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1.
J Chem Phys ; 155(7): 075101, 2021 Aug 21.
Article in English | MEDLINE | ID: mdl-34418942

ABSTRACT

Intercellular fluids in living organisms contain high concentrations of macromolecules such as nucleic acid and protein. Over the past few decades, several studies have examined membraneless organelles in terms of liquid-liquid phase separation. These studies have investigated aggregation/attraction among a rich variety of biomolecules. Here, we studied the association between the polymerization/depolymerization of actin, interconversion between monomeric (G-actin) and filamentous states (F-actin), and water/water phase separation in a binary polymer solution using polyethylene glycol (PEG) and dextran (DEX). We found that actin, which is a representative cytoskeleton, changes its distribution in a PEG/DEX binary solution depending on its polymerization state: monomeric G-actin is distributed homogeneously throughout the solution, whereas polymerized F-actin is localized only within the DEX-rich phase. We extended our study by using fragmin, which is a representative actin-severing and -depolymerizing factor. It took hours to restore a homogeneous actin distribution from localization within the DEX-rich phase, even with the addition of fragmin in an amount that causes complete depolymerization. In contrast, when actin that had been depolymerized by fragmin in advance was added to a solution with microphase-separation, F-actin was found in DEX-rich phase droplets. The micro-droplets tended to deform into a non-spherical morphology under conditions where they contained F-actin. These findings suggest that microphase-separation is associated with the dynamics of polymerization and localization of the actin cytoskeleton. We discuss our observations by taking into consideration the polymer depletion effect.


Subject(s)
Actins/chemistry , Protein Multimerization , Dextrans/chemistry , Models, Molecular , Polyethylene Glycols/chemistry , Protein Structure, Quaternary , Solutions , Water/chemistry
2.
Membranes (Basel) ; 11(5)2021 May 03.
Article in English | MEDLINE | ID: mdl-34063660

ABSTRACT

We characterized the size, distribution, and fluidity of microdomains in a lipid bilayer containing phosphatidylinositol (PI) and revealed their roles during the two-dimensional assembly of a membrane deformation protein (FBP17). The morphology of the supported lipid bilayer (SLB) consisting of PI and phosphatidylcholine (PC) on a mica substrate was observed with atomic force microscope (AFM). Single particle tracking (SPT) was performed for the PI+PC-SLB on the mica substrate by using the diagonal illumination setup. The AFM topography showed that PI-derived submicron domains existed in the PI+PC-SLB. The spatiotemporal dependence of the lateral lipid diffusion obtained by SPT showed that the microdomain had lower fluidity than the surrounding region and worked as the obstacles for the lipid diffusion. We observed the two-dimensional assembly of FBP17, which is one of F-BAR family proteins included in endocytosis processes and has the function generating lipid bilayer tubules in vitro. At the initial stage of the FBP17 assembly, the PI-derived microdomain worked as a scaffold for the FBP17 adsorption, and the fluid surrounding region supplied FBP17 to grow the FBP17 domain via the lateral molecular diffusion. This study demonstrated an example clearly revealing the roles of two lipid microregions during the protein reaction on a lipid bilayer.

3.
Chembiochem ; 21(23): 3323-3328, 2020 12 01.
Article in English | MEDLINE | ID: mdl-32667694

ABSTRACT

Recently, liquid-liquid phase separation (LLPS) has attracted considerable attention among researchers in the life sciences as a plausible mechanism for the generation of microstructures inside cells. LLPS occurs through multiple nonspecific interactions and does not always require a lock-and-key interaction with a binary macromolecular solution. The remarkable features of LLPS include the non-uniform localization and concentration of solutes, resulting in the ability to isolate certain chemical systems and thereby parallelize multiple chemical reactions within the limited space of a living cell. We report that, by using the macromolecules, poly(ethylene glycol) (PEG) and dextran, that exhibit LLPS in an aqueous solution, cell-sized liposomes are spontaneously formed therein in the presence of phospholipids. In this system, LLPS is generated through the depletion effect of macromolecules. The results showed that cell-like microdroplets entrapping DNA wrapped by a phospholipid layer emerge in a self-organized manner.


