Response and self-regulation of PD/A granular sludge to oxytetracycline stress.

This paper investigated the operational characteristics and self-regulation mechanism of the partial denitrification/anammox (PD/A) granular system under the stress of oxytetracycline (OTC), an emerging pollutant that accumulates in municipal wastewater treatment plants through various pathways, posing significant challenges for its future promotion in engineering applications. The results indicated that OTC concentrations below 100 mg/L intensified its short-term inhibition on the PD/A granular sludge system, decreasing functional bacterial activity, while between 150 and 300 mg/L, PD's NO3--N to NO2--N conversion ability diminished, and Anammox activity was significantly suppressed. Under long-term high OTC stress (20-30 mg/L), nitrogen removal suffered, and batch tests revealed significant inhibition of PD's NO3--N to NO2--N conversion, dropping from 73.77 % to 50.17 %. Anammox bacteria activity sharply declined from 1.81 to 0.39 mg N/gVSS/h under OTC stress. Extracellular polymeric substances (EPS) content rose from 185.39 to 210.86 mg/gVSS, indicating PD/A sludge's self-protection mechanism. However, EPS content fell due to cell lysis at high OTC (30 mg/L). The decreasing relative abundance of Candidatus_Brocadia (2.32 % to 0.93 %) and Thaure (12.63 % to 7.82 %) was a key factor in the gradual deterioration of denitrification performance. This study was expected to provide guidance for the PD/A process to cope with the interference of antibiotics and other emerging pollutants (short-term shock and long-term stress).

Investigating cultivation strategies for enhancing protein content in Auxenochlorella pyrenoidosa FACHB-5.

This study investigated the influence of yeast extract addition, carbon source, and photoperiod on the growth dynamics of Auxenochlorella pyrenoidosa FACHB-5. Employing response surface methodology, the culture strategy was optimized, resulting in the following optimal conditions: yeast extract addition at 0.75 g L-1, glucose concentration of 0.83 g L-1, and a photoperiod set at Light: Dark = 18 h: 6 h. Under these conditions, the biomass reached 1.76 g L-1 with a protein content of 750.00 g L-1, containing 40 % of essential amino acids, representing a 1.52-fold increase. Proteomic analysis revealed that the targeted cultivation strategy up-regulated genes involved in microalgal protein synthesis. The combined effect of yeast extract and glucose enhanced both the glutamine synthetase-glutamate synthetase mechanism and the free amino acid content.

Suitability of inorganic coagulants for algae-laden water treatment: Trade-off between algae removal and cell viability, aggregate properties and coagulant residue.

Inorganic coagulants could effectively precipitate algae cells but might increase the potential risks of cell damage and coagulant residue. This study was conducted to critically investigate the suitability of polyaluminum (PAC), FeCl3 and TiCl4 for algae-laden water treatment in terms of the trade-off between algal substance removal, cell viability, and coagulant residue. The results showed that an appropriate increase in coagulant dosage contributed to better coagulation performance but severe cell damage and a higher risk of intracellular organic matter (IOM) release. TiCl4 was the most destructive, resulting in 60.85% of the algal cells presenting membrane damage after coagulation. Intense hydrolysis reaction of Ti salts was favorable for the formation of larger and more elongated, dendritic structured flocs than Al and Fe coagulants. TiCl4 exhibited the lowest residue level and remained in the effluents mainly in colloidal form. The study also identified charge neutralization, chemisorption, enmeshment, and complexation as the dominant mechanisms for algae water coagulation by metal coagulants. Overall, this study provides the trade-off analyses between maximizing algae substance removal and minimizing potential damage to cell integrity and is practically valuable to develop the most suitable and feasible technique for algae-laden water treatment.

The effect of quorum sensing on cadmium- and lead-containing wastewater treatment using activated sludge: Removal efficiency, enzyme activity, and microbial community.

