Metal contamination in sediments of dam reservoirs: A multi-facetted generic risk assessment.

The quality of bottom sediments is a key factor for many functions of dam reservoirs, which include water supply, flood control and recreation. The aim of the study was to combine different pollution indices in a critical generic risk assessment of metal contamination of bottom sediments. Both geochemical and ecological indices reflected that sediment contamination was dominated by Zn, Pb and Cd. The ecological risk indices suggested a high riks for all three metals, whereas human health risks were high for Pb and Cd. An occasional local contamination of sediments with Cr and Ni was revealed, although at levels not expected to cause concerns about potential ecological or health risk. Sediments from the Rybnik reservoir for Cu only revealed a high potential ecological risk. EF turned to be as being the most useful, whereas TRI (∑TRI) was the most important ecological index. All multi-element indices suggested similar trends, indicating that Zn, Pb and Cd taken altogether had the greatest impact on the level of sediment contamination and posed the greatest potential ecological and health risks to organisms. The use of sequential BCR extraction and ecotoxicity analyses allowed for a multi-facetted generic risk assessment of metals in sediments of dam reservoirs.

Immobilisation of metals from bottom sediments using two additives and thermal treatment.

A major problem associated with the land-based management of bottom sediments is their contamination with metals. The aims of the study were: 1) to use two additives for the immobilisation of metals; and 2) to evaluate the effect of three combustion temperatures on metal content and bottom sediment properties. The mixtures were prepared using contaminated bottom sediment and the following waste materials: cellulosic waste and biomass ash. In the second experiment, the bottom sediment samples were subjected to a thermal process, and three temperatures were chosen 500/800/950 °C. Overall, the addition of cellulosic waste and biomass ash to acidic, metal-contaminated bottom sediments significantly improved the properties of the resulting mixtures, including an increase in the pH value, sorption capacity, macronutrient content, and a decrease in the content and mobility of metals (Cd, Zn, Pb, Cr). The study confirmed the effectiveness of the thermal process on a significant reduction in the ecotoxicity of the sediments, a reduction in total content of elements, and a decrease in their leachability, and thus mobility, with increasing process temperature. The study results revealed that the converted contaminated bottom sediments can be effectively managed, provided that further studies on their technical application are carried out.

Concentration of dioxin and screening level ecotoxicity of pore water from bottom sediments in relation to organic carbon contents.

The information about concentrations of dioxin in pore water, ecotoxicity and DOC and TOC content can be key factor for the prediction of the fate of dioxins in the aquatic environment as well as an ecological risk assessment. The aims of the study were to assess the concentration of PCDDs/PCDFs and ecotoxicity of pore water and to compare above results in relation to the dissolved organic carbon (DOC) and total organic carbon (TOC) content. The concentration of dioxins was assessed using an enzyme-linked immunoassay test, while the ecotoxicity of pore water was determined using a crustacean Daphnia magna and bacteria Aliivibrio fischeri. The studies were conducted on two different dammed reservoirs Roznów (catchment basin of an agricultural character) and Rybnik (catchment basin of an industrial character) located in southern Poland. The concentration of dioxins in pore water was between 8.56 to 90.92 ng EQ/L, with a significantly higher concentration in the pore water from the Roznów Reservoir than the Rybnik Reservoir. The DOC content in pore water was from 30.29 to 63.02 mg/L (Roznów Reservoir) and from 35.46 to 60.53 mg/L (Rybnik Reservoir). Higher toxic responses were recorded for A. fischeri than for D. magna. Moreover a significantly higher toxicity for both tested organisms was indicated in pore water from the Roznów Reservoir. Besides of TOC and DOC, the fine fractions of the sediments were particularly important in the concentration of dioxin in pore water. The other pore water parameters, such as pH and EC can influence the toxicity of water for organisms. The result indicate complex relationships between the PCDD/F, ecotoxicity and DOC, TOC concentration in pore water and confirms that these parameters are important in terms of water environmental contamination.