Subject(s)
Dextrans/chemistry , Lipid Droplets/chemistry , Polyethylene Glycols/chemistry , DNA/chemistry , Macromolecular Substances/chemistry , Particle Size , Phospholipids/chemistry , Solutions , Water/chemistry
4.
Biomolecules ; 10(5)2020 05 08.
Article in English | MEDLINE | ID: mdl-32397190

ABSTRACT

Depolymerization and polymerization of the actin filament are indispensable in eukaryotes. The DNase I binding loop (D-loop), which forms part of the interface between the subunits in the actin filament, is an intrinsically disordered loop with a large degree of conformational freedom. Introduction of the double mutation G42A/G46A to the D-loop of the beta cytoskeletal mammalian actin restricted D-loop conformational freedom, whereas changes to the critical concentration were not large, and no major structural changes were observed. Polymerization and depolymerization rates at both ends of the filament were reduced, and cofilin binding was inhibited by the double mutation. These results indicate that the two glycines at the tip of the D-loop are important for actin dynamics, most likely by contributing to the large degree of conformational freedom.


Subject(s)
Actins/genetics , Actins/metabolism , Mutation/genetics , Actin Cytoskeleton/metabolism , Actin Cytoskeleton/ultrastructure , Actin Depolymerizing Factors/metabolism , Actin Depolymerizing Factors/ultrastructure , Actins/ultrastructure , Amino Acid Sequence , Humans , Models, Molecular , Polymerization , Protein Binding , Protein Structure, Secondary , Protein Subunits/metabolism , Recombinant Proteins/isolation & purification
5.
Biophys Rev ; 12(2): 425-434, 2020 Apr.
Article in English | MEDLINE | ID: mdl-32144739

ABSTRACT

Recently, the important role of microphase separation in living cells has been attracting considerable interest in relation to cell organization and function. For example, many studies have focused on liquid-liquid phase separation (LLPS) as a very plausible mechanism for the presence of membraneless organelles. To confirm the role of phase separation in living cells, experimental studies on models and/or reconstructed systems are needed. In this short review, we discuss current paradigms of LLPS and provide some example "review data" to demonstrate particular points relating to the specific localization of biological macromolecules like DNAs and actin proteins with spontaneous domain formation in microdroplets emerging in an aqueous two-phase system (ATPS) (we use polyethylene glycol (PEG)/dextran (DEX)-a binary polymer solution). We also suggest that phase separation and transition may play basic roles in regulation of the biochemical reactivity of individual long genomic DNAs.

6.
Chembiochem ; 19(13): 1370-1374, 2018 07 04.
Article in English | MEDLINE | ID: mdl-29676062

ABSTRACT

The effect of binary hydrophilic polymers on a pair of representative bio-macromolecules in a living cell has been examined. The results showed that these bio-macromolecules exhibited specific localization in cell-sized droplets that were spontaneously formed through water/water microphase segregation under crowding conditions with coexisting polymers. In these experiments, a simple binary polymer system with poly(ethylene glycol) (PEG) and dextran (DEX) was used. Under the conditions of microphase segregation, DNA was entrapped within cell-sized droplets rich in DEX. Similarly, F-actin, linearly polymerized actin, was entrapped specifically within microdroplets rich in DEX, whereas G-actin, a monomeric actin, was distributed evenly inside and outside these droplets. This study has been extended to a system with both F-actin and DNA, and it was found that DNA molecules were localized separately from aligned F-actin proteins to create microdomains inside microdroplets, reflecting the self-emergence of a cellular morphology similar to a stage of cell division.