Quorum sensing (QS) is prevalent in activated sludge processes; however, its essential role in the treatment of heavy metal wastewater has rarely been studied. Therefore, in this study, acyl homoserine lactone (AHL)-mediated QS was used to regulate the removal performance, enzyme activity, and microbial community of Cd- and Pb-containing wastewater in a sequencing batch reactor (SBR) over 30 cycles. The results showed that exogenous AHL strengthened the removal of Cd(II) and Pb(II) in their coexistence wastewater during the entire period. The removal of NH4+-N, total phosphorus, and chemical oxygen demand (COD) was also enhanced by the addition of AHL despite the coexistence of Cd(II) and Pb(II). Meanwhile, the protein content of extracellular polymeric substances was elevated and the microbial metabolism and antioxidative response were stimulated by the addition of AHL, which was beneficial for resistance to heavy metal stress and promoted pollutant removal by activated sludge. Microbial sequencing indicated that AHL optimized the microbial community structure, with the abundance of dominant taxa Proteobacteria and Unclassified_f_Enterobacteriaceae increasing by 73.9% and 59.2% maximally, respectively. This study offers valuable insights into the mechanisms underlying Cd(II) and Pb(II) removal as well as microbial community succession under AHL availability in industrial wastewater.

Toward nitrogen recovery: Co-cultivation of microalgae and bacteria enhances the production of high-value nitrogen-rich cyanophycin.

The algal-bacterial wastewater treatment process has been proven to be highly efficient in removing nutrients and recovering nitrogen (N). However, the recovery of the valuable N-rich biopolymer, cyanophycin, remains limited. This research explored the synthesis mechanism and recovery potential of cyanophycin within two algal-bacterial symbiotic reactors. The findings reveal that the synergy between algae and bacteria enhances the removal of N and phosphorus. The crude contents of cyanophycin in the algal-bacterial consortia reached 115 and 124 mg/g of mixed liquor suspended solids (MLSS), respectively, showing an increase of 11.7 %-20.4 % (p < 0.001) compared with conventional activated sludge. Among the 170 metagenome-assembled genomes (MAGs) analyzed, 50 were capable of synthesizing cyanophycin, indicating that cyanophycin producers are common in algal-bacterial systems. The compositions of cyanophycin producers in the two algal-bacterial reactors were affected by different lighting initiation time. The study identified two intracellular synthesis pathways for cyanophycin. Approximately 36 MAGs can synthesize cyanophycin de novo using ammonium and glucose, while the remaining 14 MAGs require exogenous arginine for production. Notably, several MAGs with high abundance are capable of assimilating both nitrate and ammonium into cyanophycin, demonstrating a robust N utilization capability. This research also marks the first identification of potential horizontal gene transfer of the cyanophycin synthase encoding gene (cphA) within the wastewater microbial community. This suggests that the spread of cphA could expand the population of cyanophycin producers. The study offers new insights into recycling the high-value N-rich biopolymer cyanophycin, contributing to the advancement of wastewater resource utilization.

l-Cysteine and barium titanate co-modified enteromorpha biochar as effective peroxymonosulfate activator for atrazine treatment.

In this study, pyrolysis and hydrothermal methods were used for Enteromorpha biochar that was co-modified with l-cysteine and barium titanate (LBCBa). It has great environmental tolerance and can remove 93.0 % of atrazine (ATZ, 10 mg·L-1) within 60 mins of ultrasonic treatment. The enhanced hydrophilicity, electron-donating capability, and piezoelectricity of LBCBa are considered to induce excellent performance. The apparent reaction rate of the LBCBa-2/PMS/ATZ system with ultrasonic was 2.87 times that without ultrasonic. The density functional theory points out that, introducing l-cysteine to carbon edges improves the adsorption of ATZ and peroxymonosulfate (PMS), making PMS easier to activate. This work offered unique insights for fabricating effective catalysts and demonstrated the combination of hydrophilic functional groups and piezoelectricity in improving catalytic performance and stability.

MOF-derived Fe/Ni@C marigold-like nanosheets as heterogeneous electro-Fenton cathode for efficient antibiotic oxytetracycline degradation.

