Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 35
Filter
1.
J Orthop Surg (Hong Kong) ; 25(1): 2309499016684322, 2017 01.
Article in English | MEDLINE | ID: mdl-28142352

ABSTRACT

The purpose of this study was to evaluate factors that correlated with unsatisfactory short- and long-term outcome in patients who sustained unstable pelvic ring fracture. The study subjects of this study were those of type B and C pelvic ring fractures (82 patients; mean age 54 years). Age, gender, associated injuries, fracture type, Injury Severity Score rating and treatment methods were assessed, and Majeed score for functional outcome and radiographic studies at 1 year after injury (short-term) and at final follow-up (long-term), with mean follow-up of 98 months were analyzed. Significant univariate factors ( p < 0.05) were entered in a multivariate logistic regression model to determine the independent predictors of unsatisfactory functional outcome. Univariate analysis showed that fractures of the lower extremity, nerve damage, conservative treatment, and radiological outcome correlated with unsatisfactory short-term functional outcome, while female gender, brain injury, nerve damage, conservative treatment, fracture location at the posterior portion of pelvic ring, radiological outcome, and pure sacroiliac dislocation only for type C fracture correlated with unsatisfactory long-term outcome. Multiple logistic regression analysis identified fractures of the lower extremity (odds ratio (OR): 5.364), conservative treatment (OR: 13.690), and nerve damage (OR: 21.392) as determinants of unsatisfactory short-term functional outcome and nerve damage (OR: 66.926) and poor radiological results (OR: 33.944) as determinant of long-term functional outcome. In patients with unstable pelvic ring injury, fractures of the lower extremity, conservative therapy, and nerve damage influenced short-term functional outcome, while that nerve damage and the pelvic ring displacement over 20 mm negatively affected long-term outcome.


Subject(s)
Fractures, Bone/surgery , Pelvic Bones/injuries , Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Fractures, Bone/diagnostic imaging , Humans , Injury Severity Score , Joint Dislocations/complications , Logistic Models , Male , Middle Aged , Radiography , Recovery of Function , Sacroiliac Joint/injuries , Treatment Outcome , Young Adult
2.
Springerplus ; 5: 597, 2016.
Article in English | MEDLINE | ID: mdl-27247893

ABSTRACT

BACKGROUND: Massive bone defects of the acetabulum with pelvic discontinuity are one of the major problems in revision total hip arthroplasty. Several techniques have been described for repair of acetabular defect; however, reconstruction of acetabulum with massive bone defect is still a major problem. We describe a patient who required four revision total hip arthroplasty during a 24-year period. FINDINGS: The acetabulum with pelvic discontinuity was successfully reconstructed by stabilization of the posterior column with a plate commonly used for fracture treatment, and stabilization of the anterior column by reinforcement device commonly used for acetabular reconstruction. Fixation of both acetabular columns provided significant improvement of component stability. CONCLUSIONS: In the case of pelvic discontinuity with massive acetabular bone defect, reconstruction by stabilizing both acetabular columns using reconstruction plate and KT plate is one of the better surgical options.

3.
Eur J Orthop Surg Traumatol ; 26(4): 407-13, 2016 May.
Article in English | MEDLINE | ID: mdl-27010392

ABSTRACT

The aim of the present study was to analyze the clinical and radiographic outcomes and Kaplan-Meier survivorship of patients who underwent revision surgeries of the acetabular cup that had sustained aseptic loosening. We reviewed 101 consecutive patients (120 hips; 10 men 11 hips; 91 women 109 hips; age at surgery 66 years; range 45-85) who underwent acetabular component revision surgery, at a follow-up period of 15.6 years (range 10-32). To evaluate the state of the acetabulum, acetabular bony defects were classified according to the AAOS classification based on intraoperative findings: type I (segmental deficiencies n = 24 hips), type II (cavity deficiency n = 48), type III (combined deficiency n = 46), and type IV (pelvic discontinuity n = 2). The Harris hip score improved from 42.5 ± 10.8 (mean ± SD) before surgery to 74.9 ± 14.6 points at follow-up. The survival rates of the acetabular revision surgery with cemented, cementless, and cemented cups plus reinforcement devices were 74, 66, and 82 %, respectively. The difference in the survival rate between the cemented and cementless group was marginal (p = 0.048 Gehan-Breslow-Wilcoxon, p = 0.061 log-rank), probably due to the early-stage failure cases in the cementless group. The cementless and reinforcement groups included nine early-stage failure cases. To prevent early-stage failure, we recommend the cementless cups for types I and II acetabular bone defects with adequate contact between host bone and acetabular component, and the cemented cup with or without reinforcement devices, together with restoration of bone stock by impaction or structured bone grafting, for cases lacking such contact.


