Iron-rich digestate biochar toward sustainable peroxymonosulfate activation for efficient anaerobic digestate dewaterability.

In this study, a suite of Fe-rich biochars derived from Fenton-like treated digestate (Fe-BC) were fabricated under different pyrolysis temperatures (300, 500, and 800 °C), which were firstly utilized as peroxymonosulfate (PMS) activators for promoting digestate dewaterability with wide applicability. Results showed that compared to the Fe-BC300/Fe-BC500 + PMS treatments, Fe-BC800 + PMS process performed superior digestate dewaterability in which specific resistance to filtration reduction and water content reduction improved by > 12.5% and > 130%, respectively, under the optimal conditions. Mechanistic results demonstrated that in Fe-BC800 + PMS system, HO• and SO4•- oxidation played a pivotal role on promoted digestate dewaterability, while HO• and 1O2 oxidation was dominated in Fe-BC300/Fe-BC500 + PMS treatments. Fe-BC800 containing higher Fe and CO contents could efficiently interact with PMS to generate numerous HO• and SO4•- via iron cycle. These highly reactive oxygen species proficiently reduced the hydrophilic biopolymers, protein molecules, and amino acids in extracellular polymeric substances, leading to remarkable decrease in particle size, hydrophilicity, adhesion, network strength, and bound water of digestate. Consequently, the flowability and dewaterability of digestate could be significantly enhanced. The cost-benefit result indicated the Fe-BC + PMS treatment possessed desirable reusability, applicability, and economic viability. Collectively, the Fe-BC + PMS is a high-performance and eco-friendly technique for digestate dewatering, which opens a new horizon towards a closed-loop of digestate reutilization.

Simultaneous nitrogen and phosphorus removal by interactions between phosphate accumulating organisms (PAOs) and denitrifying phosphate accumulating organisms (DPAOs) in a sequencing batch reactor.

The identification of phosphate-accumulating organisms (PAOs), denitrifying phosphate-accumulating organisms (DPAOs) and their relationship is a key pathway for optimizing nitrate and phosphate removal efficiency in activated sludge. In this study, the acclimatization of microorganisms in sequencing batch reactor were performed with anaerobic/aerobic (A/O) and anaerobic/anoxic (A/A) cycles, the biomass changes of PAOs and DPAOs and the correlations were then discussed. The results indicated that after acclimatization, the nutrient removal efficiencies reached to 85.34% (COD), 93.64% (PO43--P) and 92.34% (NO3--N), respectively, with NO3--N:PO43-P of 1.5:1. The successful enrichment of PAOs and DPAOs (reached 97.9%) was verified by the change of relative metabolic activities, which was further proved by the change of bacterial diversity. The number of Candidatus Accumulibacter, Zoogloea, and Dechloromonas all increased at A/O and A/A stages while the number of Acinetobacter only increased at A/O stage. So Accumulibacter sp. was DPAO while Acinetobacter sp. was only PAO in this process, and genera Accumulibacter, Dechloromonas and Zoogloea greatly coordinated in denitrification and accumulating phosphorous though RDA and chord plot. This was worthy of attention and development to explore enhanced biological phosphorus removal (EBPR) in practical wastewater treatment via improving identification of bacterial species and symbiosis of bacteria community.