ABSTRACT
AIM: To characterize the prevalence of hepatitis C virus (HCV) infection among Chinese intravenous drug users (IDUs). METHODS: A total of 432 adult IDUs (95 women and 337 men) in Shanghai were included in the study. The third-generation Elecsys Anti-HCV assay (Roche Diagnostics GmbH, Sandhofer Strasse 116, D-68305, Mannheim, Germany) was used to screen for antibodies against HCV. The RIBA strip, a supplemental anti-HCV test with high specificity, was performed on all of the samples that tested positive during the initial screening. All of the anti-HCV positive samples were analyzed with a Cobas TaqMan 48 Analyzer (Roche Diagnostics) for direct detection of HCV RNA. All of the HCV RNA-positive samples were sequenced for genotype determination. RESULTS: The preliminary screening identified 262 (60.6%) subjects who were seropositive for HCV. Of the 62 females and 200 males seropositive subjects, 16 (16.7%) and 65 (19.3%), respectively, were confirmed by RIBA, yielding an overall HCV seropositive rate of 18.8%. Four female (6.5%) and 14 male (7.0%) subjects tested positive for HCV RNA, indicating an active infection rate of 4.2% for the entire study population. The 18 HCV RNA-positive serum samples were genotyped. Seven individuals were genotype 1b, and four were genotype 1a. One individual each was infected with genotypes 2a, 2b and 3a. Four subjects were co-infected with multiple strains: two with genotypes 1a and 2a, and two with genotypes 1b and 2a. The active infection rate among HCV-seropositive individuals was 22.2%, which was significantly lower than most estimates. CONCLUSION: The prevalence of HCV is relatively low among IDUs in Shanghai, with a spontaneous recovery rate much higher than previous estimates.
Subject(s)
Drug Users/statistics & numerical data , Hepatitis C/epidemiology , Heroin Dependence/epidemiology , Urban Health/statistics & numerical data , Adult , Biomarkers/blood , China/epidemiology , Female , Genotype , Hepacivirus/genetics , Hepacivirus/immunology , Hepatitis C/blood , Hepatitis C/diagnosis , Hepatitis C Antibodies/blood , Humans , Male , Middle Aged , Prevalence , RNA, Viral/blood , Seroepidemiologic StudiesABSTRACT
448 public health practitioners in the district were selected randomly from Dec. 2010 to Mar. 2011. Blood specimens were collected and tested for anti-T. gondii IgG by ELISA. The result showed that the positive rate was 10.3% (46/448). No significant difference was found between males and females, so as different cities of origin (P > 0.05). The positive rate was higher in > or = 30 age group (14.9%, 29/195) than that in < 30 age group (6.7%, 17/253)(P < 0.05), and the highest sero-prevalence was recorded in 30-39 age group (15.8%, 16/101). The positive rate was higher in subjects engaged in the food production and processing enterprises (12.6%, 36/286) than those in other industries (6.2%, 10/162) (P < 0.05).
