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1.
Sensors (Basel) ; 19(7)2019 Apr 11.
Article in English | MEDLINE | ID: mdl-30978941

ABSTRACT

Testing for blood-transmitted infectious agents is an important aspect of safe medical treatment. During emergencies, such as significant earthquakes, many patients need surgical treatment and/or blood transfusion. Because a waveguide mode (WM) sensor can be used as a portable, on-site blood testing device in emergency settings, we have previously developed WM sensors for detection of antibodies against hepatitis B virus and hepatitis C virus and for forward ABO and Rh(D) and reverse ABO blood typing. In this study, we compared signal enhancement methods using secondary antibodies conjugated with peroxidase, a fluorescent dye, and gold nanoparticles, and found that the peroxidase reaction method offers superior sensitivity while gold nanoparticles provide the most rapid detection of anti-HBs antibody. Next, we examined whether we could apply a WM sensor with signal enhancement with peroxidase or gold nanoparticles to detection of antibodies against hepatitis C virus, human immunodeficiency virus and Treponema pallidum, and HBs antigen in plasma. We showed that a WM sensor can detect significant signals of these infectious agents within 30 min. Therefore, a portable device utilizing a WM sensor can be used for on-site blood testing of infectious agents in emergency settings.


Subject(s)
Biosensing Techniques , HIV/isolation & purification , Hepacivirus/isolation & purification , Hepatitis B virus/isolation & purification , Treponema pallidum/isolation & purification , ABO Blood-Group System/blood , ABO Blood-Group System/isolation & purification , Antibodies/blood , Antibodies/isolation & purification , Gold/chemistry , HIV/pathogenicity , HIV Infections/blood , Hepacivirus/pathogenicity , Hepatitis B virus/pathogenicity , Hepatitis C/blood , Humans , Metal Nanoparticles/chemistry , Syphilis/blood , Syphilis/microbiology , Treponema pallidum/pathogenicity
2.
J Biosci Bioeng ; 126(1): 131-137, 2018 Jul.
Article in English | MEDLINE | ID: mdl-29499994

ABSTRACT

Portable, on-site blood typing methods will help provide life-saving blood transfusions to patients during an emergency or natural calamity, such as significant earthquakes. We have previously developed waveguide-mode (WM) sensors for forward ABO and Rh(D) blood typing and detection of antibodies against hepatitis B virus and hepatitis C virus. In this study, we evaluated a WM-sensor for reverse ABO blood typing. Since reverse ABO blood typing is a method for detection of antibodies against type A and type B oligosaccharide antigens on the surface of red blood cells (RBCs), we fixed a synthetic type A or type B trisaccharide antigen on the sensor chip of the WM sensor. We obtained significant changes in the reflectance spectra from a WM sensor on type A antigen with type B plasma and type O plasma and on type B antigen with type A plasma and type O plasma, and no spectrum changes on type A antigen or type B antigen with type AB plasma. Signal enhancement with the addition of a peroxidase reaction failed to increase the sensitivity for detection on oligosaccharide chips. By utilizing hemagglutination detection using regent type A and type B RBCs, we successfully determined reverse ABO blood groups with higher sensitivity compared to a method using oligosaccharide antigens. Thus, functionality of a portable device utilizing a WM sensor can be expanded to include reverse ABO blood typing and, in combination with forward ABO typing and antivirus antibody detection, may be useful for on-site blood testing in emergency settings.


Subject(s)
ABO Blood-Group System/analysis , Biosensing Techniques/methods , Blood Grouping and Crossmatching/methods , ABO Blood-Group System/blood , ABO Blood-Group System/immunology , Adult , Biosensing Techniques/standards , Blood Grouping and Crossmatching/standards , Erythrocytes/cytology , Erythrocytes/immunology , Female , Hemagglutination/physiology , Hemagglutination Tests/methods , Hemagglutination Tests/standards , Humans , Male , Sensitivity and Specificity , Trisaccharides/analysis , Trisaccharides/blood
3.
J Biosci Bioeng ; 123(6): 760-764, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28190739