Ecotoxicological and chemical properties of the roznów reservoir bottom sediment amended with various waste materials.

In the study, an attempt was made to create innovative mixtures based on bottom sediment and various types of waste to be tested for use as a substrate in the cultivation of consumer and non-consumer plants. The aim of the study was to assess the chemical and ecotoxicological properties of the growing medium prepared on the basis of bottom sediment (BS) and different carbon rich waste (cellulose waste (CW), biomass ash (BA), coffee hulls (CH), and sludge from water treatment (SW)) with a combination of 75% bottom sediment and 25% waste material. The mixtures had deacidifying properties, significant content of total organic carbon (TOC), the total quantities of macro- and micronutrients, and good sorption properties. The study showed a low total content of heavy metals in the mixtures. Moreover, a low share of the mobile fraction (F1) of metals indicated a low risk related to the metals mobility and potential bioavailability. The highest toxic effect was found in the mixture of bottom sediment and cellulose waste. Heterocypris incongruens was the most sensitive organism to substances present in the tested mixtures. Due to its low toxicity, the mixture of bottom sediment and water treatment sludge (BS+SW) constituted a potentially suitable substrate for its environmental application in agriculture, horticulture (for consumer crops) or land reclamation. Other mixtures exhibited valuable chemical properties (BS + BA, BS + CH BS + CW), but ended up being eco-toxic to the organisms, excluding them from agricultural or horticultural use for consumer crops. The proposed technology, which includes the production of growing medium based on the use of bottom sediment, is a promising way of transforming the sediment from a waste material to a valuable resource, thus enhancing the environmental benefits.

Ecotoxicological characteristics and ecological risk assessment of trace elements in the bottom sediments of the Roznów reservoir (Poland).

The bottom sediments in catchment areas behind dams play a significant role in water ecosystems. On the other hand, the structure of sediments makes them a natural geosorbent, in which pollutants introduced to the aquatic environment accumulate. The use of biotests is recognised as an important approach for the assessment of the quality of bottom sediments, as the chemical analysis of sediment samples alone does not provide evidence of the impact of contaminants on biota. The aim of the study was to apply the chemical and ecological indices to determine the potential risk posed by trace elements in the bottom sediments and to evaluate sediment toxicity using organisms belonging to two taxonomic groups, i.e., plants (Phytotoxkit) and crustaceans (Rapidtoxkit). The 46 sediment samples were taken from the Roznów Dam Reservoir in Southern Poland. The mean concentration of the trace elements in the sediments was 5.22 mg As; 0.26 mg Cd; 63.23 mg Cr; 28.65 mg Cu; 37.11 mg Ni; 11.15 mg Pb; 69.69 mg Zn and 0.09 mg Hg ∙ kg-1 d.m. The mean probable effect concentration quotient (PECq) value among different sampling sites ranged between 0.04 and 0.33 suggested moderate potential toxicity to the biological communities in bottom sediments. The Ni was potentially the most toxic element for biota in the Roznów Reservoir. The sensitivity of organisms formed the following order: Thamnocephalus platyurus >Lepidium sativum >Sinapis alba >Sorghum saccharatum. For the plants, the stimulating effect of bottom sediments on root growth was often indicated, while a toxic effect was demonstrated for T. platyurus in 80% of the samples. However, the correlation analysis and PCA results showed that trace elements that originated from similar sources were associated to the toxicity of sediments towards T. platyurus, while ecotoxicity for plants could not be explained by the content of trace elements in bottom sediments. T. platyurus is a good indicator for predicting the toxicity of bottom sediments from the Roznów Reservoir. However, our study found that both chemical and ecotoxicological analyses are important for a comprehensive evaluation of the quality of bottom sediments.

Content of nutrients, trace elements, and ecotoxicity of sediment cores from Roznów reservoir (Southern Poland).