Subject(s)
Acetabulum/injuries , Fractures, Bone/surgery , Acetabulum/surgery , Aged , Aged, 80 and over , Arthroplasty, Replacement, Hip/instrumentation , Arthroplasty, Replacement, Hip/methods , Bone Cements , Female , Hip Prosthesis , Humans , Male , Middle Aged , Prosthesis Failure , Reoperation/statistics & numerical data
4.
Eur J Orthop Surg Traumatol ; 23(2): 155-63, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23412446

ABSTRACT

Acetabular fracture result in fairly good outcome after the anatomic reduction in the displaced fracture fragments and damaged joint structure, but some patients will inevitably suffer from hip joint problems during their courses after the insult. We retrospectively reviewed 91 patients with acetabular fractures to investigate the causes of clinical failure and relationship among the fracture types, selected treatment options and their courses. Ninety-one patients (73 men and 18 women) with an average age of 49 years (range 18-80) at the time of injury were followed up for an average of 8.6 years (range 2-18). Judet-Letournel classification of fracture type and Matta's rating regimen of functional and radiographic patient' assessment were conducted. Conservative treatment was provided in 20 patients, in which 19 attained excellent/good, and one fair clinical results. All achieved excellent/good radiographic outcome. Surgically treated patients (n = 71) with critical dislodgement of the fracture fragment showed that 64 (90%) attained excellent/good and 7 (10%) fair/poor clinical outcomes. Sixty-three (89%) attained excellent/good and 8 (11%) fair/poor postoperative radiographic outcome. Five patients with poor radiographic outcome after surgery subsequently required total hip arthroplasty, due to the development of hip joint osteoarthritis in 3 and femoral head avascular necrosis in 2. We conclude that displacement of the joint surface should be reduced to less than 3 mm in accordance with the selection of the most appropriate surgical approach for open reduction/fixation in each fracture type; however, comminuted fracture and avascular necrosis of the femoral head may be the cause of poor clinical results.


Subject(s)
Acetabulum/injuries , Fractures, Bone/therapy , Acetabulum/diagnostic imaging , Acetabulum/surgery , Adolescent , Adult , Aged , Aged, 80 and over , Female , Fracture Fixation , Fracture Fixation, Internal , Fractures, Bone/diagnostic imaging , Fractures, Bone/surgery , Humans , Male , Middle Aged , Radiography , Retrospective Studies , Treatment Outcome , Young Adult
5.
Eur J Orthop Surg Traumatol ; 23(5): 557-64, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23412160

ABSTRACT

This report describes case series of the femoral head fractures associated with fracture-dislocation of the hip joint to evaluate the mid- and long-term outcomes and to highlight the surgical technique of fixation of the femoral head from the posterior trochanteric flip osteotomy approach. Twelve patients (6 men and 6 women) with dislocated femoral head fractures (mean age at the time of injury, 56 years; range, 23-80) were followed up for mean period of 9.7 years (range, 5-20). All dislocations were reduced within less than 6 h after the injury. The type of femoral head fracture was classified according to the Pipkin classification on radiographs and CT. Five patients were classified as type I, 2 as type II, 2 as type III, and 3 as type IV. The clinical and radiological outcomes were assessed by Thompson and Epstein's regimen. Excluding 2 patients with Pipkin type III, the outcome of 9 patients was excellent/good, and poor in 1. The latter patient sustained Pipkin type IV and developed osteoarthritis 1 year after surgery and consequently required total hip arthroplasty. We conclude that small fragment of the femoral head less than 1 cm can be removed, while larger fragments should be fixed by bioabsorbable screws or pins in all types of femoral head fractures. In Pipkin type IV fractures, surgeons should always take anatomical reduction in the acetabulum into consideration during surgery.


Subject(s)
Femur Head/injuries , Fracture Fixation, Internal/methods , Hip Dislocation/surgery , Hip Fractures/surgery , Osteotomy/methods , Acetabulum/diagnostic imaging , Acetabulum/surgery , Adult , Aged , Aged, 80 and over , Cohort Studies , Female , Follow-Up Studies , Fracture Fixation, Internal/instrumentation , Fracture Healing/physiology , Hip Dislocation/diagnostic imaging , Hip Fractures/diagnostic imaging , Humans , Intra-Articular Fractures/diagnostic imaging , Intra-Articular Fractures/surgery , Male , Middle Aged , Radiography , Range of Motion, Articular/physiology , Recovery of Function , Retrospective Studies , Risk Assessment , Time Factors , Treatment Outcome , Young Adult
6.
Artif Organs ; 37(2): 175-82, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23009086