Subject(s)
Actins/chemistry , Artificial Cells/chemistry , DNA/chemistry , Water/chemistry , Animals , Chickens , Dextrans/chemistry , Polyethylene Glycols/chemistry
7.
Biol Pharm Bull ; 41(3): 288-293, 2018.
Article in English | MEDLINE | ID: mdl-29491204

ABSTRACT

For the development of artificial cell-like machinery, liposomes encapsulating cytoskeletons have drawn much recent attention. However, there has been no report showing isothermally reversible morphological changes of liposomes containing cytoskeletons. We succeeded in reversibly changing the shape of cell-sized giant vesicles by controlling the polymerization/depolymerization state of cytoskeletal microtubules that were encapsulated in the vesicles using pressure changes. The result indicates that it is possible to manipulate artificial cell models composed of molecules such as lipids and proteins. The findings obtained in this study will be helpful in clarifying the details of cooperation between cytoskeletal dynamics and morphogenesis of biological membranes and in improving the design and construction of further advanced artificial cell-like machinery, such as drug-delivery systems. In addition, the experimental system used in this study can be applied to research to elucidate the adaptive strategy of living organisms to external stimuli and extreme conditions such as osmotic stress and high-pressure environments like the deep sea.


Subject(s)
Artificial Cells , Coated Vesicles , Microtubules/chemistry , Animals , Cytoskeleton/chemistry , Cytoskeleton/ultrastructure , Drug Carriers , Drug Delivery Systems , Hydrostatic Pressure , Lipid Bilayers , Liposomes/chemistry , Osmotic Pressure , Particle Size , Swine , Tubulin/chemistry
8.
Colloids Surf B Biointerfaces ; 155: 248-256, 2017 Jul 01.
Article in English | MEDLINE | ID: mdl-28432958

ABSTRACT

Giant proteoliposomes are generally useful as artificial cell membranes in biochemical and biophysical studies, and various procedures for their preparation have been reported. We present here a novel preparation technique that involves the combination of i) cell-sized lipid vesicles (giant unilamellar vesicles, GUVs) that are generated using the droplet-transfer method, where lipid monolayer-coated water-in-oil microemulsion droplets interact with oil/water interfaces to form enclosed bilayer vesicles, and ii) budded viruses (BVs) of baculovirus (Autographa californica nucleopolyhedrovirus) that express recombinant transmembrane proteins on their envelopes. GP64, a fusogenic glycoprotein on viral envelopes, is activated by weak acids and is thought to cause membrane fusion with liposomes. Using confocal laser scanning microscopy (CLSM), we observed that the single giant liposomes fused with octadecyl rhodamine B chloride (R18)-labeled wild-type BV envelopes with moderate leakage of entrapped soluble compounds (calcein), and the fusion profile depended on the pH of the exterior solution: membrane fusion occurred at pH ∼4-5. We further demonstrated that recombinant transmembrane proteins, a red fluorescent protein (RFP)-tagged GPCR (corticotropin-releasing hormone receptor 1, CRHR1) and envelope protein GP64 could be partly incorporated into membranes of the individual giant liposomes with a reduction of the pH value, though there were also some immobile fluorescent spots observed on their circumferences. This combination may be useful for preparing giant proteoliposomes containing the desired membranes and inner phases.


Subject(s)
Baculoviridae/chemistry , Proteolipids/chemistry , Receptors, Corticotropin-Releasing Hormone/chemistry , Unilamellar Liposomes/chemistry , Viral Envelope Proteins/chemistry , Fluoresceins/chemistry , Fluorescent Dyes/chemistry , Humans , Hydrogen-Ion Concentration , Luminescent Proteins/chemistry , Membrane Fusion , Recombinant Proteins/chemistry , Rhodamines/chemistry , Solutions , Spectrometry, Fluorescence , Red Fluorescent Protein
9.
Chem Commun (Camb) ; 53(24): 3458-3461, 2017 Mar 25.
Article in English | MEDLINE | ID: mdl-28271115

ABSTRACT

The first in vitro selection of binding peptides against artificial lipid membranes from a random peptide library using an in vitro display method (cDNA display) is reported. The selected peptide, LB-1, has both amphiphilic and cationic regions, and proteins fused to LB-1 can be immobilized on the liposome surface.


