Dispersed Nickel Phthalocyanine Molecules on Carbon Nanotubes as Cathode Catalysts for Li-CO2 Batteries.

The Li-CO2 battery has great potential for both CO2 utilization and energy storage, but its practical application is limited by low energy efficiency and short cycle life. Efficient cathode catalysts are needed to address this issue. Herein, this work reports on molecularly dispersed electrocatalysts (MDEs) of nickel phthalocyanine (NiPc) anchored on carbon nanotubes (CNTs) as the cathode catalyst for Li-CO2 batteries. The dispersed NiPc molecules efficiently catalyze CO2 reduction, while the conductive and porous CNTs networks facilitate CO2 evolution reaction, leading to enhanced discharging and charging performance compared to the NiPc and CNTs mixture. Octa-cyano substitution on NiPc (NiPc-CN) further enhances the interaction between the molecule and CNTs, resulting in better cycling stability. The Li-CO2 battery with the NiPc-CN MDE cathode shows a high discharge voltage of 2.72 V and a small discharging-charging potential gap of 1.4 V, and can work stably for over 120 cycles. The reversibility of the cathode is confirmed by experimental characterizations. This work lays a foundation for the development of molecular catalysts for Li-CO2 battery cathodes.

Inflammatory mediators and immune function in different stages of systemic lupus erythematosus.

This study aimed to study the relationship between the expression levels of inflammatory mediators IL-36ß and IL-36R and disease symptoms, laboratory indices and somatic immune function in Systemic Lupus Erythematosus (SLE) of different stages. In this research 70 patients with SLE who were treated in public hospitals from February 2020 to December 2021 were randomly divided into the stable group (n=35) and active group (n=35), and serum IL-36 was measured in the two groups ß and IL-36R concentration with the standard curve of Enzyme-Linked Immunosorbent Assay (ELISA) to analyze IL-36ß and IL-36R concentrations. 36ß and IL-36R concentrations were analyzed in relation to the Disease activity score of systemic lupus erythematosus (SLEDAI), disease duration, typical symptoms of SLE and experimental characteristics. Results showed that the differences in IL-36ß and IL-36R concentrations between the stable and active groups overall and for each disease duration group were tiny. There was no significant correlation between serum IL-36ß and IL-36R concentrations and SLEDAI scores in stable and active patients, and a negative correlation between them and disease duration. Serum inflammatory mediator IL-36R concentrations were significantly higher in patients with mucosal ulcers and the difference was statistically significant. differences in IL-36ß concentrations were statistically significant only for indicators of decreased erythrocyte count and IL-36R concentrations were statistically significant for indicators of decreased erythrocyte count, decreased haemoglobin and decreased lymphocytes The differences were huge and tiny in C4 decline, anti-dsDNA, and urinary routine protein. There was a significant positive correlation between IL-36ß and IL-36R concentrations in patients with stable and active SLE, with correlation coefficients of 0.448 and 0.452 respectively. The differences in IL-36ß and IL-36R concentrations between the stable and active groups were tiny for patients in the stable and active groups as a whole and for all disease groups. The differences in the number of each inflammatory mediator positive cells in the epidermal stratum corneum and superficial dermis between patients in the stable and active groups were tiny. In conclusion, IL-36ß and IL-36R proteins in SLE patients are expressed in immune cells as well as epithelial cells of patients, indicating that these two inflammatory mediators may be one of the early signals that activate the immune system of SLE patients and trigger the immune response of patients; the onset of SLE may be associated with the inflammatory response induced by IL-36ß and IL-36R.

Deciphering the selectivity descriptors of heterogeneous metal phthalocyanine electrocatalysts for hydrogen peroxide production.

The electrocatalytic 2e- oxygen reduction reaction (2e- ORR) provides an appealing pathway to produce hydrogen peroxide (H2O2) in a decentralized and clean manner, which drives the demand for developing high selectivity electrocatalysts. However, current understanding on selectivity descriptors of 2e- ORR electrocatalysts is still insufficient, limiting the optimization of catalyst design. Here we study the catalytic performances of a series of metal phthalocyanines (MPcs, M = Co, Ni, Zn, Cu, Mn) for 2e- ORR by combining density functional theory calculations with electrochemical measurements. Two descriptors (ΔG *O - ΔG *OOH and ΔG *H2O2 ) are uncovered for manipulating the selectivity of H2O2 production. ΔG *O - ΔG *OOH reflects the preference of O-O bond breaking of *OOH, affecting the intrinsic selectivities. Due to the high value of ΔG *O - ΔG *OOH, the molecularly dispersed electrocatalyst (MDE) of ZnPc on carbon nanotubes exhibits high selectivity, even superior to the previously reported NiPc MDE. ΔG *H2O2 determines the possibility of further H2O2 reduction to affect the measured selectivities. Enhancing the hydrophobicity of the catalytic layer can increase ΔG *H2O2 , leading to selectivity improvement, especially under high H2O2 production rates. In the gas diffusion electrode measurements, both ZnPc and CoPc MDEs with polytetrafluoroethylene (PTFE) exhibit low overpotentials, high selectivities, and good stability. This study provides guidelines for rational design of 2e- ORR electrocatalysts.

