1.
Prep Biochem
; 18(2): 199-203, 1988.
Article
in English
| MEDLINE
| ID: mdl-3375206
ABSTRACT
The defatted starch was dispersed in NaOH (1 M) and neutralized with HCl (1 M). The amylose 1-butanol complex is adsorbed on defatted cellulose powder in the solvent system containing acetate buffer (pH 4.8,0.1 M) + urea (2 M) + 1-butanol (8.5%, v/v). The complex adsorbed on cellulose powder is separated by centrifugation (2418 g). The sediment is washed with the solvent system-I to obtain the intermediate fraction. The adsorbed amylose is eluted with urea (2 M) in acetate buffer (pH 4.8, 0.1 M). The amylose, intermediate fraction and amylopectin were precipitated with ethanol, washed free of urea and air dried. They were characterized by determining their blue value and beta -amylolysis limit.
Subject(s)
Amylopectin/isolation & purification , Amylose/isolation & purification , Solanum tuberosum/analysis , Starch/analysis , Cellulose
2.
J Chromatogr
; 403: 373-8, 1987 Aug 21.
Article
in English
| MEDLINE
| ID: mdl-3680425