The aims of the study were to investigate the concentration of trace elements, nutrients, and ecotoxicity in bottom sediment cores collected from the silted part of the Roznów reservoir (Southern Poland). Significant differences in the content of nutrients, trace elements, and ecotoxicity between five sediment cores were found. However, in the vertical distribution, there was no high variability of the above parameters, which means that the intensely suspended matter transported by the Dunajec river is and, at various times, has been homogeneous. Significant correlations between nutrients and trace elements (r = 0.33-0.91, at p ≤ 0.05) point to the same sources of the above-mentioned substances and similar levels of contamination in the sediment cores. However, the PCA results showed that cadmium and phosphorus in the sediment cores had different behaviors than other elements and can be associated mainly with anthropogenic sources. According to the degree of contamination factor, sediment cores fall under the category of considerable contamination of metals. Geochemical factors indicated that nickel, chromium, and cadmium (only sediment core C1) were found to be the cause of significant pollution in the sediment cores. Toxicity assessment found that most of the bottom sediment samples were classified as non-toxic or slightly toxic, only 10% of the sediment samples were toxic for Vibrio fischeri, and 6% of the samples were toxic for Sinapis alba. The two test organisms showed a different sensitivity, and higher toxic responses were recorded for V. fischeri than for S. alba. Cadmium and phosphorus were associated with toxicity for S. alba (r = 0.29-0.58, at p ≤ 0.05), whereas TOC, N, and S, and Ca for stimulation of growth this plants. Trace elements (r = 0.32-0.51, at p ≤ 0.05) and nutrients (S, K, Mg, Na, r = 0.44-0.58, at p ≤ 0.05) were positively correlated with inhibition of luminescence of V. fischeri. The studies of concentration and relation between trace elements, nutrients, and ecotoxicity are important in the ecological risk assessment and describing the quality of sediments with multiple sources contamination.

The influence of the quantity and quality of sediment organic matter on the potential mobility and toxicity of trace elements in bottom sediment.

Knowledge on the fraction of trace elements in the bottom sediments is a key to understand their mobility and ecotoxicological impact. The purpose of this study was to assess the influence of the content of organic matter fractions on the mobility and ecotoxicity of trace elements in sediments from the Rybnik reservoir. The most refractory fraction of organic matter-Cnh (non-hydrolysing carbon)-dominated in the sediments. The content of organic matter fractions are arranged in the following order: Cnh (non-hydrolysing carbon) > Cfa (fulvic acid) > Cha (humic acid) > DOC (dissolved organic carbon). On the other hand, the highest value of correlation coefficients was found for different fractions of trace elements and DOC content in the bottom sediments. A higher content of TOC in the sediments significantly increased the share of elements in the potential mobile fraction and, at the same time, decreased the binding of elements in the mobile fractions. Moreover, in sediments that contain more than 100 g/kg d.m. TOC, no and medium risk of trace element release from sediments was observed. The Cu, Cd and Ni were potentially the most toxic elements for biota in the Rybnik reservoir. However, the correlation between the content of trace elements and the response of bacteria was insignificant. These results suggested that the complexation of trace elements with organic matter makes them less toxic for Vibrio fischeri. The transformation and sources of organic matter play an important role in the behaviour of trace elements in the bottom sediments of the Rybnik reservoir.

Use of Chemical Indicators and Bioassays in Bottom Sediment Ecological Risk Assessment.

