Comparative Study of Inhaled Fluticasone Versus Oral Prednisone in 30 Dogs with Cough and Tracheal Collapse.

Coughing is common in dogs with tracheal collapse (TC). The use of inhaled corticosteroids is less widespread than oral ones. This study aims to compare the effects of oral and inhaled corticosteroids in dogs with cough and TC. Thirty dogs were prospectively included and randomized to the prednisone oral group (OG, 14) or fluticasone inhaled group (IG, 16). A clinical score (CS) based on four clinical parameters (respiratory distress, cough episodes, cough frequency, tracheal sensitivity) was monitored at the hospital (enrolment and weeks 2 and 4). Water intake, urination habits, and adherence and tolerance to treatments were monitored weekly. Significant improvements in clinical parameters were identified in both groups throughout the study. Between-group (OG-IG) comparisons revealed no significant differences, indicating equivalent improvement. At the study's endpoint, the IG dogs had a significantly lower CS (5.69 ± 0.79) than OG dogs (6.43 ± 1.02, p < 0.05). Adherence and tolerance were comparable. From weeks 2 to 4, OG dogs were significantly thirstier and urinated more frequently than IG dogs. In conclusion, fluticasone provided good tolerability and efficacy in controlling cough in dogs with TC, and they showed a lower incidence of signs of hypercortisolism compared to prednisone. These data encourage the use of inhaled fluticasone in dogs with cough and TC.

Evaluation of heart fatty acid-binding protein as a biomarker for canine leishmaniosis.

BACKGROUND: Myocarditis frequently occurs in canine leishmaniosis (CanL). Heart fatty acid-binding protein (HFABP) is a biomarker of myocardial damage. METHODS: This study aimed to compare HFABP concentration (HFABPc) in healthy dogs and dogs at different stages of CanL and evaluate the correlation of this biomarker with several clinicopathological and echocardiographic variables. Thirty-one dogs diagnosed with CanL and 10 healthy dogs were included. RESULTS: HFABPc was not statistically different (p > 0.05) between groups of dogs at different LeishVet stages of CanL or between groups with high versus low to intermediate serology titres. In 70% of CanL dogs, HFABPc was within the 95% confidence interval limits of the mean of healthy dogs. A moderate negative correlation with globulin (r = -0.519; p = 0.03) and haematocrit (HCT) (r = -0.538; p = 0.02) was observed. No other significant correlation (p > 0.05) was observed with any other variable. LIMITATIONS: Many statistical tests were performed, and therefore, type I error cannot be ruled out. CONCLUSION: HFABPc is not consistently elevated in dogs with CanL and is not associated with the severity of the disease, or most echocardiographic or clinicopathological variables studied. The correlation with globulin and HCT was not strong and not considered clinically significant. HFABPc lacks sufficient predictive capacity in dogs with CanL, discouraging further research or clinical use of this biomarker in this disease.

Transthoracic Echocardiographic Examination in the Rabbit Model.

Large animal models such as the rabbit are valuable for translational preclinical research. Rabbits have a similar cardiac electrophysiology compared to that of humans and that of other large animal models such as dogs and pigs. However, the rabbit model has the additional advantage of lower maintenance costs compared to other large animal models. The longitudinal evaluation of cardiac function using echocardiography, when appropriately implemented, is a useful methodology for preclinical assessment of novel therapies for heart failure with reduced ejection fraction (e.g. cardiac regeneration). The correct use of this non-invasive tool requires the implementation of a standardized examination protocol following international guidelines. Here we describe, step by step, a detailed protocol supervised by veterinary cardiologists for performing echocardiography in the rabbit model, and demonstrate how to correctly obtain the different echocardiographic views and imaging planes, as well as the different imaging modes available in a clinical echocardiography system routinely used in human and veterinary patients.

Evaluation of the right parasternal four-chamber view for the assessment of left ventricular longitudinal strain and strain rate by two-dimensional speckle tracking echocardiography in dogs.

Two-dimensional (2-D) speckle tracking echocardiography (STE) is a relatively new imaging technique, introduced in veterinary medicine to aid the assessment of left ventricular (LV) myocardial function. Among other indices, LV longitudinal strain and strain rate (SR) can be measured with this modality, and in dogs they are usually obtained from the left apical four-chamber (LAp4Ch) view. However, in this species, a good quality right parasternal four-chamber (RP4Ch) view can generally be attained, and the aim of this study was therefore to determine its feasibility and reliability for longitudinal strain and SR assessment, and to establish whether the two projections can be used interchangeably. Fifty-one healthy dogs and twenty-five dogs with various cardiac diseases were examined. Longitudinal global and segmental strains and global SR of the LV were obtained with 2-D STE by use of RP4Ch and LAp4Ch views. Intra-observer (within-day and between-day) and inter-observer variabilities were established, and strain and SR values obtained from the two views were compared. The RP4Ch view demonstrated to be feasible for the assessment of longitudinal strain and SR by use of 2-D STE in healthy and diseased dogs. However, out of sector motion of the apical segments could occur and induce tracking errors. The deformation parameters obtained from this view and the LAp4Ch view were often significantly different, and therefore they should not be used interchangeably. Further, the software employed in the present study performed better global than segmental strain analysis for both views.

Percutaneous Contrast Echocardiography-guided Intramyocardial Injection and Cell Delivery in a Large Preclinical Model.

Cell and gene therapy are exciting and promising strategies for the purpose of cardiac regeneration in the setting of heart failure with reduced ejection fraction (HFrEF). Before they can be considered for use, and implemented in humans, extensive preclinical studies are required in large animal models to evaluate the safety, efficacy, and fate of the injectate (e.g., stem cells) once delivered into the myocardium. Small rodent models offer advantages (e.g., cost effectiveness, amenability for genetic manipulation); however, given inherent limitations of these models, the findings in these rarely translate into the clinic. Conversely, large animal models such as rabbits, have advantages (e.g., similar cardiac electrophysiology compared to humans and other large animals), whilst retaining a good cost-effective balance. Here, we demonstrate how to perform a percutaneous contrast echocardiography-guided intramyocardial injection (IMI) technique, which is minimally invasive, safe, well tolerated, and very effective in the targeted delivery of injectates, including cells, into several locations within the myocardium of a rabbit model. For the implementation of this technique, we also have taken advantage of a widely available clinical echocardiography system. After putting in practice the protocol described here, a researcher with basic ultrasound knowledge will become competent in the performance of this versatile and minimally invasive technique for routine use in experiments, aimed at hypothesis testing of the capabilities of cardiac regenerative therapeutics in the rabbit model. Once competency is achieved, the whole procedure can be performed within 25 min after anaesthetizing the rabbit.

Nasopharyngeal stenosis secondary to soft palate dysgenesis in a cat.

This report is the first description of soft palate dysgenesis inducing nasopharyngeal stenosis in a cat. The symptomatology included continuous stertorous respiration and changes in phonation. Open-mouth breathing, seromucous nasal discharge and sneezing were intermittently present. On physical examination the cat showed a respiratory pattern characteristic of upper airway disease, with stridor, increased inspiratory effort and severe bilateral obstruction of nasal passages to the airflow. Pharyngo-laryngoscopic evaluation showed an abnormal conformation of the soft palate that appeared attached to the caudal and lateral pharyngeal wall and stretched the whole length of the pharynx. A strip of soft tissue emanated from the caudal part of the hard palate to the right aspect of the base of the tongue. Caudal to this strip of tissue, the intrapharyngeal opening was stenosed, therefore preventing normal airflow. Surgical reconstruction was not recommended. The cat was clinically stable with mild respiratory symptoms 17 months after the diagnosis.