ABSTRACT

We have designed a proximal-fitting, anterolaterally flared, arc-deposit hydroxyapatite-coated anatomical femoral stem (FMS-anatomic stem; KYOCERA Medical, Osaka, Japan) for cementless total hip arthroplasty (THA) for Japanese patients with dysplastic hip osteoarthritis, using a nonlinear three-dimensional finite element analysis simulating loading conditions. The Anatomic Fit stem was modified in the region of the arc-sprayed surface, to allow more proximal appearance of spot welds. The aim of the present study was to analyze the clinical and radiographic outcomes of patients who underwent THA using this stem. We reviewed 73 consecutive patients (79 hips; 13 men 16 hips; 60 women 63 hips; age at surgery, 57.6 years, range, 35-78) who underwent cementless THA using the Anatomic Fit stem, at a follow-up period of 7.1 years (range, 5.1-9.4). Harris Hip score improved from 40.7 ± 17.1 before surgery to 91.0 ± 5.2 points at follow-up. The 7.1-year stem survival rate was 100%. Radiographs at follow-up confirmed the stability of the femoral stems within the femoral canal in all cases, with sufficient bone ingrowth. None of the patients had subsidence of the stem exceeding 2.0 mm within the femoral canal or changes in varus or valgus position of more than 2.0°. The Anatomic Fit stem provided excellent results. The nonlinear three-dimensional finite element analysis demonstrated that the stem-bone relative motion was 10 µm at the proximal end of the stem and proximal load transfer. Our analysis confirmed reduced radiolucency around the stem, minimal subsidence, appropriate stress shielding, and promising medium-term stability within the femoral canal.


Subject(s)
Arthroplasty, Replacement, Hip/instrumentation , Computer-Aided Design , Femur/surgery , Hip Dislocation, Congenital/surgery , Hip Joint/surgery , Hip Prosthesis , Osteoarthritis, Hip/surgery , Adult , Aged , Arthroplasty, Replacement, Hip/adverse effects , Biomechanical Phenomena , Case-Control Studies , Coated Materials, Biocompatible , Durapatite , Female , Femur/diagnostic imaging , Femur/physiopathology , Finite Element Analysis , Follow-Up Studies , Hip Dislocation, Congenital/diagnostic imaging , Hip Dislocation, Congenital/physiopathology , Hip Joint/diagnostic imaging , Hip Joint/physiopathology , Humans , Japan , Male , Middle Aged , Nonlinear Dynamics , Osseointegration , Osteoarthritis, Hip/diagnostic imaging , Osteoarthritis, Hip/physiopathology , Prospective Studies , Prosthesis Design , Radiography , Range of Motion, Articular , Recovery of Function , Stress, Mechanical , Time Factors , Treatment Outcome , Weight-Bearing
7.
J Neurotrauma ; 28(3): 459-67, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21294701

ABSTRACT

Cervical myelopathy is caused by chronic segmental compression of the spinal cord because of degenerative changes of the spine. However, the exact mechanisms of chronic cervical cord compression are not fully understood. The purpose of this study was to validate a new animal model of chronic cervical cord compression capable of reproducing the clinical course without laminectomy in rats. A polyethylene line attached to a plastic plate was fastened with three turns around the vertebral body of C4 in 1-month-old rats. After surgery, the polyethylene line grows deeper into the dorsal wall of the spinal canal along with the growth of the spinal canal and vertebral body, producing a gradual compression of the spinal cord. The results show that this cervical canal stenosis (CCS) model in rats caused motor deficits and sensory disturbances 9 months after initiating CCS; however, no clinical manifestations took place until 6 months. The intramedullary high-intensity area on T2-weighted images was observed in 70% of the CCS model rats at 12 months after initiating CCS. In histological sections, the spinal cord was compressed along the entire circumference at 12 months after initiating CCS. The number of ventral neurons was decreased, and the white matter showed wallerian degeneration. This model might reproduce characteristic features of clinical chronic cervical cord compression, including progressive motor and sensory disturbances after a latency period and insidious neuronal loss, and represents chronic compression of the cervical spinal cord in humans.


Subject(s)
Models, Animal , Spinal Cord Compression/pathology , Spinal Cord Compression/physiopathology , Animals , Cervical Vertebrae , Laminectomy , Magnetic Resonance Imaging , Male , Motor Activity/physiology , Radiography , Rats , Rats, Sprague-Dawley , Spinal Cord Compression/diagnostic imaging
8.
Knee Surg Sports Traumatol Arthrosc ; 19(7): 1198-205, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21229231

