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1.
Nucleic Acids Res ; 36(18): 5812-21, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18782830

ABSTRACT

Short interfering RNAs (siRNAs) are valuable reagents for sequence-specific inhibition of gene expression via the RNA interference (RNAi) pathway. Although it has been proposed that the relative thermodynamic stability at the 5'-ends of siRNAs plays a crucial role in siRNA strand selection, we demonstrate here that a character of the 2-nt 3'-overhang of siRNAs is the predominant determinant of which strand participates in the RNAi pathway. We show that siRNAs with a unilateral 2-nt 3'-overhang on the antisense strand are more effective than siRNAs with 3'-overhangs at both ends, due to preferential loading of the antisense strand into the RNA-induced silencing complex (RISC). Regardless of the relative thermodynamic stabilities at the ends of siRNAs, overhang-containing strands are predominantly selected as the guide strand; whereas, relative stability markedly influences opposite strand selection. Moreover, we show that sense strand modifications, such as deletions or DNA substitutions, of siRNAs with unilateral overhang on the antisense strand have no negative effect on the antisense strand selection, but may improve RNAi potency. Our findings provide useful guidelines for the design of potent siRNAs and contribute to understanding the crucial factors in determining strand selection in mammalian cells.


Subject(s)
RNA Interference , RNA, Small Interfering/chemistry , DNA/chemistry , HeLa Cells , Humans , RNA, Double-Stranded/chemistry , RNA, Small Interfering/analysis , Sequence Deletion
2.
Cancer Lett ; 262(1): 37-47, 2008 Apr 08.
Article in English | MEDLINE | ID: mdl-18191020

ABSTRACT

Ashwagandha, also called as "Queen of Ayurveda" and "Indian ginseng", is a commonly used plant in Indian traditional medicine, Ayurveda. Its roots have been used as herb remedy to treat a variety of ailments and to promote general wellness. However, scientific evidence to its effects is limited to only a small number of studies. We had previously identified anti-cancer activity in the leaf extract (i-Extract) of Ashwagandha and demonstrated withanone as a cancer inhibitory factor (i-Factor). In the present study, we fractionated the i-Extract to its components by silica gel column chromatography and subjected them to cell based activity analyses. We found that the cancer inhibitory leaf extract (i-Extract) has, at least, seven components that could cause cancer cell killing; i-Factor showed the highest selectivity for cancer cells and i-Factor rich Ashwagandha leaf powder was non-toxic and anti-tumorigenic in mice assays. We undertook a gene silencing and pathway analysis approach and found that i-Extract and its components kill cancer cells by at least five different pathways, viz. p53 signaling, GM-CFS signaling, death receptor signaling, apoptosis signaling and G2-M DNA damage regulation pathway. p53 signaling was most common. Visual analysis of p53 and mortalin staining pattern further revealed that i-Extract, fraction F1, fraction F4 and i-Factor caused an abrogation of mortalin-p53 interactions and reactivation of p53 function while the fractions F2, F3, F5 work through other mechanisms.


Subject(s)
Antineoplastic Agents/pharmacology , Neoplasms/drug therapy , Phytotherapy , Plant Extracts/pharmacology , Plant Leaves , Withania , Animals , Cell Cycle , Cell Line, Tumor , Genes, Plant , Genes, p53 , Humans , Medicine, Ayurvedic , Mice , Signal Transduction/drug effects
3.
Clin Cancer Res ; 13(7): 2298-306, 2007 Apr 01.
Article in English | MEDLINE | ID: mdl-17404115

