1.
Bioorg Khim
; 35(5): 665-73, 2009.
Article
in Russian
| MEDLINE
| ID: mdl-19915645
ABSTRACT
New approaches to the detection of impaired nucleotides based on the allele specific ligation of a "C probe" followed by rolling circle amplification have been developed. The detection of amplification products was realized by using enzymatic and deoxyribozyme digestion of fluorescently-labeled DNA-RNA-DNA chimeric oligonucleotide structures in cycling probe technology (CPT) in real-time mode.
Subject(s)
Base Pair Mismatch , Fluorescent Dyes/chemistry , Nucleic Acid Amplification Techniques/methods , Oligonucleotide Probes/chemistry
2.
Bioorg Khim
; 34(4): 536-9, 2008.
Article
in Russian
| MEDLINE
| ID: mdl-18695727
ABSTRACT
The interaction of betulin diacetate with formaldehyde by the Prins reaction in various media was studied. As a result, 3beta,28-di-O-acetyl-30-hydroxymethyl-(20)29-lupene, 3beta-acetyl-28-hydroxy-(20)29-lupene, and 3beta,28-di-O-acetoxy-19-(5',6'-dihydro-2'H-pyran-4'-yl)-20,29,30-trinorlupane were obtained.