Subject(s)
Lipid Bilayers/metabolism , Peptides/metabolism , Amino Acid Sequence , DNA, Complementary/chemistry , DNA, Complementary/metabolism , Lipid Bilayers/chemistry , Liposomes/chemistry , Liposomes/metabolism , Microscopy, Confocal , Peptides/chemistry , Polymerase Chain Reaction , Surface Plasmon Resonance
10.
Langmuir ; 32(48): 12823-12832, 2016 12 06.
Article in English | MEDLINE | ID: mdl-27934514

ABSTRACT

Domain formation or compartmentalization in a lipid bilayer membrane has been thought to take place dynamically in cell membranes and play important roles in the spatiotemporal regulation of their physiological functions. In addition, the membrane skeleton, which is a protein assembly beneath the cell membrane, also regulates the properties as well as the morphology of membranes because of its role as a diffusion barrier against constitutive molecules of the membrane or as a scaffold for physiological reactions. Therefore, it is important to study the relationship between lipid bilayer membranes and proteins that form the membrane skeleton. Among cytoskeletal systems, septin is unique because it forms arrays on liposomes that contain phosphoinositides, and this property is thought to contribute to the formation of the annulus in sperm flagellum. In this study, a supported lipid bilayer (SLB) was used to investigate the effect of septin on lipid bilayers because SLBs rather than liposomes are suitable for observation of the membrane domains formed. We found that SLBs containing phosphatidylinositol (PI) reversibly form domains by decreasing the temperature and that septin affects both the formation and the disappearance of the cooling-induced domain. Septin inhibits the growth of cooling-induced domains during decreases in temperature and inhibits the dispersion and the disappearance of those domains during increases in temperature. These results indicate that septin complexes, i.e., filaments or oligomers assembling on the surface of lipid bilayer membranes, can regulate the dynamics of domain formation via their behavior as an anchor for PI molecules.


Subject(s)
Cell Membrane , Lipid Bilayers , Septins/pharmacology , Phase Transition , Temperature
11.
Langmuir ; 32(15): 3794-802, 2016 Apr 19.
Article in English | MEDLINE | ID: mdl-27023063

ABSTRACT

Liposomes encapsulating cytoskeletons have drawn much recent attention to develop an artificial cell-like chemical-machinery; however, as far as we know, there has been no report showing isothermally reversible morphological changes of liposomes containing cytoskeletons because the sets of various regulatory factors, that is, their interacting proteins, are required to control the state of every reaction system of cytoskeletons. Here we focused on hydrostatic pressure to control the polymerization state of microtubules (MTs) within cell-sized giant liposomes (diameters ∼10 µm). MT is the cytoskeleton formed by the polymerization of tubulin, and cytoskeletal systems consisting of MTs are very dynamic and play many important roles in living cells, such as the morphogenesis of nerve cells and formation of the spindle apparatus during mitosis. Using real-time imaging with a high-pressure microscope, we examined the effects of hydrostatic pressure on the morphology of tubulin-encapsulating giant liposomes. At ambient pressure (0.1 MPa), many liposomes formed protrusions due to tubulin polymerization within them. When high pressure (60 MPa) was applied, the protrusions shrank within several tens of seconds. This process was repeatedly inducible (around three times), and after the pressure was released, the protrusions regenerated within several minutes. These deformation rates of the liposomes are close to the velocities of migrating or shape-changing living cells rather than the shortening and elongation rates of the single MTs, which have been previously measured. These results demonstrate that the elongation and shortening of protrusions of giant liposomes is repeatedly controllable by regulating the polymerization state of MTs within them by applying and releasing hydrostatic pressure.


