[Optimization of water extraction condition of LiYan granule by Doehlert design].

OBJECTIVE: To study optimal water extraction condition of LiYan granule. METHODS: HPLC methods were developed for the determination of irisfloremin in LiYan granule. The contents of irisfloremin and extraction rate were selected as assessment indices. Three factors were studied by Doehlert design, including water-adding amount, decoction time and times of decoction. RESULTS: The optimal extracting condition was water consumed as 8 times of curd herb, and 3 times for 2 hours each time. CONCLUSION: The water extraction process is feasible, and the experiment result can provide basic and instruction data for the ascertainment of water extraction process of LiYan granule.