[Application progress of innovative high resolution separation and analysis technology in food safety detection field].

In the last decade, mass spectrometry (MS) has been widely used in food inspection science, and the continuous improvement of instruments is the key to the breakthrough of analytical chemistry technology. This paper discusses the latest progress and application of high resolution separation and analysis technology in food safety field (2013-2023), focusing on the innovative development technologies such as on-line MS coupling technology, high resolution screening technology and micro mass spectrometer. Finally, the development of new device in the field of food safety detection is prospected.

Correction: A versatile two-photon fluorescent probe for ratiometric imaging E. coliß-galactosidase in live cells and in vivo.

Correction for 'A versatile two-photon fluorescent probe for ratiometric imaging E. coliß-galactosidase in live cells and in vivo' by Xue-Xiang Zhang et al., Chem. Commun., 2016, 52, 8283-8286.

Changes in collagenous tissue microstructures and distributions of cathepsin L in body wall of autolytic sea cucumber (Stichopus japonicus).

The autolysis of sea cucumber (Stichopus japonicus) was induced by ultraviolet (UV) irradiation, and the changes of microstructures of collagenous tissues and distributions of cathepsin L were investigated using histological and histochemical techniques. Intact collagen fibers in fresh S. japonicus dermis were disaggregated into collagen fibrils after UV stimuli. Cathepsin L was identified inside the surface of vacuoles in the fresh S. japonicus dermis cells. After the UV stimuli, the membranes of vacuoles and cells were fused together, and cathepsin L was released from cells and diffused into tissues. The density of cathepsin L was positively correlated with the speed and degree of autolysis in different layers of body wall. Our results revealed that lysosomal cathepsin L was released from cells in response to UV stimuli, which contacts and degrades the extracellular substrates such as collagen fibers, and thus participates in the autolysis of S. japonicus.

A versatile two-photon fluorescent probe for ratiometric imaging E. coliß-galactosidase in live cells and in vivo.

We have described the design, synthesis, spectroscopy and biological applications of NI-ßGal, a versatile fluorescent probe to detect E. coliß-galactosidase in live cells and mice sensitively and directly, which holds great promise for its application in biomedical research such as gene therapy for cancer in the future.