ABSTRACT

In large-scale disasters, such as huge significant earthquakes, on-site examination for blood typing and infectious disease screening will be very helpful to save lives of victims who need surgical treatment and/or blood transfusion. However, physical damage, such as building collapse, electric power failure and traffic blockage, disrupts the capacity of the medical system. Portable diagnostic devices are useful in such cases of emergency. In this study, we evaluated a waveguide-mode sensor for detection of anti-hepatitis virus antibodies. First, we examined whether we can detect antigen-antibody interaction on a sensor chip immobilized hepatitis B virus surface (HBs) antigen and hepatitis C virus (HCV) core antigen using monoclonal mouse antibodies for HBs antigen and HCV core antigen. We obtained significant changes in the reflectance spectra, which indicate specific antigen-antibody interaction for anti-HBs antibody and anti-HCV antibody. Next, we examined the effect of horseradish peroxidase-conjugated secondary antibody using aminoethyl carbazole as the peroxidase substrate and found that the colorimetric reaction increases detection sensitivity for anti-HBs antibody more than 300 times. Finally, we successfully detected anti-HBs antibody in human blood samples with an enhancing method using a peroxidase reaction. Thus, a portable device utilizing a waveguide-mode sensor may be applied to on-site blood testing in emergency settings.


Subject(s)
Antibodies, Viral/blood , Antibodies, Viral/immunology , Biosensing Techniques/methods , Hepatitis B Core Antigens/immunology , Hepatitis B Surface Antigens/immunology , Blood Transfusion , Humans
4.
J Biosci Bioeng ; 116(5): 620-3, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23742748

ABSTRACT

A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.


Subject(s)
Hepacivirus/chemistry , Lipids , Proteins/chemistry , Proteins/metabolism , Cell Line , Cell Survival , Humans , Membrane Proteins/metabolism , Microscopy, Confocal , Perilipin-2 , Protein Structure, Tertiary , Proteins/genetics , Substrate Specificity , Time Factors , Viral Nonstructural Proteins/chemistry
5.
J Ethnopharmacol ; 146(3): 866-72, 2013 Apr 19.
Article in English | MEDLINE | ID: mdl-23439031

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: The fruits of Chaenomeles sinensis Koehne (Chinese quince) are distributed throughout China and Japan. It has traditionally been known to have a therapeutic effect against respiratory symptoms caused by infectious diseases. AIM OF THE STUDY: The polyphenol-rich extract, CSD3, from Chaenomeles sinensis has previously been shown to neutralize influenza virus infectivity. The aim of this study was to clarify which step(s) in the replication cycle in vitro were inhibited. MATERIALS AND METHODS: We examined cell-binding, hemagglutination and hemolytic activities and infectivity of A/Udorn/72(H3N2) virus after pre-treatment with CSD3. We also investigated the time course of synthesis for viral mRNA, cRNA, and vRNA in Madin-Darby canine kidney epithelial cells (MDCK) cells infected with CSD3-treated virus. Finally, we studied the effect of CSD3-treatment on the ultrastructure of the influenza virion. RESULTS: Pre-treatment with CSD3 mildly reduced cell-binding, hemagglutination and hemolytic activities. These activities were reduced by 70% to be equivalent to 30% of the control at 1µg/ml. CSD3 severely reduced infectivity to 1% of the control at 1µg/ml. Primary transcription in MDCK cells infected with CSD3 (1µg/ml)-treated virus was decreased to about 1% of that in cells infected with mock-treated virus. Synthesis of viral cRNA, vRNA and secondary mRNA was also severely decreased. Electron microscopy revealed that the integrity of the virus envelope was damaged by CSD3 and was permeable to uranyl acetate. CONCLUSIONS: The main target step(s) of CSD3 in the replication cycle is after cell-binding but before or at primary transcription. Involvement of the increased permeability of virus envelope as the inhibition mechanism was proposed. CSD3 could be useful in preventing influenza virus infection, and be employed as a lozenge or mouthwash for daily use.


Subject(s)
Antiviral Agents/pharmacology , Drugs, Chinese Herbal/pharmacology , Influenza A Virus, H3N2 Subtype/drug effects , Polyphenols/pharmacology , Rosaceae/chemistry , Transcription, Genetic/drug effects , Animals , Antiviral Agents/isolation & purification , Cell Culture Techniques , Chickens , Dogs , Drugs, Chinese Herbal/isolation & purification , Epithelial Cells/drug effects , Epithelial Cells/virology , Erythrocytes/drug effects , Erythrocytes/virology , Hemagglutination, Viral/drug effects , Hemolysis/drug effects , Influenza A Virus, H3N2 Subtype/genetics , Influenza A Virus, H3N2 Subtype/ultrastructure , Madin Darby Canine Kidney Cells , Microscopy, Electron, Transmission , Polyphenols/isolation & purification , RNA, Viral/biosynthesis , RNA, Viral/genetics , Virion/ultrastructure , Virus Replication/drug effects
6.
J Lipid Res ; 54(4): 881-92, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23315449

