ABSTRACT
This study examines the factorial invariance of the Optimization in Primary and Secondary Control (OPS) scale and its associations with subjective well-being among older couples in Japan and the US. To this end, 200 older couples in Japan and 220 in the US were recruited through paid vendors and completed the questionnaire online. Couples were eligible if husbands were 70 years or older and wives were 60 years or older. A six-factor model, in which Compensatory Primary Control was subdivided into two factors, fit the data best; its factorial invariance was confirmed among the four subsamples. Compensatory Secondary Control was more strongly associated with subjective well-being in American couples than in Japanese couples, although the associations between well-being and the other five OPS factors were similar in the two countries. Future research on this six-factor model will be able to examine how these control strategies function in different cultures.
ABSTRACT
Constitutive androstane receptor (CAR) is a transcription factor regulating the expression of several genes related to drug metabolism. CAR expression was elevated in human HepG2 and SW480 cells by serum starvation. From reporter gene assays, mutagenesis, RNA interference, and chromatin immunoprecipitation assays, we identified the serum response element at -142/-139 in the CAR gene transactivated by Elk-1. Whereas treatment with U0126 (ERK inhibitor) enhanced CAR expression, SP600125 (stress-activated protein kinase inhibitor, SAPK) suppressed the phosphorylation of Elk-1 caused by serum-starvation stress and the elevation of CAR mRNA, suggesting that CAR expression may be mediated by phosphorylated Elk-1 via the SAPK signaling pathway.
Subject(s)
Receptors, Cytoplasmic and Nuclear/metabolism , Stress, Physiological , Transcription Factors/metabolism , Up-Regulation , ets-Domain Protein Elk-1/metabolism , 5' Flanking Region/genetics , Anthracenes/pharmacology , Base Sequence , Cell Line, Tumor , Constitutive Androstane Receptor , Culture Media, Serum-Free , Humans , Molecular Sequence Data , Phosphorylation/drug effects , Protein Binding , RNA, Messenger/genetics , Receptors, Cytoplasmic and Nuclear/genetics , Signal Transduction , Transcription Factors/genetics , Transcriptional Activation/genetics , ets-Domain Protein Elk-1/geneticsABSTRACT
Constitutive androstane receptor (CAR) is a transcription factor to regulate the expression of several genes related to drug-metabolism. Here, we demonstrate that CAR protein accumulates during G1 in human SW480 and HepG2 cells. After the G1/S phase transition, CAR protein levels decreased, and CAR was hardly detected in cells by the late M phase. CAR expression in both cell lines was suppressed by RNA interference-mediated suppression of CDK4. Depletion of CAR by RNA interference in both cells and by hepatocyte growth factor treatment in HepG2 cells resulted in decreased MDM2 expression that led to p21 upregulation and repression of HepG2 cell growth. Thus, our results demonstrate that CAR expression is an early G1 event regulated by CDK4 that contributes to MDM2 expression; these findings suggest that CAR may influence the expression of genes involved in not only the metabolism of endogenous and exogenous substances but also in the cell proliferation.
