charge induced a conformational change to the active form. These data clearly indicate that the betaine-accumulating mangrove contains betaine/proline transporters whose properties are distinct from betaine transporters of bacteria and mammalian cells.


Subject(s)
Amino Acid Transport Systems, Neutral/physiology , Betaine/metabolism , Carrier Proteins/physiology , Trees/metabolism , Amino Acid Sequence , Amino Acid Transport Systems, Neutral/chemistry , Amino Acid Transport Systems, Neutral/genetics , Carrier Proteins/chemistry , Carrier Proteins/genetics , Cloning, Molecular , GABA Plasma Membrane Transport Proteins , Kinetics , Molecular Sequence Data , Proline/metabolism , Sequence Homology, Amino Acid
20.
J Plant Res ; 115(1118): 119-30, 2002 Apr.
Article in English | MEDLINE | ID: mdl-12884135

ABSTRACT

To obtain insight into the comprehensive molecular characteristics related to the mechanisms of salt tolerance, we performed a large-scale screening of salt-inducible genes in barley roots by differential display. A comparative analysis of gene expression between control and salt-stressed conditions led to the detection of 218 cDNA clones induced by salt. Sequence analysis and database searching revealed that 133 cDNA clones have homology to known proteins. Twenty-four salt-inducible clones were identified as genes for signal transduction (e.g., phosphatidylinositol-4-phosphate-5-kinase, mitogen-activated protein kinase, transcription factor, receptor protein kinase, and protein phosphatase 2A). We also detected clones encoding glutathione reductase, thioredoxin-like protein, trehalose-6-phosphate synthetase, and heat shock proteins in the category of typical stress tolerance. Furthermore, we have obtained genes encoding membrane transporters, members of the P450 family, enzymes involved in RNA metabolism or function, and enzymes of sugar or amino acid metabolism. It must be noted that most genes were expressed strongly in roots, but only rarely or weakly in leaves. In addition, some clones were newly found as salt-inducible genes encoding SCARECROW, splicing factor and apoptosis protein. In this research, it was shown that differential display is a powerful tool for a large-scale cloning of cDNAs induced by salt and these results are very useful for understanding the mechanisms of plant salt tolerance.

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