ABSTRACT
A metal-based fluorescent probe for H2O2, named MBFh2, releases a highly fluorescent resorufin in the seconds time scale even in the presence of 5 µM H2O2. The use of MBFh2 enabled the visualization of intracellular H2O2 that was generated after stimulation of the epidermal growth factor.
Subject(s)
Coordination Complexes/chemistry , Fluorescent Dyes/chemistry , Hydrogen Peroxide/analysis , Iron/chemistry , HeLa Cells/chemistry , Humans , Molecular StructureABSTRACT
A mononuclear iron(III) complex of a noncyclic tetradentate monoamido ligand, Fe(III)mpaq, catalyses the oxidation of Orange II, guaiacol, ABTS and Amplex Red with H2O2 in aqueous solutions at neutral pH. Under identical conditions, other structurally related nonheme iron complexes showed only negligible activities.
Subject(s)
Biomimetic Materials/metabolism , Coordination Complexes/metabolism , Ferric Compounds/metabolism , Azo Compounds/metabolism , Benzenesulfonates/metabolism , Benzothiazoles/metabolism , Biomimetic Materials/chemical synthesis , Catalysis , Coordination Complexes/chemical synthesis , Crystallography, X-Ray , Ferric Compounds/chemical synthesis , Guaiacol/metabolism , Models, Molecular , Oxazines/metabolism , Oxidation-Reduction , Sulfonic Acids/metabolismABSTRACT
We developed a metal-based fluorescent probe for H(2)O(2) called MBFh1, which has an iron complex as a reaction site for H(2)O(2) and a 3,7-dihydroxyphenoxazine derivative as the fluorescent reporter unit. The iron complex reacts quickly with H(2)O(2) to form oxidants, and then the oxidants convert the closely appended nonfluorescent 3,7-dihydroxyphenoxazine moiety to resorufin in an intramolecular fashion. The quick response to H(2)O(2) allows us to plot the enzymatic evolution of H(2)O(2). A combination of N-acetyl-3,7-dihydroxyphenoxazine and horseradish peroxidase has been frequently used to detect enzymatically generated H(2)O(2), but this method has interference with phenol derivatives. The use of MBFh1 overcomes this drawback.
