Radiomics-based analysis of dynamic contrast-enhanced magnetic resonance image: A prediction nomogram for lymphovascular invasion in breast cancer.

OBJECTIVE: To develop and validate a nomogram for quantitively predicting lymphovascular invasion (LVI) of breast cancer (BC) based on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) radiomics and morphological features. METHODS: We retrospectively divided 238 patients with BC into training and validation cohorts. Radiomic features from DCE-MRI were subdivided into A1 and A2, representing the first and second post-contrast images respectively. We utilized the minimal redundancy maximal relevance filter to extract radiomic features, then we employed the least absolute shrinkage and selection operator regression to screen these features and calculate individualized radiomics score (Rad score). Through the application of multivariate logistic regression, we built a prediction nomogram that integrated DCE-MRI radiomics and MR morphological features (MR-MF). The diagnostic capabilities were evaluated by comparing C-indices and calibration curves. RESULTS: The diagnostic efficiency of the A1/A2 radiomics model surpassed that of the A1 and A2 alone. Furthermore, we incorporated the MR-MF (diffusion-weighted imaging rim sign, peritumoral edema) and optimized Radiomics into a hybrid nomogram. The C-indices for the training and validation cohorts were 0.868 (95% CI: 0.839-0.898) and 0.847 (95% CI: 0.787-0.907), respectively, indicating a good level of discrimination. Moreover, the calibration plots demonstrated excellent agreement in the training and validation cohorts, confirming the effectiveness of the calibration. CONCLUSION: This nomogram combined MR-MF and A1/A2 Radiomics has the potential to preoperatively predict LVI in patients with BC.

pH-Dependent Reversible Self-Assembly of ß-Lactoglobulin-Derived Reducing Peptides.

Peptide-based self-assembled nanostructures are emerging vehicles for nutrient delivery and interface engineering. The present study screened eight ß-lactoglobulin (ß-Lg) derived peptides and found that two reducing peptides [EQSLVCQCLV (EV-10) and VCQCLVR (VR-7)] demonstrated pH-dependent reversible fibrilization. EV-10 formed fibrils at pH 2.0 but became unordered aggregates at pH 7.0. VR-7 showed the opposite trend. Both peptides could undergo repetitive transitions between fibrils and unordered aggregates during consecutive pH-cycling. Fibrilization of both peptides was dominated by charges carried by N- and C-terminals. Both fibrils were characterized by a cross-ß sheet structure where the ß-sheet was arranged in an antiparallel manner. Fe3+ was reduced by Cys and EV-10 (pH 5.0 and 7.0) simultaneously upon mixing. In contrast, EV-10 fibrils released Fe3+ reducing capacity progressively, which were beneficial to long-term protection Fe2+. The EV-10 fibrils remained intact after simulated gastric digestion and finally dissociated after intestinal digestion. The results shed light on the mechanisms of fibrilization of ß-Lg derived peptides. This study was beneficial to the rational design of smart pH-responsive materials for drug delivery and antioxidants for nutrients susceptible to oxidation.

Governance of Assisted Living in Long-Term Care: A Systematic Literature Review.

Assisted living (AL) is an emerging model of care in countries where long-term care needs are escalating, with emphasis given to promoting independence and autonomy among the residents to achieve active and healthy ageing. Unlike established nursing homes, the governance of AL is nebulous due to its novelty and diverse nature of operations in many jurisdictions. A comprehensive understanding of how AL is governed globally is important to inform regulatory policies as the adoption of AL increases. A systematic literature review was undertaken to understand the different levels of regulations that need to be instituted to govern AL effectively. A total of 65 studies, conducted between 1990 to 2020, identified from three major databases (PubMed, Medline, and Scopus), were included. Using a thematic synthesis analytical approach, we identified macro-level regulations (operational authorisation, care quality assessment and infrastructural requirements), meso-level regulations (operational management, staff management and distribution, service provision and care monitoring, and crisis management), and micro-level regulations (clear criteria for resident admission and staff hiring) that are important in the governance of AL. Large-scale adoption of AL without compromising the quality, equity and affordability would require clear provisions of micro-, meso- and macro-level regulations.

Stability of Nano-ZnO in simulated landfill leachate containing heavy metal ions.

