Expression of E-cadherin in drug resistant human breast cancer cells and their sensitivity to lymphokine-activated lymphocytes action.

AIM: To analyze the correlation between the elevated sensitivity of drug resistant breast cancer cells to the action of lymphokine-activated lymphocytes (LAK) and expression of E-cadherin and other marker proteins by cancer cells and lymphocytes. METHODS: Breast tumor explants were cultured with autologous lymphocytes in double diffusion chambers. The results were evaluated by morphological criteria of explants growth. Expression level of proteins on tumor cells was analyzed using immunohistochemical method on paraffin embedded sections, and by indirect immunofluorescence - on lymphocytes. RESULTS: Significant decrease of E-cadherin expression and significant increase of nuclear antigen of proliferating cells expression have been detected on drug resistant malignant human breast tumor (DRHBT) cells compared with drug sensitive breast tumor (DSHBT) cells. Autologous LAK possessed the highest antitumor activity against DRHBT cells that was associated with high expression level of soybean lectin receptor. CONCLUSION: Malignant drug resistant tumors are characterized by reverse relation between E-cadherin expression level and their proliferative activity. Marked antitumor action of LAK against these tumors is associated with high expression level of soybean lectin receptor on the lymphocytes.

[Disorders of hormonal homeostasis in patients with kraurosis and leukoplakia vulvae]. / Narusheniia gormonal'nogo gomeostaza u bol'nykh kraurozom i leikoplakiei vul'vvy.

A study was made into the functional status of hypophysis, adrenal cortex, ovaries, and thyroid gland in patients with kraurosis and leukoplakia vulvae. The above patients demonstrated profound disturbances in the system hypophysis-ovaries, that attest to the need for applying corrective therapies to treat the disturbed hormonal homeostasis.

[The endocrinological aspects of cancer of the vulva]. / Nekotorye éndokrinologicheskie aspekty raka vul'vy.

Patients with cancer of the vulva showed significant disorders of pituitary adrenal cortex, ovarian and thyroid function. This requires carrying out corrective hormone therapy with the purpose of increasing the efficiency of combined treatment.

[The treatment of edematous-infiltrative forms of breast cancer by using thermochemo- and thermoradiotherapy]. / Lechenie otechno-infil'trativnykh form raka molochnoi zhelezy s ispol'zovaniem termokhimio- i termoradioterapii.

The study included 157 patients with edematous-infiltrative breast cancer. Thermochemo- and thermoradiotherapy were shown to modify (increase) cancer cell sensitivity to antitumor treatment and thus improve tumor response.

[Experience with the use of a low-calorie diet in breast cancer patients to prevent metastasis]. / Opyt primeneniia nizkokaloriinoi diety u bol'nykh rakom molochnoi zhelezy s tsel'iu profilaktiki metastazirovaniia.

The paper discusses results of three-year follow-up of breast cancer patients keeping to a low-calorie diet after radical mastectomy and chemotherapy. The diet was planned according to age, energy expenditures and body weight and was aimed at a 15% decrease in the total caloricity as compared to corresponding individually-tailored norms for healthy people. This was achieved by reduction in fat (by 30%) and carbohydrates (by 9%). To assure the diet on the domiciliary basis, patients were provided with a table of nutrients for all food products available. Regular examination failed to show any adverse effects of the dietary restrictions on the general status of patients. However, an inhibiting effect of the diet on progression of disease was established, with recurrence being registered in 25% of controls (in 13 out of 52 patients) and only 7% of the study group (in 4 out of 56 cases).

[The determination of beta-casein gene expression and the assessment of hormonal homeostasis in gynecomastia patients and in breast cancer in men]. / Opredelenie ékspressii genov beta-kazeina i otsenka gormonal'nogo gomeostaza u bol'nykh s ginekomastiei i rakom molochnoi zhelezy u muzhchin.

beta-Casein genes expression in breast epithelium was studied in male patients with various forms of gynecomastia and cancer. Blood serum levels of pituitary, sex and glucocorticoid hormones were assayed in 29 patients with gynecomastia and 22 cases of breast cancer, and in 25 of them beta-casein genes expression was evaluated additionally. Activation of the above genes was established in the tissues studied. Their level proved to be in a correlation with that of prolactin.

