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1.
Food Chem ; 309: 125654, 2020 Mar 30.
Article in English | MEDLINE | ID: mdl-31678669

ABSTRACT

A specialized DNA extraction method and a SYBR Green quantitative polymerase chain reaction (SyG-qPCR) assay were combined to generate a ready-to-use kit for rapid detection of porcine admixtures in processed meat products. Our qPCR assay utilized repetitive LINE-1 elements specific to the genome of Sus scrofa domesticus (pig) as a target and incorporated internal controls. We improved the genomic DNA extraction method, and reduced extraction times to the minimum. The method was validated for specificity, sensitivity (0.001% w/w) and robustness, and values were compared with those of a commercially available kit. We also tested our method using 121 processed food products and consistently detected amplification only in samples containing pork. Due to its efficiency and cost-effectiveness, our method represents a valuable new method for detecting food adulteration with pork that is superior to existing quality control approaches.


Subject(s)
DNA/analysis , Food Contamination/analysis , Organic Chemicals/chemistry , Real-Time Polymerase Chain Reaction/methods , Animals , Benzothiazoles , DNA/isolation & purification , DNA/standards , Diamines , Long Interspersed Nucleotide Elements/genetics , Meat Products/analysis , Quality Control , Quinolines , Real-Time Polymerase Chain Reaction/instrumentation , Sus scrofa/genetics , Swine
2.
Vet Dermatol ; 29(5): 442-e148, 2018 Oct.
Article in English | MEDLINE | ID: mdl-30066413

ABSTRACT

BACKGROUND: Cutaneous screw-worm myiasis in companion animals either due to the New World screw-worm, Cochliomyia hominivorax or the Old World screw-worm, Chrysomya bezziana, has been reported particularly in tropical and subtropical regions of the world. Although treatment of screw-worm myiasis in dogs has been described, few studies have been conducted regarding its clinical and epidemiological aspects in companion animals. OBJECTIVES: To describe clinical and epidemiological aspects of canine and feline screw-worm myiasis. ANIMALS: Naturally infested dogs and cats, presented to five veterinary clinics in four Malaysian states from September 2017 to February 2018. METHODS AND MATERIALS: Cutaneous screw-worm myiasis was diagnosed based on clinical signs and visual examination of burrowing larvae within lesion. Age, breed, gender, anatomical site of infestation and suspected underlying predisposing causes were investigated. RESULTS: A total of 55 dogs and 21 cats were included in the study. Intact male mixed breed dogs (mean age 58 months) and intact male domestic short hair cats (mean age 24 months) with suspected fight-related wounds were most commonly presented with exudative and ulcerative lesions associated with screw-worm myiasis. The most common anatomical sites of infestation in the dogs were the external ear canals, followed by the perineum and medial canthus. For the cats, the most commonly affected areas were paws and tail. Five cats with screw-worm myiasis were concurrently infected with sporotrichosis. CONCLUSION AND CLINICAL RELEVANCE: Aggression between unneutered animals is a likely underlying cause for cutaneous screw-worm myiasis in both cats and dogs. Sporotrichosis was also a potential predisposing cause for screw-worm myiasis in cats.


Subject(s)
Cat Diseases/parasitology , Dog Diseases/parasitology , Screw Worm Infection/veterinary , Animals , Cat Diseases/epidemiology , Cat Diseases/pathology , Cats , Diptera , Dog Diseases/epidemiology , Dog Diseases/pathology , Dogs , Female , Malaysia/epidemiology , Male , Risk Factors , Screw Worm Infection/epidemiology , Screw Worm Infection/pathology , Skin/parasitology , Skin/pathology
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