ABSTRACT

Lipid droplets (LD) are dynamic storage organelles that are involved in lipid homeostasis. Hepatitis C virus (HCV) is closely associated with LDs. HCV Core and nonstructural (NS) proteins colocalize with LDs and presumably are involved in virion formation at that site. We demonstrated that HCV NS4B, an integral membrane protein in endoplasmic reticulum (ER), strongly targeted LDs. Confocal imaging studies showed that NS4B localized at the margins of LDs. Biochemical fractionation of HCV-replicating cells suggested that NS4B existed in membranes associated with LDs rather than on the LD surface membrane itself. The N- and C-terminal cytosolic domains of NS4B showed targeting of LDs, with the former being much stronger. In both domains, activity was present in the region containing an amphipathic α-helix, in which 10 hydrophobic residues were identified as putative determinants for targeting LDs. JFH1 mutants with alanine substitutions for the hydrophobic residues were defective for virus replication. W43A mutant with a single alanine substitution showed loss of association of NS4B with LDs and severely reduced release of infectious virions compared with wild-type JFH1. NS4B plays a crucial role in virus replication at the site of virion formation, namely, the microenvironment associated with LDs.


Subject(s)
Hepacivirus/metabolism , Organelles/metabolism , Viral Nonstructural Proteins/chemistry , Viral Nonstructural Proteins/metabolism , Cell Line , Endoplasmic Reticulum/metabolism , Fluorescent Antibody Technique , Hepacivirus/physiology , Humans , Hydrophobic and Hydrophilic Interactions , Immunoblotting , Viral Nonstructural Proteins/genetics
7.
PLoS One ; 7(9): e45371, 2012.
Article in English | MEDLINE | ID: mdl-23028967

ABSTRACT

Influenza virus neuraminidase (NA) cleaves terminal sialic acid residues on oligosaccharide chains that are receptors for virus binding, thus playing an important role in the release of virions from infected cells to promote the spread of cell-to-cell infection. In addition, NA plays a role at the initial stage of viral infection in the respiratory tract by degrading hemagglutination inhibitors in body fluid which competitively inhibit receptor binding of the virus. Current first line anti-influenza drugs are viral NA-specific inhibitors, which do not inhibit bacterial neuraminidases. Since neuraminidase producing bacteria have been isolated from oral and upper respiratory commensal bacterial flora, we posited that bacterial neuraminidases could decrease the antiviral effectiveness of NA inhibitor drugs in respiratory organs when viral NA is inhibited. Using in vitro models of infection, we aimed to clarify the effects of bacterial neuraminidases on influenza virus infection in the presence of the NA inhibitor drug zanamivir. We found that zanamivir reduced progeny virus yield to less than 2% of that in its absence, however the yield was restored almost entirely by the exogenous addition of bacterial neuraminidase from Streptococcus pneumoniae. Furthermore, cell-to-cell infection was severely inhibited by zanamivir but restored by the addition of bacterial neuraminidase. Next we examined the effects of bacterial neuraminidase on hemagglutination inhibition and infectivity neutralization activities of human saliva in the presence of zanamivir. We found that the drug enhanced both inhibitory activities of saliva, while the addition of bacterial neuraminidase diminished this enhancement. Altogether, our results showed that bacterial neuraminidases functioned as the predominant NA when viral NA was inhibited to promote the spread of infection and to inactivate the neutralization activity of saliva. We propose that neuraminidase from bacterial flora in patients may reduce the efficacy of NA inhibitor drugs during influenza virus infection. (295 words).


Subject(s)
Antiviral Agents/pharmacology , Bacterial Proteins/pharmacology , Enzyme Inhibitors/pharmacology , Neuraminidase/antagonists & inhibitors , Neuraminidase/pharmacology , Orthomyxoviridae/drug effects , Virus Replication/drug effects , Animals , Cell Line , Dogs , Streptococcus pneumoniae/enzymology , Viral Proteins/antagonists & inhibitors , Viral Proteins/metabolism , Zanamivir/pharmacology
8.
J Biosci Bioeng ; 110(3): 374-6, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20547361

ABSTRACT

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.