[The function of the hypophysis, adrenal cortex and gonads in men with breast cancer]. / Funktsional'noe sostoianie gipofiza, kory nadpochechnikov i gonad u muzhchin, bol'nykh rakom molochnoi zhelezy.

It was established that male patients with breast cancer showed significant disorders of the functional state of the pituitary, adrenal cortex and gonads. This may be significant for the efficacy of combined treatment.

[Functional state of the hypophysis, adrenal cortex and gonads in patients with gynecomastia]. / Funktsional'noe sostoianie gipofiza, kory nadpochechnikov i gonad u bol'nykh ginekomastiei.

Patients with gynecomastia revealed simultaneous increase in blood levels of estrogens, glucocorticoids and pituitary (first of all, gonadotropic) hormones associated with a decrease in blood-androgen concentration. Such combination of disturbances may account for hyperplasia of the male breast.

[The effect of age-related changes on the hypophyseo-adrenal and sympathetico-adrenal systems in patients with cancer of the digestive tract and lungs]. / Vliianie vozrastnykh izmenenii na gipofizarno-nadpochechnikovuiu i simpato-adrenalovuiu sistemy u bol'nykh rakom pishchevaritel'nogo kanala i legkikh.

An analysis of the activity of pituitary-adrenal and sympathetic-adrenal systems in patients with cancer of the digestive canal and lungs with consideration of age, sex and stage of the disease indicates that not age but the development of the tumour process is the leading factor in disorders of the neuroendocrine homeostasis. Therapeutic measures are discussed.

[Prognostic value of urinary levels of corticosteroids and catecholamines in patients with cancer of the transverse colon and rectum]. / Prognosticheskoe znachenie soderzhaniia v moche kortikosteroidov i katekholaminov u bol'nykh rakom obodochnoi i priamoi kishki.

Long-term results of treatment of colorectal cancer versus levels of 17-ketosteroids, 17-ketogenic steroids, adrenaline and norepinephrine in diurnal urine were evaluated in 116 patients. Normal or slightly elevated urine-corticosteroid and catecholamine levels were shown to correlate with good prognosis, particularly, in cases with normal excretion of all (or almost all) hormones studied and their normal ratios.

[Correction of the function of the sympathoadrenal system in the surgical and combined treatment of patients with cancer of the esophagus and stomach]. / Korrektsiia funktsii simpatoadrenalovoi sistemy pri khirurgicheskom i kombinirovannom lechenii bol'nykh rakon pishchevoda i zheludka.

The study was concerned with the functional condition of the sympatho-adrenal system in 80 patients with cancer of the esophagus and proximal part of the stomach who had undergone surgery and received combined treatment, coupled with measures aimed at correcting the sympatho-adrenal system in some cases. Treatment with phenformin, decaris and S-methylmethionine was shown to produce a favorable effect on certain stages of catecholamine synthesis and metabolism as well as on immunologic indexes and hemodynamic stability.

[The pituitary-adrenal system in cancer of the esophagus and stomach]. / Gipofizatno-nadpochechnikovaia sistema pri rake pishchevoda i heludka.

Excretion of corticosteroids, levels of 11-OCS in blood plasma, tests with dexasone and clinical evaluation of the pituitary-adrenal system function were studied in 354 patients with carcinomas of the esophagus and proximal stomach division. Some degree of hypocorticism with a decrease in the 11-OCS level in blood plasma and lowered excretion of corticosteroids in the urine were found in 71% of patients. These variations are accompanied by lowered hypothalamus sensitivity to dexasone. Similar disturbances though less pronounced are determined in the precancerous states and in the remote periods after operation.

[Impaired function of the sympatho-adrenal system in patients with esophageal and stomach cancer]. / Narushenie funktsii simpatoadrenalovoi sistemy u bol'nykh rakom pishchevoda i zheludka.

Patients with esophageal and gastric cancer show a decreased diurnal excretion of DOPA matched by a considerable drop in the urine levels of catecholamines and their main metabolites as compared with healthy subjects. This points to a severe inhibition of synthesis and metabolism of catecholamines at all stages. Tumor process progression is accompanied by an ever-increasing inhibition of sympatho-adrenal system function. Simultaneously, disorders develop in the functioning of the cholinergic structures of the nervous system.