Iridoid glucosides from the leaves and stems of Duranta erecta.

From the leaves of Duranta erecta, four new iridoid glucosides, duranterectosides A, B, C and D, were isolated along with durantosides I and II, lamiide, lamiidoside and verbascoside. Duranterectoside A was also isolated from the stems together with durantosides I, II and III, and lamiidoside. The structures of the new compounds were elucidated based on the spectroscopic evidence.

[Serotypes of enteroadherent Escherichia coli exhibiting diffuse pattern of adherence isolated from diarrheal patients and healthy controls in Brazil, Myanmar and Japan].

We tried to detect enteroadherent Escherichia coli exhibiting a diffuse pattern of adherence to HeLa cells from stock strains derived from patients with or without diarrhea in Brazil, Myanmar and Japan (Osaka). Enteroadherent E. coli was found from 16 (23 strains) out of 126 (384 strains) in diarrheal infant cases (12.7%), 26 (29 strains) out of 126 (348 strains) in healthy control cases (20.6%) in Brazil, from 15 (18 strains) out of 221 (542 strains) in diarrheal infant cases (6.8%), 4 (4 strains) out of 87 (212 strains) in healthy control cases (4.6%) in Myanmar. In Japan (Osaka), enteroadherent E. coli was detected from 7 (7 strains) out of 123 (198 strains) in diarrheal cases (5.7%). These results show that enteroadherent E. coli (diffuse) may not be associated with diarrhea. Forty-four (62.9%) out of 70 strains belonged to 19 O serogroups or 21 O:H serotypes. All strains were H antigen serotypable, and 26 strains were found to be nonmotile. The predominant O:H serotypes were O11:H15, O11:H-, O20:H34, O21:H5, O89:H-, O99:H33 and O154:H45. Only one strain belonged to enteropathogenic E. coli serogroup O127.

[Incidence and prevalence of serotypes of enteroaggregative Escherichia coli from diarrheal patients in Brazil, Myanmar and Japan].

By using HeLa cells, we tried to detect enteroaggregative Escherichia coli from stock strains derived from infants with or without diarrhea in Brazil and Myanmer, and from diarrheal patients in Osaka, Japan. Enteroaggregative E. coli was detected from 7.9% in diarrheal cases, 5.6% in control cases in Brazil and from 5.4% in diarrheal cases, 3.4% in control cases in Myanmar. A total of 38 strains that showed aggregative adherence to HeLa cells was examined for colony hybridization with the 32P-labeled EAggEC probe. Thirty-four strains hybridized with the EAggEC probe, while 4 strains did not hybridize with the probe, suggesting an existence of another aggregative factor. Seventeen of the 38 strains were O-antigen serotypeable. Eight strains belonged to serogroups O44, O86, O111, O125 and O126, which have been considered to be enteropathogenic E. coli serogroups. Most of the 38 strains were H-antigen serotypeable.

[Enteroadherent Escherichia coli exhibiting localized pattern of adherence among infants with diarrhoea in Brazil--incidence and prevalence of serotypes].

The incidence of enteroadherent Escheridhia coli exhibiting localized adherence to HeLa cells was investigated using the EAF probe (Nataro et al., J. Infect. Dis., 152:560-563, 1985) among 126 infants below 3 years of age along with 126 age-matched healthy controls in Brazil. The EAF probe proved to be sensitive and specific in detection of enteroadherent E. coli. EAF-probe positive E. coli was isolated from 23.0% of the infants with acute diarrhoea while the corresponding rate of isolation from healthy controls was 11.9%. EAF-probe positive E. coli strains belonging to the classical enteropathogenic E. coli (EPEC) serogroups were more often associated with diarrheal cases (18.3%) than with strains isolated from control healthy infants (5.6%). The predominant EAF-probe positive E. coli serotypes were O55:H-, O111:H2 and O119:H6. These serotypes, especially O111:H2, were mainly isolated from cases with diarrhoea suggesting a strong causal association. Among the EAF positive non-EPEC serotypes, the most prevalent serotype was O88:H25 and this represents a, hitherto, unrecognized diarrheagenic E. coli serotype.

Localized, diffuse, and aggregative-adhering Escherichia coli from infants with acute diarrhea and matched-controls.

