Peripheral blood stem cell collection using the small volume collection chamber in the Fenwal CS-3000 Plus blood cell separator.

Optimal methods for peripheral blood stem cell (PBSC) collection should yield a small volume product containing minimal platelets and a large number of mononuclear cells (MNC). The Fenwal CS-3000 Plus blood cell separator was modified in an attempt to meet these objectives. Modifications of the CS-3000 Plus included use of the small volume collection chamber (SVCC), increasing the interface/offset detector setting to 150 and decreasing the centrifuge speed to 1400 rpm. Thirty-eight patients undergoing 224 PBSC collections were studied. Mobilization methods included 4 g/m2 cyclophosphamide (CY), CY + 250 micrograms/m2 subcutaneous granulocyte-macrophage colony-stimulating factor (GM-CSF) or GM-CSF alone. The median collection volume was 58 ml containing a median of 21 ml of red blood cells. Platelet collection efficiency was < 4.4% and the median number of extracted platelets was 0.6 x 10(11)/apheresis. Median reduction in the platelet count post-apheresis was 15%. MNC purity was 95.5% and MNC collection efficiency was 61%. Yield of MNC was 1 x 10(8)/kg/apheresis. Collected progenitor cells correlated with both the WBC and MNC content of the apheresis product. The modified CS-3000 Plus with the SVCC is effective for PBSC collection following three different mobilization regimens and is, therefore, recommended for routine collection of PBSC.

Inadequate red cell depletion of large-volume marrow harvests processed on the Fenwal CS-3000 Plus.

In this report we describe a procedure for the depletion of erythrocytes in bone marrow harvests using the Fenwal CS-3000 Plus cell separator. In a study of 15 consecutive harvests, adequate red cell depletion could not be achieved with large-volume marrows with > 500 ml erythrocytes.