ABSTRACT
The effect of feeding rats with high doses of vitamin A on the distribution of polycyclic hydrocarbon, 7,12-dimethylbenzanthracene (DMBA) and its metabolites in different organs and blood, and also on the metabolic rate in the liver was studied following intravenous injection of the carcinogen. Hypervitaminosis A resulted in a significant decrease of the level of DMBA and its metabolites in all the organs investigated and in the blood. The rate of DMBA metabolism in the liver of the animals rose with the increase of the vitamin A dose.
Subject(s)
Mammary Neoplasms, Experimental/chemically induced , Vitamin A/administration & dosage , 9,10-Dimethyl-1,2-benzanthracene/metabolism , Animals , Dose-Response Relationship, Drug , Female , Liver/metabolism , Mammary Neoplasms, Experimental/metabolism , Rats , Time FactorsABSTRACT
It was established that plant tissues killed by various aldehydes in low concentrations, which evoked general enzyme inactivation for a long time retain permeability and lipoprotein bonds in the cell membranes. Such preparations from Elodea Canadensis leaves are used for: Cu, Zn, Pb, Hg toxical effect investigation. It was stated by chemoluminescence methods that these ions induce slow developed oxidative radical chain reaction in lipid cell membranes and break the lipoprotein bonds.
Subject(s)
Metals/pharmacology , Plants/drug effects , Aldehydes , Fixatives , LuminescenceABSTRACT
The in vitro metabolism of 7,12-dimethylbenz(a)anthracene in its incubation with the liver microsomes, the liver and mammary gland homogenates of rats, kept on vitamin A-enriched diet, was studied. Vitamin A inhibited the formation of lipophilic metabolites and increased the generation of water-soluble metabolites. The amount of lipophilic metabolites extracted from the microsomes and the liver and mammary gland homogenates were decreased by a factor of 2.2 and 5, respectively. The amount of unmetabolized DMBA in the liver microsomes was the same in control and experimental animals.
Subject(s)
9,10-Dimethyl-1,2-benzanthracene/metabolism , Benz(a)Anthracenes/metabolism , Mammary Glands, Animal/metabolism , Microsomes/metabolism , Vitamin A/pharmacology , Animals , Female , Mammary Glands, Animal/ultrastructure , Microsomes, Liver/metabolism , RatsABSTRACT
Circadian rhythms of ultra-weak chemoluminescence of bean roots were investigated. It was found that under periodical change of light and darkness and without subsequent illumination a periodical change of spontaneous chemoluminescence of bean roots was observed. (The study of antiradical activity of the substances extracted from the root showed the dependence of this activity on illumination conditions.
Subject(s)
Circadian Rhythm , Luminescence , Plant Physiological Phenomena , Antioxidants/isolation & purification , Free Radicals , Plants/analysisABSTRACT
Mechanisms of formalin--induced luminescence of protein-lipid complexes have been studied. Free radical reactions of lipid oxidation were shown to be necessary for generating luminescence quanta. The luminescence intensity was mostly promoted by fluorescing products formed in the reoxidized system protein-lipid. Formalin-induced flash of luminescence in these systems may evidently serve as an express-method for analysing protein sample contamination with unsaturated lipids.
Subject(s)
Formaldehyde , Lipids , Proteins , Insulin , Linoleic Acids , Luminescence , Ovalbumin , Palmitic Acids , Serum Albumin , Serum Albumin, Bovine , TrypsinSubject(s)
9,10-Dimethyl-1,2-benzanthracene/metabolism , Benz(a)Anthracenes/metabolism , Lipid Metabolism , 9,10-Dimethyl-1,2-benzanthracene/analysis , Animals , Blood/metabolism , Chromatography, Gel , Chromatography, Thin Layer , Female , Kidney/metabolism , Kinetics , Liver/metabolism , Mammary Glands, Animal/metabolism , Rats , Spleen/metabolism , Time Factors , TritiumABSTRACT
The metabolism of 7,12-dimethylbenz(alpha) anthracene (DMBA) was studied, using labelled DMBA-H3, after intravenous injection of the carcinogen in female animals. The maximum excretion of the metabolites took place during the period ranging from 6 to 24 hours. The content of liposoluble metabolites in the urine was insignificant (about 6%), whereas in feces it was much higher (about 40%). Among the metabolites in feces the content of DMBA, 12-hydroxymethyl-7-methylbenz(alpha)anthracene and unidentified DMBA metabolite was significantly high. The content of 7-hydroxymethyl-12-methylbenz(alpha) anthracene fails to show any significant level.