ABSTRACT

PURPOSE: A lot of studies on the effect of intra-articular injections are clinical, but many questions on the effect of lidocaine to articular chondrocytes remain unanswered. This study was performed to determine the effects of varying concentrations and exposure times of lidocaine on the viability and proteoglycan metabolism of chondrocytes in vitro. METHOD: Cartilage was obtained from metatarsal joints of adult bovines. Chondrocytes in alginate beads were cultured in medium containing 6% fetal calf serum at 370 mOsmol at cell densities of 4 million cells/ml. They were then cultured for 24 h under 21% oxygen with 0.125, 0.25, 0.5, and 1% lidocaine and without lidocaine as control. The cell viability profile across intact beads was determined by manual counting using fluorescent probes and transmission electron microscopy. RESULT: Lactate production was measured enzymatically as a marker of energy metabolism. Glycosaminoglycan (GAG) accumulation was measured using a modified dimethylmethylene blue assay. Cell viability decreased in a time- and dose-dependent manner in the concentration range of 0.125-1.0% lidocaine under the confocal microscope. Under the electron microscope, apoptosis increased as the concentration of lidocaine increased. GAG accumulation/tissue volume decreases as the concentration of lidocaine increased. However, GAG produced per million cells and the rate of lactate production per live cell were significantly higher for cells cultured at 0.5 and 1% lidocaine than the control group. Bovine chondrocytes cultured in alginate beads under high oxygen pressure are negatively influenced by increasing concentrations of lidocaine. CONCLUSION: Cell viability and proteoglycan production (GAG accumulation/tissue volume) decreased as the concentration of lidocaine increased. These data suggest caution in prolonged exposure of cartilage to high concentration lidocaine. Repeated joint injection of lidocaine potentially worsens osteoarthrosis by accelerating cartilage degradation.


Subject(s)
Anesthetics, Local/toxicity , Cartilage, Articular/drug effects , Chondrocytes/drug effects , Glycosaminoglycans/metabolism , Lidocaine/toxicity , Proteoglycans/metabolism , Animals , Cartilage, Articular/cytology , Cattle , Cell Survival/drug effects , Chondrocytes/metabolism , Dose-Response Relationship, Drug , In Vitro Techniques , Lactic Acid/metabolism , Male , Metatarsus , Microscopy, Electron, Transmission , Time Factors
9.
Spine (Phila Pa 1976) ; 35(22): 2004-14, 2010 Oct 15.
Article in English | MEDLINE | ID: mdl-20959779

ABSTRACT

STUDY DESIGN: This study is aimed to investigate the changes of nerve root functions during the straight leg raising (SLR) test in vivo. OBJECTIVE: To investigate the relationship between nerve root movement and the electrophysiological values during an intraoperative SLR test. SUMMARY OF BACKGROUND DATA: The SLR test is one of the most significant signs for making a clinical diagnosis of lumbar disc herniation. A recent study showed that intraradicular blood flow apparently decreased during the SLR test in patients with disc herniation. METHODS: The study included 32 patients who underwent microdiscectomy. During the surgery, the nerve root motion affected by the hernia was observed during the SLR test. The patients' legs were allowed to hang down to the angle at which sciatica had occurred and the change of nerve root action potentials was measured. After removal of the hernia, a similar procedure was repeated. The periradicular specimens collected during surgery were examined by light and electron microscope. RESULTS: In all patients intraoperative microscopy revealed that the hernia was adherent to the dura mater of the nerve roots. During the SLR test, the limitation of nerve root movement occurred by periradicular adhesive tissue and amplitude of action potential showed a sharp decrease at the angle that produced sciatica. After removal of the hernia, all the patients showed smooth gliding of the nerve roots during the test, and there was no marked decrease of amplitude. Our data suggest that temporary ischemic changes in the nerve root cause transient conduction disturbances. Pathologic examination showed that the periradicular tissue consisted of the granulation with vascularization and many inflammatory cell infiltrations. CONCLUSION: The presence of periradicular fibrosis will compound the nerve root pain by fixing the nerve in one position, thereby increasing the susceptibility of the nerve root to tension or compression.


Subject(s)
Intervertebral Disc Displacement/physiopathology , Radiculopathy/physiopathology , Sciatica/physiopathology , Spinal Nerve Roots/physiopathology , Spondylosis/physiopathology , Tissue Adhesions/physiopathology , Adolescent , Adult , Female , Humans , Intervertebral Disc Displacement/pathology , Intervertebral Disc Displacement/surgery , Male , Middle Aged , Radiculopathy/etiology , Radiculopathy/pathology , Sciatica/etiology , Sciatica/pathology , Spinal Nerve Roots/pathology , Spondylosis/pathology , Spondylosis/surgery , Tissue Adhesions/etiology , Tissue Adhesions/pathology , Young Adult
10.
Joint Bone Spine ; 77(4): 351-4, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20537575

ABSTRACT

Hematoma of the lumbar ligamentum flavum is a very rare cause of sciatica. A 72-year-old man presented with left-sided sciatica and paresthesia of the lateral aspect of his left foot. From CT and MRI findings, he was diagnosed as having a hematoma embedded in the ligamentum flavum, which compressed the dura mater at the L5/S1 disc level. After an adequate surgical field was obtained with a microscope and a Casper retractor, the hematoma of the ligamentum flavum could be excised via a unilateral approach and satisfactory decompression of the cauda equina and nerve roots were obtained.