Subject(s)
Liposomes/chemistry , Tubulin/chemistry , Animals , Hydrostatic Pressure , Protein Structure, Quaternary , Swine
12.
Chemphyschem ; 17(4): 471-3, 2016 Feb 16.
Article in English | MEDLINE | ID: mdl-26891092

ABSTRACT

We observed single DNA molecules at different ethanol concentrations by using fluorescence microscopy. Large single DNA molecules undergo reentrant conformational transitions from elongated coil into folded globule and then into elongated coil state, accompanied by the increase of the concentration of ethanol in a low-salt aqueous environment. The second transition from globule into the coil state occurs at around 70 % (v/v) ethanol. From circular dichroism (CD) measurements, it is confirmed that the reentrant transition of the higher order structure proceeds together with the transitions of the secondary structure from B to C and, then, from C to A in a cooperative manner. The determined mechanism of the reentrant transition is discussed in relation to the unique characteristics of solutions with higher ethanol content, for which clathrate-like nanostructures of alcohol molecules are generated in the surrounding water.


Subject(s)
DNA/chemistry , Ethanol/chemistry , Solvents/chemistry , Circular Dichroism , Microscopy, Fluorescence , Nucleic Acid Conformation
13.
Membranes (Basel) ; 5(1): 22-47, 2015 Jan 20.
Article in English | MEDLINE | ID: mdl-25611306

ABSTRACT

The mechanical properties of cell-sized giant unilamellar liposomes were studied by manipulating polystyrene beads encapsulated within the liposomes using double-beam laser tweezers. Mechanical forces were applied to the liposomes from within by moving the beads away from each other, which caused the liposomes to elongate. Subsequently, a tubular membrane projection was generated in the tip at either end of the liposome, or the bead moved out from the laser trap. The force required for liposome transformation reached maximum strength just before formation of the projection or the moving out of the bead. By employing this manipulation system, we investigated the effects of membrane lipid compositions and environment solutions on the mechanical properties. With increasing content of acidic phospholipids, such as phosphatidylglycerol or phosphatidic acid, a larger strength of force was required for the liposome transformation. Liposomes prepared with a synthetic dimyristoylphosphatidylcholine, which has uniform hydrocarbon chains, were transformed easily compared with liposomes prepared using natural phosphatidylcholine. Surprisingly, bovine serum albumin or fetuin (soluble proteins that do not bind to membranes) decreased liposomal membrane rigidity, whereas the same concentration of sucrose showed no particular effect. These results show that the mechanical properties of liposomes depend on their lipid composition and environment.

14.
Orig Life Evol Biosph ; 44(4): 325-9, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25585806

ABSTRACT

Eukaryotes, by the same combination of cytoskeleton and molecular motor, for example actin filament and myosin, can generate a variety of movements. For this diversity, the organization of biological machineries caused by the confinement and/or crowding effects of internal living cells, may play very important roles.


Subject(s)
Actin Cytoskeleton/chemistry , Actins/chemistry , Myosins/chemistry , Origin of Life , Artificial Cells/chemistry , Artificial Cells/ultrastructure , Cell Size , Liposomes/chemistry , Liposomes/ultrastructure , Models, Biological
15.
Toxins (Basel) ; 5(4): 637-64, 2013 Apr 17.
Article in English | MEDLINE | ID: mdl-23594437

ABSTRACT

Melittin induces various reactions in membranes and has been widely studied as a model for membrane-interacting peptide; however, the mechanism whereby melittin elicits its effects remains unclear. Here, we observed melittin-induced changes in individual giant liposomes using direct real-time imaging by dark-field optical microscopy, and the mechanisms involved were correlated with results obtained using circular dichroism, cosedimentation, fluorescence quenching of tryptophan residues, and electron microscopy. Depending on the concentration of negatively charged phospholipids in the membrane and the molecular ratio between lipid and melittin, melittin induced the "increasing membrane area", "phased shrinkage", or "solubilization" of liposomes. In phased shrinkage, liposomes formed small particles on their surface and rapidly decreased in size. Under conditions in which the increasing membrane area, phased shrinkage, or solubilization were mainly observed, the secondary structure of melittin was primarily estimated as an α-helix, ß-like, or disordered structure, respectively. When the increasing membrane area or phased shrinkage occurred, almost all melittin was bound to the membranes and reached more hydrophobic regions of the membranes than when solubilization occurred. These results indicate that the various effects of melittin result from its ability to adopt various structures and membrane-binding states depending on the conditions.


