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1.
Food Chem ; 456: 139946, 2024 Jun 02.
Article in English | MEDLINE | ID: mdl-38852450

ABSTRACT

To effectively monitor multi-residues of penicillin antibiotics (PENs) in milk, we developed a novel ratiometric electrochemical aptasensor enabling simultaneous detection of PENs. The aptasensor employed a broad-spectrum aptamer as a recognition element, niobium carbide functionalized with methylene blue (Nb2C-MB) as a reference signal generator, and a ferrocene-labeled aptamer (Fc-Apt) as an output signal. Electrodes were modified with Fe-N-C doped carbon nanotubes (Fe-N-C-CNTs) to amplify detection signals further. During detection, Fc-Apt binding to PENs decreased Fc current intensity (IFc) and increased MB current intensity (IMB). The simultaneous detection of PENs was achieved using IMB/IFc as a quantitative signal. Under optimal conditions, a good linear relationship between IMB/IFc and antibiotic concentration was observed, indicating the aptasensor had a robustness. The limits of detection of aptasensor for four penicillin antibiotics and their mixed targets were 0.093-0.191 nM. This work provides a new approach to multi-residue detection of the same class of antibiotics.

2.
Talanta ; 269: 125508, 2024 Mar 01.
Article in English | MEDLINE | ID: mdl-38070284

ABSTRACT

Penicillin antibiotics (PENs) play an important role in killing pathogenic bacteria. However, the residues of various penicillin antibiotics in milk gradually accumulate in the human body with the increase of milk intake, which causes direct harm to the human body. Aptamers can be used as recognition element of sensors. It is great significance to use broad-spectrum aptamers for simultaneous detection of PENs. In this study, we reported the screening and identification of DNA aptamers for PENs. The aptamers were screened by graphene oxide-systematic evolution of ligands by exponential enrichment (GO-SELEX). The broad-spectrum aptamers with high affinity and specificity were successfully obtained after 13 rounds of screening. The affinity and specificity of candidate aptamers were analyzed by a GO fluorescence competition method. Further sequence analysis revealed that a truncated 47 nt aptamer (P-11-1) had a higher affinity than the original 79 nt aptamer. The truncated aptamer P-11-1 was used as a recognition element, and an electrochemical aptasensor was prepared using gold nanoparticles (AuNPs) combined with ferroferric oxide-multi walled carbon nanotube (Fe3O4-MWCNTs) complex. The results showed that the developed aptasensor achieved the simultaneous detection of PENs in milk samples across a concentration range of 2 nM-10,000 nM, achieving a limit of detection of 0.667 nM. This methodology provided a simple and sensitive new thinking for antibiotic multi-residue detection.


Subject(s)
Aptamers, Nucleotide , Biosensing Techniques , Metal Nanoparticles , Humans , Animals , Milk/chemistry , Penicillins/analysis , Gold/chemistry , Metal Nanoparticles/chemistry , Aptamers, Nucleotide/chemistry , SELEX Aptamer Technique/methods , Biosensing Techniques/methods , Limit of Detection
3.
Medicine (Baltimore) ; 102(13): e33433, 2023 Mar 31.
Article in English | MEDLINE | ID: mdl-37000063

ABSTRACT

This research aimed to combine serum NLR-pyrin domain containing 1 (NLRP1) levels and collateral circulation to assess ischemic stroke patients and predict the prognoses of the patients. This present prospective observational study enrolled 196 ischemic stroke patients. All patients underwent CTA as well as digital subtraction angiography (DSA) to assess collateral circulation by American Society of Interventional and Therapeutic Neuroradiology/Society of Interventional Radiology (ASITN/SIR). In addition, we collected serum samples from 100 patients with carotid atherosclerosis as controls. The serum NLRP1, tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-1ß and C-reactive protein (CRP) levels were measured by enzyme-linked immunosorbent assay (ELISA). The age, BMI, sex, smoke condition, diastolic blood pressure, systolic blood pressure, National Institutes of Health Stroke Scores (NIHSS), modified Rankin Scale (mRS) scores, imaging indicators and the levels of triglyceride, total cholesterol (TC), low-density leptin cholesterol (LDLC), high-density leptin cholesterol of all subjects were recorded. All data used SPSS 18.0 to statistical analyses. The serum levels of NLRP1 were remarkably enhanced in the ischemic stroke patients compared with the carotid atherosclerosis patients. The NIHSS score, the mRS score after 90 days and the levels of NLRP1, CRP, TNF-α IL-6 and IL-1ß of ischemic stroke patients in the ASITN/SIR grade 0 to 2 group were remarkably elevated than the ischemic stroke patients in ASITN/SIR grade 3 to 4 group. Spearman analysis supported that a positive correlation existed among the NLRP1, CRP, IL-6, TNF-α, and IL-1ß levels. The NIHSS score, infarct volume and the levels of NLRP1, IL-6, TNF-α, and IL-1ß of ischemic stroke patients in the mRS score ≥ 3 group were remarkably elevated than the ischemic stroke patients in the mRS score ≤ 2 group. ASITN/SIR grade and NLRP1 could be potential diagnostic biomarkers of poor prognosis of ischemic stroke patients. It was found that NLRP1, ASITN/SIR grade, infarct volume, NIHSS, IL-6, and IL-1ß were the risk factors for bad prognosis of ischemic stroke patients. This study showed that the serum NLRP1 levels were remarkably decreased in ischemic stroke patients. In addition, the serum NLRP1 levels and ASITN/SIR grade could predict the prognosis of ischemic stroke patients.