ABSTRACT

PURPOSE: Ashwagandha is regarded as a wonder shrub of India and is commonly used in Ayurvedic medicine and health tonics that claim its variety of health-promoting effects. Surprisingly, these claims are not well supported by adequate studies, and the molecular mechanisms of its action remain largely unexplored to date. We undertook a study to identify and characterize the antitumor activity of the leaf extract of ashwagandha. EXPERIMENTAL DESIGN: Selective tumor-inhibitory activity of the leaf extract (i-Extract) was identified by in vivo tumor formation assays in nude mice and by in vitro growth assays of normal and human transformed cells. To investigate the cellular targets of i-Extract, we adopted a gene silencing approach using a selected small hairpin RNA library and found that p53 is required for the killing activity of i-Extract. RESULTS: By molecular analysis of p53 function in normal and a variety of tumor cells, we found that it is selectively activated in tumor cells, causing either their growth arrest or apoptosis. By fractionation, purification, and structural analysis of the i-Extract constituents, we have identified its p53-activating tumor-inhibiting factor as with a none. CONCLUSION: We provide the first molecular evidence that the leaf extract of ashwagandha selectively kills tumor cells and, thus, is a natural source for safe anticancer medicine.


Subject(s)
Antineoplastic Agents/pharmacology , Neoplasms, Experimental/drug therapy , Phytotherapy , Plant Extracts/pharmacology , Tumor Suppressor Protein p53/drug effects , Withania/chemistry , Animals , Blotting, Western , Cell Line, Tumor , Chromatography, High Pressure Liquid , Female , Immunoprecipitation , Mice , Mice, Nude , RNA, Small Interfering
4.
J Immunol ; 176(9): 5167-71, 2006 May 01.
Article in English | MEDLINE | ID: mdl-16621980

ABSTRACT

Engagement of the FcepsilonRI expressed on mast cells induces the production of phosphatidylinositol 3, 4, 5-trisphosphate by PI3K, which is essential for the functions of the cells. PTEN (phosphatase and tensin homologue deleted on chromosome ten) directly opposes PI3K by dephosphorylating phosphatidylinositol 3, 4, 5-trisphosphate at the 3' position. In this work we used a lentivirus-mediated short hairpin RNA gene knockdown method to study the role of PTEN in CD34(+) peripheral blood-derived human mast cells. Loss of PTEN caused constitutive phosphorylation of Akt, p38 MAPK, and JNK, as well as cytokine production and enhancement in cell survival, but not degranulation. FcepsilonRI engagement of PTEN-deficient cells augmented signaling downstream of Src kinases and increased calcium flux, degranulation, and further enhanced cytokine production. PTEN-deficient cells, but not control cells, were resistant to inhibition of cytokine production by wortmannin, a PI3K inhibitor. The findings demonstrate that PTEN functions as a key regulator of mast cell homeostasis and FcepsilonRI-responsiveness.


Subject(s)
Cytokines/metabolism , Lentivirus/chemistry , Lentivirus/genetics , Mast Cells/cytology , Mast Cells/metabolism , PTEN Phosphohydrolase/metabolism , Signal Transduction , Calcium/metabolism , Cell Line , Cell Survival , Humans , Mitogen-Activated Protein Kinases/metabolism , Nucleic Acid Conformation , PTEN Phosphohydrolase/chemistry , PTEN Phosphohydrolase/genetics , Phosphatidylinositol Phosphates/metabolism , Phospholipase C gamma/metabolism , Phosphorylation , Proto-Oncogene Proteins c-akt/metabolism , RNA Interference , Receptors, IgE/metabolism
5.
Nucleic Acids Symp Ser (Oxf) ; (49): 91-2, 2005.
Article in English | MEDLINE | ID: mdl-17150648

ABSTRACT

Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we have created libraries of both ribozymes and short hairpin RNAs (shRNAs). The introduction of a library of active ribozymes or shRNAs into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.


Subject(s)
Combinatorial Chemistry Techniques , RNA Interference , RNA, Catalytic/chemistry , RNA, Untranslated/chemistry , Animals , Antineoplastic Agents, Phytogenic/pharmacology , Humans , Mice , Phenotype
6.
J Am Chem Soc ; 126(47): 15504-11, 2004 Dec 01.
Article in English | MEDLINE | ID: mdl-15563179