As the presence of nanosized zinc oxide particles (nano-ZnO) in landfill leachate increases, their interaction with coexisting heavy metal ions (HMs) also increases. The interface interaction between nano-ZnO and HMs will influence nano-ZnO stability and therefore affect its bioavailability and environmental impact. In the present study, we investigated the effects of Cu(II), Cr(III), and Cr(VI) ions on the aggregation, sedimentation, and dissolution of nano-ZnO using batch experiments with a view to better understanding their co-effect on the environment. Dynamic light scattering and UV-Vis spectroscopy results show enhanced aggregation of nano-ZnO in the presence of Cr(VI) ions under fresh landfill leachate conditions, in addition to distinct sedimentation of nano-ZnO in the presence of Cr(III) ions in both fresh and aged landfill leachate. In fresh leachate, Cu(II) ions improved the concentration of dissolved Zn from nano-ZnO. However, the effects of Cu(II), Cr(III), and Cr(VI) ions on the aggregation and dissolution of nano-ZnO were markedly reduced in aged landfill leachate. Both acetic and humic acids in landfill leachate significantly affected the stability of nano-ZnO in the presence of HMs. According to the ATR-FTIR results, Cr(III) ions reacted with hydroxyl groups on nano-ZnO to form ZnO-O bonds, which induced chains of nano-ZnO and Cr(III) complexes, and hence the increased of nano-ZnO aggregates. ATR-FTIR shows merely electrostatic adsorption effects between nano-ZnO and Cu(II) or Cr(VI) ions. In brief, the mode of interactions between HMs and nano-ZnO influenced the stability via adsorption and binding effects. The results of the present research provide insight into the potential effects of nano-ZnO on the environment in the presence of HMs in landfill leachate.

Formation potential of nine nitrosamines from polyacrylamide during chloramination.

Cationic polymers, which are commonly used as flocculants and coagulant aids in water and wastewater treatment, have been recently reported to promote the formation of nitrosamines. Most of the findings to date are based on poly (epichlorohydrin dimethylamine) and poly (diallyldimethylammonium chloride), while few studies have considered nitrosamines formation of polyacrylamides. In this work, the nitrosamines formation from non-ionic, anionic and cationic polyacrylamides was evaluated. Moreover, the effects of chemical structures of cationic polyacrylamides (including molecular weight, charge density, and monomers) on nitrosamines formation were investigated. The results revealed that the highest amount of nitrosamines formation was formed from cationic polyacrylamide, followed by non-ionic polyacrylamide and anionic polyacrylamide. Molecular weight and various cationic monomers showed no significant effects on nitrosamines formation, but monomers generated significantly higher amount of nitrosamines formation than cationic polyacrylamides. Nitrosamines formation increased with the increasing charge density of cationic polyacrylamides, and FTIR analysis results showed that the quaternary amine groups preferentially reacted with chloramines than with amide groups. This work shed new light on the nitrosamines formation from water and wastewater treatment polymers.

Factors affecting N-nitrosodimethylamine formation from poly(diallyldimethyl-ammonium chloride) degradation during chloramination.

Poly(diallyldimethylammonium chloride) (polyDADMAC) has been shown to be an important precursor of the probable human carcinogen N-nitrosodimethylamine (NDMA) when in contact with chloramine. In this study, we conducted an orthogonal experiment design to evaluate the effects of pH values, ammonia, bromide, natural organic matter (NOM) and monochloramine dosages on the formation of NDMA from polyDADMAC during chloramination. Meanwhile, single-factor experiments of pH, bromide and NOM prove the results of orthogonal experiment. The results supported that pH was the most critical factor affecting NDMA formation from polyDADMAC during chloramination, and the highest NDMA formation from polyDADMAC occurred at pH near 7 due to released DMA from polyDADMAC degradation and the critical importance of low concentrations of dichloramine in water. In the presence of excess bromide, the NDMA formation was enhanced significantly at all different pH values owing to bromochloramine, which has higher electronegativity of the brominated nitrogen atom than monochloramine or dichloramine. The NDMA formation from polyDADMAC in the presence of NOM was 41.7% lower than NDMA formation in the absence of NOM. The overwhelming majority of NDMA formation from polyDADMAC under simulated conditions was lower than the current advisory levels (i.e. 9 ng l-1 in Ontario, 10 ng l-1 in California).