ABSTRACT
Prospects for the use of chromosomal transformation for the identification of Pseudomonas pseudomallei and some other medically important Pseudomonas species are considered. The method is simple, reproducible and highly specific. It permits the screening of a great number of cultures and can facilitate further study of the biology of P. pseudomallei and the epidemiology of melioidosis.
Subject(s)
Chromosomes, Bacterial/ultrastructure , Melioidosis/microbiology , Pseudomonas/genetics , Transformation, Genetic , Animals , Bacteriological Techniques , DNA, Bacterial/genetics , Genetic Markers , Genetic Techniques , Humans , Pseudomonas/isolation & purification , Pseudomonas/ultrastructureSubject(s)
Culture Media/pharmacology , Transformation, Bacterial/drug effects , Bacteria/genetics , Bacteria/isolation & purification , Binding, Competitive , Calcium/pharmacology , DNA, Bacterial/genetics , Deoxyribonucleases/genetics , Drug Resistance, Microbial , Genetic Techniques , Mutation , Receptors, Cell Surface/geneticsABSTRACT
The mapping of the chromosome sections in P. pseudomallei, responsible for the synthesis of histidine, has been carried out with the use of two- and three-factor crossing. Two new groups of marker linkage have been found: his 522 with ilv 658 and his 37 with asp 37 glu 37. The linkage of some of the existing histidine mutations with locus his 37 has been revealed and the relative position of the markers in this linkage group has been shown.