Bortezomib Inhibits Multiple Myeloma Cells by Transactivating ATF3 to Trigger miR-135a-5p- Dependent Apoptosis.

Multiple myeloma (MM) is a malignant cancer with an increasing in incidence that can be alleviated through bortezomib (BTZ) treatment. Activating transcription factor 3 (ATF3) plays a major role in cancer development. Moreover, microRNAs (miRNAs) regulate carcinogenic pathways, apoptosis, and programmed necrotic cell death. However, the detailed mechanism by which ATF3 modulates BTZ drug sensitivity/resistance remains elusive. In the current study, expression of ATF3 was significantly increased under BTZ treatment in a dose-dependent manner in MM cell lines. In addition, ATF3 could regulate cell apoptosis under BTZ treatment. The effect of ATF3 was negatively regulated by its binding miRNA, miR-135a-5p. When either ATF3 was silenced or miR-135a-5p mimics were added to MM cells, they partially lost sensitivity to BTZ treatment. This was accompanied by low levels of Noxa, CHOP, and DR5, and a decrease in mitochondrial membrane potential. These results revealed the combinatorial regulatory patterns of ATF3 and miR-135a-5p in the regulatory protein interactome, which indicated a clinical significance of the miR-135a-5p-ATF3 protein interaction network in BTZ therapy. This study provides potential evidence for further investigation into BTZ resistance.

Inhibitory role of ATF3 in gastric cancer progression through regulating cell EMT and stemness.

BACKGROUND: Gastric cancer (GC) is one of the most common cancers and the third leading cause of cancer related mortality worldwide. The 5-year survival rate is rather low owing to advanced unresectable and distant metastasis. The EMT has been widely implicated in the stemness, metastatic dormancy, and chemoresistance of different solid tumors. Given the fact that activating transcription factor-3 (ATF3) is a member of the ATF/CREB family of transcription factors and its role in regulation of GC recurrence and metastasis remain poorly understood, the aim of the present study was to investigate its potential impact in epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties and GC aggression. METHODS: To elucidate the potential role of ATF3 in gastric cancer, we utilized SGC-7901 and MGC-803 gastric cancer cell lines as research models and constructed stable cell lines overexpressing ATF3. We conducted a series of assays including cell proliferation, colony formation, cell migration, tumorsphere formation, and invasion to investigate the functional roles of ATF3 in stemness of gastric cancer. The possible effect of ATF3 on epithelial-mesenchymal transition (EMT) was assessed through flow cytometry and qRT-PCR. In vivo functional effect of upregulation of ATF3 on tumor growth was examined in a mouse xenograft model. RESULTS: We found that overexpression of ATF3 inhibited cell proliferation, colony formation, cell migration and invasion. In addition, up-regulation of ATF3 attenuated tumorsphere formation, cell stemness, and potentially decreased expression of EMT markers. Moreover, ATF3 overexpression inhibited tumorigenesis in mouse xenograft model. CONCLUSION: Our data suggest a suppressive role of ATF3 in gastric cancer development. Our findings will provide a potential therapeutic strategy and novel drug target for gastric cancer.

Complete genome sequence of begonia flower breaking virus, a novel member of the genus Potyvirus.

We determined the complete genomic sequence of begonia flower breaking virus (BFBV), a novel putative member of the genus Potyvirus isolated from Begonia bowerae cv. 'Tiger' plants grown in Kunming. The genomic RNA comprises 9540 nucleotides (nt), excluding the 3'-terminal poly(A) tail, and contains a typical open reading frame (ORF) of potyviruses. The ORF consists of 9219 nucleotides and encodes a 3073-amino-acid polyprotein that is predicted to be proteolytically cleaved into 10 mature peptides. Sequence comparison indicated that BFBV shares 43.9-55.12% amino acid sequence identity with known potyviruses and that BFBV shares the highest amino acid sequence identity (55.12%) with beet mosaic virus. The results from the complete genomic sequence analysis further suggest that BFBV is a member of a novel species in the genus Potyvirus.

A study on the local corrosion behavior and mechanism of electroless Ni-P coatings under flow by using a wire beam electrode.

The local corrosion behavior and mechanism of Ni-P coatings in a 3.5 wt% sodium chloride solution with different flow speeds (0 m s-1, 0.5 m s-1, 1 m s-1) were investigated through a wire beam electrode (WBE) with morphological, elemental and electrochemical analyses as well as numerical simulations. It was found that the microstructure of the Ni-P coating was in the shape of broccoli and possessed satisfactory compactness and uniformity. The numerical simulations showed that the speed increased and the static pressure decreased at the local area. Combined with WBE, it was found that the average corrosion potential decreased at that area. The results indicated that the corrosion tendency and corrosion rate of the Ni-P coating were larger at higher speeds, and the corrosion resistance could be improved by the electroless Ni-P coating. WBE was helpful in revealing the local electrochemical information of the Ni-P coating.