Heterologous Expression of a Ferritin Homologue Gene PpFer1 from Prunus persica Enhances Plant Tolerance to Iron Toxicity and H2O2 Stress in Arabidopsis thaliana.

In plants, ferritin proteins play an important role in iron (Fe) storage which contributes to plant growth and development. However, the biological functions of ferritins in fruit trees are essentially unknown. In this study, three Ferritin genes were isolated from 'Zhentong No. 3' peach, which were named PpFer1-PpFer3. The expression levels of these genes were different in distinct tissues/organs. Notably, PpFer1 was the most abundantly expressed Ferritin family gene in all tested tissues of 'Zhentong No. 3' peach; its expression levels were significantly enhanced throughout the entire peach seedling under Fe toxicity and H2O2 stress, particularly in the leaves. In addition, over-expression of PpFer1 was effective in rescuing the retarded growth of Arabidopsis fer1-2 knockout mutant, embodied in enhanced fresh weight, primary root length, lateral root numbers, total root length, total leaf chlorophyll, stomatal conductance (Gs), net photosynthetic rate (Pn), transpiration rate, and tissue Fe concentration. This study provides insights into understanding the molecular mechanisms of Fe storage and sequestration in perennial fruit trees.

Single ultrasound-guided thoracic paravertebral block with a large volume of anesthetic for microwave ablation of lung tumors.

Objective: To compare single ultrasound-guided thoracic paravertebral block (TPVB) using a large volume of anesthetic with local anesthesia (LA) in computed tomography (CT)-guided pulmonary microwave ablation. Subjects and methods: Eighty patients who underwent CT-guided microwave ablation of pulmonary tumors were randomly divided into the TPVB group and the LA group. Patients of the TPVB group were anesthetized with a single injection of a large volume (40 ml) of 0.375% ropivacaine injection at T4, and those of the LA group had local infiltration by the surgeon at the puncture site, and emergency rescue with propofol injection was administered when the patient could not tolerate pain in either group. The following variables were recorded in both groups: general conditions; volume of propofol injection for emergency rescue during ablation; visual analog scale (VAS) scores during ablation and at 0, 2, 12, and 24 h after ablation; the need to use analgesics for rescue within 24 h after ablation; number of ablations; number of punctures performed by the surgeon; patient's movements during puncturing; and puncturing-associated complications. Results: Compared with the TPVB group, the amount of emergency use of propofol injection was significantly more in the LA group (P < 0.05). There were no significant differences in the VAS scores recorded intraoperatively and at 0, 2, 12, and 24 h after ablation between the two groups (P > 0.05). There was a significant difference in the patient's movements upon puncturing between the two groups (P < 0.05), but there were no significant differences in the numbers of punctures and ablations between the two groups (P > 0.05). The number of patients using analgesics within 24 h after the operation was also more in the LA group than in the TPVB group, and the difference between the two groups was statistically significant (P < 0.05). Conclusion: Single ultrasound-guided TPVB with a large volume of anesthetic offers effective analgesia for microwave ablation of lung tumors, helping the patient cooperate with the operating surgeon to reduce injury from multiple lung punctures. Further studies are recommended to validate these findings.

H2FPEF score predicts 1-year rehospitalisation of patients with heart failure with preserved ejection fraction.

BACKGROUND: Heart failure with preserved ejection fraction (HFpEF) has received widespread attention in recent years. There is currently a lack of valuable predictors for the prognosis of this disease. Here, we aimed to identify a non-invasive scoring system that can effectively predict 1-year rehospitalisation for patients with HFpEF. METHODS: We included 151 consecutive patients with HFpEF in a prospective cohort study and investigated the association between H2FPEF score and 1-year readmission for heart failure using multivariate Cox regression analysis. RESULTS: Our findings indicated that obesity, age >70 years, treatment with ≥2 antihypertensives, echocardiographic E/e' ratio >9 and pulmonary artery pressure >35 mm Hg were independent predictors of 1-year readmission. Three models (support vector machine, decision tree in R and Cox regression analysis) proved that H2FPEF score could effectively predict 1-year readmission for patients with HFpEF (area under the curve, 0.910, 0.899 and 0.771, respectively; p<0.001). CONCLUSION: Our study demonstrates that the H2FPEF score has excellent predictive value for 1-year rehospitalisation of patients with HFpEF.

