1.
Emerg Infect Dis
; 24(5): 924-925, 2018 05.
Article
in English
| MEDLINE
| ID: mdl-29664374
ABSTRACT
We used real-time PCR to detect Bartonella henselae DNA in 7.9% (5/63) of blood specimens from seronegative patients in Japan suspected of having cat-scratch disease. The combined use of serologic tests and real-time PCR to analyze blood specimens is recommended for the prompt, noninvasive laboratory diagnosis of cat-scratch disease.
Subject(s)
Antibodies, Bacterial/blood , Bartonella henselae/isolation & purification , Cat-Scratch Disease/diagnosis , Cat-Scratch Disease/microbiology , DNA, Bacterial/isolation & purification , Adolescent , Adult , Bartonella henselae/genetics , Child , Child, Preschool , Female , Humans , Japan/epidemiology , Male , Middle Aged , Serologic Tests
2.
Diagn Microbiol Infect Dis
; 87(1): 22-24, 2017 Jan.
Article
in English
| MEDLINE
| ID: mdl-28029424
ABSTRACT
Bartonella henselae strains genetically differ among nations. The utility of Japanese-specific YH-01 strain was investigated in developing indirect fluorescence antibody assay (IFA) for IgM in comparison with conventional IFA employing Houston-1 strain by testing 100 Japanese patients suspected of cat scratch disease. The country-specific IFA greatly improved the accuracy of diagnosis.