The widespread occurrence of organic antibiotic pollution in the environment and the associated harmful effects necessitate effective treatment method. Heterogeneous electro-Fenton (hetero-EF) has been regarded as one of the most promising techniques towards organic pollutant removal. However, the preparation of efficient cathode still remains challenging. Herein, a novel metal-organic framework (MOF)-derived Fe/Ni@C marigold-like nanosheets were fabricated successfully for the degradation of oxytetracycline (OTC) by serving as the hetero-EF cathode. The FeNi3@C (Fe/Ni molar ratio of 1:3) based hetero-EF system exhibited 8.2 times faster OTC removal rate than that of anodic oxidation and possessed many advantages such as excellent OTC degradation efficiency (95.4% within 90 min), broad environmental adaptability (satisfactory treatment performance for multiple antibiotics under various actual water matrixes), good stability and reusability, and significant toxicity reduction. The superior hetero-EF catalytic performance was mainly attributed to: 1) porous carbon and Ni existence were both conducive to the in-situ generation of H2O2 from dissolved O2; 2) the synergistic effects of bimetals together with electron transfer from the cathode promoted the regeneration of ≡ FeII/NiII, thereby accelerating the production of reactive oxygen species; 3) the unique nanosheet structure derived from the precursor two-dimensional Fe-Ni MOFs enhanced the accessibility of active sites. This work presented a promising hetero-EF cathode for the electrocatalytic treatment of antibiotic-containing wastewaters.

Refining microbial potentiometric sensor performance with unique cathodic catalytic properties for targeted application scenarios.

Traditional microbial electrochemical sensors encounter challenges due to their inherent complexity. In response to these challenges, the microbial potentiometric sensor (MPS) technology was introduced, featuring a straightforward high-impedance measurement circuit tailored for environmental monitoring. Nonetheless, the practical implementation of conventional MPS is constrained by issues such as the exposure of the reference electrode to the monitored water and the absence of methodologies to stimulate microbial metabolism. In this study, our objective was to enhance MPS performance by imbuing it with unique cathodic catalytic properties, specifically tailored for distinct application scenarios. Notably, the anodic region served as the sensing element, with both the cathodic region and reference electrode physically isolated from the analyzed water sample. In the realm of organic monitoring, the sensor without Pt/C coated in the cathodic region exhibited a faster response time (1 h) and lower detection limits (1 mg L-1 BOD, 1 mM acetic acid). Conversely, when monitoring toxic substances, the sensor with Pt/C showcased a lower detection limit (0.004% formaldehyde), while the Pt/C-free sensor demonstrated superior reusability. The sensor with Pt/C displayed a heightened anode biofilm thickness and coverage, predominantly composed of Rhodococcus. In conclusion, this study introduces simple, cost-effective, and tailorable biosensors holding substantial promise for water quality monitoring.

Electrochemically selective ammonium recovery from wastewater via coupling hydrogen bonding and charge storage.

Electrochemical ammonium (NH4+) storage (EAS) has been established as an efficient technology for NH4+ recovery from wastewater. However, there are scientific difficulties unsolved regarding low storage capacity and selectivity, restricting its extensive engineering applications. In this work, electrochemically selective NH4+ recovery from wastewater was achieved by coupling hydrogen bonding and charge storage with self-assembled bi-layer composite electrode (GO/V2O5). The NH4+ storage was as high as 234.7 mg N g-1 (> 102 times higher than conventional activated carbon). Three chains of proof were furnished to elucidate the intrinsic mechanisms for such superior performance. Density functional theory (DFT) showed that an excellent electron-donating ability for NH4+ (0.08) and decrease of diffusion barrier (22.3 %) facilitated NH4+ diffusion onto electrode interface. Physio- and electro-chemical results indicated that an increase of interlamellar spacing (14.3 %) and electrochemical active surface area (ECSA, 388.9 %) after the introduction of GO were responsible for providing greater channels and sites toward NH4+ insertion. Both non-ionic chemical-bonding (V5+=O‧‧‧H, hydrogen-bonding) and charge storage were contributed to the higher capacity and selectivity for NH4+. This work offers underlying guideline for exploitation a storage manner for NH4+ recovery from wastewater.

Insights into the rapid start-up of an inoculated municipal sludge system: A high-height-to-diameter-ratio airlift inner-circulation partition bioreactor based on CFD analysis.

