Simplified insecticide toxicity determination method for Nesidiocoris tenuis using contaminated diet.

Nesidiocoris tenuis is a zoophytophagous mirid bug widely studied for its role in preying on major greenhouse pests. Since N. tenuis has now been known for its vigorous predation potential on pests such as Bemisia tabaci, many applied entomologists are now recommending that N. tenuis be merged into the Integrated Pest management (IPM) systems. However, successful integration of N. tenuis into any IPM system depends on thorough evaluation with compatible pesticides, as incompatible pesticides can offset the whole idea of IPM. Here, we simulate the field situation where N. tenuis feeds directly on a contaminated B. tabaci nymph or leaves. However, instead of using live B. tabaci nymphs, we used brine shrimp eggs, Artemia salina (Linnaeus, 1758). Brine shrimp eggs have been reported to be an excellent factitious supplementary diet in augmenting N. tenuis populations. Thus, we use brine shrimp eggs to determine the toxicity of pesticides, to which the calculated mortality rates can be used to determine which pesticides can be used together with N. tenuis in an IPM system against any related pest.•We developed a customized containment system that promotes aeration and minimize contamination.•Pesticide contaminated hatched brine shrimp eggs is delivered to N. tenuis in the aerated containment system.•In addition to established methods such as leaf dipping or insect dipping, this method shows to mimic N. tenuis feeding on contaminated B. tabaci nymphs in field conditions thus, predicts how a pesticide may be of toxic or compatible with N. tenuis when both are integrated together.

Augmenting Nesidiocoris tenuis (Nesidiocoris) with a Factitious Diet of Artemia Cysts to Control Bemisia tabaci (Gennadius) on Tomato Plants under Greenhouse Conditions.

Natural predators such as Nesidiocoris tenuis are known for their role in managing greenhouse pests. However, techniques in maximizing the biological control potential of N. tenuis under field conditions are still lacking. We evaluated under greenhouse conditions the prospects of Artemia cysts enhanced with high fructose corn syrup and honey, and delivered using hemp strings (hemp rope) as supplementary factitious dietary in augmenting the proliferation and spread of N. tenuis on tomato plants. Results showed that N. tenuis supplemented with hemp rope could establish, proliferate and disperse among tomato plants compared to the N. tenuis supplemented with banker plants. Even though N. tenuis proliferated exponentially on banker plants, their movement and relocation to tomato plants, as expected, were only congested on tomato plants near the banker plants. However, as the survey continued, they relocated to the rest of the tomato plants. Furthermore, the number of Bemisia tabaci eggs and nymphs, a serious greenhouse pest of tomato, was observed to be significantly reduced in hemp rope greenhouse compared to banker plants and the negative control (no pest control system) greenhouses. This study, therefore, establishes foundational data on the usage of Artemia cysts enhanced with isomerized sugar (high fructose corn syrup) and honey under greenhouse conditions as factitious supplementary dietary in supporting N. tenuis establishment and spread, traits that are essential towards development of whitefly Integrated Pest Management (IPM) system. enhanced with isomerized sugar (high fructose corn syrup) and honey.

Augmentation and compatibility of Beauveria bassiana with pesticides against different growth stages of Bemisia tabaci (Gennadius); an in vitro and field approach.

BACKGROUND: Bemisia tabaci is a notorious agricultural pest that causes serious damage to many crops via herbivory and spread of viral diseases. Effective control measures are, therefore, required. Integrating entomopathogenic fungi into the chemical control system offers promise for B. tabaci management. RESULTS: In-vitro assays on the augmentative effect of Beauveria bassiana GHA strain with insecticides, and its compatibility with fungicides were tested. Varying egg mortality was observed for most insecticides except for milbemectin. Pyrifluquinazon was less effective against the nymphs. Flonicamid showed no insecticidal effect on either nymphs or adults. However, increased mortality in nymphs and adults was observed when flonicamid was mixed with Beauveria bassiana GHA strain. Furthermore, no significant synergistic effect of mixing pesticides with B. bassiana GHA strain was observed. Most insecticides (when not mixed with B. bassiana) showed high (>80%) mortality against B. tabaci nymphs and adults. Most fungicides tested showed no inhibitory effects on B. bassiana GHA strain against B. tabaci nymphs and adults. Fungistatic effect was observed in the mycelial and spore germination inhibition studies. Weekly rotation of some pesticides with B. bassiana GHA strain in greenhouse conditions yielded significant reduction in different growth stages of B. tabaci. However, no significant difference in viral incidence was observed. CONCLUSION: Laboratory and field tests showed positive effects of augmenting pesticides and B. bassiana GHA strain against B. tabaci nymphs and adults. Therefore, augmentation of pesticides and B. bassiana GHA strain is one prospect towards developing an effective B. tabaci IPM system. © 2020 Society of Chemical Industry.

In situ ruminal degradation and in vitro fermentation characteristics, and antioxidative activities of the lotus rhizome.

We evaluated the lotus rhizome as a potential ruminant feed by investigating its compositional properties, in situ degradation profile and in vitro fermentation characteristics with ruminal microbes, in comparison with cereal grains (corn, barley and wheat). The antioxidative activities in the lotus rhizome were also estimated. The soluble fraction of dry matter in lotus tuber was >70%, which was higher than those in the grains. The insoluble fraction in lotus tuber was not degraded by ruminal microbes in accord with a first-order reaction. In an in vitro experiment, lotus tuber showed lower fermentation at 8 hr compared to the grains, but exhibited higher productions of gas and VFA at 48 hr along with a lower lactate and higher pH. The lower value of final lactate production in lotus tuber, indicating the metabolic capacity for lactate utilization retained, suggests a lower risk of ruminal acidosis compared to grains. Lotus rhizome had high antioxidant activities, with the foliar bud showing the strongest ferric reducing antioxidant power, followed in order by the apical bud, node, residual tuber, edible tuber, and nodal root. For ruminants, the lotus rhizome could thus be not only an energy feed but also the source of natural antioxidants.

T929I and K1774N mutation pair and M918L single mutation identified in the voltage-gated sodium channel gene of pyrethroid-resistant Thrips tabaci (Thysanoptera: Thripidae) in Japan.

Pyrethroid-resistance in onion thrips, Thrips tabaci, has been reported in many countries including Japan. Identifying factors of the resistance is important to correctly monitoring the resistance in field populations. To identify pyrethroid-resistance related genes in T. tabaci in Japan, we performed RNA-Seq analysis of seven T. tabaci strains including two pyrethroid-resistant and five pyrethroid-susceptible strains. We identified a pair of single point mutations, T929I and K1774N, introducing two amino acid mutations, in the voltage-gated sodium channel gene, a pyrethroid target gene, in the two resistant strains. The K1774N is a newly identified mutation located in the fourth repeat domain of the sodium channel. Genotyping analysis of field-collected populations showed that most of the T. tabaci individuals in resistant populations carried the mutation pair, indicating that the mutation pair is closely associated with pyrethroid-resistance in Japan. Another resistance-related mutation, M918L, was also identified in part of the resistant populations. Most of the individuals with the mutation pair were arrhenotokous while all individuals with the M918L single mutation were thelytokous. The result of differentially expressed gene analysis revealed a small number of up-regulated detoxification genes in each resistant strain which might be involved in resistance to pyrethroid. However, no up-regulated detoxification genes common to the two resistant strains were detected. Our results indicate that the mutation pair in the sodium channel gene is the most important target for monitoring pyrethroid-resistance in T. tabaci, and that pyrethroid-resistant arrhenotokous individuals with the mutation pair are likely to be widely distributed in Japan.