Subject(s)
Hematoma, Subdural, Spinal/diagnosis , Hematoma, Subdural, Spinal/surgery , Ligamentum Flavum/surgery , Lumbar Vertebrae/surgery , Aged , Hematoma, Subdural, Spinal/complications , Humans , Magnetic Resonance Imaging , Male , Microsurgery , Sciatica/etiology , Tomography, X-Ray Computed , Treatment Outcome
11.
J Neurosurg Spine ; 12(2): 197-202, 2010 Feb.
Article in English | MEDLINE | ID: mdl-20121356

ABSTRACT

OBJECT: So far, the morphological features of the vascular system supplying the dorsal root ganglion (DRG) have been inferred only from microangiograms. However, in the past most of these studies lacked 3D observations. This study presents the details of the microvasculature of the lumbar DRG visualized by scanning electron microscopy of vascular corrosion casts. METHODS: Wistar rats were anesthetized with intraperitoneal sodium pentobarbital. After thoracotomy, the vascular system was perfused with heparinized saline, and Mercox resin was injected into the thoracic aorta. After polymerization of the resin, the vascular casts were macerated with potassium hydroxide, washed with water, and dried. The casts were examined with a scanning electron microscope. RESULTS: The vascular cast of the DRG was observed to have a higher density of vessels than the nerve root. Bifurcation or anastomoses of capillaries took place at approximately right angles, in a T-shaped pattern. Within the DRG, both the arterial supply and the capillary network contained blood flow control structures (ring-shaped constrictions in the cast probably representing a vascular sphincter in the microvessel). Three types of vessels could be distinguished: tortuous, straight, and bead-like capillaries. The dilations, bulges, and tortuousness of capillaries could serve the function of locally increasing the capillary surface area in a sensory neuron. CONCLUSIONS: The results of this study suggest a causal relationship between the metabolic demands of local neuronal activity and both the density of the capillary network and the placement of the blood flow control structures.


Subject(s)
Ganglia, Spinal/blood supply , Ganglia, Spinal/ultrastructure , Lumbar Vertebrae , Microvessels/ultrastructure , Animals , Capillaries/ultrastructure , Corrosion Casting , Imaging, Three-Dimensional/methods , Microscopy, Electron, Scanning/methods , Rats , Rats, Wistar , Spinal Nerve Roots/blood supply , Spinal Nerve Roots/ultrastructure
12.
Joint Bone Spine ; 77(2): 184-6, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20138794

ABSTRACT

Discal cyst of the lumbar spine is a very rare cause of back pain and sciatica. We report two cases of discal cysts communicating with an adjacent herniated disc. From CT and MRI findings, they were diagnosed as having a discal cyst in the epidural space, which compressed the nerve root. After an adequate surgical field was obtained with a microscope and a Casper retractor, the discal cyst could be excised and satisfactory decompression of the adjacent nerve root was obtained. From histological and electron microscopic study, the presence of residual herniated tissues was confirmed in the cyst wall. Macrophages played an important role in the absorption of herniated tissue and the formation of the discal cyst. Hemorrhage in the cyst wall will make the serous hemorrhagic fluid-filled cystic structure in the absorbed spaces of the prolapsed disc. In this study, we confirmed that the discal cyst could have developed from the absorption process of a disc herniation.


Subject(s)
Epidermal Cyst/complications , Epidermal Cyst/pathology , Intervertebral Disc Displacement/etiology , Intervertebral Disc Displacement/pathology , Intervertebral Disc/pathology , Adult , Decompression, Surgical , Diagnosis, Differential , Epidermal Cyst/surgery , Humans , Intervertebral Disc/ultrastructure , Intervertebral Disc Displacement/surgery , Lumbar Vertebrae/pathology , Magnetic Resonance Imaging , Male , Microscopy, Electron, Transmission , Radiculopathy/etiology , Radiculopathy/pathology , Radiculopathy/surgery
13.
J Clin Neurosci ; 17(4): 501-5, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20122834

ABSTRACT

Entrapment neuropathy is a frequent clinical problem that can be caused by, among other factors, mechanical compression; however, exactly how a compressive force affects the peripheral nerves remains poorly understood. In this study, using a rabbit model of sciatic nerve injury (n=12), we evaluated the time-course of changes in intraneural blood flow, compound nerve action potentials, and functioning of the blood-nerve barrier during graded mechanical compression. Nerve injury was applied using a compressor equipped with a custom-made pressure transducer. Cessation of intraneural blood flow was noted at a mean compressive force of 0.457+/-0.022 N (+/-SEM), and the compound action potential became zero at 0.486+/-0.031 N. Marked extravasation of Evans blue albumin was noted after 20 min of intraneural ischemia. The functional changes induced by compression are likely due to intraneural edema, which could subsequently result in impairment of nerve function. These changes may be critical factors in the development of symptoms associated with nerve compression.