The utilization of municipal sludge as a seed sludge for initiating the autotrophic nitrogen removal (ANR) process presents a challenge due to the negligible abundance of anaerobic ammonia-oxidizing bacteria (AnAOB). Here, a computational fluid dynamics model was used to simulate sludge volume fraction and sludge particle velocity. A high-height-to-diameter-ratio airlift inner-circulation partition bioreactor (HHAIPBR) was operated for 175 d to enrich AnAOB from municipal sludge, and the performance of the ANR process was investigated. The start-up period of HHAIPBR inoculated with municipal sludge required approximately 69 d. A high nitrogen removal performance, with a mean total nitrogen removal efficiency of 82.1%, was obtained for 1 month. The simulation results validated the presence of sludge circulation and revealed the distribution characteristics of dissolved oxygen inside the reactor, further supporting the promotion of sludge granulation via the high height-to-diameter ratio. Nitrosomonas (3.31%) of Proteobacteria and Candidatus Brocadia (6.56%) of Planctomycetota were dominant in the HHAIPBR. This study presents a viable approach for the industrial cultivation of anammox sludge and the rapid start-up of the partial nitritation-anammox system.

Effects of biochar layer position on treatment performance and microbial community in subsurface flow constructed wetlands for removal of cadmium and lead.

Levels of cadmium (Cd) and lead (Pb) correspond to common composition in acid mine wastewater of Hunan Province of China. The removal path of Cd and Pb and the structure of microbial community were investigated by developing constructed wetlands (CWs) with different layer positions of biochar. The biochar as a layer at the bottom of CW (BCW) system exhibited maximum Cd and Pb removal efficiencies of 96.6-98.6% and 97.2-98.9%, respectively. Compared with original soil, BCW increased the relative proportions of Proteobacteria, Firmicutes, Acidobacteriota, Verrucomicrobiota, Desulfobacterota, Armatimonadota, Bacteroidota, Patescibacteria, Basidiomycota (phylum level) and Burkholderia-Caballeronia-Paraburkholderia, Citrifermentans, Chthonomonadales, Cellulomonas, Geothrix, Terracidiphilus, Gallionellaceae, Microbacterium, Vanrija, Apiotrichum, Saitozyma, Fusarium (genus level). The concentrations of Cd and Pb were positively correlated with the abundance of Verrucomicrobiota, Basidiomycota (phylum level), and Methylacidiphilaceae, Meyerozyma, Vanrija (genus level). This study demonstrates that BCW system can improve removal performance toward Cd and Pb, as well as alter microbial community.

Robust rehabilitation of anammox system by granular activated carbon under long-term starvation stress: Microbiota restoration and metabolic reinforcement.

This article investigates the buffering capacity and recovery-enhancing ability of granular activated carbon (GAC) in a starved (influent total nitrogen: 20 mg/L) anaerobic ammonium oxidation (anammox) reactor. The findings revealed that anammox aggregated and sustained basal metabolism with shorter performance recovery lag (6 days) and better nitrogen removal efficiency (84.9 %) due to weak electron-repulsion and abundance redox-active groups on GAC's surface. GAC-supported enhanced extracellular polymeric substance secretion aided anammox in resisting starvation. GAC also facilitated anammox bacterial proliferation and expedited the restoration of anammox microbial community from a starved state to its initial-level. Metabolic function analyses unveiled that GAC improved the expression of genes involved in amino acid metabolism and sugar-nucleotide biosynthesis while promoted microbial cross-feeding, ultimately indicating the superior potential of GAC in stimulating more diverse metabolic networks in nutrient-depleted anammox consortia. This research sheds light on the microbial and metabolic mechanisms underlying GAC-mediated anammox system in low-substrate habitats.

Relative contribution of ammonia-oxidizing bacteria and denitrifying fungi to N2O production during rice straw composting with biochar and biogas residue amendments.

Nitrous oxide (N2O) production is associated with ammonia-oxidizing bacteria (amoA-AOB) and denitrifying fungi (nirK-fungi) during the incorporation of biochar and biogas residue composting. This research examined the relative contribution of alterations in the abundance, diversity and structure of amoA-AOB and nirK-fungi communities on N2O emission by real-time PCR and sequence processing. Results showed that N2O emissions showed an extreme relation with the abundance of amoA-AOB (rs = 0.584) while giving credit to nirK-fungi (rs = 0.500). Nitrosomonas and Nitrosospira emerged as the dominant genera driving ammoxidation process. Biogas residue changed the community structure of AOB by altering Nitrosomonadaceae proportion and physiological capacity. The denitrification process, primarily governed by nirK-fungi, served as a crucial pathway for N2O production, unveiling the pivotal mechanism of biochar to suppress N2O emissions. C/N and NH4+-N were identified as significant parameters influencing the distribution of nirK-fungi, especially Micromonospora, Halomonas and Mesorhizobium.