Serum Chemerin as a Novel Prognostic Indicator in Chronic Heart Failure.

Background It has been documented that circulating chemerin is associated with inflammation, metabolic syndrome, and coronary artery disease. The present study was aimed to evaluate the prognostic value of serum chemerin in patients with chronic heart failure. Methods and Results We included 834 patients with chronic heart failure in a prospective cohort study and investigated the association between serum chemerin and clinical outcomes using multivariate Cox regression analysis. Patients with higher chemerin levels tended to be older and women and were more likely to experience hypertension, diabetes mellitus, and hyperlipemia. Cox regression analysis showed that chemerin was a significant predictor of major adverse cardiac events (hazard ratio, 1.83; 95% CI, 1.31-2.96) after adjustment for conventional risk factors. Net reclassification and integrated discrimination improvements for major adverse cardiac events were markedly improved by addition of chemerin to the reference model. In addition, chemerin was an independent predictor of all-cause mortality (hazard ratio, 1.67; 95% CI, 1.21-2.73) after multivariable adjustment. Furthermore, the Kaplan-Meier survival analysis revealed that chemerin was a prognostic indicator of major adverse cardiac events in patients with chronic heart failure and NT-proBNP (N-terminal pro-B-type natriuretic peptide) levels above and below the median. Conclusions Our study suggests that chemerin is a novel serum marker for predicting major adverse cardiac events in patients with chronic heart failure.

Diagnostic and prognostic value of biomarkers in acute myocardial infarction.

The incidence of acute myocardial infarction (AMI) has been increasing rapidly in recent years, seriously endangering human health. Cardiac biomarkers play critical roles in the diagnosis and prognosis of AMI. Troponin is a highly sensitive and specific biomarker for AMI diagnosis and can independently predict adverse cardiac events. Other biomarkers such as N-terminal B-type natriuretic peptide and C reactive protein are also valuable predictors of cardiovascular prognosis. Recently, several novel biomarkers have been identified for the diagnosis and risk assessment in patients with AMI. A multibiomarker approach can potentially enhance the diagnostic accuracy and provide more information for the early risk stratification of AMI. In this review, we will summarise the biomarkers discovered in recent years and focus on their diagnostic and prognostic value for patients with AMI.

Serum Vaspin as a Predictor of Adverse Cardiac Events in Acute Myocardial Infarction.

Background The involvement of vaspin (visceral adipose tissue-derived serpin) in the development of atherosclerotic cardiovascular diseases has been documented. This study was designed to explore the prognostic value of serum vaspin in patients with acute myocardial infarction ( AMI ). Methods and Results We included 1036 AMI patients in a cohort study and determined the association between serum vaspin and major adverse cardiac events ( MACE ) using Cox regression analysis. The receiver operating characteristic curve indicated that serum vaspin could significantly differentiate patients with MACE , and the optimal cutoff value was 0.62 ng/mL. The Kaplan-Meier survival curve showed that patients with lower vaspin levels had higher incidence of MACE . Multivariate Cox regression analysis revealed that low vaspin was an independent predictor of MACE (hazard ratio: 0.74; 95% CI , 0.48-0.96; P=0.029), together with age; previous histories of AMI , heart failure, hypertension, and diabetes mellitus; Killip class; revascularization; CRP (C-reactive protein); and NT-proBNP (N-terminal pro-B-type natriuretic peptide). Integrated discrimination and net reclassification improvements for MACE were significantly improved by addition of vaspin to the model of traditional risk factors. Moreover, low vaspin was a valuable predictor of heart failure hospitalization (hazard ratio: 0.58; 95% CI , 0.37-0.89; P=0.005) and recurrent AMI (hazard ratio: 0.72; 95% CI , 0.53-0.95; P=0.036) after adjustment for conventional cardiovascular risk factors. Conclusions Our study suggests that serum vaspin is a significant prognostic marker of MACE in AMI patients.

Bias-scanning based tunable LSPR sensor.

The principle and the characteristics of the bias-scanning based tunable localized surface plasmon resonance (LSPR) sensors for environmental refractive index have been theoretically and numerically investigated in detail. The sensors exhibit linear negative-shifts in the scattering-bias spectral position when the refractive index of the surrounding medium increases. By bias-scanning, a single-wavelength measurement for sensing the environmental refractive index can be realized effectively. In addition, we demonstrate that the sensing performance of the designed sensor can be adjusted by nano-scale manipulations of metal nanoparticles' sizes and shapes. The proposed devices may pave the way for the development of electrochemical sensors that can convert spectrum scanning into bias scanning.