Subject(s)
Insect Proteins/chemistry , Lipid Bilayers/chemistry , Melitten/chemistry , Membrane Proteins/chemistry , Phospholipids/chemistry , Animals , Chemical Phenomena , Circular Dichroism , Hydrophobic and Hydrophilic Interactions , Insect Proteins/metabolism , Kinetics , Lipid Bilayers/metabolism , Liposomes , Melitten/metabolism , Membrane Proteins/metabolism , Membranes/chemistry , Membranes/metabolism , Membranes/ultrastructure , Microscopy, Electron, Transmission , Phosphatidylcholines/chemistry , Phosphatidylcholines/metabolism , Phosphatidylglycerols/chemistry , Phosphatidylglycerols/metabolism , Phospholipids/metabolism , Protein Structure, Secondary , Solubility , Surface Properties , Tryptophan/chemistry
16.
Langmuir ; 29(1): 328-36, 2013 Jan 08.
Article in English | MEDLINE | ID: mdl-23199228

ABSTRACT

The Fer-CIP4 homology-BAR (F-BAR) domain, which was identified as a biological membrane-deforming module, has been reported to transform lipid bilayer membranes into tubules. However, details of the tubulation process, the mechanism, and the properties of the generated tubules remain unknown. Here, we successfully monitored the entire process of tubulation and the behavior of elongated tubules caused by four different F-BAR domain family proteins (FBP17, CIP4, PSTPIP1, and Pacsin2) using direct real-time imaging of giant unilamellar liposomes with dark-field optical microscopy. FBP17 and CIP4 develop many protrusions simultaneously over the entire surface of individual liposomes, whereas PSTPIP1 and Pacsin2 develop only a few protrusions from a narrow restricted part of the surface of individual liposomes. Tubules formed by FBP17 or CIP4 have higher bending rigidities than those formed by PSTPIP1 or Pacsin2. The results provide striking evidence that these four F-BAR domain family proteins should be classified into two groups: one group of FBP17 and CIP4 and another group of PSTPIP1 and Pacsin2. This classification is consistent with the phylogenetic proximity among these proteins and suggests that the nature of the respective tubulation is associated with biological function. These findings aid in the quantitative assessment with respect to manipulating the morphology of lipid bilayers using membrane-deforming proteins.


Subject(s)
Liposomes/chemistry , Microtubule-Associated Proteins/chemistry , Adaptor Proteins, Signal Transducing/chemistry , Carrier Proteins/chemistry , Chemical Phenomena , Cytoskeletal Proteins/chemistry , Fatty Acid-Binding Proteins , Liposomes/ultrastructure , Microscopy, Fluorescence , Microtubule-Associated Proteins/classification , Minor Histocompatibility Antigens , Models, Biological , Phylogeny
17.
Langmuir ; 27(18): 11528-35, 2011 Sep 20.
Article in English | MEDLINE | ID: mdl-21819144

ABSTRACT

To construct a simple model of a cellular system equipped with motor proteins, cell-sized giant liposomes encapsulating various amounts of actoHMM, the complexes of actin filaments (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), with a depletion reagent to mimic the crowding effect of inside of living cell, were prepared. We adapted the methodology of the spontaneous transfer of water-in-oil (W/O) droplets through a phospholipid monolayer into the bulk aqueous phase and successfully prepared stable giant liposomes encapsulating the solution with a physiological salt concentration containing the desired concentrations of actoHMM, which had been almost impossible to obtain using currently adapted methodologies such as natural swelling and electro-formation on an electrode. We then examined the effect of ATP on the cytoskeleton components confined in those cell-sized liposomes, because ATP is known to drive the sliding motion for actoHMM. We added α-hemolysin, a bacterial membrane pore-forming toxin, to the bathing solution and obtained liposomes with the protein pores embedded on the bilayer membrane to allow the transfer of ATP inside the liposomes. We show that, by the ATP supply, the actoHMM bundles inside the liposomes exhibit specific changes in spatial distribution, caused by the active sliding between F-actin and HMM. Interestingly, all F-actins localized around the inner periphery of liposomes smaller than a critical size, whereas in the bulk solution and also in larger liposomes, the actin bundles formed aster-like structures under the same conditions.


