Impacts of hydraulic conditions on microplastics biofilm development, shear stresses distribution, and microbial community structures in drinking water distribution pipes.

Both microplastic and biofilm are contamination sources in drinking water, but their integrated impacts on water quality have been rarely studied, especially in drinking water distribution pipes with complex hydraulic conditions. This study explored the impacts of hydraulic conditions (0-2 m/s) on microplastic biofilm (MP-BM) development, shear stresses distribution, and microbial community structures. The research was conducted for two weeks using a pilot test device to simulate practical water pipes. The following were the primary conclusions: (1) According to morphology analysis, clusters (>5 µm) significantly increased in the plastisphere when the flow velocity ranged from 0.55 m/s to 0.95 m/s, and average size of clusters decreased when the flow velocity ranged from 1.14 m/s to 1.40 m/s (2) Characteristics of MP-BM impact shear stress on both plastisphere and pipe wall biofilm. Shear stresses were positively correlated with flow velocity, number of MP-BM, and size of MP-BM, while negatively correlated with diameters of pipes. (3) 31 genera changed strictly and monotonously with the fluid velocity, accounting for 15.42%. Opportunistic pathogens in MP-BM such as Sediminibacterium, Curvibacter, and Flavobacterium were more sensitive to hydraulic conditions. Moreover, microplastics (<100 µm) deserve more attention to avoid human ingestion and to prevent mechanical damage and bio-chemical risks.

Interactive impacts of microplastics and chlorine on biological stability and microbial community formation in stagnant water.

Possibility of human exposure to microplastics (MPs) in water environment has been escalating, and subsequent challenges of MPs to biostability and biosafety in drinking water deserve more attention, especially in stagnant water. The present study explored the integrated impacts of MPs and chlorine on disinfection kinetics, microbial growth, and microbial community formation in drinking water, by setting MPs or microplastic-biofilm (MP-BM) under different disinfection conditions. The following were the primary conclusions: (1) The presence of MP and MP-BM led to the deterioration of water indices (especially turbidity) when chlorine was less than 1 mg/L. (2) MP/MP-BM accelerated the decay of disinfectants and MP-BM consumed more rapidly. Meanwhile, chlorine contributed to the level of BRP, ranging from 4.78 × 105 CFU/mL to 1.42 × 107 CFU/mL. (3) MP/MP-BM and chlorine integrally shaped microbial communities in water samples and biofilm samples. Microbial dissimilarity between isolated and hybrid MP-BM indicated manners of microbial field or non-contact communication. Microbial abundance and OPs were effectively controlled when chlorine was over 1 mg/L. (4) According to time-lag differential equations simulation, impulsive chlorination contributed to controlling microbial risks and DBPs induced by MP/MP-BM and water stagnation.