ABSTRACT

In the identification of a metal-binding site within enzymes, kinetic analyses based on thio-effects and Cd(2+)-rescues are widely used. In those analyses, kinetic studies using a phosphorothioate have been discussed on the premise that the substitution by a sulfur atom does not change the conformation of a ribozyme. However, our present NMR structural analysis demonstrates the change of the conformation at the metal-binding site by Rp-sulfur but not by Sp-sulfur substitution and warns against incautious interpretations of thio-effects and rescue phenomena in kinetic studies using a phosphorothioate. Our analysis further demonstrates that, in solution, a Cd(2+) ion can interact with an Rp-phosphorothioate (in support of the controversial McKay's structure, Nature 1994, 372, 68-74) and with an Sp-phosphorothioate (in support of the controversial Scott's structure, Cell 1995, 81, 991-1002) at the metal-binding A9/G10.1 site and that, in the former case, the bound Cd(2+) ion can return the ribozyme to an active conformation and rescue its enzymatic activity.


Subject(s)
Cadmium/chemistry , Oxygen/chemistry , RNA, Catalytic/chemistry , Binding Sites , Cadmium/metabolism , Nuclear Magnetic Resonance, Biomolecular/methods , Nucleic Acid Conformation , Oxygen/metabolism , RNA, Catalytic/metabolism , Sulfur/chemistry , Sulfur/metabolism
7.
J Am Chem Soc ; 126(40): 12856-64, 2004 Oct 13.
Article in English | MEDLINE | ID: mdl-15469282

ABSTRACT

The hammerhead ribozyme reaction is more complex than might have been expected, perhaps because of the flexibility of RNA, which would have enhanced the potential of RNA during evolution of and in the RNA world. Divalent Mg(2+) ions can increase the rate of the ribozyme-catalyzed reaction by approximately 10(9)-fold as compared to the background rate under standard conditions. However, the role of Mg(2+) ions is controversial since the reaction can proceed in the presence of high concentrations of monovalent ions, such as Li(+), Na(+), and NH(4)(+) ions, in the absence of divalent ions. We thus carried out ribozyme reactions under various conditions, and we obtained parameters that explain the experimental data. On the basis of the analysis, we propose a new pathway in the hammerhead ribozyme reaction in which divalent metal ions and monovalent ions act cooperatively.


Subject(s)
Lithium/chemistry , Magnesium/chemistry , RNA, Catalytic/chemistry , Base Sequence , Cations , Kinetics , Lithium/metabolism , Magnesium/metabolism , Molecular Sequence Data , Nucleic Acid Conformation , RNA, Catalytic/metabolism
8.
J Am Chem Soc ; 126(39): 12291-7, 2004 Oct 06.
Article in English | MEDLINE | ID: mdl-15453762

ABSTRACT

Hammerhead ribozymes (Rz) have catalytically important tandem G:A pairs in the core region, and we recently demonstrated that the P9-G10.1 motif (a sheared-type G:A pair with a guanine residue on the 3' side of the adenine residue) with several flanking base pairs is sufficient for capture of divalent cations, such as Mg(2+) and Cd(2+) ions that are important to maintain full activities (Tanaka et al. J. Am. Chem. Soc. 2002, 124, 4595-4601; Tanaka et al. J. Am. Chem. Soc. 2004, 126, 744-752). We also found that mutant hammerhead ribozymes that have an additional G residue inserted between A9 and G10.1 residues (the metal-binding P9-G10.1 motif) have significant catalytic activities. In this study, we demonstrate that the hammerhead ribozymes are capable of maintaining the catalytically competent structure even when the tandem, sheared-type G:A pairs were perturbed by an insertion of an additional nucleotide, whereas the chirality of the phosphorothioate at the P9 position significantly influenced the enzymatic activity for both the natural and G-inserted ribozymes.


