Evidence for endothelial junctions acting as a fluid flux pathway in tensioned periodontal ligament.

A continuous tension load of 1.0 N applied to the rat maxillary first molar for 30 min led to a significant increase (p < 0.05) in the mean number of junctions/microns of endothelial perimeter. The mean number of junctions/microns was compared with the mean number of tissue channels/microns 2 as previously reported by fitting linear and quadratic equations. An increase in the mean number of junctions/microns was associated with an increase in mean number of tissue channels/microns 2 (p < 0.01) in control and experimental periodontal ligament. Significant increases in the mean number of junctions/microns occurred in the middle-third zone for venous capillaries (p < 0.01) and in the tooth-third zone for postcapillary-sized venules (p < 0.001). It is concluded that the endothelial junctions of venous capillaries and postcapillary-sized venules provide significant pathways for fluid transport across the tensioned walls of the microvascular bed of the periodontal ligament.

A TEM analysis of tissue channels in normal and orthodontically tensioned rat molar periodontal ligament.

This investigation tested the hypotheses that (1) tissue channels (TC) exist in normal periodontal ligament (PDL) between the cervical and apical zones, and (2) orthodontic tooth tension alters their number and distribution within this region. A continuous 1.0 N tension was applied to the maxillary first molar for 30 minutes and ionic tracer precipitate used to analyse TC in unstained TEM sections. In normal PDL, the mean number of tissue channels (MNTC) was 0.10 +/- 0.01/micron 2 in the 0-2-microns PDL zone extending from the vessel wall, 0.09 +/- 0.02/micron 2 in the 2-4-microns zone, and 0.06 +/- 0.02/micron 2 in the 4-6 microns zone. Differences were not statistically significant. Extrusion increased the MNTC to 0.28 +/- 0.01/micron 2, 0.20 +/- 0.02/micron 2, and 0.16 +/- 0.02/micron 2, in the 0-2-, 2-4- and 4-6-microns zones, respectively (P < 0.001). For normal PDL in the tooth, middle and bone circumferential thirds the TC distribution were even. In the tensioned PDL, TC in the circumferential tooth third (0.20 +/- 0.02/micron 2), middle third (0.19 +/- 0.01/micron 2), and bone third (0.23 +/- 0.02/micron 2) increased significantly (P < 0.001) from normal ligament. The total increase in experimental MNTC was associated mainly with the post-capillary-sized venules, venous capillaries, and arterial capillaries. With progression from the cervical to apical regions the MNTC in control PDL failed to significantly fit a quadratic regression pattern. By contrast, tensioned PDL showed a marked regression pattern (P < 0.001) indicating a reduction in MNTC with vertical depth.(ABSTRACT TRUNCATED AT 250 WORDS)

An in vitro method for comparing the effects of different root canal preparation techniques on the shape of curved root canals.

This study introduced an in vitro model which was designed to observe the effects of instrumentation on the shape of curved root canals. The model may be accurately rotated through 90 degrees allowing reproducible radiographs to be taken from two different angles. The root canals were filled with a radiopaque contrast medium before radiographs were taken. Two-dimensional evaluation of the shape of the root canals, before and after preparation was made on enlarged photographic prints obtained from the radiographs. A custom-made grid was used as a guide for measurement and accurate transfer from the pre- to postpreparation prints. Reproducibility of measurement was within 4 per cent. This method is feasible for quantitative comparison of the effect of instrumentation on the shape of root canals.

The effect of hand, sonic and ultrasonic instrumentation on the shape of curved root canals.

This study compared the effects of hand, sonic and ultrasonic instrumentation on the shape of curved root canals in 60 extracted human lateral incisors and premolars. Each root of moderate or severe canal curvature was mounted onto a simulated crown and filled with a radiocontrast medium. Enlarged photographic prints obtained from radiographs taken before and after root canal preparation were measured. The results indicated a lower incidence of apical zip and elbow formation in the ultrasonic preparations when compared with those prepared by hand and sonic, irrespective of the canal curvature. A more prominent hour-glass shape was prepared by hand and sonic instruments particularly in severely curved root canals. A distinctive taper was produced by each method of preparation. All three instrumentation techniques showed preferential dentine removal from the convex aspect of the canal in the apical portion and the concave aspect in the middle portion of the root canal.