ABSTRACT
A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84 g/L were obtained using glucose as the carbon source after 24 h of cultivation in shake flasks. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was synthesized when propionate was provided as secondary carbon source. Salinivibrio sp. TGB10 exhibited favorable tolerance to propionate. The use of 8 g/L propionate and 20 g/L glucose as combinational substrates yielded 1.45 g/L PHBV with a 3-hydroxyvalerate monomer content of 72.02 mol% in flask cultures. In bioreactor study, CDW of 33.45 g/L and PHBV titer of 27.36 g/L were obtained after 108 h of fed-batch cultivation. The results indicated that Salinivibrio sp. TGB10 is a promising halophilic bacterium for the production of PHBV with various polymer compositions.