Subject(s)
Actins/metabolism , Liposomes/metabolism , Myosin Subfragments/metabolism , Adenosine Triphosphate/metabolism , Animals , Capsules , Intracellular Space/metabolism , Methylcellulose/metabolism , Protein Binding , Rabbits
18.
Phys Rev E Stat Nonlin Soft Matter Phys ; 81(5 Pt 1): 051921, 2010 May.
Article in English | MEDLINE | ID: mdl-20866275

ABSTRACT

We report the appearance of a spatially segregated state in a microsphere as a result of cooperation between actin filaments and giant DNA molecules. When the coexisting actin concentration is high enough, DNA molecules are excluded toward the surface by forming an assembly with actin filaments. With a decrease in the actin concentration, actin filaments tend to dissolve within the sphere whereas DNA molecules remain to be excluded onto the surface. When the actin concentration becomes still lower, DNA molecules dissolve within the sphere by avoiding surface attachment. We interpret these experimental trends by introducing the concept of an "exclusion zone" for actin filaments within a microsphere.


Subject(s)
Actins/chemistry , Biophysics/methods , DNA/chemistry , Animals , Hydrogen-Ion Concentration , Microscopy, Fluorescence/methods , Microspheres , Models, Biological , Muscle, Skeletal/metabolism , Normal Distribution , Phospholipids/chemistry , Polymers/chemistry , Rabbits
19.
Methods Enzymol ; 464: 31-53, 2009.
Article in English | MEDLINE | ID: mdl-19903549

ABSTRACT

To shed light on the mechanism underlying the active morphogenesis of living cells in relation to the organization of internal cytoskeletal networks, the development of new methodologies to construct artificial cell models is crucial. Here, we describe the successful construction of cell-sized liposomes entrapping cytoskeletal proteins. We discuss experimental protocols to prepare giant liposomes encapsulating desired amounts of actin and cross-linking proteins including molecular motor proteins, such as fascin, alpha-actinin, filamin, myosin-I isolated from brush border (BBMI), and heavy meromyosin (HMM). Subfragment 1 (S-1) is also studied in comparison to HMM, where S-1 and HMM are single-headed and double-headed derivatives of conventional myosin (myosin-II), respectively. In the absence of cross-linking proteins, actin filaments (F-actin) are distributed homogeneously without any order within the liposomes. In contrast, when actin is encapsulated together with an actin-cross-linking protein, mesh structures emerge that are similar to those in living motile cells. Optical microscopic observations on the active morphological changes of the liposomes are reported.


Subject(s)
Actins/metabolism , Liposomes/metabolism , Molecular Biology/methods , Actins/chemistry , Cell Size , Intercalating Agents/chemistry , Intercalating Agents/metabolism , Liposomes/chemical synthesis , Protein Binding
20.
Langmuir ; 25(19): 11680-5, 2009 Oct 06.
Article in English | MEDLINE | ID: mdl-19725557

ABSTRACT

To apply accurate and uniform osmotic pressures to liposomes, they can be formed using the spontaneous transfer method in solutions with different osmolarities. The majority of liposomes unexpectedly opened large holes (several micrometers in diameter) in response to the osmotic pressure regardless of its strength, that is, the difference between the outside and inside solute (sucrose or KCl) concentrations. However, the lag time for any response, including the opening of a hole, after the formation of the liposome decreased with increasing osmotic pressure.


Subject(s)
Liposomes/chemistry , Osmotic Pressure , Potassium Chloride/chemistry , Solutions , Sucrose/chemistry , Time Factors , Water/chemistry
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