Subject(s)
Brain Ischemia , Carotid Artery Diseases , Ischemic Stroke , Stroke , Humans , Leptin , Collateral Circulation/physiology , Clinical Relevance , Interleukin-6 , Tumor Necrosis Factor-alpha , Computed Tomography Angiography/methods , Infarction , Cholesterol , Brain Ischemia/therapy , NLR Proteins
4.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-1003898

ABSTRACT

ObjectiveTo compare the short-term effectiveness of the three different manipulations for atlantoaxial joint disorders and their effects on surface electromyography of sternocleidomastoid muscle. MethodsNinty patients with atlantoaxial joint disorders were randomly divided into the tendon relaxing manipulation group, the tendon relaxing plus rehabilitation manipulation group, and the conventional manipulation group, with 30 cases in each group, and each group of patients received the corresponding manipulation treatment for 2 weeks. The changes of visual analogue score (VAS) of occipital neck pain, evaluation scale for cervical vertigo (ESCV), and averaged electromyography (AEMG) of surface electromyography of bilateral sternocleidomastoid muscles before and after the treatment were observed, and the clinical effectiveness and safety of the patients were compared among groups. ResultsThe VAS scores of patients in each group decreased, and the ESCV scores increased after treatment (P<0.01), and the tendon relaxing manipulation group and the tendon relaxing plus rehabilitation manipulation group were significantly better than the conventional manipulation group (P<0.01). The AEMG of the bilateral sternocleidomastoid muscles of the three groups increased after treatment (P<0.01); when compared among the three groups, the AEMG of the bilateral sternocleidomastoid muscles of the tendon relaxing plus rehabilitation manipulation group was higher than that of the tendon relaxing manipulation group, and the tendon relaxing manipulation group was higher than that of the conventional manipulation group (P<0.05 or P<0.01). The cure and markedly effective rates of the tendon relaxing manipulation group, the tendon relaxing plus rehabilitation manipulation group, and the conventional manipulation group were 56.67%, 86.67%, and 36.67% respectively, showing statistically difference (K=10.21, P<0.01). ConclusionThe tendon relaxing manipulation and tendon relaxing plus rehabilitation manipulation can effectively improve the symptoms of vertigo, headache, and neck pain for patients with atlantoaxial joint disorders, and can improve the contraction function of sternocleidomastoid muscle, whose effectiveness are better than that of conventional manipulation.