This study is was designed to assess the ecological risk associated with chemical pollution caused by heavy metals and PAHs on the basis of their ecotoxicological properties in sediments collected from the Rzeszów dam reservoir (Poland). The sediment samples were collected from three sampling stations: S1-inlet, backwater station, S2-middle of reservoir, S3 outlet, near the dam. The sediments' toxicity was evaluated using a battery of bioassays (Phytotoxkit, Phytotestkit, Ostracodtoxkit F, and Microtox). The highest content of metals (120.5 mg Zn; 22.65 mg Pb; 8.20 mg Cd ∙ kg-1 dw) and all PAHs (∑9361 µg ∙ kg-1 dw) in sediments was found at station S1. The lowest content of metals (86.72 mg Zn; 18.07 mg Cu; 17.20 mg Pb; 3.62 mg Cu; 28.78 mg Ni; 30.52 mg Cr ∙ kg-1 dw) and PAHs (∑4390 µg ∙ kg-1 dw) was found in the sediment from station S2. The ecological risk assessment of the six metals and eight PAHs revealed a high potential toxicity in sediments from stations S1 (PECq = 0.69) and S3 (PECq = 0.56) and a low potential toxicity in sediments from station S2 (PECq = 0.38). The studies also showed the actual toxicity of sediments for the test organisms. The sediment pore water was least toxic compared to the whole sediment: solid phases > whole sediment > pore water. The most sensitive organism for metals and PAHs in bottom sediments was Lepidium sativum, and in pore water-Sorghum saccharatum. The concentration of metals and PAHs in bottom sediments generally did not affect the toxicity for other organisms. Clay content and organic C content are likely to be important factors, which control heavy metal and PAH concentrations in the sediments. Data analysis by PCA found the same origin of metals as well as PAHs-mainly anthropogenic sources. The obtained information demonstrated the need to integrate ecotoxicological and chemical methods for an appropriate ecological risk assessment.

Concentration, sources and risk assessment of PAHs in bottom sediments.

The aims of the study were to investigate the concentration, sources and ecological risk of PAHs (polycyclic aromatic hydrocarbons) in bottom sediments collected from nine reservoirs located in south-eastern Poland. The concentration of ∑PAHs in sediments ranged from 150 to 33,900 µg kg-1. The total PAH concentration in the bottom sediments was arranged in the following order: Rybnik > Rzeszów > Brzóza Królewska > Brzóza Stadnicka > Besko > Chechlo > Ozanna > Gluchów > Narozniki. BAP was the major compound in sediments from the Besko, Brzóza Stadnicka and Rzeszów reservoirs; FLT in the sediments from the Rybnik, Narozniki, Ozanna and Brzóza Królewska reservoirs; and FLN from the Gluchów and Chechlo reservoirs. The major inputs of PAHs were of pyrolytic origin. However, petrogenic sources of PAHs occurred especially in the Chechlo and Gluchów reservoirs. The ecological risk assessment indicated that non-adverse effects on the benthic fauna may occur for sediments from the Gluchów, Narozniki and Ozanna reservoirs, while slightly adverse effects were found for sediments from the Brzóza Królewska, Besko, Brzóza Stadnicka and Chechlo reservoirs. The other sediments showed moderate (Rzeszów reservoirs) and strong effect (Rybnik reservoir) on biological communities. Individual PAHs such as NAP, PHE, FLT, PYR, BAA, CHR and BAP in sediments from the Rybnik reservoir and BAP in sediments from the Rzeszów reservoirs indicated a higher possibility of occurrence of an adverse ecological effect. PCA analysis found slight difference between the reservoirs in the profile of variable PAHs. Only the sediments from the Rybnik and Chechlo reservoirs differ considerably from this grouping.

Spatial distribution of trace elements and ecotoxicity of bottom sediments in Rybnik reservoir, Silesian-Poland.

