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Science ; 302(5646): 842-6, 2003 Oct 31.
Article in English | MEDLINE | ID: mdl-14593172

ABSTRACT

Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.


Subject(s)
Arabidopsis/genetics , Genome, Plant , RNA, Messenger/genetics , RNA, Plant/genetics , Transcription, Genetic , Chromosome Mapping , Chromosomes, Plant/genetics , Cloning, Molecular , Computational Biology , DNA, Complementary/genetics , DNA, Intergenic , Expressed Sequence Tags , Gene Expression Profiling , Genes, Plant , Genomics , Nucleic Acid Hybridization , Oligonucleotide Array Sequence Analysis , Open Reading Frames , Reverse Transcriptase Polymerase Chain Reaction
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