5.
Am J Transl Res ; 14(5): 2768-2778, 2022.
Article in English | MEDLINE | ID: mdl-35702093

ABSTRACT

BACKGROUND: Polycystic ovary syndrome (PCOS) patients are prone to early pregnancy loss. Studies have found that the blood fibrinolytic activity of PCOS patients is closely related to the high abortion rate. At present, it is considered that the combination of traditional Chinese and Western medicine in the treatment of PCOS with threatened abortion has a good effect on fetal protection. Therefore, the clinical efficacy of the combination of traditional Chinese and Western medicine in the treatment of PCOS with threatened abortion was systematically evaluated, and the effect of traditional Chinese medicine on the blood fibrinolytic status of patients was also evaluated. METHODS: The clinical randomized controlled trials (RCTs) of integrated traditional Chinese and Western medicine in the treatment of PCOS with threatened abortion were reviewed from four Chinese databases (CNKI, WANFANG, VIP, and SINOMED) and two English databases (PubMed, EMBASE) from their time of establishment to May 2021. According to the inclusive and exclusive criteria, tests were selected, data were extracted and evaluated, the systematic review was carried out by description analysis, and the meta-analysis was conducted using RevMan 5.3 software from Cochrane Collaboration. Begg's and Egger's tests were performed for assessing symmetries of funnel plot by the software Stata 16.0. RESULTS: 6 RCTs involving 361 participants were included. Meta-analysis results showed that integrated traditional Chinese and Western medicine can increase the total efficacy compared with the control groups [RR=1.22, 95% CI (1.10, 1.35), P=0.0002]. Meanwhile, the improvement of TCM syndrome integral [MD=-2.11, 95% CI (-2.56, -1.66), P<0.00001], D-II [MD=-0.29, 95% CI (-0.49, -0.09), P=0.004], Hcy [MD=-3.02, 95% CI (-3.64, -2.39, P=0.00001], PAI-I [SMD=-2.38, 95% CI (-2.82, -1.73), P<0.00001] were better than a single Western medicine treatment. And it can increase the level of ß-HCG [SMD=3.44, 95% CI (1.87, 5.01), P<0.0001], P [MD=7.70, 95% CI (7.10, 8.31), P<0.00001], E2 [MD=205.36, 95% CI (140.93, 269.80), P<0.00001]. The funnel plot, Egger's test (P=0.210), and Begge's test (P=0.573) suggested that there was no evidence of substantial publication bias. CONCLUSIONS: The effect of integrated traditional Chinese and Western medicine is better than that of Western medicine alone in the treatment of PCOS with threatened abortion, and it has certain advantages in improving the fibrinolytic status of patients. However, due to the limitation of the quality and quantity of the included literature, more rigorous and larger sample studies are needed to confirm these conclusions in the future.

6.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-689990

ABSTRACT

<p><b>OBJECTIVE</b>To explore the safety and effectiveness of percutaneous transforaminal endoscopic BEIS technology for lumbar lateral recess stenosis in the elderly.</p><p><b>METHODS</b>From February 2014 to May 2016, 21 patients with lumbar lateral recess stenosis in elderly were treated with percutaneous endoscopic BEIS. There were 13 males and 8 females, aged from 70 to 85 years old with an average of 74.3 years. Preoperative, 1 and 12 months postoperative visual analogue scale(VAS) scores and Oswestry Disability Index(ODI) were statistically analyzed. MacNab was used to assess the clinical effects.</p><p><b>RESULTS</b>All the operations were successful. The time ranged from 90 to 130 min with an average of 110 min. All the patients were followed up for 12 to 38 months with an average of 18 months. Preoperative, 1 and 12 months postoperative VAS scores were 8.47±1.23, 1.78±0.72, 0.68±0.32, and ODI scores were 32.48±10.03, 19.53±3.55, and 5.15±1.02, respectively. Postoperative scores of VAS and ODI were obviously improved(<0.05). According to modified MacNab standard to evaluate the clinical effects, 14 cases obtained excellent results, 5 good, 2 fair. Lower limb paresthesia occurred in 1 case, and the condition was restored at 3 months postoperatively with conservative treatment. One patient was complicated with emphysema before operation secondary to pulmonary infection, and was effectively controlled with regulate antibiotic therapy. No infection of vertebral body or intervertebral space, no injuries of blood vessels or nerve root, no tear of dura, or the leakage of cerebrospinal fluid were found.</p><p><b>CONCLUSIONS</b>Percutaneous transforaminal endoscopic BEIS is a safe and effective method for lumbar lateral recess stenosis in the elderly.</p>


Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Diskectomy, Percutaneous , Endoscopy , Lumbar Vertebrae , Lumbosacral Region , Pathology , Spinal Stenosis , General Surgery , Treatment Outcome
7.
J Chem Phys ; 137(10): 104307, 2012 Sep 14.
Article in English | MEDLINE | ID: mdl-22979859