Subject(s)
Nerve Compression Syndromes/physiopathology , Sciatic Nerve/blood supply , Action Potentials/physiology , Animals , Blood-Nerve Barrier/physiopathology , Electrophysiology , Microscopy, Fluorescence , Rabbits , Regional Blood Flow/physiology , Sciatic Nerve/physiopathology , Stress, Mechanical
14.
J Orthop Res ; 28(8): 1022-5, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20108328

ABSTRACT

Phalen's test has been one of the most significant of clinical signs when making a clinical diagnosis of idiopathic carpal tunnel syndrome (CTS). However, it is unknown whether intraneural blood flow changes during Phalen's test in patients with CTS. In this study, an intraoperative Phalen's test was conducted in patients with CTS to observe the changes in intraneural blood flow using a laser Doppler flow meter. During Phalen's test, intraneural blood flow showed a sharp decrease, which lasted for 1 min. Intraneural blood flow decreased by 56.7%-100% (average, 78.0%) in the median nerve relative to the blood flow before the test. At 1 min after completing the test, intraneural blood flow returned to the baseline value. After carpal tunnel release, there was no marked decrease in intraneural blood flow. This study demonstrated that the blood flow in the median nerve is reduced when Phalen's test is performed in vivo.


Subject(s)
Carpal Tunnel Syndrome/physiopathology , Median Nerve/blood supply , Neural Conduction , Adult , Aged , Electrodiagnosis , Female , Humans , Male , Median Nerve/physiopathology , Middle Aged
15.
Joint Bone Spine ; 76(6): 705-7, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19945898

ABSTRACT

A rare case of parosteal aneurysmal bone cyst arising from the humerus in a 7-year-old boy is reported. Plain radiography and CT scanning revealed a marked periosteal reaction and a soft tissue mass surrounded by bone. Erosion of the cortex did not extend to involve the medulla. Three-dimensional CT scanning (3-DCT) revealed a birdcage-like ossified structure that was attached to the cortex of the humerus. 3-DCT was also used to visualize the extent of the tumor and the architecture of the bone, providing important information for planning of surgery. Inside the tumor, there were a number of fluid-fluid levels on T1-weighted MRI and high intensity areas on T2-weighted images. Pathologic examination revealed a central cavity containing a large volume of blood in the tumor and reactive osteogenesis mainly at the marginal regions, and these findings reflected the birdcage-like ossified structure observed by 3-DCT. Pathological examination was required to rule out osteosarcoma in particular, but a diagnosis of parosteal aneurysmal bone cyst was made because of the absence of findings suggestive of malignancy. The tumor was excised en bloc with a clear margin. At 3 years after surgery, there is no evidence of recurrent tumor.


Subject(s)
Bone Cysts, Aneurysmal/pathology , Ossification, Heterotopic/pathology , Bone Cysts, Aneurysmal/diagnostic imaging , Bone Cysts, Aneurysmal/surgery , Bone Neoplasms/diagnosis , Child , Diagnosis, Differential , Humans , Humerus/diagnostic imaging , Humerus/pathology , Male , Osteosarcoma/diagnosis , Radiography , Treatment Outcome
16.
Spine (Phila Pa 1976) ; 34(26): E945-51, 2009 Dec 15.
Article in English | MEDLINE | ID: mdl-20010383

ABSTRACT

STUDY DESIGN.: To examine whether lidocaine cytotoxicity to chondrocytes has been implicated in the development of osteoarthritis of the zygapophysial joints. OBJECTIVE.: This study was performed to determine the effects of varying concentrations and exposure times of lidocaine on the viability and proteoglycan metabolism of rabbit zygapophysial chondrocytes in vitro. SUMMARY OF BACKGROUND DATA.: Zygapophysial joint injections are commonly administered with lidocaine for chronic spinal pain in orthopedic treatment. A lot of studies on the effect of zygapophysial joint injections are clinical, but many questions on the effect of lidocaine to zygapophysial chondrocytes remain unanswered. METHODS.: Cartilage was obtained from zygapophysial joints of adult rabbits. Chondrocytes in alginate beads were cultured in medium containing 6% fetal calf serum at 370 mOsmol at cell densities of 4 million cells/mL. They were then cultured for 24 hours under 21% oxygen with 0.125%, 0.25%, 0.5%, and 1% lidocaine, and without lidocaine as control. The cell viability profile across intact beads was determined by manual counting using fluorescent probes (LIVE/DEAD assay) and transmission electron microscopy. Lactate production was measured enzymatically as a marker of energy metabolism. Glycosaminoglycan (GAG) accumulation was measured using a modified dimethylmethylene blue assay. RESULTS.: Cell viability decreased in a time- and dose-dependent manner in the concentration range of 0.125% to 1.0% lidocaine under the confocal microscope. Under the electron microscope, apoptosis increased as the concentration of lidocaine increased. GAG accumulation/tissue volume decreases as the concentration of lidocaine increased. However, GAG produced per million cells and the rate of lactate production per live cell was significantly higher for cells cultured at 0.5% and 1% lidocaine than the control group. CONCLUSION.: While these in vitro results cannot be directly extrapolated to the clinical setting, this data suggestcaution in prolonged exposure of zygapophysial cartilage to high concentration lidocaine.