Subject(s)
Antigens/chemistry , Detergents/chemistry , Recombinant Proteins/chemistry , Single-Chain Antibodies/biosynthesis , Single-Chain Antibodies/chemistry , Single-Chain Antibodies/genetics , Solubility
9.
J Virol Methods ; 156(1-2): 162-5, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19027795

ABSTRACT

The distribution of the matrix (M1) protein of influenza virus in infected cells was examined using immunostaining. The fixation method influenced strongly the immunofluorescence pattern of the M1 protein. The M1 protein was distributed uniformly in both the cytoplasm and in nuclei when cells that had been infected with virus were fixed with paraformaldehyde. In cells that had been fixed with methanol, however, nuclear dots of the M1 protein were clearly visible. The dots were evident at 8h post-inoculation. Up to 6h post-inoculation, only a diffuse distribution of the M1 protein was observed. The dots were co-localized with promyelocytic leukemia (PML) protein, a major component of nuclear domain 10 (ND10), also called PML oncogenic domains (PODs) or PML-nuclear bodies (NBs). These results indicate that the nuclear dots of the M1 protein in cells that had been fixed with methanol are not artifacts of the fixation method. Furthermore, methanol fixation is preferred for localization of the influenza M1 protein in nuclei using immunostaining.


Subject(s)
Fixatives/chemistry , Influenza A virus/chemistry , Viral Matrix Proteins/analysis , Animals , Cell Line , Fluorescent Antibody Technique , Formaldehyde/chemistry , Methanol/chemistry , Polymers/chemistry
10.
Biochem Biophys Res Commun ; 362(4): 880-5, 2007 Nov 03.
Article in English | MEDLINE | ID: mdl-17767916

ABSTRACT

Influenza A virus non-structural protein 1 (NS1) is the most important viral regulatory factor that controls cellular processes to facilitate viral replication. To gain further insight into the role of NS1, we tried to find novel cellular factors that interact with NS1. The complexes of NS1 and target proteins were pulled down from an infected cell lysate using anti-NS1 (A/Udorn/72) single-chain Fv and identified by peptide mass fingerprinting analysis. We identified nucleolin, a multifunctional major nucleolar protein, as a novel NS1-binding protein. The RNA-binding domain of NS1 was responsible for this binding, as judged by a GST (glutathione S-transferase) pull-down assay with the GST-fused functional domains of NS1. By laser confocal microscopy, we observed the co-localization of NS1 with nucleolin most clearly in the nucleoli, indicating that NS1 is interacting with nucleolin during infection. Our results suggest a novel function of NS1, namely, affecting cellular events via interaction with nucleolin.


Subject(s)
Influenza A virus/physiology , Influenza, Human/metabolism , Influenza, Human/virology , Lung Neoplasms/metabolism , Lung Neoplasms/virology , Phosphoproteins/metabolism , RNA-Binding Proteins/metabolism , Viral Nonstructural Proteins/metabolism , Cell Line, Tumor , Humans , Nucleolin
11.
Hepatol Res ; 37(2): 113-20, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17300706

ABSTRACT

AIM: With advent of reverse-transcription (RT)/polymerase chain reaction (PCR) for detection of the hepatitis E viral genome, we carried out retrospective examinations. METHODS: Serum samples collected from 68 patients diagnosed as viral hepatitis with unknown etiology were tested for viral markers of hepatitis virus. RESULTS: Two of them were found positive for hepatitis E viral RNA. While the clinical course of one patient (patient 1) was typical as acute hepatitis E, another patient (patient 2) was persistently infected with HEV. Patient 2 was infected with the virus via blood transfusion during chemotherapy against T-cell lymphoma. The entire viral genome from the donor was identical with that from the serum of patient 2 obtained on day 170 after the transfusion of the implicated red blood cell (RBC) product, confirming the transmission of HEV by transfusion. The patient remained negative for anti-HEV antibodies for the follow-up period of six months, probably due to immune suppression by lymphoma and chemotherapy. CONCLUSION: We report here an unusual case of long-term HEV infection in a patient with T-cell lymphoma. Persistent infection with HEV was probably due to the absence of anti-HEV antibodies, which was caused by lymphoma and chemotherapy.

