ABSTRACT
Effects of α-ketol linolenic acid (KODA) application on endogenous abscisic acid (ABA), jasmonic acid (JA), and aromatic volatiles were investigated in 'Kyoho' grapes (Vitis labrusca×Vitis vinifera) infected by a pathogen (Glomerella cingulata). The expressions of 9-cis-epoxycarotenoid dioxygenase (VvNCED1), ABA 8'-hydroxylase (VvCYP707A1), lipoxygenase (VvLOX), and allene oxide synthase (VvAOS) were also examined. The grape berries were dipped in 0.1mM KODA solution before inoculation with the pathogen and stored at 25°C for 12 days. The development of infection was significantly suppressed upon KODA treatment. Endogenous ABA, JA and phaseic acid (PA) were induced in inoculated berries. KODA application before inoculation increased endogenous ABA, PA and JA through the activation of VvNCED1, VvCYP707A1 and VvAOS genes, respectively. In addition, terpenes, methyl salicylate (Me-SA) and C6-aldehydes such as (E)-2-hexenal and cis-3-hexenal associated with fungal resistance also increased in KODA-treated berries during storage. These results suggest that the synergistic effect of JA, ABA, and some aromatic volatiles induced by KODA application may provide resistance to pathogen infection in grape berries.
Subject(s)
Arabidopsis Proteins/metabolism , Phyllachorales/physiology , Plant Diseases/microbiology , Plant Growth Regulators/metabolism , Signal Transduction , Vitis/genetics , Abscisic Acid/metabolism , Aldehydes/metabolism , Antioxidants/metabolism , Arabidopsis Proteins/genetics , Cyclopentanes/metabolism , Cytochrome P-450 Enzyme System/genetics , Cytochrome P-450 Enzyme System/metabolism , Dioxygenases/genetics , Dioxygenases/metabolism , Fruit/genetics , Fruit/immunology , Fruit/microbiology , Fruit/physiology , Intramolecular Oxidoreductases/genetics , Intramolecular Oxidoreductases/metabolism , Lipoxygenase/genetics , Lipoxygenase/metabolism , Oxylipins/metabolism , Plant Diseases/immunology , Plant Proteins/genetics , Plant Proteins/metabolism , Vitis/immunology , Vitis/microbiology , Vitis/physiology , alpha-Linolenic Acid/metabolismABSTRACT
Holoparasitic plants such as Orobanche spp. have lost their photosynthetic ability, so photoresponses to optimize photosynthesis are not necessary in these plants. Photoresponses are also involved in the regulation of plant development but the photoresponses of holoparasites have not been characterized in detail. In this study, the phytochrome (phy)-related photoresponse of Orobanche minor was investigated. Its photoreceptor, phytochrome A (OmphyA), was also characterized. Light effects on germination, shoot elongation, anthocyanin biosynthesis, and OmphyA expression and subcellular localization were analyzed. Red light (R):far-red light (FR) reversible inhibition of O. minor seed germination demonstrated that phy-mediated responses are retained in this holoparasite. Shoot elongation was inhibited by FR but not by R. This pattern is unique among known patterns of plant photoresponses. Additionally, molecular analysis showed that OmphyA is able to respond to the light signals. Interestingly, the unique pattern of photoresponses in O. minor seems to have been modified for adaptation to its parasitic life cycle. We hypothesize that this alteration has resulted from the loss or alteration of some phy-signaling components. Elucidation of altered components in phy signaling in this parasite will provide useful information not only about its physiological characteristics but also about general plant photoreception systems.
Subject(s)
Orobanche/metabolism , Parasites/metabolism , Phytochrome A/metabolism , Animals , Anthocyanins/biosynthesis , Blotting, Western , Gene Expression Regulation, Plant , Germination/radiation effects , Green Fluorescent Proteins/metabolism , Light , Orobanche/genetics , Orobanche/radiation effects , Parasites/genetics , Parasites/radiation effects , Photoperiod , Phytochrome A/genetics , Plant Shoots/genetics , Plant Shoots/growth & development , Plant Shoots/radiation effects , Protein Transport/radiation effects , Protoplasts/metabolism , Protoplasts/radiation effects , Seeds/growth & development , Seeds/radiation effects , Subcellular Fractions/metabolism , Subcellular Fractions/radiation effects , Time FactorsABSTRACT
The progenitors of damascenone (1), the most intensive C13-norisoprenoid volatile aroma constituent of rose essential oil, were surveyed in the flowers of Rosa damascena Mill. Besides 9-O-beta-D-glucopyranosyl-3-hydroxy-7,8-didehydro-beta-ionol (4b), a stable progenitor already isolated from the residual water after steam distillation of flowers of R. damascena Mill., two labile progenitors were identified to be (3S, 9R)- and (3S, 9S)-megastigma-6,7-dien-3,5,9-triol 9-O-beta-D-glucopyranosides (2b) based on their synthesis and HPLC-MS analytical data. Compound 2b gave damascenone (1), 3-hydroxy-beta-damascone (3) and 4b upon heating under acidic conditions.
