ABSTRACT
Norepinephrine increases the intracellular level of adenosine 3',5'-monophosphate (cyclic AMP) in clonal cell lines of nerve, glia, smooth muscel, and skeletal muscle. The largest response is in skeletal muscle, where the cyclic nucleotide concentration is elevated more than 500-fold. Glia and muscle cells, but not nerve cells, respond to dopamine with increased cyclic AMP accumulation. This response appears to be mediated through a beta-adrenoreceptor.
Subject(s)
Cyclic AMP/metabolism , Muscles/metabolism , Neuroglia/metabolism , Neurons/metabolism , Cell Line , Receptors, AdrenergicSubject(s)
Acetylcholine/biosynthesis , Aminobutyrates/biosynthesis , Cell Division , Clone Cells , Dopamine/biosynthesis , Neurons/metabolism , gamma-Aminobutyric Acid/biosynthesis , Acetyltransferases/metabolism , Animals , Carboxy-Lyases/metabolism , Cell Differentiation , Choline , Glutamates , Neurons/enzymology , Rats , Synaptic Transmission , Time Factors , Tyrosine 3-Monooxygenase/metabolismABSTRACT
The regulation of the specific activities of adenylate kinase (EC 2.7.4.3) and creatine kinase (EC 2.7.3.2) in myogenic cell lines is independent of cell fusion. The observed increases in enzyme specific activities are cell density dependent, and may be further broken down into contributions from an increase in enzyme activity per cell and a decrease in protein per cell. Only the former appears to be affected by medium conditioning.
Subject(s)
Creatine Kinase/metabolism , Muscles/embryology , Phosphotransferases/metabolism , Animals , Cell Count , Cell Differentiation , Cell Fusion , Cell Line , Muscles/enzymology , RatsSubject(s)
Cell Line , Clone Cells , Neoplasms, Nerve Tissue/chemically induced , Neuroglia/metabolism , Neurons/metabolism , Aminobutyrates/analysis , Animals , Bucladesine/pharmacology , Cell Division , Cell Membrane , Culture Media , Neoplasms, Experimental/chemically induced , Neoplasms, Experimental/classification , Neoplasms, Nerve Tissue/classification , Nerve Tissue Proteins/analysis , Neuroblastoma , Neuroglia/analysis , Neuroglia/drug effects , Neuroglia/enzymology , Neurons/analysis , Neurons/drug effects , Neurons/enzymology , Neurotransmitter Agents/biosynthesis , Nitrosourea Compounds , Rats , Synaptic TransmissionSubject(s)
Acetylcholine/biosynthesis , Neuroblastoma/metabolism , Pyridinium Compounds/pharmacology , Acetylcholine/antagonists & inhibitors , Acetyltransferases/antagonists & inhibitors , Animals , Carbon Radioisotopes , Choline/analysis , Clone Cells , Culture Media , Electrophoresis, Paper , Mice , Muscles/metabolism , Naphthalenes/pharmacology , Time Factors , Vinyl Compounds/pharmacologySubject(s)
Muscle Proteins/biosynthesis , Muscles/metabolism , Animals , Carbon Radioisotopes , Cell Differentiation , Cell Line , Clone Cells , Collagen/metabolism , Creatine Kinase/metabolism , Electrophoresis, Polyacrylamide Gel , Hydroxyproline/metabolism , In Vitro Techniques , Leucine/metabolism , Muscle Proteins/metabolism , Muscles/embryology , Muscles/enzymology , Myofibrils , Myosins/biosynthesis , Myosins/metabolism , Phosphotransferases/metabolism , Proline/metabolism , Rats , Time Factors , TritiumSubject(s)
Antigen-Antibody Reactions , Haptens , Multiple Myeloma/immunology , Neoplasm Proteins , Absorption , Animals , Antibody Specificity , Binding Sites , Carbon Isotopes , Chromatography , Chromatography, Gel , Cross Reactions , Dialysis , Dinitrophenols , Filtration , Immune Sera , Immunochemistry , Immunodiffusion , Immunoglobulin A/analysis , Membranes, Artificial , Mice , Neoplasm Proteins/analysis , Protein Denaturation , Tritium , Trypsin/pharmacologyABSTRACT
Cytoplasmic particles which sedimented at 150S were the smallest structures containing detectable viral ribonucleic acid polymerase in mouse cells infected with encephalomyocarditis virus.
