ABSTRACT
This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been regretfully retracted at the request of the authors due to mistakes which occurred during the figure preparation. Although the authors published a corrigendum about these mistakes (Cancer Lett. 2016 Aug 1. pii: S0304-3835(16)30443-8), they now feel that it is more appropriate to retract the paper to keep their research at a high standard. All authors have agreed to this decision and apologize for any inconvenience caused by retraction of this article.
Subject(s)
Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism , Carcinoma, Non-Small-Cell Lung/metabolism , Lung Neoplasms/metabolism , NF-kappa B/metabolism , Signal Transduction/physiology , Animals , Apoptosis/physiology , Blotting, Western , Female , Humans , Immunoprecipitation , Mice , Mice, Nude , Real-Time Polymerase Chain Reaction , Xenograft Model Antitumor AssaysABSTRACT
Adiponectin plays an important role in improving insulin resistance and preventing atherosclerosis. However it has been rarely reported that adiponectin influences insulin secretion because its receptor was identified in human islet beta cells. In order to investigate the direct effect of adiponectin on pancreatic islet beta cells, we performed an insulin secretion test in purified rat islets, which were incubated with adiponectin (100 ng/mL) at low (3.3 mM) and high (16.7 mM) glucose concentrations. Furthermore, cell lysates were extracted from the adiponectin-treated islets for p-AMPKalpha assay. RTPCR and immunohistochemical examination showed both adiponectin receptor 1 (AdipoR1) and receptor 2 (AdipoR2) were expressed in islet cells and AdipoR1 was predominantly expressed. Insulin secretion was significantly increased in the presence of adiponectin for 6 h at high glucose concentration. Meanwhile, the levels of phosphorylated AMPK increased with adiponectin treatment at high glucose concentrations. It is concluded that adiponectin augments insulin secretion from pancreatic islet beta cells at high glucose concentration through AMPK activation.