Subject(s)
Cadmium/metabolism , Magnesium/metabolism , RNA, Catalytic/metabolism , Adenine/chemistry , Adenine/metabolism , Base Pairing , Binding Sites , Cadmium/chemistry , Cations, Divalent , Guanine/chemistry , Guanine/metabolism , Kinetics , Magnesium/chemistry , Mutagenesis, Insertional , Nucleic Acid Conformation , RNA, Catalytic/chemistry , RNA, Catalytic/genetics
9.
Nucleic Acids Res ; 32(14): 4217-23, 2004.
Article in English | MEDLINE | ID: mdl-15302920

ABSTRACT

Available evidence suggests that Mg2+ ions are involved in reactions catalyzed by hammerhead ribozymes. However, the activity in the presence of exclusively monovalent ions led us to question whether divalent metal ions really function as catalysts when they are present. We investigated ribozyme activity in the presence of high levels of Mg2+ ions and the effects of Li+ ions in promoting ribozyme activity. We found that catalytic activity increased linearly with increasing concentrations of Mg2+ ions and did not reach a plateau value even at 1 M Mg2+ ions. Furthermore, this dependence on Mg2+ ions was observed in the presence of a high concentration of Li+ ions. These results indicate that the Mg2+ ion is a very effective cofactor but that the affinity of the ribozyme for a specific Mg2+ ion is very low. Moreover, cleavage by the ribozyme in the presence of both Li+ and Mg2+ ions was more effective than expected, suggesting the existence of a new reaction pathway-a cooperative pathway-in the presence of these multiple ions, and the possibility that a Mg2+ ion with weak affinity for the ribozyme is likely to function in structural support and/or act as a catalyst.


Subject(s)
Magnesium/pharmacology , RNA, Catalytic/metabolism , Catalysis , Lithium/pharmacology , Magnesium/metabolism , Magnesium/physiology , RNA/chemistry , RNA/metabolism , RNA, Catalytic/drug effects
10.
Nucleic Acids Res ; 32(15): 4618-29, 2004.
Article in English | MEDLINE | ID: mdl-15333694

ABSTRACT

During an analysis of DNA enzymes by gel electrophoresis, we found that some DNA enzymes can adopt more than one conformation. The DNA enzyme Dz31 that formed more than one conformer contained a stretch of G residues. Further investigations, involving kinetic analysis and measurements of circular dichroism, indicated that this DNA enzyme and its derivatives formed G-quadruplexes. Moreover, we found that some derivative oligomers were capable of forming dimeric G-quadruplexes. We also compared the catalytic activities of Dz31 and its mutant derivatives. The present findings suggest that DNA enzymes with five or more continuous G residues are less favorable than those without G5 in the association step in the enzymatic reaction and, thus, the choice of targets that contain a continuous stretch of C residues downstream of the cleavage site should be avoided. In addition, we found that negative charge-charge repulsion disrupted the dimerization of G-quadruplexes when a phosphate group was added directly to the 5'-terminal G of oligomers with continuous guanosine residues. In the case of 5'-phosphorylated G5CTA, direct attachment of a phosphate group to the continuous G5 sequence inhibited dimerization of G-quadruplexes, at least during electrophoresis on a denaturing gel.


Subject(s)
DNA, Catalytic/chemistry , DNA, Catalytic/metabolism , DNA/chemistry , DNA/metabolism , Guanosine/metabolism , Circular Dichroism , Dimerization , Electrophoresis, Polyacrylamide Gel , G-Quadruplexes , Guanosine/chemistry , Nucleic Acid Conformation , Phosphates , Phosphorylation
12.
J Am Chem Soc ; 126(3): 744-52, 2004 Jan 28.
Article in English | MEDLINE | ID: mdl-14733548

ABSTRACT

We have studied the interaction between metal ions and the metal ion-binding motif in hammerhead ribozymes, as well as the functions of the metal ion at the motif, with heteronuclear NMR spectroscopy. In this study, we employed model RNA systems which mimic the metal ion-binding motif and the altered motif. In Co(NH3)6(III) titrations, we observed large 1H and 31P chemical shift perturbations for the motif and found that outer-sphere complexation of Co(NH3)6(III) is possible for this motif. From the reinvestigation of our previous 15N chemical shift data for Cd(II) binding, in comparison with those of organometallic compounds, we conclude that Cd(II) can form an inner-sphere complex with the nucleobase in the motif. Therefore, the A9/G10.1 site was found to accept both inner-sphere and outer-sphere complexations. The Mg(II) titration for a slightly different motif from the A9/G10.1 site (G10.1-C11.1 to A10.1-U11.1) revealed that its affinity to Mg(II) was drastically reduced, although the ribozyme with this altered motif is known to retain enzymatic activities. This observation suggests that the metal ion at these motifs is not a catalytic center of hammerhead ribozymes.