Subject(s)
Chondrocytes/drug effects , Lidocaine/toxicity , Proteoglycans/metabolism , Zygapophyseal Joint/drug effects , Analysis of Variance , Animals , Cartilage, Articular/drug effects , Cartilage, Articular/metabolism , Cell Count , Cell Survival/drug effects , Cells, Cultured , Chondrocytes/metabolism , Chondrocytes/pathology , Dose-Response Relationship, Drug , Glycosaminoglycans/metabolism , Lactic Acid/biosynthesis , Microscopy, Confocal , Microscopy, Electron, Transmission , Osteoarthritis/chemically induced , Osteoarthritis/metabolism , Osteoarthritis/pathology , Rabbits , Time Factors , Zygapophyseal Joint/metabolism , Zygapophyseal Joint/pathology
17.
J Orthop Sci ; 14(5): 471-83, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19802657

ABSTRACT

BACKGROUND: Molecular biological techniques such as the polymerase chain reaction (PCR) and DNA microarray are used for the detection/identification of microorganisms; however, few reports have discussed the clinical utility of microarray analysis for identification of causative organisms of osteoarticular infections. It is important to examine the utility of PCR amplification followed by analysis of DNA microarray carrying specific oligonucleotides. METHODS: This study included 101 biological samples obtained from 96 patients who underwent conservative and/or surgical treatment for osteoarticular infections. In this double-blind comparative study, routine conventional testing and the research groups were unaware of each other's interpretation until identical specimens were identified by culture and microarray analysis. RESULTS: Results of PCR microarray analysis were positive for 25 samples and negative for the remaining 76 samples within 24 h, and the results of the cultures (available after a mean of 3.54 days) were positive in 26 samples and negative for the remaining 75 samples. The sensitivity of microarray analysis was 84.6% (22/26) and specificity was 88.0% (22/25). Discrepant results were identified in seven samples, including a negative culture and a positive microarray in three cases and a positive culture and a negative microarray in four other cases. CONCLUSIONS: The PCR microarray analysis is complementary to routine cultures in identifying causative microorganisms and should be used in patients with highly suspected infections and negative bacterial culture and in patients who require prompt diagnosis and early initiation of antibiotic therapy.


Subject(s)
Bone Diseases, Infectious/microbiology , DNA Probes , Oligonucleotide Array Sequence Analysis , Polymerase Chain Reaction/methods , Double-Blind Method , Humans , Sensitivity and Specificity
18.
Tissue Eng Part A ; 15(12): 3835-46, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19681728

ABSTRACT

The nucleus pulposus (NP) of the intervertebral disc in cattle and humans shows the most dramatic changes with aging of any cartilaginous tissue. In humans, notochordal cells disappear from the NP and are replaced with chondrocytic cells by adolescence. However, notochordal cells of the NP persist into adult life in some species, such as rats and rabbits. Therefore, comparison of the metabolic activity of notochordal and nonnotochordal cells is considered to be important for determining the type of cell to use for transplantation to regenerate intervertebral discs. In this study, we investigated the notochordal NP cells of rats and rabbits, as well as nonnotochordal (chondrocyte-like) bovine NP cells, in a three-dimensional culture system to examine whether proteoglycan metabolism varied among these three cell types. As a result, bovine NP cells produced around 0.18 mg/mL of glycosaminoglycan after culture for 5 days, while rat and rabbit NP cells produced about four and two times more glycosaminoglycan than bovine cells, respectively. In conclusion, this study demonstrated marked differences of energy metabolism and production of matrix components between notochordal and nonnotochordal NP cells. Animals with notochordal cells in the NP, such as rats and rabbits, may not provide good models for investigation of biological repair and tissue engineering for human disc disorders.


Subject(s)
Cell Separation/methods , Intervertebral Disc/pathology , Proteoglycans/biosynthesis , Spinal Diseases/pathology , Tail/cytology , Tissue Engineering/methods , Wound Healing , Animals , Cattle , Cell Shape , Cells, Cultured , Disease Models, Animal , Extracellular Space/metabolism , Glycosaminoglycans/biosynthesis , Humans , Intervertebral Disc/metabolism , Intervertebral Disc/transplantation , Intervertebral Disc/ultrastructure , Male , Models, Biological , Phenotype , Rabbits , Rats
19.
Spine (Phila Pa 1976) ; 34(10): 990-7, 2009 May 01.
Article in English | MEDLINE | ID: mdl-19404173

