ABSTRACT
BACKGROUND: The goal of this present study was to evaluate the behavior of neonatal rat calvarial osteoblast-like cells cultured on different implant surfaces. MATERIALS: Sandblasted acid-etched (SLA) surfaces of 2 different companies with different alloy properties were used. These were named as SLA-1 and SLA-2. The osteoblasts behavior were analyzed on sand blasted-acid etched (SLA-1) surface (Straumann, Basel, Switzerland), sand blasted-acid etched (SLA-2) surface (Alpha bio, Petach-tikva, Israel), acid-etched surface (Alpha bio), machined surface (Alpha bio). To analyze the effect of titanium surfaces on cell proliferation, cell numbers, and cell viability cells were cultured on titanium discs for 7 days and measurements were held out at 24 hours and on day 7. Cell proliferation rate was assessed by bromodeoxyuridine (BrdU) immunohistochemical technique. Cell morphologies were evaluated by scanning electron microscopy. RESULTS: The highest number of BrdU labeled cells were seen on SLA-1 group at the end of 24 hours. The number of cells was found to be the highest in the acid-etched group on the 7th day, even though there were no significant differences between the groups at the end of 24 hours. Scanning electron microscopy views showed the morphological differences between the groups. Osteoblasts were able to proliferate on all of the tested surfaces, with differences in cell count and DNA synthesis values between the groups. CONCLUSION: Implant surface characteristics may modulate the biological response of osteoblast-like cells depending on the manufacturing techniques and cell culturing procedures.
Subject(s)
Dental Materials/chemistry , Osteoblasts/cytology , Titanium/chemistry , Acid Etching, Dental/methods , Alkaline Phosphatase/analysis , Animals , Animals, Newborn , Antimetabolites , Bromodeoxyuridine , Cell Count , Cell Culture Techniques , Cell Proliferation , Cell Survival , Cells, Cultured , DNA/analysis , Dental Etching/methods , Immunohistochemistry , Microscopy, Electron, Scanning , Osteocalcin/analysis , Rats , Rats, Sprague-Dawley , Skull/cytology , Surface Properties , Time FactorsABSTRACT
OBJECTIVE: The aim of this study was to evaluate the biocompatibility of two different barrier materials in cultures of human osteoblast-like cells (CRL 11372) in vitro. MATERIAL AND METHODS: Polylactic acid (Epi-Guide; EG) and collagen membrane (Bio-Collagen, BC) were examined. To analyze the effect of materials on cell proliferation, cell numbers and cell viability, cells were cultured on the barrier membranes for 24 and 72 h. Cells plated on culture dishes (CD) served as positive controls. Cell proliferation rate was assessed by the bromodeoxyuridine (BrdU) immunohistochemical technique. The cell numbers of each well were counted. Cell viability was estimated by counting the number of cells, which excluded trypan blue solution. Scanning electron microscopy (SEM) was used to observe the interactions between osteoblastic cells and barrier membranes. RESULTS: The highest number of BrdU-labeled cells were seen on CD after both of the time periods. In comparison with the other two groups, BC showed significantly fewer cells after both time periods. Regarding cell numbers, after 24 and 72 h of incubations CD showed the highest number of cells. The number of viable cells was similar for all the groups. After 72 h for the EG group, SEM view showed flat cells. After 72-h time periods, the BC group revealed a weak adhesion of cells to the barriers. CONCLUSION: The results demonstrate that cells were able to proliferate on these materials and EG promoted the proliferation of human osteoblast like cells. We prefer to use the EG membrane.
Subject(s)
Biocompatible Materials/pharmacology , Membranes, Artificial , Osteoblasts/drug effects , Antimetabolites , Bromodeoxyuridine , Cell Adhesion/drug effects , Cell Count , Cell Line , Cell Proliferation/drug effects , Cell Shape/drug effects , Cell Survival/drug effects , Cells, Cultured , Collagen/pharmacology , Coloring Agents , Humans , Immunohistochemistry , Lactic Acid/pharmacology , Microscopy, Electron, Scanning , Polyesters , Polymers/pharmacology , Surface Properties , Time Factors , Trypan BlueABSTRACT
The surgical treatment of a case of anesthesia that occurred with the extrusion of Endomethasone root canal sealer into the mandibular canal is presented. Endomethasone is a neurotoxic root canal sealer containing paraformaldehyde and eugenol. The literature indicates immediate surgical decompression on the extrusion of Endomethasone into the mandibular canal. In our case, the decompression surgery was done 3 weeks after the endodontic mishap. The nearly complete resolution of anesthesia 4 months following the decompression surgery suggests that the neurotoxic effects of Endomethasone are still reversible after 3 weeks.