ABSTRACT

In this work, we report on the spectroscopy and dynamics of the quasi-linear S(2) state of chlorocarbene, CHCl, and its deuterated isotopologue using optical-optical double resonance (OODR) spectroscopy through selected rovibronic levels of the S(1) state. This study, which represents the first observation of the S(2) state in CHCl, builds upon our recent examination of the corresponding state in CHF, where pronounced mode specificity was observed in the dynamics, with predissociation rates larger for levels containing bending excitation. In the present work, a total of 14 S(2) state vibrational levels with angular momentum l = 1 were observed for CHCl, and 34 levels for CDCl. The range of l in this case was restricted by the pronounced Renner-Teller effect in the low-lying S(1) levels, which severely reduces the fluorescence lifetime for levels with K(a) > 0. Nonetheless, by exploiting different intermediate S(1) levels, we observed progressions involving all three fundamental vibrations. For levels with long predissociation lifetimes, rotational constants were determined by measuring spectra through different intermediate J levels of the S(1) state. Plots of the predissociation linewidth (lifetime) vs. energy for various S(2) levels show an abrupt onset, which lies near the calculated threshold for elimination to form C((3)P) + HCl on the triplet surface. Our experimental results are compared with a series of high level ab initio calculations, which included the use of a dynamically weighted full-valence CASSCF procedure, focusing maximum weight on the state of interest (the singlet and triplet states were computed separately). This was used as the reference for subsequent Davidson-corrected MRCI(+Q) calculations. These calculations reveal the presence of multiple conical intersections in the singlet manifold.


Subject(s)
Methane/analogs & derivatives , Methane/chemistry , Quantum Theory , Spectrophotometry
8.
Zhonghua Wei Chang Wai Ke Za Zhi ; 14(11): 871-4, 2011 Nov.
Article in Chinese | MEDLINE | ID: mdl-22116723

ABSTRACT

OBJECTIVE: To discuss the value of multiple-slice spiral CT diagnosis of atypical intraabdominal hernia. METHODS: The clinical and CT findings of 16 cases of atypical intraabdominal hernia confirmed surgically were retrospectively analysed. RESULTS: In all the 16 cases, the contents of hernia were small bowels and the mesentery. Nine cases were caused by the adhesion after abdominal operations or infection, 6 by the mesenteric foramen, and 1 by the gap of pelvic peritoneum. The main CT findings were as follows:(1)the obstructed small bowels gathered abnormally and showed cluster shape(9 cases); the walls of the bowels thickened with edema and showed "target" sign with exudate in the neighboring spaces(5 cases); other part of the abdominal cavity became empty for lack of small bowels(4 cases).(2) Abnormal arrangement of the branches of mesenteric vessel, which appeared gathered or pulled or rigid and displacement of the main branches to left or right(12 cases). Thickened mesenteric vessel (4 cases): torsion of mesentery with "whirlpool" sign (3 cases). (3) With the help of multiplanar reformation, 5 cases showed the evidence of hernia rings. In the ring area, there were gathered or pulled or rigid and radiating mesenteric vessel and the dilated or effusion bowels in cluster arrangement forming "parachute" and "bundle of balloons" sign. CONCLUSION: CT manifestations of atypical intraabdominal hernia has some specific characteristics, which is of important value for clinical diagnosis and treatment.


Subject(s)
Hernia, Ventral/diagnostic imaging , Tomography, Spiral Computed/methods , Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Male , Middle Aged , Young Adult
9.
J Chem Phys ; 135(10): 104315, 2011 Sep 14.
Article in English | MEDLINE | ID: mdl-21932901

ABSTRACT

In this work, we report on our full results of the spectroscopic analysis of the quasi-linear S(2) state of the prototypical halocarbene, CHF, and its deuterated isotopomer CDF using optical-optical double resonance spectroscopy through the S(1) state. A total of 51 S(2) state vibrational levels with angular momenta in the range [script-l] = 0-3 were observed for CHF, and 76 levels for CDF. Progressions involving all three fundamental vibrations were observed, and rotational constants were determined for each of these levels by measuring spectra through different intermediate J levels of the S(1) state. Our experimental results are in excellent agreement with the predictions of vibrational calculations using the discrete variable representation method. The variational vibrational calculations were performed with an analytic potential energy surface fit to ab initio data by the method of interpolating moving least squares. The ab initio data are Davidson-corrected multi-reference configuration interaction calculations based on a state-averaged multiconfigurational self-consistent field reference incorporating a generalized dynamic weighting scheme.