The Influence of Fiber Cross-Section on Fabric Far-Infrared Properties.

Far-infrared radiation (FIR) possesses various promising properties that are beneficial to an individuals' health. Exploring the interaction between fiber shapes and FIR performance is thought to be a significant means to develop highly-efficient FIR textile products. In this study, a non-additive triangular polyamide (PA) fiber showed excellent FIR properties in both theoretical simulation and experimental verification aspects. The triangular PA fiber affords a higher probability to facilitate large optical path difference, improving both FIR absorption and emission. Textiles woven with the specific triangular PA fiber achieved a remarkable emissivity of 91.85% and temperature difference of 2.11 Celsius, which is obviously superior to the reference circular fiber (86.72%, 1.52 Celsius). Considering the low cost, environmental stability, facile fabrication, as well as being environmentally friendly, this non-additive triangular PA fiber has great potential for high-performance and cost-effective FIR textiles in the future.

Serum corin is associated with the risk of chronic heart failure.

It has been well documented that corin is a critical protease involved in the regulation of blood pressure and cardiac function. We performed a case-control study to determine whether serum corin is associated with the risk of chronic heart failure (CHF). We included 484 consecutive CHF patients and 484 control subjects to investigate the potential relationship between serum corin and CHF using logistic regression analysis. Compared with healthy controls, the CHF patients were more likely to have histories of hypertension and diabetes, and had higher levels of N-terminal pro-brain natriuretic peptide and lower levels of corin. The odds ratios of ischemic and non-ischemic HF were significantly reduced with the growing levels of serum corin after multivariate adjustment. Moreover, the decrease in serum corin levels seemed to be associated with the severity of CHF. In conclusion, our study suggested that serum corin levels were reduced in CHF patients and inversely correlated with the incidence of ischemic and non-ischemic HF.

Hierarchically Three-Dimensional Nanofiber Based Textile with High Conductivity and Biocompatibility As a Microbial Fuel Cell Anode.

Microbial fuel cells (MFCs) encompass complex bioelectrocatalytic reactions that converting chemical energy of organic compounds to electrical energy. Improving the anode configuration is thought to be a critical step for enhancing MFCs performance. In present study, a hierarchically structured textile polypyrrole/poly(vinyl alcohol-co-polyethylene) nanofibers/poly(ethylene terephthalate) (referred to PPy/NFs/PET) is shown to be excellent anode for MFCs. This hierarchical PPy/NFs/PET anode affords an open porous and three-dimensional interconnecting conductive scaffold with larger surface roughness, facilitating microbial colonization and electron transfer from exoelectrogens to the anode. The mediator-less MFC equipped with PPy/NFs/PET anode achieves a remarkable maximum power density of 2420 mW m(-2) with Escherichia coli as the microbial catalyst at the current density of 5500 mA m(-2), which is approximately 17 times higher compared to a reference anode PPy/PET (144 mW m(-2)). Considering the low cost, low weight, facile fabrication, and good winding, this PPy/NFs/PET textile anode promises a great potential for high-performance and cost-effective MFCs in a large scale.

Advanced light-trapping effect of thin-film solar cell with dual photonic crystals.

A thin-film solar cell with dual photonic crystals has been proposed, which shows an advanced light-trapping effect and superior performance in ultimate conversion efficiency (UCE). The shapes of nanocones have been optimized and discussed in detail by self-definition. The optimized shape of nanocone arrays (NCs) is a parabolic shape with a nearly linearly graded refractive index (GRI) profile from the air to Si, and the corresponding UCE is 30.3% for the NCs with a period of 300 nm and a thickness of only 2 µm. The top NCs and bottom NCs of the thin film have been simulated respectively to investigate their optimized shapes, and their separate contributions to the light harvest have also been discussed fully. The height of the top NCs and bottom NCs will also influence the performances of the thin-film solar cell greatly, and the result indicates that the unconformal NCs have better light-trapping ability with an optimal UCE of 32.3% than the conformal NCs with an optimal UCE of 30.3%.