ABSTRACT

STUDY DESIGN: This study is to investigate the changes of vasomotion of intraradicular microvessels in vivo. OBJECTIVE: We have observed microvascular corrosion casts of the lumbar nerve root by scanning electron microscopy and used an immunohistochemical technique to investigate the presence and distribution of autonomic and sensory nerve in blood vessels of the nerve root. SUMMARY OF BACKGROUND DATA: It is generally considered that the genesis of radiculopathy associated with the degenerative conditions of the spine may result from both mechanical compression and circulatory disturbance. However, the neurogenic control of intraradicular blood flow has received little attention in the past. METHODS: For three-dimensional observation of intraradicular vessels, we used scanning electron microscopic examination of microvascular corrosion casts in ten Wister rats. To investigate the mechanism of vasomotion of the nerve root, we used immunohistochemical methods. The sections were incubated overnight with antisera to tyrosine hydroxylase, choline acetyl transferase, substance P, calcitonin-gene-related peptide, vasoactive intestinal peptide, somatostatin, neuropeptide Y, leucine-enkephalin, cholecystokinin octapeptide, brain-nitric oxide synthase, and endothelium-nitric oxide synthase. Abidin-biotin complex method was used as the immunohistochemical procedure and the sections were observed under the light microscope. RESULTS: The general view of whole vascular casts of the lumbar spinal cord and nerve roots showed a high density of vessels. Bifurcation or anastomoses of capillaries approximately took place at right angles in a T-shaped pattern and capillaries showed a lot of ring-like compressions. This ring-like compression on the cast may represent a vascular sphincter in the microvessels. This study also reveals the existence of perivascular adrenergic, cholinergic, peptidergic, and nitroxydergic innervation with a possible role in neurogenic regulation of nerve root circulation. CONCLUSION: Perivascular nerve plexuses around intraradicular microvessels suggest that the autonomic nerves play an important role in intraradicular circulation.


Subject(s)
Autonomic Pathways/ultrastructure , Blood Vessels/innervation , Blood Vessels/ultrastructure , Microcirculation/physiology , Spinal Nerve Roots/blood supply , Vasomotor System/ultrastructure , Animals , Autonomic Pathways/metabolism , Blood Vessels/metabolism , Capillaries/physiology , Capillaries/ultrastructure , Corrosion Casting/methods , Immunohistochemistry , Male , Microscopy, Electron, Scanning , Muscle, Smooth, Vascular/innervation , Muscle, Smooth, Vascular/metabolism , Muscle, Smooth, Vascular/ultrastructure , Neuropeptides/metabolism , Neurotransmitter Agents/metabolism , Nitric Oxide/metabolism , Radiculopathy/metabolism , Radiculopathy/physiopathology , Rats , Rats, Wistar , Regional Blood Flow/physiology , Sensory Receptor Cells/metabolism , Sensory Receptor Cells/ultrastructure , Spinal Nerve Roots/physiology , Vasoconstriction/physiology , Vasomotor System/metabolism
20.
J Neurotrauma ; 26(7): 1167-75, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19331518

ABSTRACT

It is generally believed that radiculopathy associated with the degenerative conditions of the spine may result from both mechanical compression and circulatory disturbance. However, the basic pathophysiology of circulatory disturbance induced by ischemia and congestion is not fully understood. This study investigated the effect of ischemia and congestion on the dorsal root ganglion (DRG) using an in vivo model. The sixth and seventh lumbar laminae were removed and the seventh lumbar DRG was exposed using adult dogs. The aorta was clamped as an ischemic model in the DRG, and the inferior vena cava was clamped as a congestion model at the sixth costal level for 30 min using forceps transpleurally. Measurements of blood flow, partial oxygen pressure, and action potentials in the DRG were recorded over a period of 1 h after clamp release. Finally, we examined the status of intraganglionic blood permeability under a fluorescence microscope following injection of Evans blue albumin into the cephalic vein to determine the type of circulatory disturbance occurring in the DRG. Immediately after inferior vena cava clamping, the central venous pressure increased approximately four times and marked extravasation of protein tracers was induced in the lumbar DRG. Blood flow, partial oxygen pressures, and action potentials within the DRG were more severely affected by the aorta clamping; however, this ischemic model did not reveal any permeability changes in the DRG. The permeability change in the DRG was more easily increased via venous congestion than by arterial ischemia. The intraganglionic venous flow was stopped with compression at much lower pressures than that needed to impact arterial flow. From a clinical perspective, intraganglionic edema formation, rather than arterial ischemia, may be an earlier phenomenon inducing DRG dysfunction.


Subject(s)
Action Potentials/physiology , Capillary Permeability/physiology , Ganglia, Spinal/blood supply , Hyperemia/physiopathology , Ischemia/physiopathology , Analysis of Variance , Animals , Aorta/physiopathology , Dogs , Electrophysiology , Ganglia, Spinal/physiopathology , Radiculopathy/physiopathology , Regional Blood Flow/physiology
SELECTION OF CITATIONS
SEARCH DETAIL
...