10.
J Chem Phys ; 135(10): 104316, 2011 Sep 14.
Article in English | MEDLINE | ID: mdl-21932902

ABSTRACT

In this work, we report on our full results of the dynamics of the quasi-linear, predissociated S(2) state of the prototypical halocarbene, CHF, and its deuterated isotopomer CDF using optical-optical double resonance spectroscopy through the S(1) state. Homogeneous linewidths were determined for a total of 51 S(2) state vibrational levels with angular momenta in the range [script-l] = 0-3 for CHF, and 76 levels for CDF. Progressions involving all three fundamental vibrations were observed. The linewidth data reveal pronounced mode specificity for both CHF and CDF, where pure bending states have the largest linewidths. For CDF, the linewidths are uniformly narrower. Calculated (CCSD(T)/aug-cc-pVQZ//MP2/aug-cc-pVQZ) stationary points on the CHF potential energy surface show that two dissociative pathways are available at the energies accessed in this experiment: dissociation on the triplet surface, over a barrier, to form C((3)P) + HF, and dissociation to ground state CF + H products. The former is excluded as a primary channel based on the small spin-orbit coupling in this system. A 27-state dynamically weighted full-valence complete active space self-consistent field calculation was performed with maximal weight focused on the S(2) state, which was then used as a reference for Davidson-corrected multireference configuration interaction calculations MRCI(+Q) of the three lowest A(') and two lowest A(") states. These calculations reveal the presence of multiple conical intersections in the singlet manifold. Consistent with our experimental results, the most important of these involves the repulsive S(3) state, which conically intersects with S(2) at linearity.

11.
Langenbecks Arch Surg ; 396(1): 53-62, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20582601

ABSTRACT

PURPOSE: The aim of this study was to evaluate the benefits and harms of primary closure versus T-tube drainage after common bile duct (CBD) exploration for choledocholithiasis. METHODS: A literature search of MEDLINE (PubMed), EMBASE, and the Cochrane Library was done to identify randomized controlled trials assessing the benefits and harms of primary closure versus T-tube drainage after CBD exploration from Jan. 1990 to Apr. 2010. A meta-analysis was set up to distinguish overall difference between the primary closure and the T-tube drainage group. RESULTS: There were statistically significant differences between groups: biliary complications (odds ratio (OR) 95% confidence interval (CI), 0.42 (0.19-0.92); P = 0.03), main complications (OR 95% CI, 0.46 (0.23-0.90); P = 0.02), operating time (weighted mean difference (WMD) 95% CI, -19.53 (-29.35 to -9.71); P < 0.0001), and hospital stay (WMD 95% CI, -4.16 (-7.07 to -1.24); P = 0.005) except peri-operative mortality (OR 95% CI, 0.83 (0.11-6.37); P = 0.86), residual stones (OR 95% CI, 0.70 (0.22-2.25); P = 0.55), and abdominal collections (OR 95% CI, 1.93 (0.34-10.76); P = 0.46). And the result of wound infection (OR 95% CI, 0.38 (0.14-1.02); P = 0.05) tended to favor the primary closure group. CONCLUSION: The primary closure might be as effective as T-tube drainage after choledochotomy in the prevention of the development of post-operative complications.


Subject(s)
Abdominal Wound Closure Techniques , Choledochostomy/methods , Drainage/methods , Gallstones/surgery , Postoperative Complications/etiology , Gallstones/mortality , Hospital Mortality , Humans , Length of Stay/statistics & numerical data , Postoperative Complications/mortality , Randomized Controlled Trials as Topic , Survival Analysis , Suture Techniques
12.
Chinese Journal of Orthopaedics ; (12): 582-586, 2011.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-416669

