ABSTRACT
Medicinal plants effect on antilysozyme activity of E. coli, Klebsiella, S. aureus, Candida, Bifidobacterium, Bacteroides, Clostridium was evaluated in vitro. It was demonstrated that the effects differed substancially according to the microorganism taxonomy. The experimental data on the plants inhibitory activity may be used for elaboration of effective regimes of infections and disbiosis treatment.
Subject(s)
Bacteria/drug effects , Fungi/drug effects , Muramidase/antagonists & inhibitors , Plants, Medicinal , Bacteria/classification , Bacteria/isolation & purification , Bacterial Physiological Phenomena/drug effects , Fungi/isolation & purification , Fungi/physiology , Humans , Intestines/microbiology , Microbiological Techniques , Plant Preparations/pharmacologyABSTRACT
The effect of the duodenal contents and its components on the antilysozyme activity (ALA) of enterobacteria was studied. Under the action of native bile and the preparation of dried bile the stimulation of the expression of ALA factor in 86.6-93.3% of enterobacteria was observed. Pancreatin increased the level of the secretion of ALA factor by 50% in comparison with the control. The preparation of egg lysozyme produced no changes in the frequency and level of the expression of ALA factor. The study revealed the dose-dependant character of the action of duodenal contents: in the native form, as well as at concentrations of 50% and 25%, duodenal contents stimulated the expression of the ALA factor of enterobacteria; at concentrations of 12.5% and lower, its effect could be stimulating, inhibitory or neutral.
Subject(s)
Enterobacteriaceae/metabolism , Enzyme Inhibitors/metabolism , Gastrointestinal Tract/metabolism , Bile Acids and Salts/pharmacology , Dose-Response Relationship, Drug , Enterobacteriaceae/drug effects , Enzyme Inhibitors/analysis , Humans , Muramidase/antagonists & inhibitors , Muramidase/metabolism , Pancreatin/pharmacologyABSTRACT
Methods for the rapid and immediate determination of the anti-interferon activity of bacteria have been developed. The use of these methods makes it possible to reduce the time of determination from 48 hours to 24 and 7 hours. The proposed methods require no additional production costs, while their sensitivity and specificity are not inferior to those ensured by the classical method. These methods are recommended for the etiological diagnosis of diseases caused by opportunistic enterobacteria.