Recent Advances in Thallium Removal from Water Environment by Metal Oxide Material.

Thallium is widely used in industrial and agricultural development. However, there is still a lack of systematic understanding of its environmental hazards and related treatment methods or technologies. Here, we critically assess the environmental behavior of thallium in aqueous systems. In addition, we first discuss the benefits and limitations of the synthetic methods of metal oxide materials that may affect the practicality and scalability of TI removal from water. We then assess the feasibility of different metal oxide materials for TI removal from water by estimating the material properties and contaminant removal mechanisms of four metal oxides (Mn, Fe, Al, and Ti). Next, we discuss the environmental factors that may inhibit the practicality and scalability of Tl removal from water. We conclude by highlighting the materials and processes that could serve as more sustainable alternatives to TI removal with further research and development.

Synergistic effect of intercalation and EDLC electrosorption of 2D/3D interconnected architectures to boost capacitive deionization for water desalination via MoSe2/mesoporous carbon hollow spheres.

Transition-metal dichalcogenides can be used for capacitive deionization (CDI) via pseudocapacitive ion intercalation/de-intercalation due to their unique two-dimensional (2D) laminar structure. MoS2 has been extensively studied in the hybrid capacitive deionization (HCDI), but the desalination performance of MoS2-based electrodes remains only 20-35 mg g-1 on average. Benefiting from the higher conductivity and larger layer spacing of MoSe2 than MoS2, it is expected that MoSe2 would exhibit a superior HCDI desalination performance. Herein, for the first time, we explored the use of MoSe2 in HCDI and synthesized a novel MoSe2/MCHS composite material by utilizing mesoporous carbon hollow spheres (MCHS) as the growth substrate to inhibit the aggregation and improve the conductivity of MoSe2. The as-obtained MoSe2/MCHS presented unique 2D/3D interconnected architectures, allowing for synergistic effects of intercalation pseudocapacitance and electrical double layer capacitance (EDLC). An excellent salt adsorption capacity of 45.25 mg g- 1 and a high salt removal rate of 7.75 mg g- 1 min-1 were achieved in 500 mg L- 1 NaCl feed solution at an applied voltage of 1.2 V in batch-mode tests. Moreover, the MoSe2/MCHS electrode exhibited outstanding cycling performance and low energy consumption, making it suitable for practical applications. This work demonstrates the promising application of selenides in CDI and provides new insights for ration design of high-performance composite electrode materials.

Recent advances and prospects in electrochemical coupling technologies for metal recovery from water.

With the development of our society, the desire to recover valuable metal resources from metal-containing wastewaters or natural water bodies is becoming increasingly stronger nowadays. To overcome the limitations of single techniques, coupling technologies with synergistic effects are attracting increasing attention regarding metal resource recovery from water with particular interest in electrochemical coupling technologies in view of the advantages of electrochemical methods. This state-of-the-art review comprehensively presented the mechanisms and performance of electrochemical coupling systems for metal recovery from water. To give a clear overview of current research trends, technologies coupled with electrochemical processes can be categorized into six main types: electrochemical techniques, membrane modules, adsorption/extraction techniques, sonication technologies, energy supply techniques and others. The electrochemical coupling system has shown synergistic advantages (e.g., improving metal recovery efficiency, reducing energy consumption) over single technologies. We then discuss the remaining challenges, present corresponding solutions, and put forward future directions for current electrochemical coupled systems towards metal recovery. This review is conducive to broadening the potential applications of electrochemical coupling processes for metal recovery and sustainable water treatment.

Ferrate modified carbon felt as excellent heterogeneous electro-Fenton cathode for chloramphenicol degradation.

In this study, a novel and efficient heterogeneous electro-Fenton (EF) process with a potassium ferrate (K2FeO4) modified carbon felt (Fe-CF) cathode was developed for chloramphenicol (CAP) removal. The catalytic activity was assessed by the comparison of different systems and the effects of multiple operating parameters (K2FeO4 dosage, initial solution pH, applied current) and co-existing constituents. Results indicated that the Fe-CF cathode exhibited excellent performance for CAP degradation (almost 100% removal efficiency within 60 min) over a wide range of pH (pH 3-9) during heterogeneous EF ascribed to the synergistic effect of embedded iron species and porous graphitic carbon structure and effective utilization of the in-situ generated H2O2. Moreover, the Fe-CF cathode possessed good recyclability with low metal leaching (98.2% CAP removal efficiency after reused for 5 times) and outstanding real water application performance. The ∙OH and O2∙- were responsible for CAP degradation, while ∙OH played a main role. Moreover, the toxicity evaluation by E. coli growth experiments demonstrated an efficient toxicity reduction in this system. Overall, a novel heterogeneous EF functional cathode with superior performance was fabricated via a green, low-cost one-step method, which shows promising application potential for actual wastewater treatment.