ABSTRACT

Objective To investigate the methods and therapeutic effects of image-guided percutaneous needle iniection of methylprednisolone and injectable calcium sulfate for simple bone cysts.Methods Thirty-seven patients with simple bone cysts from 0ctober 2006 to August 2010 were analysed retrospectively in our hospital,including 26 males and 11 females with the average age of 13.2 years(range,8-22 years).Five cases of proximal femus lesions,with proximal thigh pain,limp and other symptoms.Thirty-two cases of proximal humeral lesions,16 patients had proximal pain and other symptoms of upper arm,the other 16 cases were asymptomatic.Preoperative AP and lateral X-ray.CT and MRl were taken.Under the C-arm X-ray monitor.two needles were inserted into the proximal and distal of cysts respectively,Omnipaque contrast was iniected to confirm the two needles is interlinked,then repeated rinsing with normal saline,then 120 mg methylprednisolone and iniectable calcium sulfate were injected,till the cysts were full up.Patients after treatment were assessed according to modified Neer X-ray criteria.Resuits The average hospitalization was 2.3 days (range.1-3 days).X-ray was reviewed every month,additional injection was performed if bone cysts stopped decreasing for 2 consecutive months,iniection 2 times in 6 eases,and 31 cases were injected only once.After 3 months follow.up 37 cases,according to modified Neer X-ray criteria,6 eases regarded as grade Ⅱ,8 as grade Ⅲ,23 as grade Ⅳ;after 6 months,31 patients were followed up,including 2 cases as grade Ⅱ,4 cases as grade Ⅲ,25 as grade Ⅳ;after 24 months of follow-up 26 cases,3 as grade Ⅲ,23 as grade Ⅳ;after 36 months follow-up,19 cases were all grade Ⅳ.Conclusion Imaging-guided percutaneous iniection of methylprednisolone and inieetable calcium sulfate for simple bone cysts has demonstrated,with less trauma,lower complications incidence and quicker recovery.

13.
Hepatogastroenterology ; 57(104): 1360-2, 2010.
Article in English | MEDLINE | ID: mdl-21443086

ABSTRACT

BACKGROUND: This study aims to explore the relationship between spleen arterial blood flow (SBF) with platelet count, spleen index (SPI) and the serum nitric oxide (NO) level of patients with liver cirrhosis and to investigate the role of SBF in the development of hypersplenism. METHODOLOGY: Platelet count, SPI, SBF and serum NO levels were evaluated in 100 patients with liver cirrhosis caused by hepatitis B with hypersplenism (cirrhosis group) and 30 healthy persons without hypersplenism (control group). RESULTS: Platelet count in cirrhosis group and control group was 57.0 +/- 25.6 x 109/L and 205.8 +/- 47.4 x 109/L (p = 0.000), SBF was 535.7 +/- 263.7 milmin and 172.2 +/- 66.9 ml/min (p = 0.000), and serum NO level was 98.51 +/- 23.06 micromol/L and 48.43 +/- 19.47 micromol/L (p = 0.000). Linear correlations were made between SBF and platelet count in cirrhosis group (r = -0.573, p = 0.000), SBF and SPI (r = 0.607, p = 0.01), SBF and serum NO level (r = 0.754, p = 0.000). Moreover, serum NO level increased as liver disease aggravated (82.50 +/- 15.04 pmol/L in Child grade A, 94.61 +/- 21.00 micromol/L in grade B and 116.83 +/- 18.03 micromol/L in grade C; grade A versus grade C, p = 0.003). CONCLUSION: The elevation of SBF may play an important role in the development of hypersplenism and disorders in vasoactive factors such as the serum NO caused by liver cirrhosis may play an important role in the elevation of SBF.


Subject(s)
Hypersplenism/physiopathology , Splenic Artery/physiology , Adult , Aged , Blood Flow Velocity/physiology , Case-Control Studies , Female , Hepatitis B/complications , Humans , Hypersplenism/blood , Hypersplenism/diagnostic imaging , Hypersplenism/etiology , Liver Cirrhosis/virology , Male , Middle Aged , Nitric Oxide/blood , Platelet Count , Severity of Illness Index , Ultrasonography, Doppler
14.
Int J Colorectal Dis ; 25(2): 267-75, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19924422

ABSTRACT

PURPOSE: The aim of this study was to estimate efficacy of mechanical bowel preparation with polyethylene glycol (PEG) in prevention of postoperative complications in elective colorectal surgery. METHOD: A literature search of MEDLINE (PubMed), EMBASE, and the Cochrane Library was done to identify randomized controlled trials involving comparison of postoperative complications after mechanical bowel preparation with PEG (PEG group) and no preparation (control group). A meta-analysis was set up to distinguish overall difference between the two groups. RESULTS: A total of five randomized controlled trials was identified according to our inclusion criteria. The use of PEG for mechanical bowel preparation did not significantly reduce the rate of surgical site infection (SSI; odds ratio (OR) 95% confidence interval (CI), 1.39 (0.85-2.25); P = 0.19) including incisional SSI (OR 95% CI, 1.44 (0.88-2.33); P = 0.15), organ/space SSI (OR 95% CI, 1.10 (0.43-2.78); P = 0.49), anastomotic leak (OR 95% CI,1.78 (0.95-3.33; P = 0.07), mortality (OR 95% CI, 1.24 (0.37-4.14; P = 0.73), infectious complications (OR 95% CI, 1.14 (0.62-2.08); P = 0.67), and hospital stay (weighted mean difference 95% CI, 2.17 (-2.90-7.25); P = 0.40) except main complications (OR 95% CI, 1.76 (1.09-2.85); P = 0.02), of which the rate increased significantly in the PEG group. CONCLUSION: The use of mechanical bowel preparation with PEG does not significantly lower postoperative complications in elective colorectal surgery.


