ABSTRACT
OBJECTIVE: to determine the impact of the irradiated in vitro blood cells from patients with B-cell chronic lymphocytic leukemia (CLL) on the level of chromosomal instability in peripheral blood lymphocytes (PBL) from healthy persons during the development of tumor-induced bystander effect. MATERIALS AND METHODS: Separate and joint cultivation of PBL from healthy persons (cells-bystanders) together withblood cells from CLL patients irradiated in vitro at the G0 stage of the mitotic cycle by γ-quanta 137Cs in a dose of0.5 Gy 137Cs (cells-inductors) was used. For joint cultivation our own model system for co-cultivation of PBL fromindividuals of different sex, designed by us to investigate the bystander effects at the cytogenetic level was used.Traditional cytogenetic analysis of uniformly painted chromosomes with group karyotyping was performed. The frequency of chromosome aberrations in cells-inductors and cells-bystanders as the markers of chromosome instability were determined. RESULTS: Found that at co-cultivation of PBL from healthy individuals with irradiated blood cells from CLL patientsthe middle group frequency of chromosome aberrations in the bystander cells (5.18 ± 0.51 per 100 metaphases,p < 0.001) was statistically significant higher than its background level determined at a separate cultivaton (1.52± 0.30 per 100 metaphases), and at co-cultivation with non-irradiated blood cells from CLL patients (3.31 ± 0.50 per100 metaphases, p < 0.01). CONCLUSIONS: Co-cultivation of in vitro irradiated blood cells from CLL patients with PBL from healthy persons leadsto an increase in the level of chromosome instability in the bystander cells due to synergism between tumor-inducedand radiation-induced bystander effects.
Subject(s)
Bystander Effect , Chromosomal Instability/radiation effects , Chromosome Aberrations/radiation effects , Leukemia, Lymphocytic, Chronic, B-Cell/genetics , Leukocytes, Mononuclear/immunology , Lymphocytes/radiation effects , Adult , Aged , Cell Communication/genetics , Cell Communication/immunology , Cesium Radioisotopes , Coculture Techniques , Female , Gamma Rays/adverse effects , Healthy Volunteers , Humans , Leukemia, Lymphocytic, Chronic, B-Cell/immunology , Leukemia, Lymphocytic, Chronic, B-Cell/pathology , Leukocytes, Mononuclear/cytology , Lymphocytes/immunology , Lymphocytes/pathology , Male , Metaphase , Middle Aged , Primary Cell CultureABSTRACT
With the help of mixed culture of the irradiated and non-irradiated human peripheral blood lymphocytes received from persons of different gender and G-banding metaphase chromosomes staining the cytogenetic features of induction and persistence of chromosome instability in human lymphocytes as a result of the bystander effect following ionizing radiation exposure both in vitro and in vivo at low and high doses had been established.
Subject(s)
Bystander Effect/radiation effects , Chernobyl Nuclear Accident , Chromosome Aberrations , Lymphocytes/radiation effects , Radiation, Ionizing , Cells, Cultured , Chromosomal Instability/radiation effects , Chromosome Aberrations/radiation effects , Chromosome Aberrations/statistics & numerical data , Coculture Techniques , Cytogenetic Analysis , Dose-Response Relationship, Radiation , Female , Humans , Male , Middle AgedABSTRACT
Results of cytogenetic investigation of the rate and the types of chromosome aberrations in lymphocytes of peripheric blood in 31 infertile women are presented. The high frequency of karyotype abnormalities (16.4%) has been shown in the studied group. Morphofunctional characteristics of the oocytes of the women with normal and abnormal karyotypes have been compared. Oocytes of the patients with chromosome abnormalities were characterized by reduced ability to fertilization and further development.
