ABSTRACT
Investigations were carried out on an influence of phospholipids from L. monocytogenes and A. fumigatus on the degradation of Streptococcus faecalis cell walls by mouse peritoneal macrophages. It has been found, that macrophages from mice stimulated with phospholipids under studies degraded streptococcal cell walls labeled with DL-alanine-I-14C more efficiently than normal macrophages. Activated macrophages degraded also more rapidly antigenic structures of bacterial cell walls reacting with specific fluorescein-labeled antibodies. Moreover, the increase in activity of lysosomal acid hydrolases in stimulated macrophages was observed. The experiments performed suggest that increased ability of macrophages to degradation of bacterial antigens may be connected with a primary augmentation of lysosomal enzyme activity.
Subject(s)
Antigens, Bacterial/immunology , Aspergillus fumigatus/immunology , Listeria monocytogenes/immunology , Macrophages/immunology , Animals , Cell Wall/immunology , Lysosomes/metabolism , Mice , Peritoneal Cavity/cytology , Phagocytosis , Phospholipids/immunology , Streptococcus/immunologyABSTRACT
The effect of cefotaxime and cephradine on the activity of some immune mechanisms was investigated in mice. It was found that cefotaxime in therapeutic doses did not affect the humoral and cellular immune response against sheep erythrocytes, whereas cephradine suppressed the humoral response in doses corresponding to those used for the treatment of patients. The response in vitro of mouse spleen lymphocytes to PHA was suppressed by both cephalosporins; however this occurred at therapeutic concentrations only in the case of cephradine. Neither cephalosporin affected the phagocytic and chemotactic activity of mouse peritoneal macrophages and rabbit microphages.
Subject(s)
Antibody Formation/drug effects , Cefotaxime/pharmacology , Cephalosporins/pharmacology , Cephradine/pharmacology , Immunity, Cellular/drug effects , Spleen/drug effects , Animals , Cefotaxime/immunology , Cells, Cultured , Cephradine/immunology , Hemolysin Proteins/immunology , Hemolytic Plaque Technique , Immunosuppression Therapy , Injections, Intramuscular , Macrophages/drug effects , Macrophages/immunology , Mice , Rabbits , Spleen/immunologyABSTRACT
Investigations were carried out on an influence of phospholipids from A. fumigatus and L. monocytogenes on the bactericidal and fungicidal activity of mouse peritoneal macrophages. L. monocytogenes bacilli and C. neoformans fungi served as the experimental microorganisms. Phospholipids from A. fumigatus and L. monocytogenes were shown to significantly increase macrophage activity under in vivo and in vitro conditions. This was accompanied by an intensified bactericidal and fungicidal activity of extracts from the lysosomal fraction of phagocyte examined. Phospholipid-stimulated macrophages were observed to liberate bactericidal factors into the culture fluid. Lymphocytes had no influence upon this process.
Subject(s)
Aspergillus fumigatus/immunology , Listeria monocytogenes/immunology , Macrophage Activation/drug effects , Phospholipids/pharmacology , Animals , Cytotoxicity, Immunologic/drug effects , In Vitro Techniques , Macrophages/drug effects , Macrophages/immunology , Mice , Phospholipids/immunologyABSTRACT
The administration of phospholipids from Listeria monocytogenes and Aspergillus fumigatus to mice resulted in the development of macrophages with increased functional activity. It has been found that stimulated macrophages have increased phagocytic activity, enhanced chemotactic reactivity and augmented adherence to plastic surface. The activity of FcIg macrophages and complement receptors as well as the rate of the recovery of the FcIg receptors after phagocytosis were significantly higher in activated cells. The increase in phagocytosis, and FcIg and complement receptors activity was observed in macrophages cultured in vitro with phospholipids studied.