Subject(s)
Metals/chemistry , Metals/metabolism , RNA, Catalytic/chemistry , RNA, Catalytic/metabolism , Cations , Nuclear Magnetic Resonance, Biomolecular/methods , Nucleic Acid Conformation
13.
Nucleic Acids Symp Ser (Oxf) ; (48): 133-4, 2004.
Article in English | MEDLINE | ID: mdl-17150514

ABSTRACT

The cyclic guanine tetramer (G-quartet) formation was theoretically studied. Total hydrogen bond energy in a G-quartet was large (-70.89 kcal/mol). though the hydrogen bond energy in Hoogsteen type guanine dimer was small (-11.26 kcal/mol). A large attractive interaction (-57.84 kcal/mol) was observed for stacking interaction. The hydration effect should be considered to explain the selectivity of metal incorporation into the G-quartet.


Subject(s)
Guanine/chemistry , Models, Theoretical , Nucleic Acid Conformation , Hydrogen Bonding , Metals/chemistry , Thermodynamics
14.
Magnes Res ; 16(3): 210-7, 2003 Sep.
Article in English | MEDLINE | ID: mdl-14596326

ABSTRACT

Analysis, based on kinetic solvent isotope effects, demonstrated that no proton transfer occurs in reactions catalyzed by a 32-mer hammerhead ribozyme (R32) in the presence of magnesium ions, whereas proton transfer occurs in reactions catalyzed by R32 in the presence of high concentrations of monovalent NH4+ ions without metal ions, demonstrating that the detailed mechanism of action of the hammerhead ribozyme might change depending on the environment. Importantly, when the concentration of magnesium ions was gradually increased from 1 mM to up to 800 mM, the R32 ribozyme activity increased linearly without reaching a plateau value. This phenomenon can be explained by a model in which a catalytic magnesium ion with a very low affinity (dissociation constant, Kd > 800 mM) exists and/or the predominant inactive complex converts to a minor active complex before the cleavage reaction.


Subject(s)
Magnesium/pharmacology , RNA, Catalytic/metabolism , Base Sequence , Catalysis/drug effects , Dose-Response Relationship, Drug , Molecular Sequence Data , Protein Structure, Secondary
15.
Nucleic Acids Res Suppl ; (3): 191-2, 2003.
Article in English | MEDLINE | ID: mdl-14510445

ABSTRACT

A linear, covalently-closed, dumbbell-shaped DNA vector including a transcription unit is known to have both biological stability and safety and is expected to be useful for gene therapy. We established an easy, quick, and large preparative synthetic method of modified- and unmodified-dumbbell DNA using an intramolecular cyclization at the DNA termini.


Subject(s)
DNA/chemical synthesis , Polymerase Chain Reaction/methods , DNA/chemistry , Electrophoresis, Polyacrylamide Gel , In Vitro Techniques
16.
J Am Chem Soc ; 124(28): 8230-6, 2002 Jul 17.
Article in English | MEDLINE | ID: mdl-12105900