Subject(s)
Colon/surgery , Digestive System Surgical Procedures/adverse effects , Polyethylene Glycols/therapeutic use , Postoperative Complications/prevention & control , Rectum/surgery , Chi-Square Distribution , Digestive System Surgical Procedures/mortality , Elective Surgical Procedures , Humans , Length of Stay , Odds Ratio , Polyethylene Glycols/adverse effects , Postoperative Complications/mortality , Preoperative Care , Risk Assessment , Surgical Wound Infection/etiology , Surgical Wound Infection/prevention & control , Treatment Outcome
15.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-297762

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the clinical effects of Simo Decoction Oral Liquid for the treatment of gastrointestinal dysfunction after stable thoracolumbar fractures.</p><p><b>METHODS</b>From May 2005 to July 2008, 81 patients with stable thoracolumbar fractures were randomly divided into treatment group (41 cases) and control group (40 cases) according to a random digits table. The treatment group included 32 males and 9 females with an average age of (47.19 +/- 5.18) years old ranging from 21 to 55 years, and the course was from 1 to 45 hours with an average of (7.83 +/- 1.29) hours. The control group included 30 males and 10 females with an average age of (46.31 +/- 3.72) years ranging from 20 to 54 years,and the course was from 1.5 to 43 hours with an average of (8.15 +/- 1.63) hours. The treatment group were dealed with Simo Decoction Oral Liquid,and the control group with neostigmine for acupoint block in bilateral Foot-Three-Li. The recovery of gastrointestinal function and the first passage of gas by anus were compared.</p><p><b>RESULTS</b>The time of recovery of gastrointestinal function in treatment group (7.27 +/- 3.14) h was shorter than that in control group (10.12 +/- 3.62) h. The time of first passage of gas by anus in treatment group (15.39 +/- 13.70) h was significantly shorter than that in contral group (24.02 +/- 18.11) h. The total effective rate in treatment group was higher than that in control group.</p><p><b>CONCLUSION</b>Both the treatment group and the control group have clinical effects in treatment of the restoration of gastrointestinal dysfunction after the stable thoracolumbar fractures, but the treatment group has more remarkable therapeutic effect and less side effects.</p>


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Gastrointestinal Diseases , Drug Therapy , Gastrointestinal Motility , Lumbar Vertebrae , Wounds and Injuries , Medicine, Chinese Traditional , Spinal Fractures , Thoracic Vertebrae , Wounds and Injuries
16.
J Phys Chem A ; 113(47): 13407-12, 2009 Nov 26.
Article in English | MEDLINE | ID: mdl-19438219

ABSTRACT

We report on the first detailed studies of the spectroscopy of an iodocarbene, measuring fluorescence excitation and emission spectra of the X1A' --> A1A'' system of :CHI and the deuterated isotopomer :CDI. Due to similar bending and C-I stretching frequencies in the upper state, fluorescence excitation spectra of :CHI show polyads composed of members of the 2(0)(n-x)3(0)x progressions with x = 0-3. For :CDI, only progressions with x = 0, 1 are observed. Extrapolation of the 20n term energies for both isotopomers to a common origin places the electronic origin of the X1A' --> A1A'' system near 10500 cm-1, in good agreement with theoretical predictions. Rotational analysis of the 16 observed bands for CHI and 13 observed bands for :CDI yields rotational constants for the upper and lower states that are also in good agreement with theory. To investigate the controversial issue of the ground state multiplicity of :CHI, we measured single vibronic level emission spectra from many A1A'' levels. These spectra show conclusively that the ground state is a singlet, as for both isotopomers the ã3A'' origin is observed, lying well above the origin of the X1A' state. At energies above the ã3A'' origin, the spin-orbit mixing is so severe that few vibrational assignments can be made. Analysis of the emission spectra provides a lower limit on the singlet-triplet gap of 4.1 kcal mol-1, in excellent agreement with theoretical predictions.