Carbon nanotube-based materials for persulfate activation to degrade organic contaminants: Properties, mechanisms and modification insights.

Removal of harmful organic matters from environment has great environmental significance. Carbon nanotube (CNT) materials and their composites have been demonstrated to possess excellent catalytic activity towards persulfate (PS) activation for the degradation of organic contaminants. Herein, detailed information concerning the function, modification methods and relevant mechanisms of CNT in persulfate-based advanced oxidation processes (PS-AOPs) for organic pollutant elimination has been reviewed. The activation mechanism of PS by CNT might include radical and nonradical pathways and their synergistic effects. The common strategies to improve the stability and catalytic capability of CNT-based materials have also been put forward. Furthermore, their practical application potential compared with other catalysts has been described. Finally, the challenges faced by CNT in practical application are clearly highlighted. This review should be of value in promoting the research of PS activation by CNT-based materials for degradation of organic pollutants and the corresponding practical applications.

Effective fluoride removal from brackish groundwaters by flow-electrode capacitive deionization (FCDI) under a continuous-flow mode.

Herein, we demonstrated the suitability and effectiveness of utilizing flow-electrode capacitive deionization (FCDI) for treatment of fluoride-contaminated brackish groundwater. By comparing operational modes of short-circuited closed-cycle (SCC), isolated closed-cycle (ICC) and single cycle (SC), it was found that SCC mode was the most advantageous. In SCC configuration, the effects of different parameters on the removal of F- and Cl- were investigated including current density, hydraulic residence time (HRT), activated carbon (AC) loading and feed concentration of coexisting NaCl. Results indicated that the steady-state effluent Cl- concentration dropped with elevated applied current, and the decreasing rate got faster with the increase of HRT or AC loading. However, for the steady-state effluent F- concentration, it dropped to a value under a small applied current and maintained stable in spite of the increase in applied current, and both HRT and AC loading had insignificant effects on the steady-state effluent F- concentration. F- was preferentially removed from the treated water compared with Cl-, and a higher ion selectivity could be obtained at lower applied current and smaller HRT with the trade-off being that operation under these circumstances would generate outlet water with little change in conductivity compared to the influent. The removal efficiencies of F- and Cl- both decreased with increasing feed concentration of coexisting NaCl. This study should be of value in establishing FCDI as a viable technology for treatment of fluoride-contaminated brackish groundwater.

The combined toxicity and mechanism of multi-walled carbon nanotubes and nano zinc oxide toward the cabbage.

The natural environment is a complex system, and there is never only one kind of nanomaterial entering the environment. However, many studies only considered the plant toxicity of one kind of nanomaterial and do not consider the influence of two or more kinds of nanomaterials on plant toxicity. Multi-walled carbon nanotubes (MWCNTs) and zinc oxide nanoparticles (ZnO NPs) are two common and widely used nanomaterials in water environment, so these two kinds of nanomaterials were chosen to explore the effects of their combined toxicity on cabbage. This study investigated the toxicity of MWCNTs combined with ZnO NPs on cabbage by measuring the length of roots and stems, chlorophyll content, oxidative stress, antioxidant enzyme activity, metal element content, and root scanning electron microscopy. The toxicity of single MWCNTs toward cabbage was attributed to direct oxidative damage, while the toxicity of single ZnO NPs toward cabbage was due to the high level of zinc concentration. Moreover, ZnO NPs (10 mg/L) ameliorated MWCNTs toxicity toward cabbage by improving the activity of antioxidant enzymes. ZnO NPs (50 and 100 mg/L) because of the high content of zinc disrupted the balance of other metals in the plant and increased the toxicity of MWCNTs. In conclusion, the combined toxicity of different concentrations and types of nanomaterials should be considered for a more accurate assessment of environmental risks.