ABSTRACT

It has been generally accepted, on the basis of kinetic studies with phosphorothioate-containing substrates and analyses by NMR spectroscopy, that a divalent metal ion interacts directly with the pro-Rp oxygen at the cleavage site in reactions catalyzed by hammerhead ribozymes. However, results of our recent kinetic studies (Zhou, D.-M.; Kumar, P. K. R.; Zhang. L. H.; Taira, K. J. Am. Chem. Soc. 1996, 118, 8969-8970. Yoshinari, K.; Taira, K. Nucleic Acids Res. 2000, 28, 1730-1742) demonstrated that a Cd(2+) ion does not interact with the sulfur atom at the Rp position of the scissile phosphate (P1.1) in the ground state or in the transition state. Therefore, in the present study, we attempted to determine by (31)P NMR spectroscopy whether a Cd(2+) ion binds to the P1.1 phosphorothioate at the cleavage site in solution. In the case of the R32-S11S (ribozyme-substrate) complex, neither the Rp- nor the Sp-phosphorothioate signal from the S11S substrate at the cleavage site was perturbed (the change was less than 0.1 ppm) upon the addition of Cd(2+) ions (19 equiv) at pH 5.9 and 8.5. By contrast, we detected the significant perturbation of the P9 phosphorothioate signal from another known metal-binding site (the A9/G10.1 metal-binding motif). The Rp-phosphorothioate signal from A9/G10.1 was shifted by about 10 ppm in the higher field direction upon the addition of Cd(2+) ions. These observations support the results of our kinetic analysis and indicate that a Cd(2+) ion interacts with the sulfur atom of the phosphorothioate at the A9/G10.1 site (P9) but that a Cd(2+) ion does not interact with the sulfur atom at the Rp- or at the Sp-position of the scissile phosphate (P1.1) in the ground state.


Subject(s)
Cadmium/metabolism , RNA, Catalytic/metabolism , Thionucleotides/metabolism , Base Sequence , Binding Sites , Cadmium/chemistry , Nuclear Magnetic Resonance, Biomolecular/methods , Nucleic Acid Conformation , Phosphorus , RNA, Catalytic/chemistry , Thionucleotides/chemistry
17.
Nucleic Acids Res ; 30(11): 2374-82, 2002 Jun 01.
Article in English | MEDLINE | ID: mdl-12034824

ABSTRACT

The hammerhead ribozyme is generally accepted as a well characterized metalloenzyme. However, the precise nature of the interactions of the RNA with metal ions remains to be fully defined. Examination of metal ion-catalyzed hammerhead reactions at limited concentrations of metal ions is useful for evaluation of the role of metal ions, as demonstrated in this study. At concentrations of Mn2+ ions from 0.3 to 3 mM, addition of the ribozyme to the reaction mixture under single-turnover conditions enhances the reaction with the product reaching a fixed maximum level. Further addition of the ribozyme inhibits the reaction, demonstrating that a certain number of divalent metal ions is required for proper folding and also for catalysis. At extremely high concentrations, monovalent ions, such as Na+ ions, can also serve as cofactors in hammerhead ribozyme-catalyzed reactions. However, the catalytic efficiency of monovalent ions is extremely low and, thus, high concentrations are required. Furthermore, addition of monovalent ions to divalent metal ion-catalyzed hammerhead reactions inhibits the divalent metal ion-catalyzed reactions, suggesting that the more desirable divalent metal ion-ribozyme complexes are converted to less desirable monovalent metal ion-ribozyme complexes via removal of divalent metal ions, which serve as a structural support in the ribozyme complex. Even though two channels appear to exist, namely an efficient divalent metal ion-catalyzed channel and an inefficient monovalent metal ion-catalyzed channel, it is clear that, under physiological conditions, hammerhead ribozymes are metalloenzymes that act via the significantly more efficient divalent metal ion-dependent channel. Moreover, the observed kinetic data are consistent with Lilley's and DeRose's two-phase folding model that was based on ground state structure analyses.


Subject(s)
Cations, Divalent/metabolism , Cations, Monovalent/metabolism , Metals/metabolism , RNA, Catalytic/metabolism , Base Sequence , Catalysis/drug effects , Cations, Divalent/pharmacology , Cations, Monovalent/pharmacology , Electron Spin Resonance Spectroscopy , Energy Transfer , Fluorescence , Kinetics , Magnesium/metabolism , Magnesium/pharmacology , Manganese/metabolism , Manganese/pharmacology , Metals/pharmacology , Nucleic Acid Conformation , Protons , RNA, Catalytic/chemistry , RNA, Catalytic/genetics , Sodium/metabolism , Sodium/pharmacology , Titrimetry
18.
Nucleic Acids Res ; 30(11): 2383-9, 2002 Jun 01.
Article in English | MEDLINE | ID: mdl-12034825