The aim of study was to integrate chemical analyses and toxicity bioassays in order to assess the environmental risk connected with the presence of trace elements in the sediments. This study examined the ecological significance of trace elements in bottom sediments by applying a set of complementary sediment quality assessment methods sediment quality guidelines (SQGs) (mean probable effect concentration quotient (PECQ)), potential ecological risk index (PERI), contamination degree (C d) and two bioassays: the bacterial luminescence inhibition test with Vibrio fischeri on sediment elutriates and the direct contact test with the ostracod crustacean Heterocypris incongruens. The samples were collected from 50 stations of Rybnik reservoir. The reservoir is a region with enormous concentration of industry, mainly hard coal mining, electric power industry, and transportation. Despite the high diversity in metal concentration in the sediments, the spatial distribution of trace elements in the sediments was very similar. Moreover, the strong positive correlations between individual pairs of trace elements indicate that they may derive from a similar source and move together. According to mean PECQs, 68 % of the samples were potentially non-toxic and 32 % of the samples were potentially toxic. PERI values suggested that 70 % of the sediment sampling sites exhibited low ecological risk from metal pollution while 24 % of the samples had severe and serious risk. Based on our combined evaluation, we believe that Cd and Cu in the sediment samples frequently caused adverse biological effects. Higher toxic responses were observed in the Microtox test than in the Ostracodtoxkit test. All the sediment samples were found toxic to V. fischeri, and 96 % of the samples had effect percentages >50 %. For H. incongruens, 12 % of the sediments were not toxic and 44 % had effect percentages >50 %. In order to perform a complex assessment of the environmental impact of metal pollution, both chemical and ecotoxicological analysis should be carried out.

Assessment of heavy metals mobility and toxicity in contaminated sediments by sequential extraction and a battery of bioassays.

The aim of this study was to assess heavy metals mobility and toxicity in sediments collected from a dam reservoir in the conditions of intensive human impact by using chemical fractionation and a battery of bioassays. In the studies, the test organisms were exposed to substances dissolved in water (Microtox, Phytotestkit) as well to substances absorbed on the surface of solid particles (Phytotoxkit, Ostracodtoxkit F). The studies showed that sediments from the Rybnik reservoir are toxic, but the tested organisms showed different sensitivity to heavy metals occurring in the bottom sediments. The sediment samples were classified as toxic and very toxic. Moreover, the studies showed a higher toxicity in solid phases and whole sediment than in pore water. The lowest sensitivity was observed in H. incongruens (solid phases) and V. fischeri (pore water, whole sediment). The studies revealed that the toxicity of the sediments is caused mainly by heavy metal forms associated with the solid phase of the sediments. The studies did not confirm the metals occurring in fraction I (exchangeable) to be bioavailable and toxic to living organisms because most correlations between the metal concentration in fraction I and the response of the organisms were negative. The highest mobility from the bottom sediments was found in zinc, average mobility--in copper, cadmium and nickel, and low mobility--in chromium and lead. Organic matter is likely to be the most important factor controlling metal distribution and mobility in the studied sediments.

Phytotoxkit/Phytotestkit and Microtox® as tools for toxicity assessment of sediments.

The aim of the study was to use bioassays to evaluate the toxicity of pore water, sediments and sediments elutriates. Furthermore, a possible relationship between observed toxicity and results of chemical analysis was examined. Sediment (0-10 cm) samples were collected from 21 locations in Zeslawice reservoir, Southern Poland using an Ekman sampler. Toxicity assessment of the sediment and pore water samples was performed using direct-contact tests Phytotoxkit/Phytotestkit and Microtox®. Inhibition of seed germination in the test plants was from -25 to 38 percent for sediment and from 0 to 50 percent for pore water, whereas inhibition of root growth was within a range from -42 to 37 percent for sediment and from -49 to 37 percent for pore water. Depending on the sediments, Vibrio fischeri luminescence inhibition was from -18 to 40 percent for sediments elutriates and from -12 to 28 percent for pore water. The toxicity tests showed a positive correlation between metals and the root growth inhibition in Lepidium sativum and Sinapis alba as well as the luminescence inhibition in V. fischeri. No significant correlations were found between the inhibition of luminescence and the phytotoxicity assays, so these analyses do not show a similar sensitivity to toxicants in the sediments. While estimating the sensitivity of the performed biotests, the highest number of toxic responses was recorded in the Microtox® test towards V. fischeri. Among the plant species, Sorghum saccharatum appears to be the most sensitive plant species. Most of the examined sediment samples (67 percent) were classified as class II (low-toxic samples, low acute hazard) and 33 percent of the examined samples were class I (no essential toxic effect, non-toxic sample, no acute hazard) in terms of toxicity. Most of the pore water samples (71 percent) were also classified as class II.