[Study on the malignant and normal rectum tissues using 1H NMR spectroscopy].

Cancer is one of the most serious diseases, a threat to human body's health and a causes of death. The early diagnosis of cancer and timely therapy is significant for improving the survival. Owing to the complexity and limit of conventional medical diagnosis, misdiagnosis of ten occurs in many cases. NMR spectroscopy is an effective technique for characterizing molecular structure and component changes. The component and structural information of nucleic acid, protein, lipid and glucide in the biologic tissues can also be distinguished from NMR data. In the present paper, a new method was developed for the diagnosis of tumor using the advanced physical chemistry (NMR) and biomedicine technique. Nine rectum tissue samples and their corresponding normal tissues of rectum were measured using NMR spectroscopy. Each tissue sample obtained from the operation was quickly separated into two parts averagely: one was dipped into 10% formalin solution and prepared for conventional pathological examination; the other was preserved in liquid nitrogen for further NMR detection. Before NMR detection, the corresponding sample was thawed at room temperature and was dipped into 0.5 mL D2O. Dioxane was used as external reference. The obtained NMR spectra were analyzed and compared by OMNIC5.0 and SPSS 11.0 Software. The differences of metabolite in the tissues samples were studied and a method for the diagnosis of cancer using NMR data was initially explored. The result indicates that the 1H NMR spectra of rectum cancer tissues are significantly different from those of their corresponding normal rectum tissues. This is shown by the differences in the integral area ratio at characteristic peak region. A0.9/A3.0, A1.3/A3.0, A2.0/ A3.0, A1.3/A0.9 and A4.1/A3.0 of the normal rectum tissues are higher than those of their corresponding cancer tissues, but A3.2/A3.0 is lower than that of the rectum cancer tissues. From the above result it is concluded that the concentration of fatty acid, inositol and lactate is lower in the rectum cancer tissues than in the normal tissues, while that of choline bases compounds is higher. The NMR judgments were also consistent with the pathological examination results. So the NMR spectra may be developed into a method of early diagnosing rectum cancer through these differences.

[Study on the mechanism of fluorescence quenching of pyridine by bisazafulleroid[60] derivative and its electrochemical property].

The mechanism of the fluorescence quenching of pyridine by bisazafulleroid[60] derivative (eddy2) was discussed. When the C60 and eddy2 were added into pyridine respectively, the intensity of 426.27 nm emission peak of pyridine decreased obviously and shifted towards shorter wavelength, a typical quenching phenomenon. The fluorescence quenching of eddy2-pyridine is caused by the static quenching process resulting from the formation of charge-translated complex between molecules. The dissociation constant of eddy2 from pyridine is K(D) = 2.28 x 10(-6) (mol x L(-1)). The combination constant of eddy2 is Ks = 4.39 x 10(-5) (L x mol(-1)). The electrochemical properties of eddy2 were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) on a glassy carbon electrode in a mixed solvent of toluene and acetonitrile. The first three reduction waves for eddy2 shift to more positive than that for C60, indicating the electroreduction of eddy2 is easier than that of C60.

The first trisaza-bridged [60]fulleroid: drilling a hole on the fullerene.

[reaction: see text] Two types of trisaza-bridged [60]fulleroids have been synthesized for the first time. By means of (13)C NMR, (1)H NMR, MALDI-TOF MS, FTIR, UV-vis, and 2D-NMR (gHSQC, gHMBC, and NOSEY) spectroscopy, they have been shown to be tris[6,5]-opened-aza-bridged adducts with substituents on the same five-membered ring with C(s)() symmetry. Some data of nonlinear optics and electrochemistry for the product are also reported.