ABSTRACT
OBJECTIVE: To observe the improvement effects of puerarin on glycated brain damages in rat model induced by D-galactose. METHOD: The model rats of protein glycation were induced by intraperitoneal administration of D-galactose (150 mg x kg(-1) x d(-1)) for 8 weeks, and all rats were treated with puerarin (high dose 300 mg x kg(-1), middle dose 150 mg x kg(-1), low dose 75 mg x kg(-1)) for 6 weeks. The activity of aldose reductase in red blood cells, the amount of glycated products (fructosamine in serum, glycohaemoglobin, advanced glycation end-products) and AGEs in brain tissue, calcium ion in brain cells were measured. Moreover, mitochondria in brain hippocampus cells were observed under electronic microscope. RESULT: High dose and middle dose of puerarin can decrease the activity of aldose reductase in red blood cells (P < 0.01), and inhibit the formation of glycation products significantly in model rats induced by D-galactose (P < 0.01). Also, puerarin can decrease the content of AGEs in brain and the level of calcium ions in brain cells (P < 0.05, P < 0.01), and decrease lesions degree in mitochondria in brain hippocampus cells. CONCLUSION: Puerarin can produce the protective effects on glycated brain damages through inhibiting the glycation reaction in rats induced by D-galactose.
Subject(s)
Brain/pathology , Isoflavones/pharmacology , Neuroprotective Agents/pharmacology , Pueraria , Aldehyde Reductase/metabolism , Animals , Brain/metabolism , Calcium/metabolism , Erythrocytes/enzymology , Female , Fructosamine/blood , Galactose/antagonists & inhibitors , Glycated Hemoglobin/metabolism , Glycation End Products, Advanced/metabolism , Hippocampus/ultrastructure , Isoflavones/isolation & purification , Male , Mitochondria/ultrastructure , Plants, Medicinal/chemistry , Pueraria/chemistry , Random Allocation , Rats , Rats, Sprague-DawleyABSTRACT
OBJECTIVE: To observe the effects of Radix Puerariae on protein glycation in model rats induced by D-galactose. METHODS: The model rats of protein glycatin were induced by intraperitoneal administration of D-galactose (150 mg/kg) for 8 weeks, and all rats were treated by Radix Puerariae (High dose 300 mg/kg, Middle dose 150 mg/kg, Low dose 75 mg/kg) for 6 weeks. The activity of aldose reductase in red blood cells, the content of fructosamine in serum, the amount of glycohaemoglobin and advanced glycation end-products, the level of insulin in serum, the activity of superoxide dismutase and the amount of maleic dialdehyde were measured. RESULTS: High dose and middle dose of Radix Puerariae could decrease the level of blood glucose and the activity of aldose reductase in red blood cells, inhibit the formation of glycation products significantly in model rats induced by D-galactose (P < 0.01), increase insulin sensitivity and activity of superoxide dismutase (P < 0.01) and decrease the amount of maleic dialdehyde (P < 0.01). CONCLUSION: Puerariae can significantly inhibit glycation reaction in rats induced by D-galactose.