17.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-389623

ABSTRACT

Objective To explore the endogenesis metabolizer character of kidney-yang insufficiency syndrome model rats caused by hydrocortisoni natrii succinas with metabolomics technology. Methods Twenty four Sprague-Dawley(SD)male rats were divided randomly into a normal group, a kidney-yang insufficiency model group, and a oral administration group, eight rats in each group. After producing kidney-yang insufficiency model by injecting hydrocortisoni natrii succinas intramuscularly, oral administration group rats were administered orally with white prepared lateral root of aconite every day for two weeks. After twenty-four hour the last oral administration, the blood plasm were prepared and used for testing endogenesis metabolism with the liquid phase color spectrum-mass spectra (LC/MS) metabolomics technology. Results Apices shape change of lysophosphatidyl choline (LPC) and lysophosphatidyl ethanolamine(LPE) and 468.4m/z unbeknown chemical compound of normal rats were distinct from those of model rats. Above-mentioned chemical compound as chief material symbols of kidney-yang insufficiency syndrome might be farther studied. Conclusion Finding out differential symbol from endogenesis metabolizer with metabolomics technology was redounded to deep exploring the biology essence of traditional Chinese medicine syndrome.

18.
J Chem Phys ; 129(10): 104309, 2008 Sep 14.
Article in English | MEDLINE | ID: mdl-19044915

ABSTRACT

We report on high resolution studies of spin-orbit mixing and the singlet-triplet gap in a prototypical halocarbene, CHCl, using stimulated emission pumping (SEP) spectroscopy from the A (1)A(") state. Results are reported for two isotopomers, CH(35)Cl and CD(35)Cl. We have obtained rotationally resolved spectra for the majority of X (1)A(') levels lying between 0 and 6000 cm(-1) above the zero-point level that were previously observed under low resolution in single vibronic level emission studies and several new levels that were previously unobserved or unresolved. In addition, SEP spectra were obtained for six a (3)A(") levels in CH(35)Cl and three levels in CD(35)Cl. The derived term energies and rovibrational parameters of the X (1)A(') and a (3)A(") states are in good agreement with theory. The a (3)A(") triplet spin-spin parameter is vibrational state dependent, and dominated by a second-order contribution from spin-orbit coupling with nearby X (1)A(') levels; it therefore provides a sensitive probe of spin-orbit mixing in this system. An analysis of three pairs of interactions between specific a (3)A(") and X (1)A(') levels in CH(35)Cl affords a pure electronic spin-orbit coupling element of 150 cm(-1), in good agreement with theoretical expectations. The derived singlet-triplet gaps, which are the most precise determined to date for any carbene, are compared with the predictions of high level ab initio theory.

19.
Phys Chem Chem Phys ; 10(40): 6090-2, 2008 Oct 28.
Article in English | MEDLINE | ID: mdl-18846296

ABSTRACT

We report the first observation of iodocarbene (CHI) using fluorescence excitation and emission spectroscopy, which indicate a singlet ground state and a lower bound to the singlet-triplet gap of 3.76 kcal mol(-1).

20.
J Phys Chem A ; 112(45): 11355-62, 2008 Nov 13.
Article in English | MEDLINE | ID: mdl-18925733

ABSTRACT

We report studies aimed at unraveling the complicated structure of the CCl 2 A (1)B 1 <-- X (1)A 1 system. We have remeasured the fluorescence excitation spectrum from approximately 17,500 to 24,000 cm (-1) and report the term energies and A rotational constants of many new bands for both major isotopologues (C (35)Cl 2, C (35)Cl (37)Cl). We fit the observed term energies to a polyad effective Hamiltonian model and demonstrate that a single resonance term accounts for much of the observed mixing, which begins approximately 1300 cm (-1) above the vibrationless level of the A (1)B 1 state. The derived A (1)B 1 vibrational parameters are in excellent agreement with ab initio predictions, and the mixing coefficients deduced from the polyad model fit are in close agreement with those derived from direct fits of single vibronic level (SVL) emission intensities. The approach to linearity and thus the Renner-Teller (RT) intersection is probed through the energy dependence of the A rotational constant and fluorescence lifetime measurements, which indicate a barrier height above the vibrationless level of the X (1)A 1 state of approximately 23,000-23,500 cm (-1), in excellent agreement with ab initio theory.

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