Of 126 infants under 2 years, enrolled in a study on the etiology of acute diarrhea in Recife, Brazil, we selected 37 from whom no recognized enteropathogens, except classic enteropathogenic Escherichia coli, were identified. For comparison, we also examined 37 matched-control infants without diarrhea seen at the same hospital setting. This paper had the purpose to determine the prevalence of localized, diffuse, and aggregative-adhering E. coli strains in both groups. Three to five fecal E. coli colonies, of each case and control, were tested individually for adherence to HeLa cells by using the one step 3-h incubation assay. Strains of E. coli showing localized adherence were found significantly more often in patients (37.8%) than in controls (13.5%), p < 0.02, and they were practically confined to EPEC serovars 055:H-, 0111:H2, and 119:H6. In contrast, E. coli isolates exhibiting the diffuse or aggregative patterns of adherence were restricted to non-EPEC serogroups and were more frequently encountered among controls.

Microbiologia da diarréia aguda endêmica em crianças do Recife / Microbiology of endemic acute diarrhoea in children of Recife

Entre fevereiro e junho de 1989, investigaram-se 126 crianças diarréicas e 126 controles, pareados pela idade, para a detecçäo de bactérias, vírus e parasitos enteropatogênicos. Identificaram-se um ou mais patógenos em 81,8% dos pacientes e em 61,9% dos controles. Escherichia coli enteropatogênica(EPEC) foi o patógeno mais frequentemente identificado. EPEC mostrando aderência localizada, E.coli enteroaderente agregativa, Shigella, Rotavírus e Cryptosporidium foram associados com os casos significativamente. Por outra parte, EPEC näo aderente, E.Coli exibindo aderência difusa, E.coli enterotoxigênica (ETEC) produtora de LT, Salmonella, Campylobacter, Adenovírus, Giardia lamblia e Endamoeba histolytica näo mostraram diferenças significativas entre casos e controles. ETEC produtoras de ST,E.coli enteroinvasiva e enterohemorrágica, Aeromonas, Pleisaomonas e Vibrio foram isolados em taxas muito baixas para permitir avaliar seus papéis na doenças diarréica, no Recife

Emergence of a restricted bioserovar of Vibrio parahaemolyticus as the predominant cause of Vibrio-associated gastroenteritis on the West Coast of the United States and Mexico.

Examination of 45 human fecal isolates of Vibrio parahaemolyticus revealed the emergence of an unusual bioserovar (O4:K12, urease positive) associated with cases of gastroenteritis which appear to be domestically acquired on the West Coast of the United States and Mexico.

Enzyme-linked immunosorbent assay to measure antibodies to purified heat-labile enterotoxins from human and porcine strains of Escherichia coli and to cholera toxin: application in serodiagnosis and seroepidemiology.

Serum immunoglobulin G antibodies to purified heat-labile enterotoxin (LT) from human (LTh) and porcine (LTp) Escherichia coli strains and cholera enterotoxin (CT) were measured by an enzyme-linked immunosorbent assay. Sera from patients with LTh E. coli infection showed a prominent response with LTh, an intermediate response with LTp, and a meager response with CT. Of 47 persons with clinical LTh-producing E. coli (herein shortened to LTh E. coli) infections, significant rises in antitoxin were detected against LTh in 36 (77%), against LTp in 30 (64%), and against CT in only 13 (28%) patients; seroconversions also occurred in 11 of 14 (79%) patients with subclinical LTh E. coli infections. In North Americans with experimental LTh E. coli infection, anti-Lth did not remain at high levels for more than 3 months. Persons with cholera manifested antitoxin responses that were similarly potent against all three toxin antigens; in fact, net optical density values were often slightly higher against LTh than against CT. The ratio of CT/LTh ELISA net optical density in convalescent sera proved to be a sensitive means to differentiate LT E. coli from cholera infection. All 11 cholera patients tested had CT/LTh ratios of greater than 0.70, whereas in only 1 of 47 LTh E. coli infections did the ratio exceed that value (it was 0.71) (P less than 0.0000000001). In single serum specimens, a net optical density of greater than or equal to 0.30 against LTh was shown to be a useful cutoff in screening sera for recent LTh E. coli or past cholera infection. The CT/LTh ratio was then used to differentiate definitively. Sera from healthy 3- to 5-year -olds and 15- to 19-year-olds in Maryland, Chile, and Bangladesh were tested against LTh and CT. The serological results fit known epidemiological observations. (i) LTh infections are rare in the United States (only 2 of 60 sera had LTh net optical density values of >/= 0.30. (ii) In contrast, evidence of recent LTh E. coli infections was very common in Chilean (69%) and Bangladeshi (57%) 3- to 5-year-olds and not uncommon in 15- to 19-year-olds (38 and 31%, respectively) in those countries. (iii) Only Bangladeshi sera showed serological evidence of cholera infections (CT/LTh ratios of > 0.70). The immunoglobulin G enzyme-linked immunosorbent assay measuring antibodies to purified LTh and CT represents a practical and effective tool for the serological study of LTh E. coli and cholera diarrheal infections.