12.
Genes Cells ; 12(1): 75-88, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17212656

ABSTRACT

Dystroglycan (DG) is a widely expressed, transmembrane glycoprotein complex that plays important roles by connecting the extracellular matrix to the cytoskeleton. The alpha- and beta-DG subunits are produced by the cleavage of residues 653 and 654 of the precursor. To clarify the mechanisms involved in cleavage and subunit association, we performed a series of mutation analyses and made the following discoveries: (i) Disruption of the intramolecular disulfide bridge between Cys669 and Cys713 in beta-DG completely abolishes the cleavage, (ii) deletions in the loop region (669-713) and in the C-terminal region of alpha-DG (550-645) abolish the cleavage, (iii) disruption of the disulfide bridge and deletions in the loop region deteriorate the alpha- and beta-DG subunit association, and (iv) at the cleavage site, especially, positions P1' (Ser654) and P6' (Trp659) are critical. Thus, the critical role of the Cys669-Cys713 disulfide bridge formation is, most likely, to form a specific tertiary structure, in which the alpha- and beta-DG domains interact and the cleavage site becomes susceptible to proteolytic reactions. The Cys669 and Cys713 pair is broadly conserved in vertebrates and in some invertebrates, suggesting that the disulfide bridge formation was established early in the evolution of DG.


Subject(s)
Cysteine/chemistry , Disulfides/chemistry , Dystroglycans/chemistry , Dystroglycans/metabolism , Protein Subunits/chemistry , Protein Subunits/metabolism , Amino Acid Sequence , Animals , CHO Cells , Cells, Cultured , Cricetinae , Cricetulus , Cysteine/metabolism , Humans , Models, Biological , Molecular Sequence Data , Transfection
14.
J Biochem ; 132(2): 245-51, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12153722

ABSTRACT

We describe a novel method, two-dimensional electrophoresis/phage panning (2D-PP), for the generation of antibodies against proteins in crude biochemical samples, such as cellular membrane fractions. These sources have traditionally presented problems as to the development of antibodies by conventional techniques. 2D-PP involves two-dimensional resolution of proteins, blotting of the proteins onto a nitrocellulose membrane, and screening of a phage antibody library and isolation of corresponding antibodies. By 2D-PP with detergent-insoluble "lipid rafts" as a target protein complex, we obtained specific phage pools against eight antigen spots (from a total of 39 spots). These antibodies were functional in Western blotting, enzyme-linked immunosorbent assaying (ELISA), and immunoscreening of a cDNA expression library. Propagation of anti-nitrocellulose phages was the major problem in 2D-PP, but was overcome by the use of the soluble anti-nitrocellulose antibody fragment. 2D-PP constitutes a key tool for functional analysis of proteins in complex fractions.


Subject(s)
Antibodies/immunology , Antibodies/isolation & purification , Bacteriophages/metabolism , Electrophoresis, Gel, Two-Dimensional/methods , Immunoblotting/methods , Proteins/isolation & purification , Antibodies/metabolism , Detergents/chemistry , Enzyme-Linked Immunosorbent Assay , Humans , Hydrogen-Ion Concentration , Membrane Microdomains/chemistry , Membrane Microdomains/metabolism , Peptide Library , Proteins/immunology , Proteins/metabolism , Tumor Cells, Cultured
15.
J Biol Chem ; 277(33): 30382-7, 2002 Aug 16.
Article in English | MEDLINE | ID: mdl-12048201

ABSTRACT

We have established a novel method, in situ phage screening (ISPS), to identify proteins in tissue microstructures. The method is based on the selection of repertoires of phage-displayed antibody fragments with small samples of tissues microdissected using a laser. Using a human muscle frozen section with an area of 4800 microm2 as a model target, we successfully selected monoclonal antibody fragments directed against three major (myosin heavy chain, actin, and tropomyosin-alpha) and one minor (alpha-actinin 2) muscle constituent proteins. These proteins were present in the sample in amounts less than one nanogram, and the antibodies were used to visualize the proteins in situ. This shows that the use of ISPS can obtain monoclonal antibodies for histochemical and biochemical purposes against minute amounts of proteins from microstructures with no requirement for large amounts of samples or biochemical efforts.


Subject(s)
Bacteriophages/genetics , Immunoglobulin Fragments/genetics , Antigens/genetics , Base Sequence , DNA Primers , Humans , Muscle, Skeletal/metabolism
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