ABSTRACT

Exploitation of ribozymes in a practical setting requires high catalytic activity and strong specificity. The hammerhead ribozyme R32 has considerable potential in this regard since it has very high catalytic activity. In this study, we have examined how R32 recognizes and cleaves a specific substrate, focusing on the mechanism behind the specificity. Comparing rates of cleavage of a substrate in a mixture that included the correct substrate and various substrates with point mutations, we found that R32 cleaved the correct substrate specifically and at a high rate. To clarify the source of this strong specificity, we quantified the weak interactions between R32 and various truncated substrates, using truncated substrates as competitive inhibitors since they were not readily cleaved during kinetic measurements of cleavage of the correct substrate, S11. We found that the strong specificity of the cleavage reaction was due to a closed form of R32 with a hairpin structure. The self-complementary structure within R32 enabled the ribozyme to discriminate between the correct substrate and a mismatched substrate. Since this hairpin motif did not increase the Km (it did not inhibit the binding interaction) or decrease the kcat (it did not decrease the cleavage rate), this kind of hairpin structure might be useful for the design of new ribozymes with strong specificity and high activity.


Subject(s)
RNA, Catalytic/chemistry , RNA, Catalytic/metabolism , Base Pair Mismatch/genetics , Base Sequence , Binding, Competitive , Catalysis , Genetic Engineering , Hydrolysis , Kinetics , Mutation/genetics , Nucleic Acid Conformation , RNA, Catalytic/genetics , Substrate Specificity , Thermodynamics
19.
J Am Chem Soc ; 124(15): 3850-2, 2002 Apr 17.
Article in English | MEDLINE | ID: mdl-11942820

ABSTRACT

Hammerhead ribozymes have been considered to be metalloenzymes. However, this proposal was recently questioned by the finding that the reaction proceeds in the presence of high concentrations of monovalent ions such as NH(4)(+) ions and in the absence of any divalent metal ions. Our present analysis based on solvent isotope effects indicates that (1) a proton transfer(s) occurs only in the NH(4)(+)-mediated reaction but not in metal-ion-mediated reactions such as Mg(2+)- and Li(+)-mediated reactions, (2) the catalyst that stabilizes the 5' leaving group in the NH(4)(+)-mediated reaction is different from that in the metal-ion-mediated HH ribozyme reactions, (3) an NH(4)(+) ion seems to act as a general acid catalyst, and (4) a nucleobase alone should not be the catalyst.


Subject(s)
Quaternary Ammonium Compounds/chemistry , RNA, Catalytic/chemistry , Base Sequence , Catalysis , Cations , Deuterium , Kinetics , Lithium/chemistry , Lithium/metabolism , Magnesium/chemistry , Magnesium/metabolism , Nucleic Acid Conformation , Protons , RNA, Catalytic/metabolism
20.
Nucleic Acids Res Suppl ; (2): 273-4, 2002.
Article in English | MEDLINE | ID: mdl-12903210

ABSTRACT

Hammerhead ribozymes have been considered to be divalent-metalloenzymes. However, this was recently questioned by the finding that the reaction can proceed without any divalent metal ions in the presence of high concentrations of monovalent ions such as NH4+/Li+ ions. Thus, one might think divalent metal ions are not involved in chemical step in the catalytic mechanism. To investigate the involvement of the monovalent ions, we analyzed the deuterium solvent isotope effects in the reactions. Our present analysis indicates a proton transfer(s) occurs only in the NH(4+)-mediated reaction, not in the Li(+)-mediated. Most simple interpretation is that NH4+ works as a general acid catalyst and Li+ as Lewis acid catalyst. This suggests hammerhead ribozymes can change catalyst upon their surrounding conditions.


Subject(s)
Lithium/metabolism , Quaternary Ammonium Compounds/metabolism , RNA, Catalytic/metabolism , Base Sequence , Cations, Monovalent , Isotopes , Kinetics , Nucleic Acid Conformation , RNA, Catalytic/chemistry
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